Predicted Off-targets for siRNA "aaagaaguacuaucagauugaccaacc" Using siRNA SpecificityServer

Specificity Server [Result page v1.0]


SpecificityServer - An siRNA design program for determining siRNA specificity

Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands

9 hits were found for ACUAUCAGAUUGACCAACC (2 guide strand hits and 7 sense strand hits.)

Hits to Guide strand

AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
NM_014661 FAM53B 693 711 cucuUCAGAUUGACCAACC30 4 15 bc false   <90%   5740
NM_020794 LRRC7 3110 3128 uCUAUCAGuUUGACCAAag30 4 8 c false   <90%   6313


Hits to Sense strand








AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
NM_021737 CLCN6 1625 1643 GGaUGGUCAAUCUGAUAaU77 2 14 c false   <90%   3858
NM_021735 CLCN6 1661 1679 GGaUGGUCAAUCUGAUAaU77 2 14 c false   <90%   3894
NM_021736 CLCN6 1760 1778 GGaUGGUCAAUCUGAUAaU77 2 14 c false   <90%   3993
NM_001286 CLCN6 3308 3326 GGaUGGUCAAUCUGAUAaU77 2 14 c false   <90%   5541
NM_173644 C20orf197 1498 1516 ucUUGuUCAAUCUGAUAGg18 4 12 bc false   <90%   2732
XM_938907 KIAA1240 1781 1799 GGUUGGUgAAUCUGAaAGg6 3 7 c false   <90%   8103
XM_039676 KIAA1240 1781 1799 GGUUGGUgAAUCUGAaAGg6 3 7 c false   <90%   8103


3 prime UTR Seed matches

Hits to same gene : 0
Hits to other genes : 1094
View matched accessions

3 prime seed match details

AccessionGene nameHits to this gene
NM_152624DCP23
NM_001776ENTPD13
NM_0010981753
NM_001009555SH3D193
NM_173552C3orf583
NM_207361FREM23
NM_006731FCMD2
NM_001079802FCMD2
NM_001077445PHF162
NM_014735PHF162
NM_003590CUL32
NM_001042599ERBB42
NM_005235ERBB42
NM_004171SLC1A22
NM_020810TRMT52
NM_194298SLC16A92
NM_021961TEAD12
NM_182484BAGE52
NM_181704BAGE42
NM_004229CRSP22
NM_001001874TPD52L32
NM_199246CCNG12
NM_004060CCNG12
NM_005233EPHA32
NM_001010000ARHGAP282
NM_001010883FAM102B2
NM_014904RAB11FIP22
NM_033512TSPYL52
NM_001032288UBE2V12
NM_017577GRAMD1C2
NM_002645PIK3C2A2
NM_016599MYOZ22
NM_016531KLF32
NM_152291MUC72
NM_001012659ARGFX2
XM_934619LOC2845772
XM_945015LOC2845772
NM_178818CMTM42
NM_181521CMTM42
NM_001609ACADSB2
NM_001030060SAMD52
NM_176081TRDMT12
NM_004412TRDMT12
NM_176083TRDMT12
NM_006581FUT92
NM_004296RGS62
NM_007220CA5B2
NM_012461TINF22
NM_152395NUDT162
NM_014906PPM1E2
NM_145167PIGM2
NM_004586RPS6KA32
NM_030625CXXC62
NM_173536GABRG12
NM_004678BPY22
NM_001002760BPY2B2
NM_001002761BPY2C2
NM_033114ZCRB12
NM_013943CLIC41
NM_016093RPL26L11
NM_020424LYRM11
NM_014157CCDC1131
NM_017736THUMPD11
NM_006751SSFA21
NM_024800NEK111
NM_001040285PAPD51
NM_001040284PAPD51
NM_002076GNS1
NM_020768KCTD161
NM_015045WAPAL1
NM_020917ZFP141
NM_006306SMC1A1
NM_006583RRH1
NM_002223ITPR21
NM_014612FAM120A1
XM_378742FLJ459941
XM_943185FLJ459941
NM_0178921
NM_177965C8orf371
NM_194314ZBTB411
NM_002835PTPN121
NM_018214LRRC11
NM_016348C5orf41
NM_032385C5orf41
NM_032601MCEE1
NM_001002844SUHW41
NM_019000FLJ201521
NM_001034850FLJ201521
XM_934931LOC4418761
XM_946278LOC4418761
XM_001134045LOC7299031
XM_001133484LOC7314981
NM_144721THAP61
NM_005578LPP1
NM_004742MAGI11
XM_001131501FLJ902311
NM_001080407GLB1L31
NM_000240MAOA1
NM_152458ZNF7851
NM_201564SYCE11
NM_130784SYCE11
NM_001682ATP2B11
NM_001001323ATP2B11
NM_173538CNBD11
NM_004745DLGAP21
NM_032961PCDH101
NM_002188IL131
NM_007146VEZF11
NM_018379FAM63A1
NM_001568EIF3S61
NM_207411XKR51
NM_031475ESPN1
NM_001032731OAS21
NM_005584MAB21L11
NM_001409MEGF61
NM_181789GLDN1
NM_006544EXOC51
NM_001037293PALM21
NM_053016PALM21
NM_002232KCNA31
NM_021003PPM1A1
NM_177952PPM1A1
NM_002857PEX191
NM_014041SPCS11
NM_199000LHFPL31
NM_015058KIAA05641
NM_025134CHD91
XM_941706LOC6490091
NM_181523PIK3R11
NM_181504PIK3R11
NM_181524PIK3R11
NM_001076786QSER11
NM_019072SGTB1
NM_015268DNAJC131
NM_005603ATP8B11
NM_003972BTAF11
NM_000446PON11
NM_003184TAF21
NM_173500TTBK21
NM_004852ONECUT21
NM_014504RABGEF11
NM_001187BAGE1
NM_014691AQR1
NM_016823CRK1
NM_005206CRK1
NM_177538CYP20A11
NM_002454MTRR1
NM_024010MTRR1
NM_018299UBE2W1
NM_001001481UBE2W1
NM_1779662'-PDE1
NM_032521PARD6B1
NM_017658KLHL281
NM_015346ZFYVE261
NM_020826SYT131
NM_017593BMP2K1
NM_138571HINT31
NM_016248AKAP111
NM_001010853ACY1L21
NM_052962IL22RA21
NM_181309IL22RA21
NM_181310IL22RA21
NM_005413SIX31
NM_001188BAK11
NM_005069SIM21
NM_025040ZNF6141
NM_033211C5orf301
NM_198947FAM111B1
NM_001080745DGKG1
NM_001346DGKG1
NM_001080744DGKG1
NM_0010996451
NM_004747DLG51
NM_173494CXorf411
NM_020375C12orf51
NM_1827627A51
NM_015291DNAJC161
NM_012257HBP11
NM_033060KRTAP4-101
NM_000399EGR21
NM_024533CHST51
NM_015347RIMBP21
NM_001039395C9orf681
NM_018945PDE7B1
NM_002235KCNA61
NM_145808MTPN1
XM_001127087FLJ439501
NM_013995LAMP21
NM_006482DYRK21
NM_003583DYRK21
NM_174911FAM84B1
NM_005924MEOX21
NM_006827TMED101
NM_173545C2orf131
NM_022845CBFB1
NM_001755CBFB1
NM_024757EHMT11
XM_943454LOC6465881
XM_935989LOC6465881
XM_933738LOC6465881
NM_144508CASC51
NM_170589CASC51
NM_006054RTN31
NM_201429RTN31
NM_201428RTN31
NM_201430RTN31
NM_052885SLC2A131
NM_001077440BCLAF11
NM_001077441BCLAF11
NM_014739BCLAF11
NM_020802KIAA13771
XM_001131164LOC7309711
NM_001159AOX11
NM_173529C18orf541
NM_199053FLJ127161
NM_021942FLJ127161
NM_178470WDR40B1
NM_000933PLCB41
NM_182797PLCB41
NM_022073EGLN31
NM_005431XRCC21
NM_005860FSTL31
NM_015382HECTD11
NM_006402HBXIP1
NM_001013403LOC3474871
NM_005406ROCK11
XM_001125808LOC7277581
NM_183059RD31
XM_001131019LOC7318461
XM_001131201LOC7297511
NM_015554GLCE1
NM_012243SLC35A31
NM_016591GCNT41
NM_153000APCDD11
NM_006424SLC34A21
NM_173607C14orf241
NM_001079519C14orf241
NM_153018ZFP31
NM_001001132ITSN11
NM_001655ARCN11
NM_178125TRIM501
NM_021927GUF11
NM_016125LOC511361
NM_145165CHURC11
NM_015288PHF151
NM_007010DDX521
NM_152300DDX521
NM_005292GPR181
NM_0010982001
NM_199144UBE2V11
NM_003349Kua-UEV1
NM_199203Kua-UEV1
NM_021988UBE2V11
NM_022442UBE2V11
NM_018318CCDC911
NM_173547TRIM651
NM_153027FLJ316591
NM_014310RASD21
NM_001003760KLHL311
NM_015206KIAA10241
NM_018409LRP2BP1
NM_024042METRN1
NM_017812CHCHD31
NM_003925MBD41
NM_006547IGF2BP31
NM_030648SETD71
NM_017738C9orf391
NM_024592SRD5A2L1
NM_024345WDR321
NM_001124ADM1
NM_000434NEU11
NM_016081PALLD1
NM_177433MAGED21
NM_201222MAGED21
NM_014599MAGED21
NM_006895HNMT1
XM_935369LOC6463291
XM_944654LOC6487161
XM_935966LOC6417981
NM_052867NALCN1
NM_020186ACN91
NM_033426KIAA17371
XM_942991LOC6429341
NM_014352POU2F31
NM_024577SH3TC21
NM_001042690LOC3452221
NM_144712SLC23A31
NM_018840C20orf241
NM_199483C20orf241
NM_017848FAM120C1
NM_152476ZNF5601
NM_206925CA121
NM_001218CA121
NM_152547BTNL91
XM_001129853LOC7292921
NM_005213CSTA1
NM_001012393OPCML1
NM_002545OPCML1
NM_001383DPH11
NM_080822OVCA21
NM_001079669TMTC41
NM_032813TMTC41
NM_019022TXNDC101
NM_000232SGCB1
NM_019114EPB41L4B1
NM_003783B3GALT21
NM_018712ELMOD11
NM_020357PCNP1
NM_004905PRDX61
NM_003149STAC1
NM_019018FAM105A1
NM_003161RPS6KB11
NM_152289ZNF5611
NM_003505FZD11
NM_001040272C9orf941
NM_173645FLJ373571
NM_003626PPFIA11
NM_006298ZNF1921
NM_139279MCFD21
NM_198715PTGER31
NM_001039844ACBD71
NM_000963PTGS21
NM_030883OR2H11
NM_000778CYP4A111
NM_005504BCAT11
NM_001025436SPAG161
NM_031911C1QTNF71
NM_021183RAP2C1
NM_000885ITGA41
NM_005187CBFA2T31
NM_175931CBFA2T31
NM_0010982721
NM_002130HMGCS11
NM_020781ZNF3981
NM_170686ZNF3981
NM_0010937261
NM_0010854861
NM_005410SEPP11
NM_016083CNR11
NM_033181CNR11
NM_198847FAM111A1
NM_022074FAM111A1
NM_001001709FLJ455371
NM_014953DIS31
NM_014048MKL21
NM_006141DYNC1LI21
NM_003982SLC7A71
NM_006729DIAPH21
NM_001017922ERMAP1
NM_018538ERMAP1
NM_030641APOL61
NM_012137DDAH11
NM_002500NEUROD11
NM_138991BACE21
NM_138992BACE21
NM_012105BACE21
NM_001025266LOC2853821
NM_152519FLJ238611
NM_014911AAK11
NM_002267KPNA31
NM_020717SHROOM41
NM_018364RSBN11
NM_006646WASF31
NM_012381ORC3L1
NM_181837ORC3L1
NM_032087PCDHGA71
NM_021007SCN2A1
NM_001040142SCN2A1
NM_001040143SCN2A1
NM_000787DBH1
NM_0011046471
NM_018155SLC25A361
NM_018130SHQ11
NM_022716PRRX11
NM_006902PRRX11
NM_172071RC3H11
NM_153635CPNE91
NM_032826SLC35B41
NM_198098AQP11
XM_940667SDC31
NM_014309RBM91
NM_001082576RBM91
NM_001082577RBM91
NM_001031695RBM91
NM_001082578RBM91
NM_001082579RBM91
NM_020377CYSLTR21
NM_017987RUFY21
NM_005385NKTR1
NM_021649TICAM21
NM_000836GRIN2D1
NM_001160APAF11
NM_181868APAF11
NM_181869APAF11
NM_013229APAF11
NM_181861APAF11
XM_001128847LOC7289421
XM_001131211LOC7319061
XM_001126328LOC3881521
XM_001125719LOC7277511
XM_001125942LOC7278491
XM_925864LOC6423251
XM_001130526LOC7286361
XM_001130555LOC7286361
XM_001130543LOC7286361
XM_001130536LOC7286361
XM_001129399LOC6423461
XM_928707LOC6423461
XM_925879LOC6423461
NM_001007538TMEM461
NM_001421ELF41
NM_182972IRF2BP21
NM_001077397IRF2BP21
NM_006918SC5DL1
NM_001024956SC5DL1
NM_133265AMOT1
XM_209363SIRPB21
XM_939897SIRPB21
NM_015275KIAA10331
NM_207467FLJ355301
NM_178353LCE1E1
NM_002009FGF71
NM_014663JMJD2A1
NM_033240PML1
NM_017602OTUD51
NM_001903CTNNA11
NM_007351MMRN11
XM_001128488LOC7282541
XM_001127513LOC7305811
NM_138400NOM11
NM_014648DZIP31
XM_001132703FLJ410471
XM_001133280FLJ410471
NM_139072DNER1
NM_003012SFRP11
NM_152551C6orf1511
NM_018963BRWD11
NM_0229061
XM_001130018LOC7315501
XM_001128522LOC7288001
NM_033437PDE5A1
NM_001083PDE5A1
NM_033430PDE5A1
NM_001077484SLC38A11
NM_030674SLC38A11
NM_207407TMPRSS11F1
NM_013281FLRT31
NM_198391FLRT31
NM_020373TMEM16B1
NM_032979DTNA1
NM_032978DTNA1
NM_001391DTNA1
NM_032981DTNA1
NM_024627C22orf291
NM_020927KIAA15761
NM_001821CHML1
NM_001712CEACAM11
NM_001024912CEACAM11
NM_001002906XKRY21
NM_004677XKRY1
NM_005188CBL1
XM_001130573LOC7317271
XM_001130089LOC7293761
NM_007084SOX211
NM_004936CDKN2B1
NM_078487CDKN2B1
NM_0010099841
NM_014607UBXD21
NM_001080523ARRDC51
XM_940152LOC6454321
NM_033064ATCAY1
NM_003496TRRAP1
NM_153343ENPP61
NM_000139MS4A21
NM_015328KIAA08281
XM_379228LOC4010931
XM_944985LOC4010931
NM_006469IVNS1ABP1
NM_198514NHLRC21
NM_001701BAAT1
NM_000582SPP11
NM_001040058SPP11
NM_001040060SPP11
NM_006912RIT11
NM_153645NUP501
NM_007172NUP501
NM_005313PDIA31
NM_002729HHEX1
XM_929032LOC6460711
XM_001125928LOC2838041
XM_001126277LOC6489131
NM_030791SGPP11
NM_021045ZNF2481
NM_017734PALMD1
NM_018159NUDT111
NM_004685MTMR61
NM_052918SORCS11
NM_001013031SORCS11
NM_018327SPTLC31
NM_013437LRP121
NM_017554PARP141
NM_015236LPHN31
NM_024616C3orf521
XM_001133925GRIP11
XM_001133901GRIP11
XM_001133924GRIP11
XM_001133902GRIP11
NM_198493ANKRD451
NM_021229NTN41
XM_926142LOC6426911
NM_006682FGL21
NM_201544LGALS81
NM_006499LGALS81
NM_201545LGALS81
NM_201543LGALS81
NM_001039396MPEG11
NM_000126ETFA1
NM_030759NRBF21
XM_001127763LOC7308201
XM_001127798LOC7283811
NM_152275TTC30A1
NM_176825SULT1C21
NM_001056SULT1C21
NM_178815ARL5B1
NM_001004329DBX21
NM_006252PRKAA21
NM_007032TRIOBP1
NM_001039141TRIOBP1
NM_001039580MAP91
NM_007137ZNF811
NM_0011031491
NM_018215FLJ107811
NM_002667PLN1
NM_014381MLH31
NM_001040108MLH31
NM_014810CEP3501
NM_016277RAB231
NM_183227RAB231
NM_006997TACC21
NM_206860TACC21
NM_206862TACC21
NM_206861TACC21
NM_007283MGLL1
NM_001003794MGLL1
NM_198465NRK1
NM_005941MMP161
NM_014233UBTF1
NM_001076683UBTF1
NM_001076684UBTF1
NM_004670PAPSS21
NM_001015880PAPSS21
XM_496034LOC6537171
NM_016133INSIG21
NM_019845RPRM1
NM_173167UNC45B1
NM_001033576UNC45B1
NM_014746RNF1441
NM_033221TRIM141
NM_014788TRIM141
NM_003099SNX11
NM_152826SNX11
NM_148955SNX11
NM_002109HARS1
NM_015576ERC21
NM_032174TOMM40L1
NM_052846EMILIN31
NM_052870SNAG11
NM_032968PCDH11X1
NM_032969PCDH11X1
NM_032973PCDH11Y1
NM_001008537KIAA20221
NM_170601SIAE1
NM_016141DYNC1LI11
NM_0321731
XM_944029LOC6479791
NM_0011128001
NM_021097SLC8A11
NM_0011128011
NM_0011128021
XM_496436MIA31
XM_932541MIA31
XM_932548MIA31
NM_005955MTF11
XM_926521LOC6531371
XM_928267LOC6532701
NM_001071775LOC4401451
NM_019014POLR1B1
NM_012097ARL5A1
NM_001037174ARL5A1
NM_177985ARL5A1
XM_001130238LOC7282831
XM_001132687LOC7310871
NM_198595AFAP11
NM_021638AFAP11
NM_0010996781
NM_004589SCO11
NM_194071CREB3L21
NM_001017370LOC3879211
NM_001012754LOC3879211
NM_014677RIMS21
NM_0011001171
NM_032833PPP1R15B1
NM_025264THUMPD21
NM_007153ZNF2081
NM_018061PRPF38B1
NM_182625FLJ408691
NM_024583SCRN31
NM_020124IFNK1
NM_017516RAB391
NM_024411PDYN1
NM_003632CNTNAP11
NM_0011024701
NM_207359GADL11
NM_203403C9orf1501
NM_0208271
NM_015059TLN21
NM_213605ZNF5171
NM_213589RAPH11
NM_004513IL161
NM_172217IL161
NM_001025249DUT1
NM_001025248DUT1
NM_001948DUT1
NM_005665EVI51
NM_153214FLJ374401
NM_014967MTMR151
NM_001007233ERCC81
NM_000082ERCC81
NM_005595NFIA1
NM_006283TACC11
NM_020346SLC17A61
NM_182521ZSWIM21
NM_006606RBBP91
NM_014141CNTNAP21
XM_927936LOC6448411
XM_935685LOC6419331
XM_938176LOC6448411
NM_016436PHF201
XM_945436LOC4009581
XM_379100LOC4009581
NM_032047B3GNT51
NM_153711FAM26E1
NM_016052RRP151
NM_018841GNG121
NM_178120DLX11
NM_001038493DLX11
NM_020353PLSCR41
NM_004859CLTC1
NM_007218RNF1391
NM_030636KIAA17061
NM_152424FAM123B1
NM_007214SEC631
XM_001125756LOC7277881
XM_001125734LOC7277811
NM_006092NOD11
NM_032457PCDH71
NM_198524TEX91
NM_007325GRIA31
NM_000828GRIA31
NM_005509DMXL11
XM_001126738LOC7281661
XM_001126761LOC7306781
NM_139312YME1L11
NM_014263YME1L11
NM_001518GTF2I1
XM_001133646LOC7324371
NM_032999GTF2I1
NM_033000GTF2I1
NM_033001GTF2I1
NM_002398MEIS11
NM_018323PI4K2B1
NM_022834VWA11
NM_199121VWA11
NM_001003652SMAD21
NM_005901SMAD21
NM_172177MRPL421
NM_172178MRPL421
NM_014050MRPL421
NM_201520SLC25A351
NM_000268NF21
NM_016418NF21
NM_181832NF21
NM_181828NF21
NM_181829NF21
NM_181833NF21
NM_181830NF21
NM_145168HSPC1051
NM_001364DLG21
NM_144499GNAT11
NM_001002909GPATCH81
NM_014615KIAA01821
NM_000868HTR2C1
NM_006374STK251
NM_024103SLC25A231
NM_182579C1orf1571
NM_153365TAPT11
NM_152470RNF1651
NM_033637BTRC1
NM_003939BTRC1
NM_002836PTPRA1
NM_080840PTPRA1
NM_080841PTPRA1
NM_0146921
NM_032020FUCA21
NM_005840SPRY31
NM_014763MRPL191
NM_021111RECK1
NM_020125SLAMF81
NM_133496SLC30A71
NM_005158ABL21
NM_007314ABL21
NM_0011001081
NM_014069PSORS1C21
NM_006699MAN1A21
NM_018492PBK1
NM_016079VPS241
NM_001005753VPS241
NM_153456HS6ST31
NM_002731PRKACB1
NM_182948PRKACB1
NM_012224NEK11
NM_138270ATRX1
NM_000489ATRX1
XM_001128623LOC7288491
NM_198124CSMD31
NM_198123CSMD31
NM_052900CSMD31
NM_032124HDHD21
NM_005780LHFP1
NM_198570VWC21
NM_001039840CHIC11
NM_017596KIF21B1
NM_144625WDR641
NM_001011538LOC4021761
NM_133367PAQR81
NM_203446SYNJ11
XM_931447SETD51
XM_944298SETD51
XM_944295SETD51
XM_931444SETD51
NM_0011006261
NM_006459ERLIN11
NM_005226EDG31
NM_152420C9orf411
NM_001039360ZBTB7C1
NM_198484ZNF6211
NM_0010984141
XM_927029PPP1R3E1
XM_940069PPP1R3E1
NM_005056JARID1A1
NM_001042603JARID1A1
NM_016297PCYOX11
XM_001126151LOC7278581
XM_001130844LOC7287641
NM_014732KIAA05131
XM_941807NHSL11
XM_496826NHSL11
NM_0010984021
NM_0010984031
NM_020727ZNF2951
NM_182757IBRDC21
NM_003954MAP3K141
NM_002463MX21
NM_000555DCX1
NM_178152DCX1
NM_178153DCX1
NM_178151DCX1
NM_003439ZKSCAN11
NM_024944CHODL1
NM_015020PHLPPL1
NM_025265TSEN21
NM_022168IFIH11
NM_007106UBL31
NM_002196INSM11
NM_000194HPRT11
NM_003305TRPC31
NM_174940TMEM801
NM_001042463TMEM801
NM_000813GABRB21
NM_021911GABRB21
NM_002575SERPINB21
NM_001018075NR3C11
NM_001018077NR3C11
NM_001018076NR3C11
NM_001018074NR3C11
NM_001024094NR3C11
NM_000176NR3C11
NM_175736FMNL31
NM_198900FMNL31
NM_012234RYBP1
NM_015721GEMIN41
NM_025214CCDC681
NM_004666VNN11
NM_015458MTMR91
NM_173497HECTD21
NM_173084TRIM591
NM_017833C21orf551
NM_001040192C21orf551
NM_033402LRRCC11
NM_001077501LRRCC11
NM_0010987211
NM_0010987221
NM_004485GNG41
NM_080661GLYATL11
NM_024624SMC61
NM_001547IFIT21
NM_023110FGFR11
NM_023106FGFR11
NM_015850FGFR11
NM_023111FGFR11
NM_023105FGFR11
NM_001982ERBB31
NM_173667FLJ375431
NM_007211RASSF81
NM_024871MAP6D11
NM_138473SP11
NM_012092ICOS1
NM_000321RB11
NM_002404MFAP41
NM_001029863C6orf1201
NM_152556FLJ318181
NM_007173PRSS231
NM_015040PIP5K31
NM_001008390CGGBP11
NM_003663CGGBP11
NM_004337OSGIN21
NM_004612TGFBR11
NM_001624AIM11
NM_003269NR2E11
NM_033281MRPS361
NM_014930ZNF5101
NM_005772RCL11
NM_032856WDR731
NM_005437NCOA41
XM_049078KIAA12391
XM_940885KIAA12391
NM_001008723C10orf801
NM_001040709SYPL21
NM_005962MXI11
NM_001008541MXI11
NM_130439MXI11
NM_004898CLOCK1
NM_024512LRRC21
NM_024750LRRC21
NM_018933PCDHB131
NM_001001346CLDN201
NM_016608ARMCX11
NM_001204BMPR21
NM_198578LRRK21
NM_182589HTR3E1
NM_001010874SRD5A2L21
XM_931376SETD51
XM_944260SETD51
XM_931478SETD51
XM_944280SETD51
XM_931417SETD51
XM_944276SETD51
XM_939712SETD51
NM_001080517SETD51
NM_194285SPTY2D11
NM_001455FOXO3A1
NM_201559FOXO3A1
NM_001001668ZNF4701
NM_001527HDAC21
NM_001042413GLIS31
NM_152629GLIS31
NM_006504PTPRE1
NM_130435PTPRE1
NM_014829DDX461
NM_152265BTF3L41
NM_152745NXPH11
NM_198151LOC2530121
NM_001039372LOC2530121
NM_018361AGPAT51
NM_001010846SHE1
NM_015904EIF5B1
NM_003117SPAM11
NM_001040214TCBA11
NM_138959VANGL11
NM_032208ANTXR11
NM_0011107811
NM_006546IGF2BP11
NM_019863F81
NM_000132F81
NM_199464KCNRG1
NM_025009CEP1351
NM_152682RWDD4A1
NM_005683GPR551
NM_001011713NAT121
NM_152874FAS1
NM_152876FAS1
NM_152877FAS1
NM_152872FAS1
NM_152873FAS1
NM_152875FAS1
NM_002906RDX1
NM_173827COX181
NM_000329RPE651
NM_024947PHC31
NM_007124UTRN1
NM_198281GPRIN31
NM_007347AP4E11
XM_001127274SMYD21
NM_006726LRBA1
NM_004402DFFB1
NM_005420SULT1E11
NM_023073FLJ132311
NM_005202COL8A21
NM_173570ZDHHC231
NM_001012981ZKSCAN21
NM_031366COL4A31
NM_000091COL4A31
NM_031365COL4A31
NM_031362COL4A31
NM_006951TAF51
NM_007086WDHD11
NM_001008396WDHD11
NM_014044UNC501
NM_020718USP311
NM_001080396LOC7282151
XM_001133620LOC7318951
NM_022497MRPS251
NM_012329MMD1
NM_001010934HGF1
NM_003430ZNF911
NM_000926PGR1
NM_004650PNPLA41
XM_001134421C1orf1521
NM_032280ZCCHC91
NM_006047RBM121
NM_152838RBM121
NM_015564LRRTM21
NM_033631LUZP11
NM_080927DCBLD21
NM_015317PUM21
NM_002971SATB11
NM_152850PIGO1
NM_032634PIGO1
NM_0011025751
NM_031844HNRPU1
NM_004501HNRPU1
NM_032182KIAA01571
NM_207582HERV-FRD1
NM_016530RAB8B1
NM_000913OPRL11
NM_182647OPRL11
NM_0010839621
NM_003199TCF41
NM_182482BAGE21
NM_182481BAGE31
NM_033506FBXO241
NM_032873STS-11
NM_001013640LOC3885031
NM_024557RIC31
NM_001025433NQO11
NM_000903NQO11
NM_001025434NQO11
NM_198459DENND2C1
NM_033439IL331
XM_373030LOC2855561
XM_939505LOC2855561
NM_015352POFUT11
NM_015477SIN3A1
NM_019118C1orf911
NM_014946SPAST1
NM_199436SPAST1
NM_182508C13orf301
NM_000647CCR21
XM_001133430LOC7292301
XM_934188LOC6538011
NM_0010988131
NM_0151461
NM_0010988121
NM_133504DCN1
NM_133506DCN1
NM_133503DCN1
NM_001920DCN1
NM_133507DCN1
NM_133505DCN1
NM_015455CNOT61
NM_018427RRN31
NM_004866SCAMP11
NM_005295GPR221
NM_020773TBC1D141
NM_015691WWC31
NM_007149ZNF1841
NM_002834PTPN111
NM_001004125TUSC11
NM_019069WDR5B1
NM_020774MIB11
NM_003128SPTBN11
NM_178586PPP2R5C1
NM_002719PPP2R5C1
NM_020463SMEK21
NM_000944PPP3CA1
NM_152388ALS2CR41
NM_001044385ALS2CR41
NM_021101CLDN11
NM_014880CD3021
NM_004508IDI11
NM_001042399CCDC411
NM_016122CCDC411
NM_015530GORASP21
NM_006650CPLX21
NM_001008220CPLX21
NM_021201MS4A71
NM_206938MS4A71
NM_206940MS4A71
NM_206939MS4A71
NM_021982SEC24A1
NM_014892RBM161
NM_172364CACNA2D41
XM_496115LOC4403381
XM_940659LOC4403381
NM_005433YES11
NM_145273CD300LG1
NM_152729NT5DC11
NM_139283PPTC71
NM_012430SEC22A1
NM_021218C9orf801
NM_0011008291
NM_147166AKAP91
NM_022784IQCH1
NM_212558LOC4014981
NM_001030006AP2B11
NM_001282AP2B11
NM_001007257PRDM21
NM_015866PRDM21
NM_020337ANKRD501
NM_198389PDPN1
NM_006474PDPN1
NM_001006624PDPN1
NM_001006625PDPN1
NM_000460THPO1
NM_015147CEP681
NM_014809KIAA03191
NM_021233DNASE2B1
NM_058248DNASE2B1
NM_000369TSHR1
NM_019001XRN11
NM_001042604XRN11
NM_024989PGAP11
NM_024751GSTCD1
NM_138335GNPDA21
NM_001858COL19A11
NM_153262SYT141
NM_007345ZNF2361
NM_006572GNA131
NM_001024847TGFBR21
NM_003242TGFBR21
NM_002357MXD11
NM_015051TXNDC41
NM_001037339PDE4B1
NM_002600PDE4B1
NM_001037340PDE4B1
NM_001037341PDE4B1
NM_033397KIAA17541
NM_176889TAS2R491
NM_022763FNDC3B1
NM_015192PLCB11
NM_182734PLCB11
NM_015879ST8SIA31
NM_015660GIMAP21
NM_172240WDR51B1
NM_014005PCDHA91
NM_078469BCCIP1
NM_173602DIP2B1
NM_002163IRF81
NM_005739RASGRP11
NM_005759ABI21
XM_001126750ABI21
NM_133493CD1091
NM_001297CNGB11
XM_001133760ZNF5191
NM_001201BMP31
NM_178445CCRL11
NM_016557CCRL11
NM_006466POLR3F1
NM_001077700MIER11
NM_001077701MIER11
NM_020948MIER11
NM_173559FLJ257911
NM_138287DTX3L1
NM_001032282KLF101
NM_005655KLF101
NM_003941WASL1
NM_0177821
NM_001040451RUFY11
NM_025158RUFY11
NM_001040452RUFY11
NM_007038ADAMTS51
NM_1449931
NM_020148SPIRE11
NM_005859PURA1

Immunostimulatory motif search results


UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).

GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).



Settings used for search

Scores were calculated using scoring scheme SS1
Using AVG scoring mode.

Hit scores

ParameterSpec. Score cut-offColourComment
Hit to same geneColourHits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit.
High scoring hit≥80ColourHits are very likely to cause an off-target effect
Medium scoring hit<80¸≥40ColourHits with a small chance of causing an off target effect.
Low scoring hitColourHits very unlikely to cause an off-target effect

The mismatches are colour coded based on the weighting of that position.

Cut-offColour
MatchColour
Wobble base pairs (G-U)Colour
Critical bases (none)Colour
0.6 bitsColour
0.4 bitsColour
0.2 bitsColour

About SpecificityServer

SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).

Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer


For more details about the algorithm "siRNA specificity searching incorporating mismatch tolerance data" Bioinformatics (2008) 24 (10): 1316-1317