Predicted Off-targets for siRNA "ugcacgguguugacugauuuc" Using siRNA SpecificityServer

Specificity Server [Result page v1.0]


SpecificityServer - An siRNA design program for determining siRNA specificity

Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands

5 hits were found for CACGGUGUUGACUGAUUUC (0 guide strand hits and 5 sense strand hits.)

Hits to Sense strand






AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
NR_001536 TTTY2 1528 1546 aAAAUCAGUCAACAaCaUG49 3 13 c false   <90%   3177
NM_003292 TPR 5358 5376 GAAAUaAGUCAACACCcaG10 3 10 c false   <90%   9727
XM_376903 KIAA0674 537 555 GAAAUCAGgCAACACCaaa8 4 8 c false   <90%   4588
XM_936355 KIAA0674 537 555 GAAAUCAGgCAACACCaaa8 4 8 c false   <90%   4588
NM_000686 AGTR2 2027 2045 GAAAUCAGUaAACACuGUG-8 2 9 c false   <90%   2448


3 prime UTR Seed matches

Hits to same gene : 0
Hits to other genes : 1007
View matched accessions

3 prime seed match details

AccessionGene nameHits to this gene
NM_003681PDXK4
NM_001167BIRC43
XM_001130364LOC7284173
XM_001130184LOC7308433
NM_006675TSPAN93
XM_379273FLJ141863
XM_001130790KIAA18813
NM_001080400KIAA18813
NM_012402ARFIP22
NM_001037165FOXK12
NM_133375DIS3L2
NM_001005502CPM2
NM_198320CPM2
NM_001874CPM2
NM_015147CEP682
NM_003676DEGS12
NM_152362TNFAIP8L12
NM_198179GPR1032
NM_024875SYNPO2L2
NM_022103ZNF6672
NM_018141MRPS102
NM_138801GALM2
NM_005975PTK62
NM_0010996782
NM_024804ZNF6692
NM_015092SMG12
NM_152411ZNF7862
NM_181725METTL2A2
NM_001005372METTL2A2
NM_032048EMILIN22
NM_020133AGPAT42
NM_004808NMT22
NM_017925DENND4C2
XM_001133581LOC7295402
NM_020251ARRB12
NM_004041ARRB12
NM_024841FLJ142132
NM_024963FBXL182
XM_933539MGC30322
XM_944529MGC30322
NM_005044PRKX2
NM_203327SLC23A22
NM_005116SLC23A22
NM_022755IPPK2
NM_003644GAS72
NM_201432GAS72
NM_201433GAS72
NM_001918DBT2
NM_001012706LOC2835512
NM_014903NAV32
NM_006869CENTA12
XM_940609FLJ250762
XM_059689FLJ250762
NM_001013640LOC3885032
NM_001029861NANOS22
NM_001040441ZBTB82
XM_001124597LOC7304112
XM_933614LOC6464342
XM_929242LOC6463012
NM_004079CTSS2
NM_138568EXOC3L22
NM_021116ADCY12
NM_014938MLXIP2
NM_002193INHBB2
XM_933796SLC45A42
NM_003395WNT9A2
NM_002360MAFK2
NM_017883WDR131
NM_015458MTMR91
NM_017621ALKBH41
NM_018992KCTD51
NM_016261TUBD11
NM_006252PRKAA21
NM_032105PPP1R12B1
NM_002481PPP1R12B1
NM_198276TMEM171
NM_005158ABL21
NM_007314ABL21
NM_0011001081
NM_030779KCNH61
XM_001124844LOC7304201
NM_032967PCDH11X1
NM_024874KIAA0319L1
NM_017861PIGX1
NM_021706LAIR11
NM_002287LAIR11
NM_173079RUNDC11
NM_003167SULT2A11
NM_005440RND21
NM_207333ZNF3201
NM_017856GEMIN81
NM_001042479GEMIN81
NM_001042480GEMIN81
NM_017958PLEKHB21
NM_0011006231
NM_001908CTSB1
NM_147781CTSB1
NM_147783CTSB1
NM_147780CTSB1
NM_147782CTSB1
NM_004744LRAT1
NM_0010988191
NM_0010988181
NM_000923PDE4C1
XM_001128535LOC7288061
NM_015138RTF11
XM_943043LOC6539041
NM_005431XRCC21
NM_152444ZADH11
NM_017592IXL1
NM_001024947YIPF51
NM_030799YIPF51
NM_018706DHTKD11
NM_018243SEPT111
NM_032160DSEL1
NM_213566DFFA1
NM_152913TMEM1301
NM_030626LRRC271
NM_005516HLA-E1
NM_006873STON11
NM_018128TSR11
XM_938924LOC6440921
XM_001130314LOC6440921
NM_170740ALDH5A11
NM_001080ALDH5A11
NM_006864LILRB31
NM_001081450LILRB31
NM_031886KCNA71
NM_052928SMYD41
NM_007047BTN3A21
XM_001134208LOC7299751
NM_004901ENTPD41
NM_133444ZNF5261
NM_001081461JMJD61
NM_052941GBP41
XM_001126406LOC7279221
XM_001126391LOC7279221
XM_001126417LOC7279221
NM_054016FUSIP11
NM_203422LOC2210911
NM_001042508LOC6534831
NM_016183MRTO41
NM_133456SHROOM11
NM_139276STAT31
NM_003150STAT31
NM_213662STAT31
NM_033109PNPT11
NM_012425RSU11
NM_152724RSU11
NM_001083625ANKRD121
NM_015208ANKRD121
NM_133478SLC4A51
NM_004212SLC28A21
NM_000890KCNJ51
NM_001039717C4orf291
XM_929378LOC6464501
NM_022098XPNPEP31
NM_145167PIGM1
NM_001632ALPP1
NM_001992F2R1
NM_020717SHROOM41
NM_177968PPM1B1
NM_001039547GK51
NM_133647SLC12A61
NM_001042495SLC12A61
NM_001042494SLC12A61
NM_001042497SLC12A61
NM_005135SLC12A61
NM_001042496SLC12A61
NM_001077196PDE11A1
NM_001077197PDE11A1
NM_016953PDE11A1
NM_006578GNB51
NM_016194GNB51
NM_006500MCAM1
NM_0011003891
NM_005707PDCD71
XM_001133565LOC7314401
NM_024607PPP1R3B1
NM_012214MGAT4A1
NM_145699APOBEC3A1
NM_018438FBXO61
XM_925853LOC6530531
NM_001037278GGPS11
NM_004837GGPS11
NM_001037277GGPS11
XM_375929LOC4008561
NM_032818C9orf1001
XM_379378LOC4012201
XM_934287LOC1448171
XM_945225LOC1448171
NM_032779FLJ143971
NM_0010996941
NM_024733ZNF6651
NM_144618MGC298911
NM_015719COL5A31
NM_000322PRPH21
NM_002521NPPB1
NM_153675FOXA21
NM_021784FOXA21
NM_004775B4GALT61
NM_000843GRM61
NM_000173GP1BA1
NM_004818DDX231
NM_006038SPATA21
NM_016564CEND11
NM_194326RPS19BP11
NM_032831TMEM142B1
NM_020714ZNF4901
NM_014213HOXD91
NM_016538SIRT71
NM_018210FLJ107691
NM_025081KIAA13051
NM_016929CLIC51
NM_033331CDC14B1
NM_003671CDC14B1
NM_001077181CDC14B1
NM_018327SPTLC31
NM_007033RER11
NM_005124NUP1531
NM_133448TMEM132D1
NM_001002258ATP5G31
NM_001689ATP5G31
NM_020119ZC3HAV11
NM_002081GPC11
NM_032599NYD-SP181
NM_203316DPAGT11
NM_001382DPAGT11
NM_201532DGKZ1
NM_003646DGKZ1
NM_0011055401
NM_201533DGKZ1
NM_0207251
NM_198571FLJ392371
NM_017860C1orf561
NM_194434VAPA1
NM_003574VAPA1
NM_198490RAB431
NM_152612CCDC1161
NM_152253CHKB1
NM_002111HD1
NM_198977ARHGEF11
NM_004706ARHGEF11
NM_199002ARHGEF11
NM_198389PDPN1
NM_006474PDPN1
NM_001006624PDPN1
NM_001006625PDPN1
NM_024327ZNF5761
NM_000599IGFBP51
NM_003128SPTBN11
NM_001669ARSD1
NM_018941CLN81
XM_001127121LOC7282981
NM_144499GNAT11
NM_001040647RCP91
NM_001040648RCP91
NM_014478RCP91
NM_005417SRC1
NM_198291SRC1
NM_020705TBC1D241
NM_014460CSDC21
NM_007353GNA121
NM_020385REXO41
NM_176792MRPL431
NM_024626VTCN11
NM_001003940BMF1
NM_001003943BMF1
NM_001003942BMF1
NM_033503BMF1
NM_138396MARCH91
NM_004670PAPSS21
NM_001015880PAPSS21
NM_183236RAB27A1
NM_004580RAB27A1
NM_183234RAB27A1
NM_183235RAB27A1
NM_002429MMP191
NM_000748CHRNB21
XM_944169LOC3480211
XM_933050LOC3480211
NM_004434EML11
NM_001008707EML11
NM_0208641
NM_022350LRAP1
NM_015056RRP1B1
NM_015456COBRA11
XM_001133009COBRA11
NM_002602PDE6G1
NM_001040032C8orf741
XM_938268TBX181
NM_001080508TBX181
XM_001129120LOC7290341
XM_001133170LOC7288241
NM_017891C1orf1591
NM_000527LDLR1
NM_174976ZDHHC221
NM_005482PIGK1
XM_930505LOC6456761
XM_941520LOC6456761
NM_031904FKSG441
NM_014470RND11
NM_000154GALK11
NM_020947KIAA16091
NM_004711SYNGR11
NM_016089ZNF5891
NM_005160ADRBK21
NM_001375DNASE21
NM_153608ZNF1141
XM_001130495LOC7284291
NM_000842GRM51
NM_020894KIAA15301
NM_207497FLJ437521
NM_000202IDS1
NM_005858AKAP81
NM_001453FOXC11
NM_015326SRGAP21
NM_002319LRCH41
NM_001080115LDB31
NM_001080116LDB31
NM_181704BAGE41
NM_001187BAGE1
NM_182484BAGE51
NM_018226RNPEPL11
NM_015553PIP3-E1
NM_018961UBASH3A1
NM_001001895UBASH3A1
NM_003225TFF11
NM_006595API51
NM_015282CLASP11
NM_080605B3GALT61
NM_004768SFRS111
NM_001271CHD21
NM_032296FLYWCH11
NM_182795NPM21
NM_001478B4GALNT11
NM_139279MCFD21
NM_005920MEF2D1
NM_017724LRRFIP21
NM_001543NDST11
NM_182831TNT1
NM_002862PYGB1
NM_212535PRKCB11
NM_014717ZNF5361
NM_014631SH3PXD2A1
NM_020408LYRM41
NM_002223ITPR21
NM_032109OTP1
NM_001012508SPRN1
NM_005036PPARA1
NM_001001928PPARA1
NM_183419RNF191
NM_015435RNF191
NM_0010400611
NM_145012CCNY1
XM_001134313LOC7322911
XM_001133830LOC7295191
NM_001002762DNAJB121
NM_014517UBP11
NM_000913OPRL11
NM_182647OPRL11
NM_012305AP2A21
NM_018119POLR3E1
NM_003192TBCC1
NM_053276VIT1
NM_032153ZIC41
NM_024563C5orf231
NM_001005209MGC998131
NM_057170GIT21
NM_057169GIT21
NM_014776GIT21
NM_182943PLOD21
NM_000935PLOD21
NM_152643KNDC11
NM_017442TLR91
NM_139170C16orf711
NM_178129P2RY81
NM_080473GATA51
NM_005688ABCC51
NM_012461TINF21
NM_002666PLIN1
NM_024034GDAP1L11
NM_016099GOLGA71
NM_001002296GOLGA71
NM_002608PDGFB1
NM_033016PDGFB1
NM_014665LRRC141
NM_004285H6PD1
NM_152596EXDL11
NM_138572TBN1
NM_001042383CEP631
NM_001042400CEP631
NM_025180CEP631
NM_024667VPS37B1
NM_148920PIGQ1
NM_004204PIGQ1
NM_000761CYP1A21
XM_926474LOC6426481
NM_0011110401
NM_006546IGF2BP11
XM_001134358LOC7322731
NM_007188ABCB81
NM_001039570KREMEN11
NM_001039571KREMEN11
NM_145166ZBTB471
NM_001077503TAC41
NM_001077505TAC41
NM_170685TAC41
NM_001077506TAC41
NM_001077504TAC41
NM_032853MUM11
NM_016210C3orf181
NM_015488PNKD1
NM_022572PNKD1
NM_148962OXER11
XM_001129158LIMS11
NM_004798KIF3B1
NM_021218C9orf801
NM_017651AHI11
NM_002535OAS21
NM_172107KCNQ21
NM_172106KCNQ21
NM_172108KCNQ21
NM_004518KCNQ21
NM_078476BTN2A11
NM_138287DTX3L1
NM_006861RAB351
NM_004953EIF4G11
NM_198244EIF4G11
NM_198241EIF4G11
NM_198242EIF4G11
NM_182917EIF4G11
NM_003751EIF3S91
NM_001037283EIF3S91
NM_020433JPH21
NM_015198COBL1
XM_001134168LOC7299101
XM_001126183LOC7304601
NM_182920ADAMTS91
NM_024843CYBRD11
NM_020746VISA1
NM_001196BID1
NM_197967BID1
NM_197966BID1
NM_001706BCL61
NM_138931BCL61
NM_175062RASGEF1C1
NM_203400RPRML1
XM_943032LOC6478051
NM_006440TXNRD21
NM_015689DENND2A1
NM_079837BANP1
NM_017869BANP1
NM_020448NPAL31
NM_001347DGKQ1
NM_001077621VPS37D1
NM_014062NOB11
NM_177999ASB61
NM_147192DMBX11
NM_172225DMBX11
NM_032902PPP1R16A1
NM_005957MTHFR1
NM_007368RASA31
NM_033381COL4A51
NM_033380COL4A51
NM_000495COL4A51
NM_016500CXorf261
NM_012256ZNF2121
NM_024787RNF1221
NM_014910ZNF5071
NM_033387FAM78A1
NM_018200HMG20A1
NM_006892DNMT3B1
NM_175848DNMT3B1
NM_175849DNMT3B1
NM_175850DNMT3B1
NM_001040273TYSND11
NM_0010992721
NM_152733BTBD91
NM_0528931
NM_173588IGSF221
NM_006569CGREF11
NM_022719DGCR141
NM_001037666LOC6529681
NM_017526LEPROT1
NM_018697LANCL21
NM_030968C1QTNF11
NM_198594C1QTNF11
NM_198593C1QTNF11
NM_014353RAB261
NM_033429CALML41
NM_001031733CALML41
NM_020552TCL61
NM_020553TCL61
NM_024591CHMP61
XM_001132548CHMP61
NM_0010853541
NM_017668NDE11
NM_177539TAS1R11
NM_002401MAP3K31
NM_203351MAP3K31
NM_006646WASF31
NM_000336SCNN1B1
NM_139155ADAMTS141
NM_080722ADAMTS141
NM_022462HIF3A1
NM_152794HIF3A1
NM_152795HIF3A1
NM_018231FLJ108151
NM_020384CLDN21
NM_004171SLC1A21
NM_003615SLC4A71
XM_001125678LOC7304341
XM_001125691LOC7277681
NM_001033515LOC3898331
XM_001125765LOC7277641
XM_001125832LOC7277641
NM_144705TEKT41
XM_001134008KRTAP10-21
XM_001134010KRTAP10-21
NM_198693KRTAP10-21
NM_198690KRTAP10-91
NM_006137CD71
NM_0212411
NM_006454MXD41
NM_001013622FAM53A1
NM_003684MKNK11
NM_144664FAM76B1
NM_015064ERC11
NM_178039ERC11
NM_178040ERC11
NM_178038ERC11
NM_178037ERC11
NM_000303PMM21
NM_152381CMYA31
NM_004061CDH121
XM_001127975FAM66C1
NM_004491GRLF11
NM_014690FAM131B1
NM_001031690FAM131B1
NM_018651ZNF1671
NM_181523PIK3R11
NM_181504PIK3R11
NM_181524PIK3R11
XM_495939KIAA15451
XM_936428KIAA15451
NM_152269C12orf651
NM_0011031611
NM_0011031601
NM_176072P2RY21
NM_002564P2RY21
NM_176071P2RY21
NM_174937TCERG1L1
NM_181458PAX31
NM_181460PAX31
NM_181457PAX31
NM_181459PAX31
NM_022153C10orf541
NM_148894FAM44A1
NM_001029871RSPO41
NM_001040007RSPO41
NM_024630ZDHHC141
NM_153746ZDHHC141
XM_001131408LOC7303501
XM_001127666LOC7308961
NM_001004720NCK21
NM_001004722NCK21
NM_003581NCK21
NM_025219DNAJC51
NM_178865SERINC21
NM_001039211ATAD3C1
NM_032792ZBTB451
NM_1449931
NM_030766BCL2L141
NM_001040628NCALD1
NM_001040626NCALD1
NM_001040625NCALD1
NM_032041NCALD1
NM_001040629NCALD1
NM_001040624NCALD1
NM_001040627NCALD1
NM_001040630NCALD1
NM_001079691CG0181
NM_173344ST3GAL11
NM_003033ST3GAL11
NM_020822KCNT11
NM_001039397FLJ402441
NM_004348RUNX21
NM_001015051RUNX21
NM_001024630RUNX21
NM_002417MKI671
NM_002350LYN1
NM_0011110971
NM_002035FVT11
NM_018075TMEM16K1
NM_001440EXTL31
NM_004032DDO1
NM_003649DDO1
NM_207335KLHDC61
NM_021012KCNJ121
NM_001017924LRRC221
NM_021962ABR1
NM_001092ABR1
NM_183078RNF81
NM_003958RNF81
NM_000875IGF1R1
NM_005188CBL1
NM_175852TXLNA1
NM_153038FLJ324471
NM_178554KY1
XM_930755LOC6530561
XM_001129100LOC7290281
XM_001131704LOC7299001
XM_001127145LOC7283101
NM_0010096101
NM_015075IQSEC21
NM_001467SLC37A41
NM_015329KIAA08921
XM_930431LOC6451951
XM_942574LOC6451951
NM_005432XRCC31
NM_0011001191
NM_0011001181
NM_001010846SHE1
NM_018302C4orf191
NM_0011046291
NM_014864FAM20B1
NM_001238CCNE11
NM_057182CCNE11
NM_005735ACTR1B1
NM_018225SMU11
XM_001130919LOC7296521
NM_024512LRRC21
NM_025136OPA31
NM_015533DAK1
NM_002203ITGA21
XM_001129103LOC7290291
XM_001128912LOC7312381
NM_032932RAB11FIP41
NM_018396METTL2B1
NM_019079L1TD11
NM_032374C14orf1531
NM_000629IFNAR11
NM_175066DDX511
NM_000745CHRNA51
NM_173518C8orf451
NM_032826SLC35B41
NM_144682SLFN131
NM_170710WDR171
NM_181265WDR171
NM_024596MCPH11
NM_024881SLC35E11
NM_004332BPHL1
NM_145212MRPL301
NM_014285EXOSC21
XM_941062FLJ418561
XM_371176FLJ418561
NM_005793NME61
NM_001033055AIPL11
NM_001033054AIPL11
NM_014336AIPL11
NM_032177RNUXA1
NM_148903GREB11
NM_014339IL17RA1
NM_006455SC651
NM_020340KIAA12441
NM_0208551
NM_153603COG71
NM_197964HSPC2681
NM_018656SLC35E31
NM_133646ZAK1
NM_031439SOX71
NM_002098GUCA1B1
NM_007163SLC14A21
XM_001132858LOC7314241
NM_152263TPM31
NM_001030060SAMD51
NM_018097CEP271
NM_014293NPTXR1
NM_0011031491
NM_018215FLJ107811
NM_182612PDDC11
NM_005151USP141
NM_001037334USP141
NM_032811TBRG11
NM_021635PBOV11
NM_138400NOM11
NM_144615TMIGD21
NM_017563IL17RD1
NM_001080973IL17RD1
XM_001129151LOC7290471
NM_139008HFE1
NM_139003HFE1
NM_139010HFE1
NM_139011HFE1
NM_139009HFE1
NM_000410HFE1
NM_139004HFE1
NM_139007HFE1
NM_139006HFE1
NM_020208SLC6A201
NM_022405SLC6A201
NM_001081637LILRB11
NM_006669LILRB11
NM_001081639LILRB11
NM_001081638LILRB11
NM_148960CLDN191
NM_182594ZNF4541
NM_138458LOC1161431
NM_018379FAM63A1
NM_001040217FAM63A1
NM_024026MRP631
NM_000417IL2RA1
NM_198076FAM36A1
NM_001033578SGK31
NM_013257SGK31
NM_170709SGK31
NM_147134NFX11
NM_032433ZNF3331
NM_005027PIK3R21
NM_152487TMEM561
NM_005216DDOST1
NM_006134TMEM50B1
NM_145341PDCD41
NM_014456PDCD41
NM_182578THEM51
NM_014331SLC7A111
NM_024318LILRA61
NM_001031672LOC6064951
NM_001033855DCLRE1C1
NM_001033857DCLRE1C1
NM_001033858DCLRE1C1
NM_022487DCLRE1C1
NM_007210GALNT61
NM_006903PPA21
NM_176866PPA21
NM_176869PPA21
NM_176867PPA21
NM_001034191PPA21
NM_015666GTPBP51
NM_012434SLC17A51
NM_024311MFSD111
NM_014772KIAA04271
NM_001039029LRTM21
NM_015246MGRN11
NM_002645PIK3C2A1
NM_153451ORAOV11
NM_001081491NXF11
NM_017515SLC35F21
NM_020394ZNF6951
NM_0010981991
NM_005279GPR11
NM_003636KCNAB21
NM_172130KCNAB21
NM_182705FAM101B1
XM_001126665FAM101B1
NM_080657RSAD21
NM_199072MDFIC1
NM_015151DIP2A1
XM_209363SIRPB21
XM_939897SIRPB21
NM_183397PXMP41
NM_007238PXMP41
NM_012405ICMT1
NM_016641MIR161
NM_019116UBFD11
NM_032489ACRBP1
NM_022479WBSCR171
NM_004416DTX11
NM_153442GPR261
NM_022046KLK141
NM_170721MSI21
NM_0011107811
NM_138465GLI41
NM_207514FLJ201861
NM_001080395AATK1
NM_001004019FBLN21
NM_001998FBLN21
NM_138793CANT11
XM_001130949LOC1583761
XM_001129749LOC1583761
NM_000445PLEC11
NM_201379PLEC11
NM_201382PLEC11
NM_201383PLEC11
NM_201380PLEC11
NM_201381PLEC11
NM_201384PLEC11
NM_201378PLEC11
NM_004481GALNT21
NM_152547BTNL91
NM_054035UNC1191
NM_005148UNC1191
NM_175629DNMT3A1
NM_022552DNMT3A1
NM_153759DNMT3A1
NM_001420ELAVL31
NM_032281ELAVL31
NM_001010926HES51
NM_018238AGK1
NM_014855KIAA04151
NM_015576ERC21
NM_182724CREM1
NM_182772CREM1
NM_181571CREM1
NM_182853CREM1
NM_182718CREM1
NM_182771CREM1
NM_182722CREM1
NM_183060CREM1
NM_182717CREM1
NM_182770CREM1
NM_182721CREM1
NM_183012CREM1
NM_182719CREM1
NM_182725CREM1
NM_183011CREM1
NM_182850CREM1
NM_182723CREM1
NM_182720CREM1
NM_182769CREM1
NM_183013CREM1
NM_012072CD931
NM_0010994321
NM_017679BCAS31
NM_017945SLC35A51
NM_138450ARL111
NM_182501MTERFD21
NM_145296CADM41
NM_080797DIDO11
NM_015645C1QTNF51
NM_020909EPB41L51
NM_017454STAU11
NM_004602STAU11
NM_017452STAU11
NM_001037328STAU11
NM_017453STAU11
NM_033107GTPBP101
NM_001042717GTPBP101
NM_171982TRIM351
XM_927226LOC6429211
NM_173471SLC25A261
NM_021646ZNF5001
NM_152361EID2B1
NM_014732KIAA05131
NM_174918C19orf591
NM_013336SEC61A11
NM_003239TGFB31
NM_005663WHSC21
NM_000168GLI31
NM_015079TBC1D2B1
NM_020632ATP6V0A41
NM_130840ATP6V0A41
NM_130841ATP6V0A41
NM_0010997891
NM_000873ICAM21
NM_0010997871
NM_0010997861
NM_0010997881
NM_018049PLEKHJ11
NM_144769FOXI11
NM_012188FOXI11
NM_001001894TTC31
NM_003316TTC31
NM_006890CEACAM71
NM_138499PWWP21
NM_0010986371
NM_207406CCDC41
NM_006944SPP21
NM_017411SMN21
NM_022875SMN21
NM_022877SMN21
NM_022876SMN21
NM_000344SMN11
NM_022874SMN11
NM_007237SP1401
NM_000451SHOX1
NM_006926SFTPA21
NM_0010986681
NM_024738C12orf491
NM_032012C9orf51
NM_0010997341
NM_025230WDR231
NM_181357WDR231
NM_003196TCEA31
NM_002293LAMC11
NM_018660ZNF3951
NM_173354SNF1LK1
NM_001020658PUM11
NM_014676PUM11
NM_000182HADHA1
NM_207446LOC4004511
NM_0011003901
NM_152558IQCE1
NM_006548IGF2BP21
NM_001007225IGF2BP21
NM_005748YAF21
NM_033337CAV31
NM_001234CAV31
NM_015225KIAA03671
NM_013322SNX101
NM_015455CNOT61
NM_021813BACH21
XM_001130573LOC7317271
XM_001130089LOC7293761
XM_001131004LOC7318401
XM_001131944LOC7299801
NM_015332NUDCD31
NM_018708FEM1A1
NM_013276CARKL1
NM_001042573FLJ218651
XM_934624LOC6472641
XM_001133898LOC7319321
NM_014974DIP2C1
NM_003881WISP21
NM_020836KIAA14461
NM_024735FBXO311
NM_006682FGL21
NM_198576AGRN1
XM_001126326AGRIN1
NM_024866ADM21
XM_001129415FLJ226751
XM_001129697FLJ226751
NM_002861PCYT21
NM_001031738TMEM1501
NM_153342TMEM1501
XM_001132907LOC7302021
XM_001131136LOC7318871
NM_017670OTUB11
NM_001039762FLJ455571
XM_926071LOC6425921
NM_016337EVL1
XM_378914KIAA04921
XM_944290KIAA04921
NM_000049ASPA1
NM_022749RAI161
NM_000112SLC26A21
NM_017831RNF1251
NM_153611CYBASC31
XM_937698LOC6486291
NM_020895GRAMD1A1
NM_005937MLLT61
NM_0010994131
NM_0010994121
NM_006766MYST31
NM_016520C9orf781
NM_004809STOML11
NM_014686KIAA03551
NM_030819GFOD21
NM_001012761RGMB1
NM_002230JUP1
NM_003800RNGTT1
NM_002732PRKACG1
NM_001042427C10orf531
NM_006946SPTBN21
NM_001001549GRB101
NM_001001555GRB101
NM_001001550GRB101
NM_005311GRB101
NM_006058TNIP11
NM_174917LOC1973221
NM_021248CDH221
XM_001131068LOC7296961
XM_001129643LOC7314351
NM_015090NFASC1
NM_001670ARVCF1
NM_019892INPP5E1
NM_173515CNKSR31
NM_001083591CUGBP21
NM_001025077CUGBP21
NM_006561CUGBP21
NM_001025076CUGBP21
NM_025224ZBTB461
NM_006739MCM51
NM_197949CLEC7A1
NM_197950CLEC7A1
NM_022570CLEC7A1
NM_197948CLEC7A1
NM_197947CLEC7A1
NM_006888CALM11
NM_020177FEM1C1
NM_153023SPATA131
NM_001083909GPR1231
NM_033449FCHSD11
NM_016479SCOTIN1
NM_022648TNS11
NM_201520SLC25A351
NM_003201TFAM1
NM_001040649ACP11
NM_001360DHCR71
NM_139323YWHAB1
NM_003404YWHAB1
NM_015137KIAA01431
NM_145263SPATA181
NM_145244DDIT4L1
NM_015044GGA21
NM_004571PKNOX11
NM_002957RXRA1
NM_001039591USP9X1
NM_001039590USP9X1
NM_138468ICA1L1
NM_004319ASTN11
NM_016143NSFL1C1
NM_182483NSFL1C1
NM_018839NSFL1C1
NM_024086METT10D1
NM_007369GPR1611

Immunostimulatory motif search results


UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).

GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).



Settings used for search

Scores were calculated using scoring scheme SS1
Using AVG scoring mode.

Hit scores

ParameterSpec. Score cut-offColourComment
Hit to same geneColourHits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit.
High scoring hit≥80ColourHits are very likely to cause an off-target effect
Medium scoring hit<80¸≥40ColourHits with a small chance of causing an off target effect.
Low scoring hitColourHits very unlikely to cause an off-target effect

The mismatches are colour coded based on the weighting of that position.

Cut-offColour
MatchColour
Wobble base pairs (G-U)Colour
Critical bases (none)Colour
0.6 bitsColour
0.4 bitsColour
0.2 bitsColour

About SpecificityServer

SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).

Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer


For more details about the algorithm "siRNA specificity searching incorporating mismatch tolerance data" Bioinformatics (2008) 24 (10): 1316-1317