Predicted Off-targets for siRNA "cccuacaccaagugauaau" Using siRNA SpecificityServer

Specificity Server [Result page v1.0]


SpecificityServer - An siRNA design program for determining siRNA specificity

Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands

10 hits were found for CCCUACACCAAGUGAUAAU (8 guide strand hits and 2 sense strand hits.)

Hits to Guide strand

AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
NM_017988 SCYL2 2324 2342 uuCUACACCAAcUGAUAAU52 3 9   false   <90%   3818
NM_000138 FBN1 9647 9665 guaUACACCAAGUGgUAAU42 4 11 c false   <90%   11626
NM_022765 MICAL1 2622 2640 CCCcACACCAAGUGAgAAU37 2 11 c false   <90%   3697
NM_172193 KLHDC1 388 406 aCCUACACCAcGUGAUAAa31 3 9 c false   <90%   2688
NM_145048 C4orf28 303 321 uCCUAgACCAAGUGAUAAa29 3 12 bc false   <90%   1825
NM_031279 AGXT2L1 1799 1817 gaaUAaACCAAGUGAUAAU3 4 13 bc false   <90%   2107
NM_013348 KCNJ14 2980 2998 CCCUACACCuAGUGAUgug-13 4 9 c false   <90%   3049
NM_170720 KCNJ14 3051 3069 CCCUACACCuAGUGAUgug-13 4 9 c false   <90%   3120


Hits to Sense strand



AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
NM_001663 ARF6 1467 1485 cUUAUCACUUGcUGUAGau56 4 10   false   <90%   1806
NM_153015 TMEM74 2020 2038 ugUAUCACUUGcUGUAGGc47 4 9   false   <90%   2087


3 prime UTR Seed matches

Hits to same gene : 0
Hits to other genes : 1235
View matched accessions

3 prime seed match details

AccessionGene nameHits to this gene
NM_175910ZNF4935
NM_001076678ZNF4935
NM_015852ZNF1175
NM_002251KCNS14
NM_006524ZNF1384
NM_007153ZNF2083
NM_001001411ZNF6763
NM_032569N-PAC3
NM_001076675ZNF6263
NM_172069PLEKHH23
NM_001080412ZBTB383
XM_001133510ZBTB383
NM_031309SCRT13
NM_001918DBT3
NM_001010851ZNF7662
NM_001012339DNAJA52
NM_194283DNAJA52
NM_004286GTPBP12
NM_003430ZNF912
NM_006148LASP12
NM_014322OPN32
NM_021047ZNF2532
NM_0010992692
NM_020947KIAA16092
NM_016220ZNF5882
NM_001013746ZNF5882
NM_020394ZNF6952
NM_033468ZNF2572
NM_001001396ATP2B42
XM_001130526LOC7286362
XM_001130555LOC7286362
XM_001130543LOC7286362
XM_001130536LOC7286362
XM_925864LOC6423252
XM_001129399LOC6423462
XM_928707LOC6423462
XM_925879LOC6423462
NM_0121942
NM_004079CTSS2
NM_001031804MAF2
NM_001004439ITGA112
NM_000719CACNA1C2
NM_000578SLC11A12
NM_206925CA122
NM_001218CA122
NM_022169ABCG42
NM_002657PLAGL22
NM_003441ZNF1412
NM_000068CACNA1A2
NM_178013PRIMA12
NM_0011097542
NM_002837PTPRB2
NM_014770CENTG12
NM_000554CRX2
NM_005538INHBC2
NM_004028AQP42
NM_001650AQP42
NM_001939DRP22
NM_020731AHRR2
XM_209227LOC6534642
NM_033397KIAA17542
NM_002460IRF42
NM_0011055792
NM_001457FLNB2
NM_001030060SAMD52
NM_021137TNFAIP12
NM_005063SCD2
NM_004586RPS6KA32
NM_005722ACTR22
NM_001005386ACTR22
NM_0011008292
NM_002762PRM22
NM_177405CECR12
NM_017424CECR12
NM_207387C17orf762
XM_944838LOC2857332
XM_379432LOC2857332
NM_005739RASGRP11
NM_022148CRLF21
XM_291020LOC3398091
XM_936940LOC3398091
NM_015335THRAP21
NM_016426GTSE11
NM_000417IL2RA1
NM_016206VGLL31
NM_173582PGM2L11
NM_005852CHD31
NM_001005271CHD31
NM_001005273CHD31
NM_003855IL18R11
NM_012316KPNA61
NM_003099SNX11
NM_152826SNX11
NM_148955SNX11
NM_004098EMX21
NM_002033FUT41
NM_001892CSNK1A11
NM_001025105CSNK1A11
NM_002351SH2D1A1
NM_001043352TPM31
NM_001043353TPM31
NM_016132MYEF21
NM_003622PPFIBP11
NM_025195TRIB11
NM_017952PTCD31
NM_020830WDFY11
NM_016255FAM8A11
NM_000493COL10A11
NM_001031680RUNX31
NM_004350RUNX31
NM_020774MIB11
NM_005826HNRPR1
NM_0011023981
NM_0011023971
NM_0011023991
NM_145341PDCD41
NM_014456PDCD41
NM_0011051931
NM_0011051951
NM_0011051941
NM_152698FLJ383771
NM_138572TBN1
NM_173629C18orf261
NM_152227SNX51
NM_014426SNX51
NM_032566SPINK71
NM_024843CYBRD11
NM_176894P2RY131
NM_023914P2RY131
NM_024323C19orf571
NM_001941DSC31
NM_024423DSC31
NM_033554HLA-DPA11
NM_007198PROSC1
NM_032423ZNF5281
NM_178544ZNF5461
NM_018411HR1
NM_005144HR1
NM_003217TEGT1
NM_0010985761
NM_001001346CLDN201
NM_004464FGF51
NM_033143FGF51
NM_005551KLK21
NM_001002231KLK21
NM_001310CREBL21
NM_002941ROBO11
NM_133631ROBO11
NM_020133AGPAT41
NM_014702KIAA04081
NM_017415KLHL31
NM_020692GALNTL11
NM_017824MARCH51
NM_020297ABCC91
NM_001011720XKR91
NM_001011552SLC9A41
NM_002547OPHN11
NM_001008407BCAP291
NM_183237RNF71
NM_014245RNF71
NM_001039577CCNL21
NM_006321ARIH21
NM_003821RIPK21
NM_017506OR7A51
NM_017902HIF1AN1
NM_153611CYBASC31
NM_014653WSCD21
NM_021269ZNF7081
NM_003423ZNF431
NM_0011024211
NM_0011024201
NM_006007ZFAND51
XM_001126961LOC7302951
XM_001129007LOC7312651
NM_003414ZNF2671
NM_173578C11orf721
NM_021954GJA31
NM_025160WDR261
NM_032606CAPS21
NM_015886PI151
NM_001079529LOC2021341
NM_0178921
NM_005468NAALADL11
NM_020796SEMA6A1
NM_001014797KCNMA11
NM_001007097NTRK21
NM_000084CLCN51
NM_001029839C3orf231
NM_001029840C3orf231
XM_001129158LIMS11
NM_001039752SLC22A101
NM_144767AKAP131
NM_006738AKAP131
NM_007200AKAP131
NM_021823PPCDC1
NM_175744RHOC1
NM_001042679RHOC1
NM_001042678RHOC1
XM_933066LOC6436961
XM_929030LOC1615271
NM_006150LMO61
NM_017613DONSON1
NM_024674LIN281
NM_153266TMEM1511
XM_001133285TMEM1511
XM_928464LOC1465171
NM_004977KCNC31
NM_018133MSL2L11
NM_152515CKAP2L1
XM_928240LOC3899061
XM_942518LOC3899061
NM_003734AOC31
NM_014408TRAPPC31
NM_144968RIBC11
XM_001133042PDZRN31
NM_015009PDZRN31
NM_014313TMEM50A1
NM_019084CCNJ1
NM_001077349ZNF6821
NM_033196ZNF6821
NM_014948UBOX51
NM_199415UBOX51
NM_015176FBXO281
NM_152551C6orf1511
NM_001079653SDK11
NM_152744SDK11
NM_145268C7orf451
NM_024596MCPH11
NM_012092ICOS1
NM_1814261
NM_006182DDR21
NM_001014796DDR21
NM_032503MCHR21
NM_001040179MCHR21
NM_005933MLL1
NM_014048MKL21
NM_000668ADH1B1
NM_138972BACE11
NM_012104BACE11
NM_138973BACE11
NM_138971BACE11
NM_002463MX21
NM_153487MDGA11
NM_014474SMPDL3B1
NM_000216KAL11
NM_198510ITIH5L1
NM_003170SUPT6H1
NM_201266NRP21
NM_003872NRP21
NM_201279NRP21
NM_001077196PDE11A1
NM_001077197PDE11A1
NM_016953PDE11A1
NM_017772TBC1D22B1
NM_001012509SLC45A21
NM_173622CDRT41
NM_003644GAS71
NM_201432GAS71
NM_201433GAS71
NM_213606SLC16A121
NM_182924MICALL21
NM_080491GAB21
NM_012296GAB21
NM_017644KLHL241
NM_000481AMT1
NM_015474SAMHD11
NM_001858COL19A11
NM_001387DPYSL31
NM_018225SMU11
NM_015995KLF131
NM_020462ERGIC11
NM_052907TMEM132B1
NM_018706DHTKD11
NM_001010922C1orf1781
NM_013443ST6GALNAC61
NM_006969ZNF281
NM_153234LIX11
NM_001933DLST1
NM_020777SORCS21
NM_024584CCDC1211
NM_006037HDAC41
NM_018313PBRM11
NM_181042PBRM11
NM_018165PBRM11
NM_001005463EBF31
XM_928549LOC6455291
NM_177965C8orf371
NM_019096GTPBP21
NM_144982CCDC1311
NM_002025AFF21
NM_001039396MPEG11
NM_014759PHYHIP1
NM_0010993351
NM_002396ME21
NM_198484ZNF6211
NM_0010984141
NM_033276XRCC6BP11
NM_052897MBD61
NM_003458BSN1
NM_145207SPATA51
NM_016089ZNF5891
NM_001045477FAM22G1
NM_0010096101
NM_020443NAV11
NM_031490LONP21
XM_936279LOC6421321
NM_020367PARP111
NM_001003702FLJ436921
NM_153453VGLL21
NM_182645VGLL21
NM_024779PIP5K2C1
NM_000478ALPL1
NM_0010997331
NM_001117ADCYAP11
XM_001130518LOC7284851
XM_001133894LOC7319641
NM_015286DMN1
NM_145728DMN1
NM_004443EPHB31
NM_016395PTPLAD11
XM_001133298LOC7324021
NM_001001389CD441
NM_001001390CD441
NM_001001391CD441
NM_001001392CD441
NM_000610CD441
NM_004567PFKFB41
NM_020871LRCH21
XM_001130851LOC7317991
XM_001130715LOC7295891
NM_025218ULBP11
NM_024887DHDDS1
NM_205861DHDDS1
NM_017420SIX41
NM_000052ATP7A1
NM_024704C20orf231
NM_006281STK31
NM_012234RYBP1
NM_018963BRWD11
NM_004979KCND11
NM_015113ZZEF11
NM_182515ZNF7141
NM_001079669TMTC41
NM_032813TMTC41
NM_006812OS91
NM_001017958OS91
NM_001017957OS91
NM_001017956OS91
NM_152292RG9MTD21
NM_001009959KIAA11891
NM_020711KIAA11891
NM_171998RAB39B1
NM_002737PRKCA1
NM_198150ARSK1
NM_004090DUSP31
NM_199418PRCP1
NM_005040PRCP1
NM_003200TCF31
NM_006011ST8SIA21
XM_001133559LOC6446341
NM_002508NID11
NM_001010853ACY1L21
NM_144576COQ10A1
NM_0010993371
NM_182935MOBP1
NM_024807TREML21
NM_013238DNAJC151
NM_014228SLC6A71
NM_001005505CACNA2D21
NM_006030CACNA2D21
NM_199334THRA1
NM_020863ZFAT11
NM_005029PITX31
NM_001018100Gcom11
NM_152451Gcom11
NM_020431TMEM63C1
NM_000918P4HB1
NM_001610ACP21
NM_030981RAB1B1
NM_020375C12orf51
NM_002430MN11
NM_022461AZI21
NM_018201TBC1D131
NM_016535ZNF5811
NM_144719CCDC131
NM_021924MUPCDH1
NM_031264MUPCDH1
NM_138460CMTM51
NM_001037288CMTM51
XM_936831IQSEC31
NM_015321CRTC11
NM_0010984821
NM_014696GPRIN21
NM_021083XK1
NM_001421ELF41
NM_024946NIP301
NM_198098AQP11
NM_024040CUEDC21
NM_182757IBRDC21
NM_153711FAM26E1
NM_006772SYNGAP11
NM_002161IARS1
NM_013417IARS1
NM_175053KRT741
NM_001386DPYSL21
NM_003227TFR21
NM_178173CCDC361
NM_004776B4GALT51
NM_002242KCNJ131
NM_012219MRAS1
NM_0010850491
NM_015275KIAA10331
NM_015282CLASP11
XM_939547ZNF6971
NM_001080470ZNF6971
NM_004780TCEAL11
NM_001006640TCEAL11
NM_001006639TCEAL11
NM_138773SLC25A461
XM_944198LOC4002361
XM_378472LOC4002361
NM_001878CRABP21
NM_007137ZNF811
NM_002064GLRX1
NM_014398LAMP31
NM_017944USP471
NM_017875SLC25A381
NM_007021C10orf101
NM_032827ATOH81
NM_000066C8B1
NM_031890CECR61
NM_002981CCL11
NM_006020ALKBH11
NM_019067GNL3L1
NM_023035CACNA1A1
XM_001133209LOC6465131
XM_001132717LOC6465131
NM_002235KCNA61
NM_004355CD741
NM_001025159CD741
NM_001025158CD741
NM_032572RNASE71
NM_018667SMPD31
NM_014337PPIL21
NM_015471NSL11
NM_001042549NSL11
NM_002535OAS21
NM_022910NDRG41
NM_020465NDRG41
NM_001039591USP9X1
NM_001039590USP9X1
NM_003799RNMT1
NM_001029882AHDC11
NM_005505SCARB11
NM_002188IL131
NM_032325MGC111021
NM_152571C9orf1631
NM_014810CEP3501
XM_936809LOC3429181
XM_292779LOC3429181
NM_006888CALM11
NM_004427PHC21
NM_198040PHC21
NM_006097MYL91
NM_181526MYL91
NM_019014POLR1B1
NM_016057COPZ11
NM_005439MLF21
NM_152787MAP3K7IP31
NM_000321RB11
NM_138813ATP8B31
NM_013348KCNJ141
NM_170720KCNJ141
NM_016173HEMK11
NM_002074GNB11
NM_001552IGFBP41
NM_000674ADORA11
NM_001048230ADORA11
NM_017460CYP3A41
NM_002404MFAP41
NM_174902LDLRAD31
NM_001037293PALM21
NM_053016PALM21
NM_001077350C16orf351
NM_001039476C16orf351
NM_017586C9orf71
NM_197941ADAMTS61
NM_014286FREQ1
NM_000539RHO1
NM_013336SEC61A11
NM_015365AMMECR11
NM_001025580AMMECR11
NM_001025109CD341
NM_001773CD341
NM_015332NUDCD31
NM_020347LZTFL11
NM_006830UQCR1
NM_002310LIFR1
NM_173075APBB21
NM_014015DEXI1
NM_006237POU4F11
NM_052861MGC216751
NM_015527TBC1D10B1
XM_930224LOC6471901
XM_941815LOC6471901
NM_021191NEUROD41
NM_152916EMR21
NM_152918EMR21
NM_152917EMR21
NM_152920EMR21
NM_152921EMR21
NM_013447EMR21
NM_152919EMR21
NM_177999ASB61
NM_017873ASB61
NM_031442TMEM471
NM_018161NADSYN11
NM_020742NLGN4X1
NM_181332NLGN4X1
NM_001008494ISX1
XM_379228LOC4010931
XM_944985LOC4010931
NM_001012720RGR1
NM_002921RGR1
NM_001012722RGR1
NM_016563RASL121
NM_004256SLC22A131
NM_001018111PODXL1
NM_005397PODXL1
NM_020148SPIRE11
NM_015343DULLARD1
NM_017410HOXC131
NM_001076787TP53I111
NM_006034TP53I111
NM_006426DPYSL41
NM_145230ATP6V0E21
NM_0011005921
NM_020956PRX1
NM_172214CAMKK21
NM_172215CAMKK21
XM_001133170LOC7288241
XM_001129120LOC7290341
NM_000345SNCA1
NM_007308SNCA1
NM_022060ABHD41
NM_203394E2F71
NM_032414PROK11
NM_173598KSR21
NM_021229NTN41
NM_001924GADD45A1
NM_145245EVI5L1
NM_0010087811
XM_936538FAT31
XM_926199FAT31
NM_002855PVRL11
NM_002376MARK31
NM_003882WISP11
NM_014233UBTF1
NM_001076683UBTF1
NM_001076684UBTF1
NM_006203PDE4D1
NM_0011046311
NM_003621PPFIBP21
NM_018178GOLPH3L1
NM_006300ZNF2301
NM_172367TUSC51
NM_001042413GLIS31
NM_152629GLIS31
NM_015042ZNF6091
NM_144974CCDC1221
NM_020129LGALS141
NM_203471LGALS141
NM_147180PPP3R21
NM_021734SLC25A191
XM_086001LOC1480031
XM_942526LOC6511021
NM_015554GLCE1
NM_0010129671
NM_002103GYS11
NM_177538CYP20A11
NM_004730ETF11
NM_013268LGALS131
NM_014325CORO1C1
XM_001125791LOC6427881
XM_001127886LOC7281001
XM_001132421LOC3883121
XM_001127568LOC7284991
XM_001127665LOC7285261
XM_001130826LOC7284521
XM_001127715LOC7285401
XM_001127825LOC7285751
XM_001130837LOC6433671
XM_932646LOC3400891
NM_022753S100PBP1
NM_003157NEK41
NM_014771RNF401
NM_178844NLRC31
NM_000555DCX1
NM_178152DCX1
NM_178153DCX1
NM_178151DCX1
NM_024707GEMIN71
NM_001007270GEMIN71
NM_001007269GEMIN71
NM_175900C16orf541
XM_001128758LOC7280701
NM_0010987211
NM_0010987221
NM_004485GNG41
NM_001008539SLC7A21
NM_003046SLC7A21
NM_198867ALKBH61
NM_001038707CDC42SE11
NM_020239CDC42SE11
NM_0011031471
NM_0011031481
NM_0011031461
NM_015575TNRC151
NM_005819STX61
NM_139246C9orf971
NM_002523NPTX21
NM_006464TGOLN21
NM_001024843TNRC6B1
NM_015088TNRC6B1
NM_030920ANP32E1
NM_021117CRY21
NM_024076KCTD151
NM_017645FAM29A1
NM_001364DLG21
NM_001083621ZBTB401
NM_014870ZBTB401
NM_001963EGF1
NM_001017964LOC1502231
NM_032807FBXO181
NM_178150FBXO181
NM_001005609EDA1
NM_001399EDA1
NM_0010985001
NM_019590KIAA12171
NM_0010985011
NM_003038SLC1A41
NM_000961PTGIS1
NM_020402CHRNA101
NM_021116ADCY11
NM_005624CCL251
NM_002063GLRA21
NM_172071RC3H11
NM_080669SLC46A11
NM_033262SLC8A31
NM_183002SLC8A31
NM_058240SLC8A31
NM_182936SLC8A31
NM_182932SLC8A31
NM_001079877RASA41
NM_006989RASA41
NM_145259ACVR1C1
NM_0011110311
NM_0011110331
NM_0011110321
NM_001466FZD21
NM_000950PRRG11
NM_018409LRP2BP1
NM_138400NOM11
NM_001005377PLAUR1
NM_002659PLAUR1
NM_015155LARP51
NM_021912GABRB31
NM_000814GABRB31
NM_014671UBE3C1
NM_001079512LOC7807761
NM_032520GNPTG1
NM_013305ST8SIA51
NM_015235CSTF2T1
NM_181725METTL2A1
NM_001005372METTL2A1
NM_002747MAPK41
NM_002360MAFK1
NM_018223CHFR1
NM_001259CDK61
NM_001950E2F41
NM_1992871
NM_014405CACNG41
NM_015313ARHGEF121
NM_152673MUC201
NM_0010985161
NM_030969TMEM14B1
NM_033542SYS11
NM_014383ZBTB321
NM_000570FCGR3B1
NM_012308FBXL111
NM_030634ZNF4361
NM_001077195ZNF4361
NM_001012755MCART61
NM_001627ALCAM1
NM_006651CPLX11
NM_031950FGFBP21
NM_019083CCDC761
NM_022772EPS8L21
XM_001129221EPS8L21
NM_203406LOC1533641
NM_022437ABCG81
NM_005437NCOA41
NM_021783EDA2R1
NM_014289CAPN61
NM_0010992701
NM_021645UTP14C1
NM_173084TRIM591
NM_025081KIAA13051
NM_015986CRLF31
NM_175611GRIK11
NM_032974CASP101
NM_173548ZNF5841
NM_004484GPC31
NM_003983SLC7A61
NM_001076785SLC7A61
NM_153051MTMR31
NM_021090MTMR31
NM_153050MTMR31
NM_014839LPPR41
NM_018977NLGN31
NM_018393TCP11L11
NM_015191SNF1LK21
XM_926793LOC6427571
XM_942502LOC6427571
NM_012458TIMM131
NM_002417MKI671
NM_007353GNA121
NM_003664AP3B11
NM_032291SGIP11
XM_001131899LOC2839041
XM_001126290LOC7279081
NM_052924RHPN11
NM_014726TBKBP11
NM_020132AGPAT31
NM_001037553AGPAT31
NM_005906MAK1
NM_005228EGFR1
NM_006682FGL21
NM_0011132391
NM_022740HIPK21
NM_0010996821
NM_024859MAGIX1
NM_0010996801
NM_0010996811
NM_171825CAMK2A1
NM_015981CAMK2A1
NM_015225KIAA03671
NM_016539SIRT61
NM_015219EXOC71
NM_001013839EXOC71
NM_001010866RP13-15M17.21
NM_015345DAAM21
NM_001042595TMEM911
NM_0010988211
NM_001848COL6A11
NM_032269CCDC1351
XM_001133300C16orf501
NM_147202C9orf251
XM_001132988FLJ217671
XM_379820FLJ217671
NM_014858TMCC21
NM_005458GABBR21
NM_014872ZBTB51
NM_181553CMTM31
NM_001048251CMTM31
NM_181554CMTM31
NM_144601CMTM31
NM_020158EXOSC51
NM_007087CALB21
NM_001740CALB21
NM_007088CALB21
NM_015107PHF81
NM_015488PNKD1
NM_022572PNKD1
NM_017523XAF11
NM_199139XAF11
NM_014752SPCS21
NM_014965TRAK11
NM_146388MRPL41
NM_1383821
NM_001004316FLJ166411
NM_006974ZNF33A1
NM_006954ZNF33A1
NM_0010996921
NM_025236RNF391
NM_170769RNF391
NM_020211RGMA1
NM_025240CD2761
NM_001024736CD2761
NM_005935AFF11
NM_006059LAMC31
XM_929034LOC6460741
NM_181656C17orf581
NM_001007088ZNF1821
NM_006962ZNF1821
NM_014246CELSR11
NM_016946F11R1
NM_018999KIAA11281
NM_004822NTN11
NM_002318LOXL21
NM_001025091ABCF11
NM_001090ABCF11
NM_001013624LOC2011811
NM_023112OTUB21
NM_015077SARM11
NM_015231NUP1601
NM_002942ROBO21
NM_015959TXNDC141
NM_024832RIN31
NM_203290POLR1C1
NM_1735691
NM_014967MTMR151
NM_015380SAMM501
NM_033011PLAT1
NM_000930PLAT1
NM_018484SLC22A111
NM_005207CRKL1
NM_014283C1orf91
NM_016227C1orf91
NM_012264C22orf51
NM_201431RASSF61
NM_177532RASSF61
NM_022138SMOC21
NM_0209111
NM_138795ARL8A1
NM_018404CENTA21
NM_175736FMNL31
NM_198900FMNL31
NM_007283MGLL1
NM_001003794MGLL1
NM_033224PURB1
NM_015005KIAA02841
NM_0011127261
NM_015686TMEM281
NM_022720DGCR81
NM_002601PDE6D1
NM_0010992671
XM_001133370LOC7313361
XM_001134093LOC7298791
NM_006291TNFAIP21
NM_173089CAPN31
NM_173090CAPN31
NM_173088CAPN31
NM_212465CAPN31
NM_212464CAPN31
NM_000070CAPN31
NM_024344CAPN31
NM_212467CAPN31
NM_173087CAPN31
NM_018374TMEM106B1
NM_005262GFER1
NM_002241KCNJ101
NM_001712CEACAM11
NM_001024912CEACAM11
NM_024578OCEL11
NM_001785CDA1
NM_005018PDCD11
NM_020822KCNT11
NM_014665LRRC141
NM_003681PDXK1
NM_012226P2RX21
NM_174872P2RX21
NM_016318P2RX21
NM_170683P2RX21
NM_174873P2RX21
NM_170682P2RX21
NM_207380FLJ433391
NM_181785SLC46A31
NM_030806C1orf211
NM_198508KLRG21
NM_001609ACADSB1
NM_144966FREM11
NM_0010976151
NM_032959POLR2J21
NM_020638FGF231
NM_005069SIM21
NM_138468ICA1L1
NM_004419DUSP51
NM_020657ZNF3041
NM_017775TTC191
XM_001132813LOC7287411
XM_001132780LOC7287341
XM_934271LOC3882371
NM_004112FGF111
NM_021706LAIR11
NM_002287LAIR11
NM_007085FSTL11
NM_031455CCDC31
NM_181491SURF51
NM_001039884FLJ448941
NM_018990CXorf91
NM_005158ABL21
NM_007314ABL21
NM_0011001081
NM_015879ST8SIA31
NM_005171ATF11
XM_942780SYNPO21
NM_006010ARMET1
NM_001040441ZBTB81
XM_001124597LOC7304111
NM_002527NTF31
NM_0011026541
NM_001001331ATP2B21
NM_001683ATP2B21
NM_024636STEAP41
NM_000750CHRNB41
NM_022468MMP251
NM_001079559HNRPUL21
NM_052845MMAB1
NM_0010984021
NM_0010984031
NM_020727ZNF2951
NM_001080530LOC920171
NM_001008701LPHN11
NM_014921LPHN11
NM_004807HS6ST11
XM_001132928LOC7289691
NM_001039380C10orf251
NM_015463C2orf321
NM_016322RAB141
NM_020782KLHDC51
NM_181804PKIG1
NM_181805PKIG1
NM_007066PKIG1
NM_006750SNTB21
NM_001047SRD5A11
NM_017706WDR551
XM_936101LOC6420311
NM_017911FAM118A1
NM_0011045951
NM_001083924C1orf1161
NM_023938C1orf1161
NM_198526ZNF7101
NM_014743KIAA02321
NM_0011005901
NM_004247EFTUD21
NM_017634KCTD91
NM_004809STOML11
NM_001010891MTX31
NM_001010000ARHGAP281
NM_001013620ALG10B1
NM_020792AADACL11
NM_015026MON21
NM_181359IL6R1
NM_000565IL6R1
NM_015450POT11
NM_001042594POT11
NM_001126ADSS1
NM_152753SCUBE31
NM_005905SMAD91
NM_004522KIF5C1
NM_032178SLC7A6OS1
NM_032042C5orf211
NM_024701ASB131
NM_203380ACSL51
NM_203379ACSL51
NM_016234ACSL51
NM_001033602KIAA07741
NM_015233KIAA07741
NM_031886KCNA71
NM_001001420SMAD51
NM_001001419SMAD51
NM_005903SMAD51
NM_014915ANKRD261
XM_001125727LOC7277781
XM_001125757LOC7277781
NM_003137SRPK11
NM_019036HMGCLL11
NM_001042406HMGCLL11
NM_0011112831
NM_000618IGF11
NM_0011112841
NM_001079526IKZF21
NM_016260IKZF21
NM_014977ACIN11
NM_001003679LEPR1
NM_016817OAS21
NM_003418CNBP1
NM_006731FCMD1
NM_001079802FCMD1
XM_938207LOC6491251
NM_005238ETS11
NM_007259VPS451
NM_001114ADCY71
NM_005967NAB21
NM_004827ABCG21
NM_003413ZIC31
NM_001684ATP2B41
NM_005587MEF2A1
NM_152437ZNF6641
XM_001127102LOC6478591
XM_001130815LOC6478591
NM_002602PDE6G1
NM_018279TMEM191
NM_153262SYT141
NM_015257KIAA02861
NM_152588TMTC21
NM_012072CD931
NM_032330CAPNS21
NM_024969FAM130A21
NM_0010984151
NM_016596HDAC7A1
NM_0010984161
NM_015401HDAC7A1
NM_183061SLC9A101
NM_006918SC5DL1
NM_001024956SC5DL1
NM_052857CCDC161
NM_030957ADAMTS101
NM_020807ZNF3191
NM_005847SLC23A11
NM_152685SLC23A11
NM_005752CLEC3A1
NM_001007536AKR1CL11
NM_012464TLL11
NM_018416FOXJ21
NM_053282SH2D1B1
NM_0010988111
NM_033637BTRC1
NM_003939BTRC1
NM_001024647RAB3IP1
NM_022456RAB3IP1
NM_175624RAB3IP1
NM_175625RAB3IP1
NM_175623RAB3IP1
NM_015329KIAA08921
NM_024910ZNF7671
XM_378914KIAA04921
XM_944290KIAA04921
NM_152493FLJ254761
NM_001077497PRR31
NM_025263PRR31
NM_005892FMNL11
XM_938054LOC3885641
XM_498725LOC3885641
NM_021012KCNJ121
NM_002319LRCH41
NM_001039919ZNF3841
NM_001039917ZNF3841
NM_001039916ZNF3841
NM_001039920ZNF3841
NM_133476ZNF3841
NM_001039918ZNF3841
NM_181558RFC31
NM_006955ZNF33B1
NM_002645PIK3C2A1
NM_020653ZNF2871
NM_144633KCNH81
NM_020773TBC1D141
NM_182485CPEB21
NM_182646CPEB21
NM_170604RASGRP41
NM_006176NRGN1
NM_199452ZNF3651
NM_199451ZNF3651
NM_080657RSAD21
NM_007203PALM2-AKAP21
NM_147150PALM2-AKAP21
NM_001004065AKAP21
NM_024761MOBKL2B1
NM_198847FAM111A1
NM_022074FAM111A1
NM_0011003981
NM_015085GARNL41
XM_379543LOC4014421
NM_015848KRT761
NM_001005407CACNA1H1
NM_021098CACNA1H1
XM_209363SIRPB21
XM_939897SIRPB21
NM_016647C8orf551
NM_014141CNTNAP21
NM_014252SLC25A151
NM_004985KRAS1
NM_033360KRAS1
NM_033508GCK1
NM_000162GCK1
NM_033507GCK1
NM_0010994131
NM_0010994121
NM_006766MYST31
NM_000926PGR1
NM_018471ZC3H151
NM_020718USP311
NM_0011096621
NM_000361THBD1
NM_014338PISD1
NM_194247HNRPA31
XM_371655LOC3891371
NM_031905ARMC101
NM_213609FAM19A11
NM_207352CYP4V21
NM_0011006241
NM_003173SUV39H11
NM_000560CD531
NM_001040033CD531
NM_000669ADH1C1
NM_000667ADH1A1
NM_001040025ARL161
NM_017564STAB21
XM_211108LOC2835841
XM_944114LOC6502401
NM_194452RNF1211
NM_194453RNF1211
NM_018320RNF1211
NM_014309RBM91
NM_001082576RBM91
NM_001082577RBM91
NM_001031695RBM91
NM_001082578RBM91
NM_001082579RBM91
NM_001029863C6orf1201
NM_004258IGSF21
XM_934287LOC1448171
XM_945225LOC1448171
NM_014667VGLL41
NM_012409PRND1
NM_173495PTCHD11
NM_005248FGR1
NM_001042747FGR1
NM_001042729FGR1
NM_0010853751
NM_198147LOC1162361
NM_152529GPR1551
NM_001033045GPR1551
NM_001565CXCL101
NM_207454FLJ448151
NM_015084MRPS271
NM_152404UGT3A11
NM_182557BCL9L1
NM_022744C16orf581
NM_004296RGS61
NM_001013627LOC3405271
NM_0010122411
NM_020746VISA1
NM_000379XDH1
NM_199324OTUD41
NM_0011026531
NM_000459TEK1
NM_001002916H2BFWT1
NM_020122KCMF11
NM_032444BTBD121
NM_013449BAZ2A1
NM_170721MSI21
NM_001002026CLDN181
NM_016369CLDN181
NM_018423STYK11
NM_005966NAB11
NM_144988ALG141
XM_937241LOC3891371
NM_021940SMAP11
NM_001044305SMAP11
NM_206918DEGS21
NM_020228PRDM101
NM_199438PRDM101
NM_199437PRDM101
NM_199439PRDM101
NM_004926ZFP36L11
NM_001204BMPR21
NM_003679KMO1
NM_017882CLN61
NM_020648TWSG11
NM_018429BDP11
NM_017482ADD21
NM_017484ADD21
NM_000697ALOX121
NM_001033521CSTF11
NM_001033522CSTF11
NM_001324CSTF11
NM_153367C10orf561
NM_001304CPD1
NM_002146HOXB31
XM_931357LOC6426811
XM_943100LOC6426811
NM_030636KIAA17061
NM_015726WDR42A1
NM_207303ATRNL11
NM_022893BCL11A1
NM_152458ZNF7851
NM_001040665STEAP21
NM_152999STEAP21
NM_001669ARSD1
NM_014232VAMP21
NM_0152411
XM_001128811LOC7312101
NM_007050PTPRT1
NM_133170PTPRT1
NM_004948DSC11
NM_024421DSC11
NM_024086METT10D1
NM_014600EHD31
NM_173065IL28RA1
NM_001660ARF41
XM_001132763ARF41
NM_025106SPSB11
NM_183425RBM381
NM_024850BTNL81
XM_931674LOC6531171
NM_003309TSPYL11
NM_007329DMBT11
NM_004406DMBT11
NM_017579DMBT11
NM_001008493ENAH1
NM_018212ENAH1
NM_001039580MAP91
NM_207413UNQ94331
XM_939296MGA1
NM_001080541MGA1
XM_932727MGA1
XM_944092MGA1
NM_057175NARG11
NM_018287ARHGAP121
NM_003270TSPAN61
NM_182606TMPRSS11A1
NM_173531ZNF1001
NM_173808NEGR11
NM_032737LMNB21
NM_006071PKDREJ1
XM_001133842LOC7294461
NM_017680ASPN1
NM_007195POLI1
NM_001039547GK51
NM_001010895LOC3757481
NM_000953PTGDR1
NM_001015887IGSF111
NM_152538IGSF111
NM_013357PURG1
NM_014058TMPRSS11E1
NM_0010996621
NM_183050BCKDHB1
NM_005327HADH1
NM_178191ATPIF11
NM_001080978LILRB21
NM_005874LILRB21
NM_004265FADS21

Immunostimulatory motif search results


UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).

GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).



Settings used for search

Scores were calculated using scoring scheme SS1
Using AVG scoring mode.

Hit scores

ParameterSpec. Score cut-offColourComment
Hit to same geneColourHits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit.
High scoring hit≥80ColourHits are very likely to cause an off-target effect
Medium scoring hit<80¸≥40ColourHits with a small chance of causing an off target effect.
Low scoring hitColourHits very unlikely to cause an off-target effect

The mismatches are colour coded based on the weighting of that position.

Cut-offColour
MatchColour
Wobble base pairs (G-U)Colour
Critical bases (none)Colour
0.6 bitsColour
0.4 bitsColour
0.2 bitsColour

About SpecificityServer

SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).

Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer


For more details about the algorithm "siRNA specificity searching incorporating mismatch tolerance data" Bioinformatics (2008) 24 (10): 1316-1317