Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands
12 hits were found for CAGAGCAGCUGAUACUAAC (8 guide strand hits and 4 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_006071 | PKDREJ | 1052 | 1070 | C | A | G | A | G | C | A | G | C | U | G | A | U | u | C | U | g | g | a | 49 | 4 | 13 | c | false | <90% | 7660 | ||
| NM_031899 | GORASP1 | 2637 | 2655 | C | A | G | A | G | C | A | G | C | U | G | A | U | A | C | g | u | g | u | 42 | 4 | 15 | c | false | <90% | 3057 | ||
| XM_938203 | C1orf167 | 3056 | 3074 | C | A | G | A | G | C | A | G | C | U | G | c | U | A | C | U | g | c | a | 35 | 4 | 11 | false | <90% | 5515 | |||
| XM_209234 | C1orf167 | 3056 | 3074 | C | A | G | A | G | C | A | G | C | U | G | c | U | A | C | U | g | c | a | 35 | 4 | 11 | false | <90% | 5515 | |||
| NM_001159 | AOX1 | 2200 | 2218 | u | g | G | A | G | C | c | G | C | U | G | A | U | A | C | U | A | A | C | 20 | 3 | 12 | bc | false | <90% | 4949 | ||
| NR_003505 | PPP4R1L | 2030 | 2048 | C | A | G | A | a | C | A | G | C | U | G | A | U | A | C | U | g | A | u | 20 | 3 | 11 | c | false | <90% | 2398 | ||
| NM_001040152 | PEG10 | 2983 | 3001 | C | A | G | A | G | a | A | G | C | U | G | A | U | A | C | a | A | A | C | 10 | 2 | 9 | c | false | <90% | 6628 | ||
| NM_015068 | PEG10 | 2983 | 3001 | C | A | G | A | G | a | A | G | C | U | G | A | U | A | C | a | A | A | C | 10 | 2 | 9 | c | false | <90% | 6628 | ||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_147190 | LASS5 | 809 | 827 | c | c | a | A | G | U | A | U | C | A | G | C | g | G | C | U | C | U | G | 56 | 4 | 9 | false | <90% | 1986 | |||
| NR_002809 | LOC338799 | 1873 | 1891 | G | c | c | A | G | U | A | U | C | A | G | C | U | G | u | U | C | U | G | 44 | 3 | 11 | c | false | <90% | 2553 | ||
| NR_003135 | CEP170L | 61653 | 61671 | u | g | U | A | G | U | c | U | C | A | G | C | U | G | C | U | C | U | G | 20 | 3 | 12 | bc | false | <90% | 90477 | ||
| NM_022136 | SAMSN1 | 962 | 980 | G | U | U | A | c | U | A | U | C | A | G | C | U | G | C | U | g | a | a | 17 | 4 | 11 | c | false | <90% | 1893 | ||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 3359
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer