VIRsiRNAdb

SpecificityServer - An siRNA design program for determining siRNA specificity
Automatic recommendation
Warning - siRNA is specific for only the antisense (guide) strand

77 hits were found for GCGAGGAGGAAAGCAAAGA (55 guide strand hits and 22 sense strand hits.)

Hits to Guide strand

Accession

Gene

Start

End

Details of mismatches

Spec. Score

Mismatches

Contiguous

Jackson style specificity

Hits similar gene

HSP Score

HSP % Identity

HSP length

Gene Length

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

NM_147152

ITSN2

1231

1249

c

a

G

A

G

G

A

G

G

A

A

A

G

C

A

A

A

u

u

78

4

15

ac

false

<90%

4558

NM_019595

ITSN2

1231

1249

c

a

G

A

G

G

A

G

G

A

A

A

G

C

A

A

A

u

u

78

4

15

ac

false

<90%

6027

NM_006277

ITSN2

1231

1249

c

a

G

A

G

G

A

G

G

A

A

A

G

C

A

A

A

u

u

78

4

15

ac

false

<90%

6092

NM_000831

GRIK3

2552

2570

c

u

G

A

G

G

A

G

G

A

A

A

a

C

A

A

A

G

A

76

3

10

false

<90%

3733

NM_020824

ARHGAP21

695

713

c

a

G

A

G

G

A

G

G

A

A

A

a

C

A

A

A

G

A

76

3

10

false

<90%

7116

XR_016539

LOC643562

496

514

c

a

G

A

G

G

A

G

G

A

A

A

a

C

A

A

A

G

A

76

3

10

false

<90%

9549

XR_015339

LOC730736

496

514

c

a

G

A

G

G

A

G

G

A

A

A

a

C

A

A

A

G

A

76

3

10

false

<90%

9578

NM_153378

SLC22A12

2205

2223

G

g

c

A

G

G

A

G

G

A

A

A

G

C

A

A

A

G

A

69

2

16

bc

false

<90%

2940

NM_144585

SLC22A12

2471

2489

G

g

c

A

G

G

A

G

G

A

A

A

G

C

A

A

A

G

A

69

2

16

bc

false

<90%

3206

NM_022113

KIF13A

2216

2234

G

u

G

A

G

G

A

G

G

A

A

A

G

g

A

A

A

G

A

69

2

11

c

false

<90%

5878

NM_000421

KRT10

870

888

a

C

G

A

G

G

A

G

G

A

A

A

u

g

A

A

A

G

A

68

3

11

false

<90%

2145

NM_022111

CLSPN

331

349

c

C

G

A

G

G

A

G

G

A

A

A

a

u

A

A

A

G

A

68

3

11

false

<90%

4379

NM_020884

MYH7B

4195

4213

u

a

G

A

G

G

A

G

G

A

A

A

G

C

A

A

g

G

c

58

4

14

ac

false

<90%

6289

NM_000188

HK1

2947

2965

G

C

G

g

G

G

A

G

G

A

A

A

G

C

A

A

A

a

u

55

3

13

c

false

<90%

3580

NM_033496

HK1

3003

3021

G

C

G

g

G

G

A

G

G

A

A

A

G

C

A

A

A

a

u

55

3

13

c

false

<90%

3636

NM_033497

HK1

3182

3200

G

C

G

g

G

G

A

G

G

A

A

A

G

C

A

A

A

a

u

55

3

13

c

false

<90%

3815

NM_033498

HK1

3236

3254

G

C

G

g

G

G

A

G

G

A

A

A

G

C

A

A

A

a

u

55

3

13

c

false

<90%

3869

NM_033500

HK1

3329

3347

G

C

G

g

G

G

A

G

G

A

A

A

G

C

A

A

A

a

u

55

3

13

c

false

<90%

3962

NM_002454

MTRR

855

873

G

C

c

A

G

G

A

G

G

A

A

A

G

C

c

A

A

G

u

50

3

11

c

false

<90%

3259

NM_024010

MTRR

887

905

G

C

c

A

G

G

A

G

G

A

A

A

G

C

c

A

A

G

u

50

3

11

c

false

<90%

3291

NM_006231

POLE

7035

7053

a

C

G

g

G

G

A

G

G

A

A

A

c

C

A

A

A

G

A

50

3

8

false

<90%

7859

XM_936512

LOC647442

323

341

a

g

G

g

G

G

A

G

G

A

A

A

G

C

A

A

A

G

A

49

3

15

bc

false

<90%

375

XM_498110

LOC442227

323

341

a

g

G

g

G

G

A

G

G

A

A

A

G

C

A

A

A

G

A

49

3

15

bc

false

<90%

375

NM_014868

RNF10

2824

2842

G

a

a

A

G

G

A

G

G

A

A

A

G

a

A

A

A

G

A

49

3

10

c

false

<90%

3784

NM_004983

KCNJ9

1511

1529

a

g

G

A

G

G

A

G

G

A

A

g

G

C

A

A

A

G

c

47

4

9

false

<90%

3029

NM_018300

ZNF83

172

190

G

g

G

A

a

G

A

G

G

A

A

A

G

C

A

A

A

G

g

32

3

13

bc

false

<90%

2633

NM_001040143

SCN2A

3431

3449

a

u

a

u

G

G

A

G

G

A

A

A

G

C

A

A

A

G

A

30

4

15

bc

false

<90%

8571

NM_001040142

SCN2A

3670

3688

a

u

a

u

G

G

A

G

G

A

A

A

G

C

A

A

A

G

A

30

4

15

bc

false

<90%

8810

NM_021007

SCN2A

3736

3754

a

u

a

u

G

G

A

G

G

A

A

A

G

C

A

A

A

G

A

30

4

15

bc

false

<90%

8876

NM_173530

ZNF610

397

415

c

a

G

A

a

G

A

G

G

A

A

A

G

C

A

A

A

G

g

30

4

13

bc

false

<90%

1855

NM_018260

ZNF701

101

119

a

g

G

A

a

G

A

G

G

A

A

A

G

C

A

A

A

G

g

30

4

13

bc

false

<90%

2864

NM_006058

TNIP1

2159

2177

c

a

G

A

a

G

A

G

G

A

A

A

G

C

A

A

A

G

g

30

4

13

bc

false

<90%

3268

XM_937612

LOC91664

90

108

a

g

G

A

a

G

A

G

G

A

A

A

G

C

A

A

A

G

g

30

4

13

bc

false

<90%

3515

NM_001008801

ZNF468

125

143

a

g

G

A

a

G

A

G

G

A

A

A

G

C

A

A

A

G

g

30

4

13

bc

false

<90%

3935

NM_199132

ZNF468

125

143

a

g

G

A

a

G

A

G

G

A

A

A

G

C

A

A

A

G

g

30

4

13

bc

false

<90%

4053

NM_001004301

ZNF813

93

111

a

g

G

A

a

G

A

G

G

A

A

A

G

C

A

A

A

G

g

30

4

13

bc

false

<90%

4180

NM_001040185

ZNF765

97

115

a

g

G

A

a

G

A

G

G

A

A

A

G

C

A

A

A

G

g

30

4

13

bc

false

<90%

4577

NM_006969

ZNF28

94

112

a

g

G

A

a

G

A

G

G

A

A

A

G

C

A

A

A

G

g

30

4

13

bc

false

<90%

4675

NM_144684

ZNF480

74

92

a

g

G

A

a

G

A

G

G

A

A

A

G

C

A

A

A

G

g

30

4

13

bc

false

<90%

4732

NM_207333

ZNF320

165

183

a

g

G

A

a

G

A

G

G

A

A

A

G

C

A

A

A

G

g

30

4

13

bc

false

<90%

6145

NM_001004470

ST8SIA6

461

479

a

C

G

A

G

G

u

G

G

A

A

A

G

C

A

A

A

a

A

29

3

10

c

false

<90%

1398

NM_004299

ABCB7

1858

1876

a

g

G

A

G

G

A

G

a

A

A

A

G

C

A

A

A

G

A

26

3

10

bc

false

<90%

2404

NM_000087

CNGA1

427

445

a

a

G

A

G

G

A

G

a

A

A

A

G

C

A

A

A

G

A

26

3

10

bc

false

<90%

2532

NM_198682

GYPE

328

346

u

a

G

A

G

G

A

a

G

A

A

A

G

C

A

A

A

G

A

24

3

11

bc

false

<90%

1718

NM_002148

HOXD10

798

816

a

g

G

A

G

a

A

G

G

A

A

A

G

C

A

A

A

G

A

23

3

13

bc

false

<90%

1814

NM_033063

MAP6

1333

1351

a

C

a

A

G

G

A

G

c

A

A

A

G

C

A

A

A

G

A

18

3

10

bc

false

<90%

2666

NM_207577

MAP6

1333

1351

a

C

a

A

G

G

A

G

c

A

A

A

G

C

A

A

A

G

A

18

3

10

bc

false

<90%

4338

NM_016577

RAB6B

920

938

u

C

c

A

G

G

A

G

a

A

A

A

G

C

A

A

A

G

A

18

3

10

bc

false

<90%

5561

NM_007037

ADAMTS8

1264

1282

G

C

G

A

G

G

A

G

G

A

g

A

G

C

c

A

A

G

A

14

2

10

c

false

<90%

4028

NM_032180

FLJ13305

803

821

u

g

a

A

G

G

A

G

a

A

A

A

G

C

A

A

A

G

A

6

4

10

bc

false

<90%

1929

NM_203343

EPB41

386

404

c

C

G

A

G

G

A

a

G

A

A

g

G

C

A

A

A

G

A

2

3

7

false

<90%

6064

NM_004437

EPB41

369

387

c

C

G

A

G

G

A

a

G

A

A

g

G

C

A

A

A

G

A

2

3

7

false

<90%

6193

NM_203342

EPB41

386

404

c

C

G

A

G

G

A

a

G

A

A

g

G

C

A

A

A

G

A

2

3

7

false

<90%

6369

NM_152643

KNDC1

36

54

a

C

G

A

G

G

A

G

G

A

c

g

G

C

A

A

A

G

A

2

3

9

false

<90%

6773

NM_003189

TAL1

466

484

a

a

a

A

G

G

g

G

G

A

A

A

G

C

A

A

A

G

A

0

4

12

bc

false

<90%

5000

Hits to Sense strand

Accession

Gene

Start

End

Details of mismatches

Spec. Score

Mismatches

Contiguous

Jackson style specificity

Hits similar gene

HSP Score

HSP % Identity

HSP length

Gene Length

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

NM_080866

SLC22A9

1797

1815

g

C

U

U

U

G

C

U

U

U

C

C

U

C

C

U

C

c

u

90

3

16

ac

false

<90%

2327

NM_002231

CD82

511

529

a

C

U

U

U

G

C

U

U

U

C

C

U

g

C

U

C

c

u

70

4

12

c

false

<90%

1715

NM_172088

TNFSF13

582

600

a

g

c

U

U

G

C

U

U

U

C

C

U

C

C

U

C

c

C

64

4

14

c

false

<90%

2095

NM_172087

TNFSF13

582

600

a

g

c

U

U

G

C

U

U

U

C

C

U

C

C

U

C

c

C

64

4

14

c

false

<90%

2228

NM_003808

TNFSF13

582

600

a

g

c

U

U

G

C

U

U

U

C

C

U

C

C

U

C

c

C

64

4

14

c

false

<90%

2276

NM_052909

KIAA1909

10690

10708

c

u

U

U

U

G

C

U

U

U

C

C

U

C

C

U

g

G

a

58

4

14

ac

false

<90%

11507

NM_080546

SLC44A1

1952

1970

U

C

U

U

U

G

C

U

U

U

C

C

U

a

g

U

C

G

C

55

2

13

c

false

<90%

4540

NM_007243

NRM

672

690

U

C

c

U

U

G

C

U

U

U

C

C

U

C

C

U

u

a

C

54

3

13

c

false

<90%

1433

NM_021727

FADS3

554

572

U

C

U

U

U

G

C

U

U

U

C

C

U

a

C

U

g

G

g

51

3

13

c

false

<90%

1807

NM_002657

PLAGL2

4196

4214

U

C

U

U

U

c

C

U

U

U

C

C

U

C

C

U

C

u

C

34

2

11

c

false

<90%

5640

NM_016518

PIPOX

238

256

a

C

U

U

U

G

C

c

U

U

C

C

U

C

C

U

C

G

u

30

3

10

bc

false

<90%

2412

NM_153443

KIR3DL3

1019

1037

c

C

U

U

U

G

C

U

a

U

C

C

U

C

C

U

C

u

u

30

4

8

c

false

<90%

1691

NM_004487

GOLGB1

8064

8082

g

C

U

U

U

G

u

U

U

U

C

C

U

C

C

U

C

u

C

29

3

10

c

false

<90%

11185

NM_001080950

MYO1C

3216

3234

g

C

U

U

U

G

C

U

U

U

u

C

U

C

C

U

C

c

u

29

4

9

c

false

<90%

4742

NM_033375

MYO1C

3231

3249

g

C

U

U

U

G

C

U

U

U

u

C

U

C

C

U

C

c

u

29

4

9

c

false

<90%

4757

NM_001080779

MYO1C

3447

3465

g

C

U

U

U

G

C

U

U

U

u

C

U

C

C

U

C

c

u

29

4

9

c

false

<90%

4973

NM_004594

SLC9A5

843

861

U

C

U

U

U

G

C

c

U

U

C

C

U

C

C

U

g

G

C

14

2

8

c

false

<90%

3745

NM_020372

SLC22A17

937

955

c

C

U

U

U

G

C

U

U

c

C

C

U

C

C

U

C

a

a

7

4

8

c

false

<90%

2090

NM_016609

SLC22A17

1344

1362

c

C

U

U

U

G

C

U

U

c

C

C

U

C

C

U

C

a

a

7

4

8

c

false

<90%

2344

NM_001039762

FLJ45557

492

510

U

C

U

U

U

c

C

g

U

U

C

C

U

C

C

U

C

G

g

-30

3

10

bc

false

<90%

4408

XM_001132171

LOC730036

925

943

U

C

U

U

U

G

g

a

U

U

C

C

U

C

C

U

C

G

g

-33

3

10

bc

false

<90%

2479

XM_001126197

LOC730523

925

943

U

C

U

U

U

G

g

a

U

U

C

C

U

C

C

U

C

G

g

-33

3

10

bc

false

<90%

2479

---

3 prime UTR Seed matches

Hits to same gene : 0
Hits to other genes : 467
View matched accessions

3 prime seed match details

Accession	Gene name	Hits to this gene
NM_001003674	C18orf1	2
NM_181483	C18orf1	2
NM_181481	C18orf1	2
NM_001003675	C18orf1	2
NM_004338	C18orf1	2
NM_181482	C18orf1	2
NM_001012994		2
NM_001013693	LDLRAD2	2
NM_198285	WDR86	2
NM_172367	TUSC5	2
XM_001124643	LOC730416	2
NM_213568	SLC39A3	2
NM_005806	OLIG2	2
XM_001131068	LOC729696	2
XM_001129643	LOC731435	2
NM_000609	CXCL12	2
NM_153051	MTMR3	2
NM_021090	MTMR3	2
NM_153050	MTMR3	2
NM_014286	FREQ	2
NM_138793	CANT1	2
NM_052906	LRRC62	2
NM_001001872	C14orf37	1
NM_001012761	RGMB	1
NM_005654	NR2F1	1
NM_004789	LHX2	1
NM_018222	PARVA	1
NM_002860	ALDH18A1	1
NM_001017423	ALDH18A1	1
NM_144703	LSM14B	1
NM_178006	STARD13	1
NM_178007	STARD13	1
NM_052851	STARD13	1
NM_001040445	ASB1	1
NM_001608	ACADL	1
NM_002080	GOT2	1
NM_001039570	KREMEN1	1
NM_001039571	KREMEN1	1
NM_022844	MYH11	1
NM_001040113	MYH11	1
XM_938741	LOC116412	1
XM_375665	LOC116412	1
NM_020125	SLAMF8	1
XM_001125737	LOC730275	1
NM_019009	TOLLIP	1
NM_004455	EXTL1	1
NM_001076787	TP53I11	1
NM_006034	TP53I11	1
NM_002160	TNC	1
NM_207645	LOC399947	1
NM_015327	SMG5	1
NM_020795	NLGN2	1
NM_001003715	RECQL5	1
NM_001003716	RECQL5	1
NM_005259	GDF8	1
NM_201252	AFAR3	1
NM_032153	ZIC4	1
NM_012130	CLDN14	1
NM_144492	CLDN14	1
NM_006110	CD2BP2	1
NM_001037131	CENTG2	1
NM_014914	CENTG2	1
NM_148169	FBXO17	1
NM_024907	FBXO17	1
NM_178569	C5orf38	1
NM_007185	TNRC4	1
XM_943571	LOC646916	1
NM_004393	DAG1	1
NM_015488	PNKD	1
NM_022572	PNKD	1
NM_001080485	ZNF275	1
NM_134444	NLRP4	1
NM_005736	ACTR1A	1
NM_004973	JARID2	1
NM_021012	KCNJ12	1
NM_152264	SLC39A13	1
NM_130446	KLHL6	1
NM_005704	PTPRU	1
NM_133177	PTPRU	1
NM_133178	PTPRU	1
NM_002760	PRKY	1
NM_005044	PRKX	1
NM_006736	DNAJB2	1
NM_012399	PITPNB	1
XM_001132495	SLC26A11	1
NM_173626	SLC26A11	1
NM_006409	ARPC1A	1
NM_020960	GPR107	1
NM_007353	GNA12	1
NM_020773	TBC1D14	1
NM_001082575	HRNBP3	1
NM_001334	CTSO	1
NM_006177	NRL	1
NM_004729	ZBED1	1
NM_015541	LRIG1	1
NM_013435	RAX	1
NM_014470	RND1	1
NM_024709	C1orf115	1
XM_929030	LOC161527	1
XM_925864	LOC642325	1
XM_001129399	LOC642346	1
XM_928707	LOC642346	1
XM_925879	LOC642346	1
XM_001130526	LOC728636	1
XM_001130555	LOC728636	1
XM_001130543	LOC728636	1
XM_001130536	LOC728636	1
NM_001017361	ECAT1	1
NM_000719	CACNA1C	1
NM_001099682		1
NM_024859	MAGIX	1
NM_001099680		1
NM_001099681		1
NM_198074	OR2C3	1
NM_017410	HOXC13	1
NM_058192	RPUSD1	1
NM_024544	C1orf166	1
NM_203395	IYD	1
NM_018027	FRMD4A	1
NM_006393	NEBL	1
NM_152414	BHLHB5	1
XM_944216	LOC650484	1
NM_001100389		1
NM_206967	C16orf74	1
NM_022804	SNURF	1
NM_005678	SNURF	1
NM_001002836	ZNF787	1
NM_002310	LIFR	1
NM_030981	RAB1B	1
NM_198595	AFAP1	1
NM_021638	AFAP1	1
NM_002388	MCM3	1
NM_005607	PTK2	1
NM_153831	PTK2	1
NM_017540	GALNT10	1
NM_198321	GALNT10	1
NM_024831	TGS1	1
NM_001081552	SPAG11A	1
NM_058200	SPAG11B	1
NM_058202	SPAG11B	1
NM_014856		1
NM_198081	SCML4	1
NM_138983	OLIG1	1
NM_016540	GPR83	1
NM_012398	PIP5K1C	1
NM_001099289		1
NM_016642	SPTBN5	1
NM_018059	FLJ10324	1
NM_144586	LYPD1	1
NM_001077427	LYPD1	1
NM_020148	SPIRE1	1
XM_933701	LOC644165	1
XM_001129467	LOC728468	1
NM_021574	BCR	1
NM_004327	BCR	1
XM_931359	LOC338758	1
XM_944677	LOC338758	1
NM_139057	ADAMTS17	1
NM_013313	YPEL1	1
NM_130391	PTPRD	1
NM_130393	PTPRD	1
NM_130392	PTPRD	1
NM_002839	PTPRD	1
NM_001040712	PTPRD	1
NM_017444	CHRAC1	1
NM_001336	CTSZ	1
NM_001004297	OR13A1	1
NM_015286	DMN	1
NM_145728	DMN	1
XM_001131326	LOC729791	1
XM_001131187	LOC731900	1
NM_015254	KIF13B	1
XM_001130675	LOC729570	1
XM_001126968	LOC730726	1
NM_001797	CDH11	1
NM_182913	SYNE2	1
NM_015180	SYNE2	1
NM_182910	SYNE2	1
NM_182914	SYNE2	1
XM_001127257	LOC730797	1
XM_001127694	LOC728537	1
NM_004778	GPR44	1
NM_006197	PCM1	1
NM_178509	STXBP4	1
NM_001049	SSTR1	1
NM_004186	SEMA3F	1
XM_001128616	LOC401021	1
XM_001134363	FOXK2	1
XM_001134364	FOXK2	1
NM_004514	FOXK2	1
NM_015193	ARC	1
NM_002878	RAD51L3	1
NM_133629	RAD51L3	1
NM_020477	ANK1	1
NM_020475	ANK1	1
NM_000037	ANK1	1
NM_020476	ANK1	1
NM_020478	ANK1	1
NM_020480	ANK1	1
NM_198253	TERT	1
NM_198255	TERT	1
NM_001045478	LOC644997	1
NM_173595		1
NM_005400	PRKCE	1
NM_033129	SCRT2	1
NM_007084	SOX21	1
NM_001001132	ITSN1	1
NM_006924	SFRS1	1
NM_001078166	SFRS1	1
NM_020132	AGPAT3	1
NM_001037553	AGPAT3	1
NM_145301	FAM18B2	1
NM_004745	DLGAP2	1
NM_080861	SPSB3	1
NM_173527	REM2	1
NM_016545	IER5	1
NM_032538	TTBK1	1
NM_006861	RAB35	1
XM_001133211	LOC729003	1
XM_001133556	LOC729135	1
NM_153717	EVC	1
NM_003470	USP7	1
NM_000113	TOR1A	1
NM_182705	FAM101B	1
NM_020904	PLEKHA4	1
NM_032303	HSDL2	1
NM_001100423		1
NM_001100422		1
NM_001100424		1
NM_015535	LOC26010	1
NM_020745	AARS2	1
NM_032642	WNT5B	1
NM_030775	WNT5B	1
NM_004626	WNT11	1
NM_001761	CCNF	1
NM_003565	ULK1	1
XM_001133335	ULK1	1
XM_934649	C1orf34	1
XM_945958	C1orf34	1
NM_052882	ZIM3	1
XM_001131976	LOC732139	1
XM_001133550	C14orf128	1
NM_024332	BRCC3	1
NM_001018055	BRCC3	1
NM_006060	IKZF1	1
NM_053064	GNG2	1
NM_004481	GALNT2	1
NM_025219	DNAJC5	1
NM_201628	FLJ43806	1
NM_001039395	C9orf68	1
NM_031866	FZD8	1
NM_153345	TMEM139	1
NM_007014	WWP2	1
NM_199424	WWP2	1
NM_017812	CHCHD3	1
XM_001133148	LOC731291	1
XM_001132276	LOC728730	1
NM_001032386	SUOX	1
NM_001032387	SUOX	1
NM_000456	SUOX	1
NM_019856	MYOM1	1
NM_003803	MYOM1	1
NM_032174	TOMM40L	1
NM_016588	NRN1	1
NM_024949	WWC2	1
NM_022078	GPATCH3	1
NM_000578	SLC11A1	1
NM_018653	GPRC5C	1
NM_022036	GPRC5C	1
NM_181353	ID1	1
NM_002165	ID1	1
NM_020175	DUS3L	1
NM_032983	CASP2	1
NM_005957	MTHFR	1
NM_002699	POU3F1	1
NM_130459	TOR2A	1
NM_014697	NOS1AP	1
XM_001131408	LOC730350	1
XM_001127666	LOC730896	1
NM_014848	SV2B	1
NM_203373	FBXL22	1
NM_004603	STX1A	1
NM_002911	UPF1	1
NM_001080745	DGKG	1
NM_001346	DGKG	1
NM_001080744	DGKG	1
NM_152237	GAS2L1	1
XM_928029	RNF187	1
XM_001129309	RNF187	1
NM_001100607		1
NM_016186	SERPINA10	1
NM_005532	IFI27	1
NM_033018	PCTK1	1
NM_006201	PCTK1	1
NM_015241		1
XM_001128811	LOC731210	1
NM_152253	CHKB	1
NM_007079	PTP4A3	1
NM_032611	PTP4A3	1
NM_003575	ZNF282	1
NM_015210	KIAA0802	1
NM_203434	IER5L	1
NM_005550	KIFC3	1
NM_018942	HMX1	1
XM_001134414	C1orf133	1
XM_001134416	C1orf133	1
XM_926142	LOC642691	1
NM_001003800	BICD2	1
NM_015250	BICD2	1
XM_378661	C17orf51	1
XM_944416	C17orf51	1
NM_001014980	FAM132A	1
NM_007349	PAXIP1	1
NM_001008701	LPHN1	1
NM_014921	LPHN1	1
XM_379454	LOC401284	1
XM_945609	LOC401284	1
NM_005096	ZMYM3	1
NM_201599	ZMYM3	1
NM_005895	GOLGA3	1
NM_003200	TCF3	1
XM_001127233	LOC730623	1
XM_001127599	LOC728055	1
NM_001004358	FGFRL1	1
NM_021923	FGFRL1	1
NM_001004356	FGFRL1	1
NM_002501	NFIX	1
NM_198560	LHFPL4	1
NM_021168	RAB40C	1
NM_006897	HOXC9	1
NM_145285	NKX2-3	1
NM_138468	ICA1L	1
NM_004560	ROR2	1
NM_002698	POU2F2	1
NM_000068	CACNA1A	1
NM_138415	PHF21B	1
NM_016426	GTSE1	1
NM_004992	MECP2	1
NM_018943	TUBA8	1
NM_002509	NKX2-2	1
NM_019892	INPP5E	1
NM_006705	GADD45G	1
NM_000307	POU3F4	1
NM_005082	TRIM25	1
NM_153812	PHF13	1
NM_018342	TMEM144	1
NM_006653	FRS3	1
NM_080429	AQP10	1
NM_181784	SPRED2	1
NM_005961		1
NM_199131	VAX1	1
NM_001113239		1
NM_022740	HIPK2	1
NM_004364	CEBPA	1
NM_052925	LENG8	1
NM_000813	GABRB2	1
NM_021911	GABRB2	1
NM_021222	PRUNE	1
NM_001360	DHCR7	1
NM_152339	SPATA2L	1
NM_032900	ARHGAP19	1
NM_017723	C9orf167	1
NM_172069	PLEKHH2	1
NM_032358	CCDC77	1
NM_032450		1
XM_941317	KIAA1833	1
XM_932037	KIAA1833	1
NM_001003760	KLHL31	1
NM_024815	NUDT18	1
XM_001131339	LOC731034	1
XM_001127795	LOC728178	1
NM_015395	DKFZP434B0335	1
NM_014901	RNF44	1
NM_139015	SPPL3	1
NM_005434	MALL	1
NM_016272	TOB2	1
NM_024067	C7orf26	1
NM_032307	C9orf64	1
NM_018045	BSDC1	1
NM_006788	RALBP1	1
NM_194247	HNRPA3	1
XM_931281	LOC643072	1
XM_943769	LOC643072	1
NM_018053	XKR8	1
NM_147129	ALS2CL	1
NM_182775	ALS2CL	1
XM_942869	BOAT	1
XM_292512	BOAT	1
NM_001010867	C1orf69	1
NM_012304	FBXL7	1
NM_144603	NOXO1	1
NM_015353	KCTD2	1
NM_024704	C20orf23	1
NM_015329	KIAA0892	1
NM_002469	MYF6	1
NM_003955	SOCS3	1
NM_172168	NOXO1	1
NM_172167	NOXO1	1
NM_003724	JRK	1
NM_014246	CELSR1	1
NM_133334	WHSC1	1
NM_032121	DKFZp564K142	1
NM_178586	PPP2R5C	1
NM_002719	PPP2R5C	1
NM_033210	ZNF502	1
NM_001065	TNFRSF1A	1
NM_198389	PDPN	1
NM_006474	PDPN	1
NM_001006624	PDPN	1
NM_001006625	PDPN	1
NM_006609	MAP3K2	1
XM_001128799	MAP3K2	1
NM_033403		1
NM_023067	FOXL2	1
NM_001039213	CEACAM16	1
NM_030636	KIAA1706	1
NM_019055	ROBO4	1
NM_014468	VENTX	1
NM_001024943	ASL	1
NM_001024946	ASL	1
NM_001024944	ASL	1
NM_000048	ASL	1
NM_206909	PSD3	1
NM_015310	PSD3	1
XM_001132246	LOC729997	1
NM_021149	COTL1	1
NM_177401	MIDN	1
NM_012233	RAB3GAP1	1
NM_001940	ATN1	1
NM_001007026	ATN1	1
NM_013309	SLC30A4	1
NM_005828	WDR68	1
NM_001080395	AATK	1
NM_005910	MAPT	1
NM_016841	MAPT	1
NM_016834	MAPT	1
NM_016835	MAPT	1
NM_015551		1
XM_001132825	LOC730183	1
NM_174953	ATP2A3	1
NM_174956	ATP2A3	1
NM_174954	ATP2A3	1
NM_174957	ATP2A3	1
NM_174958	ATP2A3	1
NM_005173	ATP2A3	1
NM_174955	ATP2A3	1
NM_033028	BBS4	1
NM_015656		1
NM_018454	NUSAP1	1
NM_016359	NUSAP1	1
NM_016952	CDON	1
XM_001133286	CDON	1
NM_001040199	CLDND1	1
NM_001040200	CLDND1	1
NM_001040181	CLDND1	1
NM_001040183	CLDND1	1
NM_019895	CLDND1	1
NM_001040182	CLDND1	1
NM_001018057	DKK3	1
NM_013253	DKK3	1
NM_015881	DKK3	1
NM_022059	CXCL16	1
NM_024742	ARMC5	1
NM_004124	GMFB	1
NM_001083539	KIR3DS1	1
NM_013284	POLM	1
NM_001023561	ZNF749	1

---

Immunostimulatory motif search results

UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).

GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).

---

---

Settings used for search

Scores were calculated using scoring scheme SS1
Using AVG scoring mode.

Hit scores

Parameter	Spec. Score cut-off	Colour	Comment
Hit to same gene		Colour	Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit.
High scoring hit	≥80	Colour	Hits are very likely to cause an off-target effect
Medium scoring hit	<80¸≥40	Colour	Hits with a small chance of causing an off target effect.
Low scoring hit		Colour	Hits very unlikely to cause an off-target effect

The mismatches are colour coded based on the weighting of that position.

Cut-off	Colour
Match	Colour
Wobble base pairs (G-U)	Colour
Critical bases (none)	Colour
0.6 bits	Colour
0.4 bits	Colour
0.2 bits	Colour

About SpecificityServer

SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).

Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer

For more details about the algorithm "siRNA specificity searching incorporating mismatch tolerance data" Bioinformatics (2008) 24 (10): 1316-1317