Automatic recommendation
Not OK - siRNA is not specific
141 hits were found for GCAUCAUCAUCAUAAUCAA (37 guide strand hits and 104 sense strand hits.)
Hits to Guide strand
Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
NM_001240 | CCNT1 | 1874 | 1892 | u | C | A | U | C | A | U | C | A | U | C | A | U | A | A | U | C | A | c | 93 | 2 | 17 | abc | false | <90% | 2568 | ||
NM_001240 | CCNT1 | 1874 | 1892 | u | C | A | U | C | A | U | C | A | U | C | A | U | A | A | U | C | A | c | 93 | 2 | 17 | abc | false | <90% | 2568 | ||
NM_022058 | SLC4A10 | 743 | 761 | G | C | A | U | C | A | U | C | A | U | C | A | g | A | A | U | C | A | g | 84 | 2 | 12 | false | <90% | 3929 | |||
XM_001125695 | LOC727743 | 344 | 362 | c | C | A | U | C | A | U | C | A | U | C | A | U | c | A | U | C | A | c | 73 | 3 | 12 | c | false | <90% | 714 | ||
XM_001132504 | LOC732278 | 344 | 362 | c | C | A | U | C | A | U | C | A | U | C | A | U | c | A | U | C | A | c | 73 | 3 | 12 | c | false | <90% | 714 | ||
NM_152647 | C15orf33 | 1757 | 1775 | u | C | A | U | C | A | U | C | A | U | C | A | U | c | A | U | C | A | c | 73 | 3 | 12 | c | false | <90% | 1990 | ||
NM_152647 | C15orf33 | 1757 | 1775 | x | C | A | U | C | A | U | C | A | U | C | A | U | c | A | U | C | A | c | 73 | 3 | 12 | c | false | <90% | 1990 | ||
NM_178468 | FAM83C | 3009 | 3027 | u | C | A | U | C | A | U | C | A | U | C | A | U | c | A | U | C | A | u | 73 | 3 | 12 | c | false | <90% | 3188 | ||
NM_178468 | FAM83C | 3012 | 3030 | u | C | A | U | C | A | U | C | A | U | C | A | U | c | A | U | C | A | u | 73 | 3 | 12 | c | false | <90% | 3188 | ||
NM_178468 | FAM83C | 3009 | 3027 | u | C | A | U | C | A | U | C | A | U | C | A | U | c | A | U | C | A | u | 73 | 3 | 12 | c | false | <90% | 3188 | ||
NM_178468 | FAM83C | 3012 | 3030 | u | C | A | U | C | A | U | C | A | U | C | A | U | c | A | U | C | A | u | 73 | 3 | 12 | c | false | <90% | 3188 | ||
NM_207499 | FLJ41841 | 1062 | 1080 | u | C | A | U | C | A | U | C | A | U | C | A | U | c | A | U | C | A | c | 73 | 3 | 12 | c | false | <90% | 3230 | ||
XM_938097 | DKFZP686C2281 | 152 | 170 | u | C | A | U | C | A | U | C | A | U | C | A | U | c | A | U | C | A | c | 73 | 3 | 12 | c | false | <90% | 3930 | ||
XM_927469 | DKFZP686C2281 | 152 | 170 | u | C | A | U | C | A | U | C | A | U | C | A | U | c | A | U | C | A | c | 73 | 3 | 12 | c | false | <90% | 3930 | ||
NM_145003 | TSNARE1 | 1619 | 1637 | u | C | A | U | C | A | U | C | A | U | C | A | U | c | A | U | C | g | c | 70 | 4 | 12 | c | false | <90% | 1912 | ||
NM_004781 | VAMP3 | 381 | 399 | u | C | A | U | C | A | U | C | A | U | C | A | U | c | A | U | C | g | u | 70 | 4 | 12 | c | false | <90% | 2225 | ||
NM_032242 | PLXNA1 | 3009 | 3027 | c | C | A | U | C | A | U | C | A | U | C | A | a | c | A | U | C | A | A | 68 | 3 | 11 | false | <90% | 9040 | |||
NM_004858 | SLC4A8 | 835 | 853 | G | C | A | U | C | A | U | C | A | U | C | A | g | A | A | U | g | A | A | 66 | 2 | 12 | false | <90% | 4010 | |||
NM_001039960 | SLC4A8 | 835 | 853 | G | C | A | U | C | A | U | C | A | U | C | A | g | A | A | U | g | A | A | 66 | 2 | 12 | false | <90% | 5218 | |||
NM_006150 | LMO6 | 1562 | 1580 | c | C | A | c | C | A | U | C | A | U | C | A | U | A | A | U | C | A | c | 56 | 3 | 14 | bc | false | <90% | 1990 | ||
NM_002095 | GTF2E2 | 344 | 362 | u | C | A | U | C | A | U | C | A | U | C | A | U | c | g | U | C | A | A | 53 | 3 | 12 | c | false | <90% | 1560 | ||
NM_004781 | VAMP3 | 378 | 396 | u | C | u | U | C | A | U | C | A | U | C | A | U | c | A | U | C | A | u | 53 | 4 | 10 | c | false | <90% | 2225 | ||
XM_001129032 | TMEM16J | 689 | 707 | c | C | A | U | C | A | U | C | A | U | C | A | U | g | A | c | C | A | A | 52 | 3 | 12 | c | false | <90% | 2208 | ||
NM_001012302 | TMEM16J | 1251 | 1269 | c | C | A | U | C | A | U | C | A | U | C | A | U | g | A | c | C | A | A | 52 | 3 | 12 | c | false | <90% | 2879 | ||
NM_017882 | CLN6 | 450 | 468 | G | C | A | U | C | A | U | C | A | U | C | u | U | c | A | U | C | A | u | 41 | 3 | 11 | false | <90% | 2227 | |||
NM_020342 | SLC39A10 | 630 | 648 | G | C | A | U | C | A | U | C | A | U | C | u | U | g | A | U | C | A | u | 41 | 3 | 11 | false | <90% | 5231 | |||
NM_201378 | PLEC1 | 10808 | 10826 | G | C | A | U | g | A | U | C | A | U | C | A | U | c | A | U | C | A | u | 24 | 3 | 8 | c | false | <90% | 14626 | ||
NM_201381 | PLEC1 | 10828 | 10846 | G | C | A | U | g | A | U | C | A | U | C | A | U | c | A | U | C | A | u | 24 | 3 | 8 | c | false | <90% | 14646 | ||
NM_201382 | PLEC1 | 10857 | 10875 | G | C | A | U | g | A | U | C | A | U | C | A | U | c | A | U | C | A | u | 24 | 3 | 8 | c | false | <90% | 14675 | ||
NM_201383 | PLEC1 | 10871 | 10889 | G | C | A | U | g | A | U | C | A | U | C | A | U | c | A | U | C | A | u | 24 | 3 | 8 | c | false | <90% | 14689 | ||
NM_201379 | PLEC1 | 10933 | 10951 | G | C | A | U | g | A | U | C | A | U | C | A | U | c | A | U | C | A | u | 24 | 3 | 8 | c | false | <90% | 14751 | ||
NM_000445 | PLEC1 | 10937 | 10955 | G | C | A | U | g | A | U | C | A | U | C | A | U | c | A | U | C | A | u | 24 | 3 | 8 | c | false | <90% | 14755 | ||
NM_201384 | PLEC1 | 10979 | 10997 | G | C | A | U | g | A | U | C | A | U | C | A | U | c | A | U | C | A | u | 24 | 3 | 8 | c | false | <90% | 14797 | ||
NM_201380 | PLEC1 | 11431 | 11449 | G | C | A | U | g | A | U | C | A | U | C | A | U | c | A | U | C | A | u | 24 | 3 | 8 | c | false | <90% | 15249 | ||
NR_003192 | SNHG8 | 911 | 929 | u | C | A | U | u | A | U | C | A | U | C | A | U | A | A | U | g | A | A | 23 | 3 | 11 | c | false | <90% | 5904 | ||
NM_152647 | C15orf33 | 1766 | 1784 | u | C | A | U | C | A | U | C | A | c | C | A | U | c | A | U | C | A | A | -4 | 3 | 8 | c | false | <90% | 1990 | ||
NM_152647 | C15orf33 | 1766 | 1784 | u | C | A | U | C | A | U | C | A | c | C | A | U | c | A | U | C | A | A | -4 | 3 | 8 | c | false | <90% | 1990 |
Hits to Sense strand
Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
NM_018683 | ZNF313 | 656 | 674 | U | g | G | A | U | U | A | U | G | A | U | G | u | U | G | A | U | G | a | 73 | 3 | 10 | false | <90% | 2478 | |||
XR_018600 | LOC442621 | 719 | 737 | U | g | G | A | U | U | A | U | G | A | U | G | A | a | G | A | U | G | C | 69 | 2 | 11 | c | false | <90% | 974 | ||
XR_016071 | LOC729992 | 724 | 742 | U | g | G | A | U | U | A | U | G | A | U | G | A | a | G | A | U | G | C | 69 | 2 | 11 | c | false | <90% | 979 | ||
XR_017519 | LOC442730 | 591 | 609 | U | g | G | A | U | U | A | U | G | A | U | G | A | a | G | A | U | G | C | 69 | 2 | 11 | c | false | <90% | 1068 | ||
XR_018040 | LOC442730 | 591 | 609 | U | g | G | A | U | U | A | U | G | A | U | G | A | a | G | A | U | G | C | 69 | 2 | 11 | c | false | <90% | 1068 | ||
XR_018911 | LOC442730 | 591 | 609 | U | g | G | A | U | U | A | U | G | A | U | G | A | a | G | A | U | G | C | 69 | 2 | 11 | c | false | <90% | 1068 | ||
XR_016561 | LOC399950 | 628 | 646 | U | g | G | A | U | U | A | U | G | A | U | G | A | a | G | A | U | G | C | 69 | 2 | 11 | c | false | <90% | 1082 | ||
XR_018475 | LOC649058 | 633 | 651 | U | g | G | A | U | U | A | U | G | A | U | G | A | a | G | A | U | G | C | 69 | 2 | 11 | c | false | <90% | 1087 | ||
XR_016782 | LOC642818 | 622 | 640 | U | g | G | A | U | U | A | U | G | A | U | G | A | a | G | A | U | G | C | 69 | 2 | 11 | c | false | <90% | 1135 | ||
XR_019635 | LOC649079 | 622 | 640 | U | g | G | A | U | U | A | U | G | A | U | G | A | a | G | A | U | G | C | 69 | 2 | 11 | c | false | <90% | 1135 | ||
XR_019328 | LOC401648 | 616 | 634 | U | g | G | A | U | U | A | U | G | A | U | G | A | a | G | A | U | G | C | 69 | 2 | 11 | c | false | <90% | 1233 | ||
XR_017482 | LOC401648 | 616 | 634 | U | g | G | A | U | U | A | U | G | A | U | G | A | a | G | A | U | G | C | 69 | 2 | 11 | c | false | <90% | 1233 | ||
XR_015950 | LOC731777 | 713 | 731 | U | g | G | A | U | U | A | U | G | A | U | G | A | a | G | A | U | G | C | 69 | 2 | 11 | c | false | <90% | 1235 | ||
XR_017588 | LOC645515 | 713 | 731 | U | g | G | A | U | U | A | U | G | A | U | G | A | a | G | A | U | G | C | 69 | 2 | 11 | c | false | <90% | 1235 | ||
NR_002183 | FAM10A4 | 692 | 710 | U | c | G | A | U | U | A | U | G | A | U | G | A | a | G | A | U | G | C | 69 | 2 | 11 | c | false | <90% | 1599 | ||
NR_002199 | FAM10A6 | 775 | 793 | U | g | G | A | U | U | A | U | G | A | U | G | A | a | G | A | U | G | C | 69 | 2 | 11 | c | false | <90% | 1662 | ||
NR_002198 | FAM10A7 | 715 | 733 | U | g | G | A | U | U | A | U | G | A | U | G | A | a | G | A | U | G | C | 69 | 2 | 11 | c | false | <90% | 2597 | ||
NM_003932 | ST13 | 776 | 794 | U | g | G | A | U | U | A | U | G | A | U | G | A | a | G | A | U | G | C | 69 | 2 | 11 | c | false | <90% | 3214 | ||
XM_942276 | DHX40 | 992 | 1010 | U | U | G | A | U | U | A | U | G | A | U | u | A | U | G | A | U | G | u | 61 | 2 | 11 | false | <90% | 3058 | |||
NM_024612 | DHX40 | 1043 | 1061 | U | U | G | A | U | U | A | U | G | A | U | u | A | U | G | A | U | G | u | 61 | 2 | 11 | false | <90% | 3656 | |||
NM_025132 | WDR19 | 1874 | 1892 | U | U | G | c | U | U | A | U | G | A | U | G | A | U | G | A | U | a | a | 55 | 3 | 13 | c | false | <90% | 4534 | ||
XR_018825 | LOC442233 | 530 | 548 | U | U | G | A | U | U | A | U | G | A | U | G | A | U | G | g | c | u | u | 42 | 4 | 15 | c | false | <90% | 1221 | ||
XR_016429 | LOC442233 | 530 | 548 | U | U | G | A | U | U | A | U | G | A | U | G | A | U | G | g | c | u | u | 42 | 4 | 15 | c | false | <90% | 1221 | ||
NM_002158 | FOXN2 | 1380 | 1398 | U | U | G | A | U | U | A | U | G | A | a | G | A | U | G | A | U | c | C | 36 | 2 | 10 | c | false | <90% | 5472 | ||
NM_016216 | DBR1 | 1740 | 1758 | a | U | G | A | U | g | A | U | G | A | U | G | A | U | G | A | U | G | C | 34 | 2 | 13 | bc | false | <90% | 2667 | ||
NM_018307 | RHOT1 | 1058 | 1076 | U | U | G | g | U | U | A | U | G | A | U | G | A | U | G | A | c | c | u | 32 | 4 | 12 | c | false | <90% | 3089 | ||
NM_001033566 | RHOT1 | 1058 | 1076 | U | U | G | g | U | U | A | U | G | A | U | G | A | U | G | A | c | c | u | 32 | 4 | 12 | c | false | <90% | 3213 | ||
NM_001033568 | RHOT1 | 1058 | 1076 | U | U | G | g | U | U | A | U | G | A | U | G | A | U | G | A | c | c | u | 32 | 4 | 12 | c | false | <90% | 3309 | ||
NM_001009959 | KIAA1189 | 751 | 769 | U | U | G | A | U | g | A | U | G | A | U | G | A | U | G | A | U | G | a | 31 | 2 | 12 | bc | false | <90% | 3722 | ||
NM_020711 | KIAA1189 | 819 | 837 | U | U | G | A | U | g | A | U | G | A | U | G | A | U | G | A | U | G | a | 31 | 2 | 12 | bc | false | <90% | 3790 | ||
NM_002834 | PTPN11 | 3316 | 3334 | U | U | G | A | U | g | A | U | G | A | U | G | A | U | G | A | U | G | a | 31 | 2 | 12 | bc | false | <90% | 6300 | ||
XM_496355 | LOC440577 | 584 | 602 | a | U | G | A | U | g | A | U | G | A | U | G | A | U | G | A | U | G | a | 29 | 3 | 12 | bc | false | <90% | 1060 | ||
XM_001130897 | LOC440577 | 584 | 602 | a | U | G | A | U | g | A | U | G | A | U | G | A | U | G | A | U | G | a | 29 | 3 | 12 | bc | false | <90% | 1060 | ||
NM_018952 | HOXB6 | 1571 | 1589 | a | U | G | A | U | g | A | U | G | A | U | G | A | U | G | A | U | G | a | 29 | 3 | 12 | bc | false | <90% | 1686 | ||
NM_018952 | HOXB6 | 1574 | 1592 | a | U | G | A | U | g | A | U | G | A | U | G | A | U | G | A | U | G | a | 29 | 3 | 12 | bc | false | <90% | 1686 | ||
NM_018952 | HOXB6 | 1577 | 1595 | a | U | G | A | U | g | A | U | G | A | U | G | A | U | G | A | U | G | a | 29 | 3 | 12 | bc | false | <90% | 1686 | ||
NM_002044 | GALK2 | 1910 | 1928 | g | U | G | A | U | g | A | U | G | A | U | G | A | U | G | A | U | G | a | 29 | 3 | 12 | bc | false | <90% | 3121 | ||
NM_001001556 | GALK2 | 1997 | 2015 | g | U | G | A | U | g | A | U | G | A | U | G | A | U | G | A | U | G | a | 29 | 3 | 12 | bc | false | <90% | 3208 | ||
NM_000720 | CACNA1D | 110 | 128 | a | U | G | A | U | g | A | U | G | A | U | G | A | U | G | A | U | G | a | 29 | 3 | 12 | bc | false | <90% | 7193 | ||
NM_000720 | CACNA1D | 113 | 131 | a | U | G | A | U | g | A | U | G | A | U | G | A | U | G | A | U | G | a | 29 | 3 | 12 | bc | false | <90% | 7193 | ||
NM_005188 | CBL | 1493 | 1511 | a | U | G | A | U | g | A | U | G | A | U | G | A | U | G | A | U | G | a | 29 | 3 | 12 | bc | false | <90% | 11242 | ||
NM_020706 | SFRS15 | 1049 | 1067 | U | U | G | A | c | U | A | U | G | A | U | G | A | U | G | A | g | c | C | 22 | 3 | 11 | c | false | <90% | 3882 | ||
NM_018952 | HOXB6 | 1580 | 1598 | U | c | G | A | U | g | A | U | G | A | U | G | A | U | G | A | U | G | a | 20 | 3 | 12 | bc | false | <90% | 1686 | ||
NM_000720 | CACNA1D | 116 | 134 | U | g | G | A | U | g | A | U | G | A | U | G | A | U | G | A | U | G | a | 20 | 3 | 12 | bc | false | <90% | 7193 | ||
NM_178426 | ARNT | 283 | 301 | U | g | G | A | U | U | u | U | G | A | U | G | A | U | G | A | U | G | g | 17 | 3 | 11 | bc | false | <90% | 3563 | ||
NM_178427 | ARNT | 283 | 301 | U | g | G | A | U | U | u | U | G | A | U | G | A | U | G | A | U | G | g | 17 | 3 | 11 | bc | false | <90% | 4801 | ||
NM_001668 | ARNT | 283 | 301 | U | g | G | A | U | U | u | U | G | A | U | G | A | U | G | A | U | G | g | 17 | 3 | 11 | bc | false | <90% | 4846 | ||
NM_001018089 | NARG2 | 1256 | 1274 | U | g | G | A | U | U | u | U | G | A | U | G | A | U | G | A | U | G | u | 17 | 3 | 11 | bc | false | <90% | 7049 | ||
NM_024611 | NARG2 | 1416 | 1434 | U | g | G | A | U | U | u | U | G | A | U | G | A | U | G | A | U | G | u | 17 | 3 | 11 | bc | false | <90% | 7209 | ||
NM_000038 | APC | 5651 | 5669 | U | a | G | A | U | U | u | U | G | A | U | G | A | U | G | A | U | G | a | 17 | 3 | 11 | bc | false | <90% | 10719 | ||
NM_000038 | APC | 5663 | 5681 | U | a | G | A | U | U | u | U | G | A | U | G | A | U | G | A | U | G | a | 17 | 3 | 11 | bc | false | <90% | 10719 | ||
NM_014286 | FREQ | 1236 | 1254 | U | U | a | A | U | U | A | U | G | A | g | G | A | U | G | A | U | G | a | 14 | 3 | 7 | c | false | <90% | 4341 | ||
NM_015216 | HISPPD1 | 2815 | 2833 | U | U | G | A | U | a | A | U | G | A | U | G | A | U | G | A | a | c | C | 10 | 3 | 10 | c | false | <90% | 5293 | ||
XR_018268 | LOC647968 | 670 | 688 | U | U | G | A | U | U | c | U | G | A | U | G | A | a | G | A | U | G | a | 9 | 3 | 6 | c | false | <90% | 1235 | ||
XR_016578 | LOC400352 | 670 | 688 | U | U | G | A | U | U | c | U | G | A | U | G | A | a | G | A | U | G | a | 9 | 3 | 6 | c | false | <90% | 1634 | ||
NM_005791 | MPHOSPH10 | 1071 | 1089 | U | U | G | A | U | U | c | U | G | A | U | G | A | a | G | A | U | G | a | 9 | 3 | 6 | c | false | <90% | 2503 | ||
NM_145695 | DGKB | 617 | 635 | U | U | G | A | c | U | A | U | G | A | U | c | A | U | G | A | U | G | g | 9 | 3 | 6 | false | <90% | 3172 | |||
NM_004247 | EFTUD2 | 81 | 99 | U | U | G | A | U | U | c | U | G | A | U | G | A | a | G | A | U | G | a | 9 | 3 | 6 | c | false | <90% | 3332 | ||
NM_004080 | DGKB | 617 | 635 | U | U | G | A | c | U | A | U | G | A | U | c | A | U | G | A | U | G | g | 9 | 3 | 6 | false | <90% | 3926 | |||
NM_005333 | HCCS | 909 | 927 | U | U | G | A | U | U | A | U | u | A | U | G | A | U | G | g | U | G | g | 8 | 3 | 8 | c | false | <90% | 2331 | ||
NM_012143 | TFIP11 | 291 | 309 | U | U | G | A | U | g | A | U | G | A | U | G | A | U | G | A | c | G | a | 8 | 3 | 10 | c | false | <90% | 2864 | ||
NM_001008697 | TFIP11 | 368 | 386 | U | U | G | A | U | g | A | U | G | A | U | G | A | U | G | A | c | G | a | 8 | 3 | 10 | c | false | <90% | 2941 | ||
NM_000057 | BLM | 957 | 975 | U | U | G | A | U | g | A | U | G | A | U | G | A | U | u | A | U | G | a | 7 | 3 | 8 | c | false | <90% | 4528 | ||
NM_000057 | BLM | 969 | 987 | U | U | G | A | U | g | A | U | G | A | U | G | A | U | u | A | U | G | a | 7 | 3 | 8 | c | false | <90% | 4528 | ||
NM_024516 | C16orf53 | 516 | 534 | U | U | G | A | U | U | u | U | G | A | U | G | A | U | G | A | g | c | C | 7 | 3 | 9 | c | false | <90% | 1164 | ||
XM_941889 | LOC652446 | 74 | 92 | U | U | G | A | U | g | A | U | G | A | U | G | A | U | G | g | U | G | a | 5 | 3 | 9 | c | false | <90% | 306 | ||
XM_001127768 | LOC648708 | 655 | 673 | U | U | G | A | U | g | A | U | G | A | U | G | A | U | G | g | U | G | a | 5 | 3 | 9 | c | false | <90% | 786 | ||
XR_018527 | LOC648708 | 643 | 661 | U | U | G | A | U | g | A | U | G | A | U | G | A | U | G | g | U | G | a | 5 | 3 | 9 | c | false | <90% | 1250 | ||
XR_018384 | LOC648269 | 742 | 760 | U | U | G | A | U | g | A | U | G | A | U | G | A | U | G | g | U | G | a | 5 | 3 | 9 | c | false | <90% | 1334 | ||
XM_290482 | FAM21B | 1088 | 1106 | U | U | G | A | U | g | A | U | G | A | U | G | A | U | G | g | U | G | a | 5 | 3 | 9 | c | false | <90% | 4326 | ||
NM_000057 | BLM | 1777 | 1795 | U | U | G | A | U | g | A | U | G | A | U | G | A | U | G | A | c | u | g | 5 | 4 | 10 | c | false | <90% | 4528 | ||
NM_015262 | FAM21C | 1451 | 1469 | U | U | G | A | U | g | A | U | G | A | U | G | A | U | G | g | U | G | a | 5 | 3 | 9 | c | false | <90% | 4621 | ||
NM_001005751 | LOC387680 | 1434 | 1452 | U | U | G | A | U | g | A | U | G | A | U | G | A | U | G | g | U | G | a | 5 | 3 | 9 | c | false | <90% | 4735 | ||
NM_000038 | APC | 5645 | 5663 | U | U | G | A | U | g | A | U | G | A | U | G | A | U | G | u | U | G | a | 5 | 3 | 9 | c | false | <90% | 10719 | ||
NM_000038 | APC | 5657 | 5675 | U | U | G | A | U | g | A | U | G | A | U | G | A | U | G | u | U | G | a | 5 | 3 | 9 | c | false | <90% | 10719 | ||
NM_001219 | CALU | 197 | 215 | U | U | G | A | U | U | A | U | G | A | c | c | A | U | G | A | U | G | C | 4 | 2 | 10 | false | <90% | 3320 | |||
XR_019515 | LOC339858 | 119 | 137 | a | a | c | A | U | g | A | U | G | A | U | G | A | U | G | A | U | G | C | 3 | 4 | 13 | bc | false | <90% | 574 | ||
XR_017649 | LOC339858 | 119 | 137 | a | a | c | A | U | g | A | U | G | A | U | G | A | U | G | A | U | G | C | 3 | 4 | 13 | bc | false | <90% | 574 | ||
XM_001131613 | LOC729882 | 642 | 660 | a | c | a | A | U | g | A | U | G | A | U | G | A | U | G | A | U | G | C | 3 | 4 | 13 | bc | false | <90% | 687 | ||
XM_001130448 | LOC731692 | 642 | 660 | a | c | a | A | U | g | A | U | G | A | U | G | A | U | G | A | U | G | C | 3 | 4 | 13 | bc | false | <90% | 687 | ||
NM_020901 | KIAA1542 | 428 | 446 | U | c | a | A | U | U | c | U | G | A | U | G | A | U | G | A | U | G | C | 2 | 3 | 12 | bc | false | <90% | 5521 | ||
NM_005021 | ENPP3 | 2335 | 2353 | U | U | G | A | U | U | A | U | a | A | U | u | A | U | G | A | U | G | g | 1 | 3 | 8 | false | <90% | 3180 | |||
NM_001002909 | GPATCH8 | 328 | 346 | U | U | G | A | U | U | A | U | G | c | U | G | A | a | G | A | U | G | C | -2 | 2 | 9 | c | false | <90% | 6877 | ||
NM_138467 | TYW3 | 808 | 826 | U | U | G | A | a | a | A | U | G | A | U | G | A | U | G | A | U | G | a | -19 | 3 | 12 | bc | false | <90% | 2380 | ||
NM_004537 | NAP1L1 | 1164 | 1182 | U | U | G | A | a | g | A | U | G | A | U | G | A | U | G | A | U | G | a | -19 | 3 | 12 | bc | false | <90% | 2908 | ||
NM_139207 | NAP1L1 | 1164 | 1182 | U | U | G | A | a | g | A | U | G | A | U | G | A | U | G | A | U | G | a | -19 | 3 | 12 | bc | false | <90% | 3582 | ||
XM_001131613 | LOC729882 | 645 | 663 | a | U | G | A | c | a | A | U | G | A | U | G | A | U | G | A | U | G | a | -21 | 4 | 12 | bc | false | <90% | 687 | ||
XM_001130448 | LOC731692 | 645 | 663 | a | U | G | A | c | a | A | U | G | A | U | G | A | U | G | A | U | G | a | -21 | 4 | 12 | bc | false | <90% | 687 | ||
NM_025139 | ARMC9 | 1812 | 1830 | U | U | G | A | a | U | c | U | G | A | U | G | A | U | G | A | U | G | a | -22 | 3 | 11 | bc | false | <90% | 2178 | ||
NM_000057 | BLM | 963 | 981 | U | U | G | A | a | U | u | U | G | A | U | G | A | U | G | A | U | G | a | -22 | 3 | 11 | bc | false | <90% | 4528 | ||
NM_000057 | BLM | 975 | 993 | U | U | G | A | a | U | u | U | G | A | U | G | A | U | G | A | U | G | a | -22 | 3 | 11 | bc | false | <90% | 4528 | ||
NM_052958 | C8orf34 | 2333 | 2351 | U | U | G | A | U | a | A | U | G | A | a | G | A | U | G | A | U | G | C | -24 | 2 | 8 | c | false | <90% | 3669 | ||
NM_000057 | BLM | 1783 | 1801 | a | U | G | A | c | U | u | U | G | A | U | G | A | U | G | A | U | G | a | -24 | 4 | 11 | bc | false | <90% | 4528 | ||
XM_001133539 | LOC732427 | 17 | 35 | U | U | G | A | U | g | A | U | G | A | g | G | A | U | G | A | U | G | g | -29 | 3 | 7 | c | false | <90% | 432 | ||
NM_017637 | BNC2 | 2098 | 2116 | U | U | G | A | U | g | A | U | G | A | a | G | A | U | G | A | U | G | a | -29 | 3 | 7 | c | false | <90% | 12926 | ||
XM_496355 | LOC440577 | 593 | 611 | U | U | G | A | U | g | A | a | G | A | U | G | A | U | G | A | U | G | a | -30 | 3 | 10 | bc | false | <90% | 1060 | ||
XM_001130897 | LOC440577 | 593 | 611 | U | U | G | A | U | g | A | a | G | A | U | G | A | U | G | A | U | G | a | -30 | 3 | 10 | bc | false | <90% | 1060 | ||
NM_000350 | ABCA4 | 5848 | 5866 | U | U | G | A | U | g | A | a | G | A | U | G | A | U | G | A | U | G | u | -30 | 3 | 10 | bc | false | <90% | 7326 | ||
NM_020818 | KIAA1409 | 2490 | 2508 | U | U | G | A | U | U | g | U | G | A | a | G | A | U | G | A | U | G | a | -32 | 3 | 7 | c | false | <90% | 8471 | ||
NM_001032396 | PJA1 | 784 | 802 | U | U | G | A | U | a | c | U | G | A | U | G | A | U | G | A | U | G | a | -34 | 3 | 11 | bc | false | <90% | 2402 | ||
NM_145119 | PJA1 | 1110 | 1128 | U | U | G | A | U | a | c | U | G | A | U | G | A | U | G | A | U | G | a | -34 | 3 | 11 | bc | false | <90% | 2728 | ||
NM_002044 | GALK2 | 1919 | 1937 | U | U | G | A | U | g | A | U | G | g | U | G | A | U | G | A | U | G | a | -51 | 3 | 8 | bc | false | <90% | 3121 | ||
NM_001001556 | GALK2 | 2006 | 2024 | U | U | G | A | U | g | A | U | G | g | U | G | A | U | G | A | U | G | a | -51 | 3 | 8 | bc | false | <90% | 3208 | ||
NR_002836 | PGM5P2 | 1639 | 1657 | U | U | G | A | U | g | A | U | G | g | U | G | A | U | G | A | U | G | u | -51 | 3 | 8 | bc | false | <90% | 1988 |
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 1480
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
Parameter | Spec. Score cut-off | Colour | Comment |
Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
Cut-off | Colour |
Match | Colour |
Wobble base pairs (G-U) | Colour |
Critical bases (none) | Colour |
0.6 bits | Colour |
0.4 bits | Colour |
0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer