Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands
9 hits were found for CCAAGAUUUAUGAGUGUAA (8 guide strand hits and 1 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_025184 | EFHC2 | 579 | 597 | u | C | A | A | G | A | U | U | U | A | U | G | A | c | U | G | U | g | A | 75 | 3 | 12 | c | false | <90% | 2511 | ||
| NM_001010851 | ZNF766 | 426 | 444 | g | g | A | A | G | A | U | U | U | A | U | G | A | a | U | G | U | A | A | 67 | 3 | 11 | c | false | <90% | 2975 | ||
| XM_091886 | FLJ16287 | 850 | 868 | g | g | A | A | a | A | U | U | U | A | U | G | A | G | U | G | U | A | A | 35 | 3 | 14 | bc | false | <90% | 3185 | ||
| XM_937268 | FLJ16287 | 850 | 868 | g | g | A | A | a | A | U | U | U | A | U | G | A | G | U | G | U | A | A | 35 | 3 | 14 | bc | false | <90% | 3185 | ||
| NM_001039891 | ZNF674 | 1356 | 1374 | a | g | A | A | c | A | U | U | U | A | U | G | A | G | U | G | U | A | g | 30 | 4 | 13 | bc | false | <90% | 2689 | ||
| NM_001010879 | ZIK1 | 804 | 822 | a | a | A | A | G | c | U | U | U | A | U | G | A | G | U | G | U | A | g | 18 | 4 | 12 | bc | false | <90% | 2676 | ||
| NM_018247 | TMEM30A | 1618 | 1636 | a | C | A | A | G | A | U | g | U | A | U | G | A | G | U | a | U | A | A | 9 | 3 | 7 | c | false | <90% | 4410 | ||
| NM_024942 | C10orf88 | 945 | 963 | C | C | A | A | G | c | U | U | g | A | U | G | A | G | U | G | U | A | A | -23 | 2 | 10 | bc | false | <90% | 2334 | ||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NR_002819 | MALAT1 | 2333 | 2351 | U | U | A | u | A | C | U | C | A | U | g | A | A | U | C | U | U | G | u | -3 | 3 | 7 | c | false | <90% | 8352 | ||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 2225
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer