Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands
10 hits were found for GCUUAUAGGACAACAAAUU (6 guide strand hits and 4 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_004932 | CDH6 | 2371 | 2389 | c | C | a | U | A | g | A | G | G | A | C | A | A | C | A | A | A | U | U | 15 | 3 | 13 | bc | false | <90% | 4521 | ||
| NM_002545 | OPCML | 4036 | 4054 | G | C | a | U | A | U | A | G | a | A | C | A | A | C | A | A | A | U | g | 15 | 3 | 9 | bc | false | <90% | 6300 | ||
| NM_001012393 | OPCML | 4149 | 4167 | G | C | a | U | A | U | A | G | a | A | C | A | A | C | A | A | A | U | g | 15 | 3 | 9 | bc | false | <90% | 6413 | ||
| NM_020890 | KIAA1524 | 1409 | 1427 | u | C | U | U | A | U | A | G | a | A | C | A | A | C | A | A | u | U | U | 14 | 3 | 7 | c | false | <90% | 3395 | ||
| NM_004944 | DNASE1L3 | 1196 | 1214 | G | C | U | U | A | g | A | G | G | A | C | A | A | g | A | A | A | U | c | 12 | 3 | 7 | c | false | <90% | 1463 | ||
| NM_152269 | C12orf65 | 1258 | 1276 | a | C | U | U | A | a | A | a | G | A | C | A | A | C | A | A | A | U | U | -27 | 3 | 11 | bc | false | <90% | 2071 | ||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NR_002771 | DLEU2L | 1481 | 1499 | u | A | U | U | U | G | U | U | G | U | C | C | c | A | U | A | A | u | u | 79 | 4 | 11 | false | <90% | 1657 | |||
| NM_014141 | CNTNAP2 | 5008 | 5026 | c | c | U | U | U | G | a | U | G | U | C | C | U | A | U | A | A | G | u | 15 | 4 | 11 | bc | false | <90% | 9890 | ||
| NM_024665 | TBL1XR1 | 4668 | 4686 | u | A | U | U | U | G | U | U | G | U | u | g | U | A | U | A | A | G | C | 2 | 3 | 9 | false | <90% | 5911 | |||
| NM_015091 | KIAA0423 | 5670 | 5688 | A | A | U | U | U | G | U | U | G | c | C | C | U | A | U | A | u | G | u | -11 | 3 | 9 | c | false | <90% | 6257 | ||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 1321
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer