Predicted Off-targets for siRNA "gguacauguucgagaguaa" Using siRNA SpecificityServer

Specificity Server [Result page v1.0]


SpecificityServer - An siRNA design program for determining siRNA specificity

Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands

2 hits were found for GGUACAUGUUCGAGAGUAA (2 guide strand hits and 0 sense strand hits.)

Hits to Guide strand

AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
XM_371195 C19orf16 316 334 uGccCAUGUUCGAGAGUAA41 3 15 bc false   <90%   2282
XM_938028 C19orf16 316 334 uGccCAUGUUCGAGAGUAA41 3 15 bc false   <90%   3186



3 prime UTR Seed matches

Hits to same gene : 0
Hits to other genes : 1046
View matched accessions

3 prime seed match details

AccessionGene nameHits to this gene
NM_005756GPR643
NM_001079859GPR643
NM_001079858GPR643
NM_001079860GPR643
XM_927706LOC6445913
XM_927767LOC6535053
XM_928338LOC6535983
NM_015852ZNF1172
NM_017455NPTN2
NM_012428NPTN2
NM_014911AAK12
XM_933935LOC6467782
XM_943239LOC6467782
NM_052811TRIM132
NM_213590TRIM132
NM_001007278TRIM132
NM_005798TRIM132
NM_001004349FLJ454222
NM_182898CREB52
NM_004904CREB52
NM_001011666CREB52
NM_182899CREB52
NM_002977SCN9A2
NM_138468ICA1L2
NM_001079526IKZF22
NM_016260IKZF22
NM_020772NUFIP22
NM_003417ZNF2642
NM_178509STXBP42
NM_020297ABCC92
NM_173598KSR22
NM_053042KIAA17292
NM_0011024212
NM_0011024202
NM_006007ZFAND52
NM_021961TEAD12
NM_000134FABP22
NM_001015882bA16L21.2.12
NM_003034ST8SIA12
NM_153208IQCK2
NM_021191NEUROD42
NM_032295SLC37A32
NM_207113SLC37A32
NM_002725PRELP2
NM_201348PRELP2
NM_018018SLC38A42
XM_001126254LOC7305382
XM_001131842LOC7303582
XM_001131526LOC7298522
NM_014423AFF42
NM_014155ZBTB442
NM_0152072
NM_181489ZNF4452
NM_006281STK32
NM_144590ANKRD222
NM_013255MKLN12
NM_016079VPS242
NM_001005753VPS242
NM_0209112
NM_207577MAP62
NM_001939DRP22
NM_173214NFAT52
NM_006599NFAT52
NM_138714NFAT52
NM_0011131782
NM_138713NFAT52
NM_152785GCET22
NM_001008756GCET22
NM_016424CROP2
NM_005465AKT32
NM_032208ANTXR11
NM_006540NCOA21
NM_032507PGBD11
NM_001010853ACY1L21
NM_000834GRIN2B1
NM_176824BBS71
NM_019071ING31
NM_007195POLI1
NM_006348COG51
NM_181733COG51
NM_012395PFTK11
NM_003887DDEF21
NM_133477SYNPO21
XM_942776SYNPO21
XM_936336SYNPO21
NM_001002001GMPR21
NM_016576GMPR21
NM_001002000GMPR21
NM_001002002GMPR21
NM_015713RRM2B1
NM_002214ITGB81
NM_001042444CAST1
NM_001042440CAST1
NM_001042446CAST1
NM_001042441CAST1
NM_001750CAST1
NM_173063CAST1
NM_001042443CAST1
NM_001042442CAST1
NM_173060CAST1
NM_173061CAST1
NM_001042445CAST1
NM_005454CER11
NM_172239REXO1L11
NM_014953DIS31
XM_001129365LOC7313681
XM_001128918LOC7289611
NM_001455FOXO3A1
NM_201559FOXO3A1
NM_030899ZNF3231
NM_145909ZNF3231
NM_025015HSPA12A1
NM_001351DAZL1
NM_021204ENOPH11
NM_001025079CD471
NM_001777CD471
NM_198793CD471
XM_938440LOC4006821
NM_173531ZNF1001
NM_182515ZNF7141
NM_030791SGPP11
NM_005779LHFPL21
XM_940430LOC6488521
NM_001241CCNT21
NM_058241CCNT21
NM_198261RSRC21
NM_198262RSRC21
NM_023012RSRC21
NM_014883FAM13A11
NM_001015045FAM13A11
NM_015025MYT1L1
NM_002843PTPRJ1
NM_032141CCDC551
NM_080552SLC32A11
NM_153367C10orf561
NM_018115SDAD11
NM_198277SLC37A21
NM_005870SAP181
NM_022898BCL11B1
NM_138576BCL11B1
NM_001981EPS151
NM_005431XRCC21
NM_178448C9orf1401
NM_015387PREI31
NM_199482PREI31
NM_0011008191
NM_001043318C14orf431
NM_194278C14orf431
XM_001130012LOC7315471
XM_001129569LOC7291771
NM_001080391SP1001
NM_024553CCDC1321
NM_005082TRIM251
NM_018306TMEM401
NM_007073BVES1
NM_147147BVES1
NM_014368LHX61
NM_199160LHX61
NM_000462UBE3A1
NM_130839UBE3A1
NM_130838UBE3A1
NM_006379SEMA3C1
NM_018265C1orf1061
NM_001024465SOD21
NM_001024466SOD21
NM_018947CYCS1
NM_001001872C14orf371
NM_003808TNFSF131
NM_172087TNFSF131
NM_172089TNFSF12-TNFSF131
NM_198517TBC1D10C1
NM_138786TM4SF181
XM_001129643LOC7314351
XM_001131068LOC7296961
NM_021615CHST61
NM_015938NMD31
NM_024811FLJ125291
NM_017759FLJ203091
XM_001129261LOC7290821
XM_001129056LOC7312731
NM_001043SLC6A21
NM_015365AMMECR11
NM_001025580AMMECR11
NM_199188LARP41
NM_052879LARP41
NM_199190LARP41
NM_025218ULBP11
NM_000444PHEX1
NM_017899TESC1
NM_032195SON1
NM_181877ZSCAN21
NM_145011ZNF251
NM_000910NPY2R1
NM_0011023961
NM_025073SIKE1
NM_001039547GK51
NM_024613PLEKHF21
NM_003339UBE2D21
NM_181838UBE2D21
NM_194300CCDC1291
NM_014646LPIN21
NM_002836PTPRA1
NM_080840PTPRA1
NM_080841PTPRA1
NM_177398LMX1A1
NM_001033507LMX1A1
NM_177399LMX1A1
NM_005504BCAT11
NM_018362LIN7C1
NM_004257TGFBRAP11
NM_033303ADRA1A1
NM_016195MPHOSPH11
NM_170725PGBD21
NM_001017434PGBD21
NM_003423ZNF431
NM_138286ZNF6811
NM_182524ZNF5951
NM_021148ZNF2731
NM_015197PACS21
NM_0011009131
NM_032927TMEM1281
NM_001012509SLC45A21
NM_022492TTC311
NM_032154PCGF61
NM_001011663PCGF61
NM_139164STARD41
NM_002393MDM41
NM_003070SMARCA21
NM_139045SMARCA21
NM_145283C9orf1211
NM_0011024061
NM_025144ALPK11
NM_144684ZNF4801
NM_153018ZFP31
NM_032869NUDCD11
NM_014217KCNK21
NM_001017424KCNK21
NM_001017425KCNK21
NM_015523REXO21
NM_001001664LOC3397451
NM_004442EPHB21
NM_017449EPHB21
NM_152869RGN1
NM_004683RGN1
NM_014518ZNF2291
NM_001024858SPTB1
NM_058191C21orf661
NM_017634KCTD91
NM_207428C11orf551
NM_021947SRR1
NM_006071PKDREJ1
NM_006955ZNF33B1
NM_006974ZNF33A1
NM_006954ZNF33A1
NM_020715PLEKHH11
NM_018211RAVER21
NM_172016TRIM391
NM_021253TRIM391
NM_134431SLCO1A21
NM_001083625ANKRD121
NM_015208ANKRD121
NM_014683ULK21
NM_000824GLRB1
NM_0011128001
NM_021097SLC8A11
NM_0011128011
NM_0011128021
NM_003503CDC71
NM_0010984841
NM_003759SLC4A41
NM_001106ACVR2B1
NM_004958FRAP11
NM_020932MAGEE11
NM_173552C3orf581
NM_001781CD691
NM_031844HNRPU1
NM_004501HNRPU1
NM_0190041
NM_180991SLCO4C11
NM_001017992DKFZp686D09721
NM_018214LRRC11
NM_001025252TPD521
NM_001025253TPD521
NM_005079TPD521
NM_017420SIX41
NM_005099ADAMTS41
XM_001127312LOC7283601
XM_001129734LOC7314601
NM_014573TMEM971
NM_001003819TRIM6-TRIM341
NM_001003827TRIM341
NM_021616TRIM341
NM_130389TRIM341
NM_182757IBRDC21
NM_000809GABRA41
NM_004417DUSP11
NM_024529CDC731
NM_006420ARFGEF21
NM_052905FMNL21
NM_024913FLJ219861
NM_138383ABBA-11
NM_145173DIRAS11
NM_001040647RCP91
NM_001040648RCP91
NM_014478RCP91
NM_199204DHRS91
NM_005771DHRS91
NM_001039380C10orf251
NM_020731AHRR1
NM_173664ARL101
NM_020993BCL7A1
NM_001024808BCL7A1
NM_001001694FLJ419931
NM_020448NPAL31
NM_024639ZNF322A1
NM_199005ZNF322B1
NM_018322C6orf641
NM_0010988441
NM_014749KIAA05861
NM_004747DLG51
NM_005898CAPRIN11
NM_006933SLC5A31
NM_002748MAPK61
NM_018298MCOLN31
NM_005406ROCK11
NM_003659AGPS1
NM_004782SNAP291
NM_003702RGS201
NM_170587RGS201
NM_017540GALNT101
NM_198321GALNT101
NM_001024596ZNF7721
NM_002598PDCD21
NM_030806C1orf211
NM_007050PTPRT1
NM_133170PTPRT1
NM_001364DLG21
NM_001083621ZBTB401
NM_014870ZBTB401
NM_024619FN3KRP1
NM_001018067SERBP11
NM_015640SERBP11
NM_001018069SERBP11
NM_001018068SERBP11
NM_004554NFATC41
NM_002536TBC1D251
NM_033082CIP291
NM_030968C1QTNF11
NM_198594C1QTNF11
NM_198593C1QTNF11
XM_001131899LOC2839041
NM_207468FLJ435051
NM_007283MGLL1
NM_001003794MGLL1
NM_207517ADAMTSL31
NM_001009611PRAMEF41
NM_001010890PRAMEF91
XM_497653LOC4418731
XM_928401LOC6453591
XM_001130065LOC7293681
NM_001013407PRAMEF51
NM_001010889PRAMEF61
XM_372050HNRPA1P41
XM_939887HNRPA1P41
XM_944835LOC6486911
NM_031453FAM107B1
NM_145177DHRSX1
NM_012402ARFIP21
NM_014408TRAPPC31
NM_003791MBTPS11
NM_032983CASP21
NM_014240LIMD11
NM_015321CRTC11
NM_0010984821
NM_002048GAS11
NM_014751MTSS11
NM_002213ITGB51
XM_001129224ITGB51
NM_001042508LOC6534831
NM_003631PARG1
XM_001124642LOC7277261
XM_937616PARG1
NM_019080NDFIP21
NM_032750ABHD14B1
NM_213654ARMC81
NM_014154ARMC81
NM_030774OR51E21
NM_152624DCP21
NM_001039580MAP91
NM_019903ADD31
NM_016824ADD31
NM_001121ADD31
NM_022166XYLT11
XM_001125986LOC2838241
NM_021633KLHL121
NM_001077204CSPP11
NM_024790CSPP11
NM_145861EDARADD1
NM_080738EDARADD1
NM_148957TNFRSF191
NM_020973GBA31
NM_012062DNM1L1
NM_001025266LOC2853821
NM_000808GABRA31
NM_0155661
NM_006106YAP11
NM_153343ENPP61
NM_021255PELI21
NM_001004351MGC573591
NM_016400HYPK1
NM_144967FLJ300581
NM_015386COG41
XM_001134051FLJ320651
XM_001134063FLJ320651
NM_006454MXD41
NM_020954KIAA16181
XM_001126629LOC7306341
XM_001131395LOC7298101
NM_172337OTX21
NM_021728OTX21
NM_006451PAIP11
NM_182789PAIP11
NM_183323PAIP11
NM_007136ZNF801
NM_173177C1D1
NM_006333C1D1
XM_942964LOC2864111
XM_932619LOC2864111
NM_001039709CBFA2T21
NM_001032999CBFA2T21
NM_005093CBFA2T21
NM_018299UBE2W1
NM_001001481UBE2W1
NM_178840C1orf641
NM_014232VAMP21
XM_932515MIA31
XM_943832MIA31
NM_015565ZNF2941
NM_000961PTGIS1
NM_018638ETNK11
XM_001126406LOC7279221
XM_001126391LOC7279221
XM_001126417LOC7279221
NM_016586MBIP1
NM_022735ACBD31
NM_182826SCARA31
NM_001042493C6orf1621
NM_020425C6orf1621
NM_030755TXNDC11
NM_005813PRKD31
NM_152756RICTOR1
NM_001083614EARS21
XM_931601LOC6435171
XM_943373LOC6435171
NM_016530RAB8B1
NM_003576STK241
NM_001032296STK241
NM_003463PTP4A11
NM_017637BNC21
NM_058004PIK4CA1
NM_002650PIK4CA1
NM_198289CIDEA1
NM_001279CIDEA1
NM_014730KIAA01521
NM_006476ATP5L1
NM_0011046471
NM_018155SLC25A361
NM_005171ATF11
NM_0011045861
NM_024744ALS2CR81
NM_182703ANKDD1A1
NM_001679ATP1B31
XM_001133534ATP1B31
XM_001133533ATP1B31
NM_0011127361
NM_015224C3orf631
NM_014186COMMD91
NM_0011016531
NM_024551ADIPOR21
NM_170774RASSF21
NM_014737RASSF21
NM_018509LRRC591
NM_032439PHYHIPL1
NM_213589RAPH11
NM_014817KIAA06441
NM_024674LIN281
NM_173807C1orf1011
NM_015084MRPS271
NM_014309RBM91
NM_001082576RBM91
NM_001082577RBM91
NM_001031695RBM91
NM_001082578RBM91
NM_001082579RBM91
NM_004575POU4F21
NM_012426SF3B31
NM_213651SLC25A241
NM_013386SLC25A241
NM_001508GPR391
NM_005885MARCH61
NM_004738VAPB1
NM_033028BBS41
NM_020662MRS2L1
NM_006166NFYB1
NM_001010932HGF1
NM_000601HGF1
NM_004859CLTC1
NM_013238DNAJC151
NM_001004318FLJ161651
NM_0010976361
NM_0010976341
NM_0010976351
NM_001490GCNT11
NM_0010976331
NM_032294CAMKK11
NM_172206CAMKK11
NM_006775QKI1
NM_024628SLC12A81
NM_016173HEMK11
NM_016575NT5DC31
NM_024989PGAP11
NM_000692ALDH1B11
NM_170740ALDH5A11
NM_001080ALDH5A11
NM_025187C16orf701
NM_018979WNK11
NM_024780TMC51
NM_0011052491
NM_0011052481
NM_020432PHTF21
XM_001126151LOC7278581
XM_001130844LOC7287641
NM_000747CHRNB11
NM_0010839461
NM_144736PRO18531
NM_014396VPS411
NM_080631VPS411
NM_033107GTPBP101
NM_001042717GTPBP101
NM_006682FGL21
NM_014208DSPP1
NM_014014ASCC3L11
NM_002027FNTA1
NM_001018676FNTA1
NM_001018677FNTA1
NM_000232SGCB1
NM_001047605KIR3DX11
NM_023929ZBTB101
NM_0011055391
NM_004697PRPF41
NM_015910LOC510571
NM_001042692LOC510571
NM_000368TSC11
NM_0149811
NM_018120ARMC11
NM_003856IL1RL11
NM_014982PCNX1
NM_052950WDFY21
NM_0251851
NM_138972BACE11
NM_012104BACE11
NM_138973BACE11
NM_138971BACE11
NM_001040169HTR41
NM_199453HTR41
NM_012409PRND1
NM_173562KCTD201
NM_012431SEMA3E1
NM_016376ANKFY11
NM_005056JARID1A1
NM_001042603JARID1A1
NM_177456MAGEC31
NM_006774INMT1
NM_016371HSD17B71
XM_001123478LOC7304121
NM_022918TMEM1351
NM_024795TM4SF201
NM_003436ZNF1351
NM_001066TNFRSF1B1
NM_001961EEF21
NM_194250ZNF804A1
NM_001007267PLA2R11
NM_002486NCBP11
NM_018351FGD61
NM_025104DBF4B1
NM_004572PKP21
NM_001005242PKP21
NM_015557CHD51
NM_018364RSBN11
NM_021258IL22RA11
NM_014777KIAA01331
XM_001128800LOC7283511
XM_001130904LOC7309591
XM_933931LOC6467741
XM_945519LOC6524491
NM_0010988151
NM_005018PDCD11
NM_022753S100PBP1
NM_014613UBXD81
NM_030915LBH1
XM_001132517LBH1
XM_001132511LBH1
NM_020676ABHD61
NM_021965PGM51
NM_001322CST21
NM_001899CST41
NM_001898CST11
NM_022901LRRC191
NM_005472KCNE31
NM_182526C14orf831
XM_001125875LOC7278181
NM_024997ATF7IP21
NM_000429MAT1A1
NM_024080TRPM81
NM_003644GAS71
NM_201432GAS71
NM_201433GAS71
NM_001379DNMT11
NM_024332BRCC31
NM_001018055BRCC31
NM_203395IYD1
NM_0011113191
NM_003615SLC4A71
NM_001033578SGK31
NM_013257SGK31
NM_170709SGK31
NM_001079906ZNF3311
NM_018555ZNF3311
NM_001079907ZNF3311
NM_003216TEF1
NM_001012981ZKSCAN21
NM_206909PSD31
NM_015310PSD31
NM_004760STK17A1
NM_018330KIAA15981
NM_0010400111
NM_031418TMEM16C1
NM_001002814RAB11FIP11
NM_015715PLA2G31
NM_018683ZNF3131
NM_004464FGF51
NM_033143FGF51
NM_003124SPR1
NM_023947CHID11
NM_006727CDH101
NM_005338HIP11
NM_004755RPS6KA51
NM_138290RPIB91
NM_000321RB11
NM_015318ARHGEF181
NM_013261PPARGC1A1
NM_058166TRIM61
NM_001003818TRIM61
NM_001077511TCF191
NM_007109TCF191
NM_005075SLCO1A21
NM_001039693FLJ379531
XM_934517LOC6472121
NM_175910ZNF4931
NM_001076678ZNF4931
NM_001011720XKR91
NM_020675SPC251
NM_002547OPHN11
NM_001011552SLC9A41
NM_0146951
NM_013937OR11A11
NM_018100EFHC11
NM_173851SLC30A81
NM_014895KIAA10091
NM_005840SPRY31
NM_013943CLIC41
NM_020905RDH141
NM_145055C18orf251
NM_001008239C18orf251
NM_175883OR7D21
NM_024953C12orf301
NM_033058TRIM551
NM_184085TRIM551
NM_184086TRIM551
NM_184087TRIM551
NM_017633FAM46A1
NM_001039199C20orf1211
NM_024331C20orf1211
XM_001130815LOC6478591
XM_001127102LOC6478591
NM_005869SDCCAG101
NM_014358CLEC4E1
NM_000038APC1
NM_007131ZNF751
NM_014616ATP11B1
NM_001013717LOC4411081
NM_152626ZNF921
NM_007139ZNF921
NM_024416OGN1
NM_014057OGN1
NM_033014OGN1
NM_004797ADIPOQ1
NM_018238AGK1
NM_0207221
NM_001873CPE1
NM_000670ADH41
NM_000995RPL341
NM_033625RPL341
NM_182767SLC6A151
NM_153810C10orf461
NM_003800RNGTT1
NM_017919STX171
NM_025188TRIM451
NM_0056401
NM_005748YAF21
NM_198935SS18L11
NM_014269ADAM291
XM_926071LOC6425921
NM_0010996921
NM_033381COL4A51
NM_033380COL4A51
NM_000495COL4A51
NM_032859ABHD131
NM_000081LYST1
NM_020139BDH21
NM_002936RNASEH11
NM_201648GLYAT1
NM_032560SMEK11
NM_022041GAN1
NM_033051SLC46A21
NM_033285TP53INP11
NM_133496SLC30A71
NM_022346NCAPG1
NM_016275SELT1
NM_023016ANKRD571
NM_080927DCBLD21
NM_001011538LOC4021761
NM_017705PAQR51
NM_0011045541
NM_014864FAM20B1
NM_138444KCTD121
NM_015130TBC1D91
NM_025228TRAF3IP31
NM_153216FLJ256801
NM_032440LCOR1
NM_015642ZBTB201
NM_152622MIER31
NM_001013655LOC3898341
XM_001125792LOC7278341
XM_926013LOC6423981
NM_018948ERRFI11
NM_148886SMCR71
NM_139162SMCR71
XM_001132872LOC7286771
NM_014631SH3PXD2A1
NM_173822FAM126B1
NM_1989451
NM_0010992731
NM_000233LHCGR1
NM_014410CLUL11
NM_199167CLUL11
NM_020800IFT801
NM_182904P4HA31
NM_003045SLC7A11
NM_015553PIP3-E1
XM_496115LOC4403381
XM_940659LOC4403381
NM_032622LNX11
NM_214711LOC4011371
NM_012297G3BP21
NM_203504G3BP21
NM_203505G3BP21
NM_020718USP311
NM_032961PCDH101
NM_018366CNO1
NM_003094SNRPE1
NM_018373SYNJ2BP1
NM_0011013961
NM_007212RNF21
NM_020546ADCY21
NM_139281WDR361
NM_018182C17orf631
NM_001077498C17orf631
NM_015253WSCD11
NM_001033659FLJ358481
NM_020933ZNF3171
NM_052885SLC2A131
NM_0010982721
NM_002130HMGCS11
NM_032826SLC35B41
XM_376795FLJ458721
XM_940037FLJ458721
NM_002449MSX21
NM_020944GBA21
NM_015644TTLL31
NM_031284ADPGK1
NM_172365C14orf501
NM_182580CYB561D11
NM_024631C11orf611
NM_021116ADCY11
NM_0153721
NM_178863KCTD131
NM_138340ABHD31
NM_016438HIGD1B1
NM_031476CRISPLD21
NM_003559PIP5K2B1
NM_001329CTBP21
NM_0010839141
NM_022802CTBP21
NM_014044UNC501
NM_145168HSPC1051
NM_013438UBQLN11
NM_002318LOXL21
NM_198562C3orf621
NM_001039570KREMEN11
NM_001039571KREMEN11
NM_033339CASP71
NM_001227CASP71
NM_033340CASP71
NM_033338CASP71
NM_002942ROBO21
NM_016621PHF21A1
NM_0011018021
NM_0010839621
NM_003199TCF41
NM_002958RYK1
NM_001005861RYK1
NM_015149RGL11
NM_001742CALCR1
XM_375929LOC4008561
NM_031922REPS11
NM_138348FAM105B1
NM_014766SCRN11
NM_001656TRIM231
NM_020208SLC6A201
NM_022405SLC6A201
NM_178136POLDIP31
NM_032311POLDIP31
NM_022894PAPOLG1
NM_014819PJA21
NM_001013640LOC3885031
NM_021970MAP2K1IP11
NM_005986SOX11
NM_005436CCDC61
NM_0209101
NM_016604JMJD1B1
NM_145693LPIN11
NM_005399PRKAB21
XM_001125704LOC7277781
NM_145257C1orf961
NM_024104C19orf421
NM_032521PARD6B1
NM_001931DLAT1
NM_004930CAPZB1
NM_032926TCEAL31
NM_001006933TCEAL31
NM_001012979TCEAL51
NM_0147221
NM_003017SFRS31
NM_144571CNOT6L1
NM_145284FAM122B1
NM_020960GPR1071
NM_016046EXOSC11
XM_001132419SLC35F31
NM_173508SLC35F31
NM_003406YWHAZ1
NM_145690YWHAZ1
NM_033225CSMD11
NM_000921PDE3A1
XM_001125711LOC7304531
NM_018941CLN81
XM_931376SETD51
XM_944260SETD51
XM_931478SETD51
XM_944280SETD51
XM_931417SETD51
XM_944276SETD51
XM_939712SETD51
NM_001080517SETD51
XM_944303SETD51
XM_931455SETD51
XM_931447SETD51
XM_944298SETD51
XM_944295SETD51
XM_931444SETD51
NM_153487MDGA11
NM_002657PLAGL21
NM_030626LRRC271
NM_0010996911
NM_003236TGFA1
NM_004879EI241
NM_001007277EI241
NM_1829691
NM_175911OR2L131
NM_001042492NF11
NM_000267NF11
NM_001483GBAS1
NM_019018FAM105A1
NM_138292ATM1
NM_000051ATM1
NM_016053CCDC531
XM_926086LOC6531141
NM_004612TGFBR11
NM_030581WDR591
NM_002740PRKCI1
NM_006226PLCL11
NM_025112ZXDC1
NM_017623CNNM31
NM_199078CNNM31
NM_015153PHF31
NM_005375MYB1
NM_007214SEC631
NM_003108SOX111
NM_181581DUS4L1
NM_001001875TPD52L31
NM_033516TPD52L31
NM_001078173FAM127C1
NM_001024843TNRC6B1
NM_015088TNRC6B1
NM_004176SREBF11
NM_001005291SREBF11
NM_015609C1orf1441
NM_013236ATXN101
NM_016353ZDHHC21
NM_021943ZFAND31
NM_024837ATP8B41
NM_015111N4BP31
NM_001552IGFBP41
NM_016467ORMDL11
NM_001627ALCAM1
NM_016300ARPP-211
NM_020125SLAMF81
NM_198859PRICKLE21
NM_016337EVL1
NM_004964HDAC11
NM_002703PPAT1
NM_006371CRTAP1
NM_032632PAPOLA1
NM_0011101991
NM_012453TBL21
NM_033544RCCD11
NM_001017919RCCD11
XM_935586LOC6418451
XM_938850LOC6470871
XM_930101LOC6470871
NM_019083CCDC761
NM_016513ICK1
NM_014920ICK1
NM_001080494C1orf341
XM_934658C1orf341
XM_945965C1orf341
XM_942427C1orf341
XM_930328C1orf341
XM_945959C1orf341
NM_015047KIAA00901
NM_021145DMTF11
NM_194329RNF381
NM_194328RNF381
NM_194330RNF381
NM_022781RNF381
NM_194332RNF381
NM_005604POU3F21
NM_005195CEBPD1
NM_199040NUDT41
NM_019094NUDT41
NM_004808NMT21
NM_001083112GPD21
NM_000408GPD21
NM_002429MMP191
XM_001133377LOC7324151
NM_080650ATPBD41
NM_152332MTAC2D11
NM_003281TNNI11
NM_000728CALCB1
XM_939186GPSM11
NM_006947SRP721
NM_006108SPON11
NM_004206SEC22C1
NM_018650MARK11
NM_001008226DKFZp666G0571
NM_021783EDA2R1
NM_004535MYT11
NM_201267NRP21
NM_018534NRP21
NM_152564VPS13B1
NM_017890VPS13B1
NM_003374VDAC11
NM_198461LONRF21
NM_012249RHOQ1
NM_198274SMYD11
NM_016040TMED51
NM_000838GRM11
NM_001608ACADL1
NM_152874FAS1
NM_152876FAS1
NM_152877FAS1
NM_152872FAS1
NM_152871FAS1
NM_152873FAS1
NM_000043FAS1
NM_152875FAS1
NM_024665TBL1XR11
NM_024558C14orf1381
NM_001040662C14orf1381
NM_006056NMUR11
NM_006439MAB21L21
XM_001130278MAB21L21
NM_003106SOX21
XM_942780SYNPO21
NM_181443BTBD31
NM_014962BTBD31
NM_203284RBPJ1
NM_203283RBPJ1
NM_015874RBPJ1
NM_005349RBPJ1
NM_016315GULP11
NM_0151311
NM_014553TFCP2L11
NM_022845CBFB1
NM_001755CBFB1
NM_207321C10orf1291
XM_001127274SMYD21
NM_022755IPPK1
NM_030911CDADC11
NM_033656BRWD11
NM_004857AKAP51
NM_003304TRPC11
NM_152263TPM31
NM_002508NID11
NM_001080530LOC920171
NM_080743SRrp351
NM_031949TTLL21
NM_080616C20orf1121
NM_016252BIRC61
NM_001031683IFIT31
NM_001549IFIT31
NM_002655PLAG11
NM_152998EZH21
NM_004456EZH21
NM_006661PDE10A1
NM_203364CAPRIN11
NM_004776B4GALT51
NM_006204PDE6C1
NM_003922HERC11
NM_015569DNM31
NM_0010994031
NM_020226PRDM81
NM_000313PROS11
NM_001025436SPAG161
NM_018017C10orf1181
NM_006866LILRA21

Immunostimulatory motif search results


UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).

GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).



Settings used for search

Scores were calculated using scoring scheme SS1
Using AVG scoring mode.

Hit scores

ParameterSpec. Score cut-offColourComment
Hit to same geneColourHits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit.
High scoring hit≥80ColourHits are very likely to cause an off-target effect
Medium scoring hit<80¸≥40ColourHits with a small chance of causing an off target effect.
Low scoring hitColourHits very unlikely to cause an off-target effect

The mismatches are colour coded based on the weighting of that position.

Cut-offColour
MatchColour
Wobble base pairs (G-U)Colour
Critical bases (none)Colour
0.6 bitsColour
0.4 bitsColour
0.2 bitsColour

About SpecificityServer

SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).

Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer


For more details about the algorithm "siRNA specificity searching incorporating mismatch tolerance data" Bioinformatics (2008) 24 (10): 1316-1317