Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands
13 hits were found for CGUCCCCUCCGGCCGUACC (1 guide strand hits and 12 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_020184 | CNNM4 | 2036 | 2054 | g | G | U | C | C | C | C | U | C | C | G | a | C | C | G | U | u | C | C | 40 | 3 | 10 | false | <90% | 4814 | |||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_001703 | BAI2 | 4479 | 4497 | G | G | c | c | C | G | G | C | C | G | G | A | G | G | G | G | A | C | c | 38 | 3 | 14 | bc | false | <90% | 5434 | ||
| NM_017978 | ANKHD1 | 324 | 342 | G | G | U | A | C | G | G | g | C | G | G | A | G | G | G | G | A | C | G | 37 | 1 | 11 | bc | false | <90% | 2149 | ||
| NM_024668 | ANKHD1 | 324 | 342 | G | G | U | A | C | G | G | g | C | G | G | A | G | G | G | G | A | C | G | 37 | 1 | 11 | bc | false | <90% | 2164 | ||
| NM_017747 | ANKHD1 | 324 | 342 | G | G | U | A | C | G | G | g | C | G | G | A | G | G | G | G | A | C | G | 37 | 1 | 11 | bc | false | <90% | 8196 | ||
| NM_020690 | MASK-BP3 | 324 | 342 | G | G | U | A | C | G | G | g | C | G | G | A | G | G | G | G | A | C | G | 37 | 1 | 11 | bc | false | <90% | 8319 | ||
| XM_001129427 | LOC649768 | 7035 | 7053 | c | G | U | g | C | G | G | C | C | G | G | A | G | G | G | G | c | C | G | 37 | 3 | 12 | c | false | <90% | 16186 | ||
| XM_001129427 | LOC649768 | 9135 | 9153 | c | G | U | g | C | G | G | C | C | G | G | A | G | G | G | G | c | C | G | 37 | 3 | 12 | c | false | <90% | 16186 | ||
| XM_001129427 | LOC649768 | 10806 | 10824 | c | G | U | g | C | G | G | C | C | G | G | A | G | G | G | G | c | C | G | 37 | 3 | 12 | c | false | <90% | 16186 | ||
| XM_001126093 | LOC727897 | 7044 | 7062 | c | G | U | g | C | G | G | C | C | G | G | A | G | G | G | G | c | C | G | 37 | 3 | 12 | c | false | <90% | 17695 | ||
| XM_001126093 | LOC727897 | 8628 | 8646 | c | G | U | g | C | G | G | C | C | G | G | A | G | G | G | G | c | C | G | 37 | 3 | 12 | c | false | <90% | 17695 | ||
| XM_001126093 | LOC727897 | 12315 | 12333 | c | G | U | g | C | G | G | C | C | G | G | A | G | G | G | G | c | C | G | 37 | 3 | 12 | c | false | <90% | 17695 | ||
| NM_017556 | FBLIM1 | 11 | 29 | x | G | g | A | C | G | G | g | C | G | G | A | G | G | G | G | A | C | G | 16 | 3 | 11 | bc | false | <90% | 3363 | ||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 842
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer