Predicted Off-targets for siRNA "cucguccccuccggccguacc" Using siRNA SpecificityServer

Specificity Server [Result page v1.0]


SpecificityServer - An siRNA design program for determining siRNA specificity

Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands

13 hits were found for CGUCCCCUCCGGCCGUACC (1 guide strand hits and 12 sense strand hits.)

Hits to Guide strand

AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
NM_020184 CNNM4 2036 2054 gGUCCCCUCCGaCCGUuCC40 3 10   false   <90%   4814


Hits to Sense strand













AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
NM_001703 BAI2 4479 4497 GGccCGGCCGGAGGGGACc38 3 14 bc false   <90%   5434
NM_017978 ANKHD1 324 342 GGUACGGgCGGAGGGGACG37 1 11 bc false   <90%   2149
NM_024668 ANKHD1 324 342 GGUACGGgCGGAGGGGACG37 1 11 bc false   <90%   2164
NM_017747 ANKHD1 324 342 GGUACGGgCGGAGGGGACG37 1 11 bc false   <90%   8196
NM_020690 MASK-BP3 324 342 GGUACGGgCGGAGGGGACG37 1 11 bc false   <90%   8319
XM_001129427 LOC649768 7035 7053 cGUgCGGCCGGAGGGGcCG37 3 12 c false   <90%   16186
XM_001129427 LOC649768 9135 9153 cGUgCGGCCGGAGGGGcCG37 3 12 c false   <90%   16186
XM_001129427 LOC649768 10806 10824 cGUgCGGCCGGAGGGGcCG37 3 12 c false   <90%   16186
XM_001126093 LOC727897 7044 7062 cGUgCGGCCGGAGGGGcCG37 3 12 c false   <90%   17695
XM_001126093 LOC727897 8628 8646 cGUgCGGCCGGAGGGGcCG37 3 12 c false   <90%   17695
XM_001126093 LOC727897 12315 12333 cGUgCGGCCGGAGGGGcCG37 3 12 c false   <90%   17695
NM_017556 FBLIM1 11 29 xGgACGGgCGGAGGGGACG16 3 11 bc false   <90%   3363


3 prime UTR Seed matches

Hits to same gene : 0
Hits to other genes : 842
View matched accessions

3 prime seed match details

AccessionGene nameHits to this gene
NM_022158FN3K17
NM_032189ATP11A10
NM_015205ATP11A10
NM_199454PRDM165
NM_022114PRDM165
NM_015227POFUT23
NM_001077238SPPL2B3
XM_001126137LOC2849523
XM_001129340LOC2849523
NM_013435RAX3
NM_002501NFIX3
NM_207313TMEM132E3
NM_177996EPB41L12
NM_012156EPB41L12
NM_015557CHD52
NM_182557BCL9L2
NM_0152972
XM_379029LOC4008792
NM_032843FIBCD12
NM_052899GPRIN12
NM_152988SPPL2B2
NM_199141CARM12
NM_020469ABO2
XM_001131408LOC7303502
XM_001127666LOC7308962
NM_015321CRTC12
NM_0010984822
NM_003926MBD32
NM_052909KIAA19092
NM_015493ANKRD252
XM_943278LOC6447272
XM_932334LOC6447272
NM_001037165FOXK12
NM_024845FLJ141542
NM_001083600FLJ141542
NM_001083601FLJ141542
NM_016155MMP172
XM_001133320MMP172
NM_001042486DLGAP42
NM_014902DLGAP42
NM_183006DLGAP42
NM_001007139IGF22
NM_000612IGF22
NM_020708SLC12A52
NM_015094HIC22
NM_183397PXMP42
NM_007238PXMP42
NM_144564SLC39A32
NM_014079KLF152
NM_012186FOXE32
XM_001132770FOXE32
NM_032261C21orf562
NM_001194HCN22
NM_020132AGPAT32
NM_001037553AGPAT32
NM_020170NCLN2
NM_005990STK102
NM_138356SHF2
NM_005852CHD32
NM_001005271CHD32
NM_001005273CHD32
XM_928866LOC6458952
XM_938922LOC6458952
NM_020713ZNF512B2
NM_005740DNAL42
NM_153609TMPRSS62
XM_936831IQSEC32
NM_003504CDC45L1
NM_080664C14orf1261
NM_203451LOC4001201
NM_130440PTPRF1
NM_002840PTPRF1
NM_001817CEACAM41
NM_014080DUOX21
NM_001004019FBLN21
NM_001998FBLN21
NM_006742PSKH11
NM_001264CDSN1
NM_001024227ARF11
NM_001658ARF11
NM_001024228ARF11
NM_001024226ARF11
NM_003088FSCN11
NM_032430BRSK11
NM_001012644DUSP151
NM_177991DUSP151
NM_080611DUSP151
NM_020851ISLR21
NM_000836GRIN2D1
NM_144599NIPA11
NM_025225PNPLA31
NM_022779DDX311
NM_207391RGS9BP1
NM_022460HS1BP31
NM_003307TRPM21
NM_020448NPAL31
NM_007014WWP21
NM_199424WWP21
NM_019089HES21
NM_001433ERN11
NM_021641ADAM121
NM_001628AKR1B11
NM_0011008751
NM_152598RNF1901
NM_018992KCTD51
NM_182563C16orf791
NM_020126SPHK21
NM_001949E2F31
NM_000626CD79B1
NM_001039933CD79B1
NM_021602CD79B1
NM_001846COL4A21
NM_003121SPIB1
NM_152253CHKB1
NM_152247CPT1B1
NM_004377CPT1B1
NM_152245CPT1B1
NM_032753RAXL11
NM_017955CDCA41
NM_003417ZNF2641
NM_173476CCDC781
NM_005036PPARA1
NM_001001928PPARA1
NM_017859UCKL11
NM_052847GNG71
NM_017931FLJ206991
NM_013397C6orf491
NM_020956PRX1
NM_013385PSCD41
NM_002883RANGAP11
NM_145714ATXN2L1
NM_007245ATXN2L1
NM_148415ATXN2L1
NM_148416ATXN2L1
NM_148414ATXN2L1
NM_0010987901
NM_021242MID1IP11
NM_0010987911
NM_002885RAP1GAP1
NM_203343EPB411
NM_203342EPB411
NM_004437EPB411
NM_017585SLC2A61
XM_001134396LOC7302541
NM_0153021
NM_014238KSR11
NM_020372SLC22A171
NM_016609SLC22A171
NM_001478B4GALNT11
NM_023947CHID11
NM_015063SLC8A21
NM_018670MESP11
NM_005628SLC1A51
NM_002502NFKB21
NM_001077494NFKB21
NM_001077493NFKB21
NM_021926ALX41
NM_002859PXN1
NM_1522931
NM_014460CSDC21
NM_015042ZNF6091
NM_019096GTPBP21
NM_006485FBLN11
NM_003707RUVBL11
NM_145279MOBKL2C1
NM_201403MOBKL2C1
NM_017770ELOVL21
NM_001083589E2F51
NM_001083588E2F51
NM_001951E2F51
NM_145899HMGA11
NM_145901HMGA11
NM_002131HMGA11
NM_145903HMGA11
NM_145902HMGA11
NM_145904HMGA11
NM_145905HMGA11
NM_003681PDXK1
NM_198236ARHGEF111
NM_014784ARHGEF111
NM_022648TNS11
NM_024958NRSN21
NM_021012KCNJ121
NM_001083591CUGBP21
NM_001025077CUGBP21
NM_006561CUGBP21
NM_001025076CUGBP21
NM_020820PREX11
NM_138440VASN1
NM_002084GPX31
NM_033223GABRG31
XM_943034LOC6430851
XM_931304LOC6430851
NM_001419ELAVL11
NM_015193ARC1
NM_144626TMEM1251
NM_005920MEF2D1
NM_002638PI31
NM_003862FGF181
NM_002579PALM1
NM_001040134PALM1
NM_145175FAM84A1
NM_148960CLDN191
NM_139159DPP91
NM_003225TFF11
NM_012145DTYMK1
XM_001126184LOC7277611
XM_001126211LOC7277611
XM_001126163LOC7277611
XM_001126193LOC7277611
NM_018043TMEM16A1
XM_001131760LOC7286761
XM_001131632LOC7310111
NM_019020TBC1D161
XM_001125711LOC7304531
NM_031475ESPN1
NM_012306FAIM21
NM_002676PMM11
NM_058238WNT7B1
NM_183376ARRDC41
NM_006936SUMO31
NM_015686TMEM281
NM_194460RNF1261
NM_013313YPEL11
NM_014562OTX11
NM_024735FBXO311
NM_014468VENTX1
NM_175609ARFGAP11
NM_018209ARFGAP11
NM_016337EVL1
NM_004204PIGQ1
XM_001131399LOC7319621
XM_001131436LOC7298221
NM_003486SLC7A51
NM_006897HOXC91
NM_0011099771
NM_181709FAM101A1
NM_152495CNIH31
NM_021962ABR1
NM_001092ABR1
NM_001006667TAC31
NM_032451SPIRE21
NM_001024858SPTB1
NM_178122C1orf2011
NM_004227PSCD31
NM_002911UPF11
NM_012305AP2A21
NM_032482DOT1L1
NM_014577BRD11
NM_002725PRELP1
NM_201348PRELP1
NM_024313NOL121
NM_001039360ZBTB7C1
NM_014726TBKBP11
NM_171825CAMK2A1
NM_015981CAMK2A1
NM_007174CIT1
NM_0010992941
XM_936510KIAA16441
NM_133369UNC5A1
NM_015274MAN2B21
NM_021267LASS11
NM_000832GRIN11
NM_007327GRIN11
NM_021569GRIN11
NM_020822KCNT11
NM_000883IMPDH11
NM_0011026051
NM_183243IMPDH11
NM_052897MBD61
NM_198946LCN61
NM_000453SLC5A51
NM_033224PURB1
NM_030581WDR591
NM_032193RNASEH2C1
NM_001678ATP1B21
NM_052845MMAB1
NM_144775SMCR81
NM_001945HBEGF1
NM_032595PPP1R9B1
XM_001134027LOC7318891
XM_001134374LOC7301321
NM_017980LIMS21
NM_001707BCL7B1
NM_006114TOMM401
NM_002309LIF1
NM_178449TIP391
NM_176677FLJ362081
NM_018373SYNJ2BP1
NM_003274TMEM11
NM_006510TRIM271
NM_006336ZER11
NM_012121CDC42EP41
NM_0149871
NM_014897ZNF6521
NM_001219CALU1
NM_019044CCDC931
NM_0011102201
NM_0011102211
NM_006783GJB61
NM_0011102191
NM_001039577CCNL21
NM_005596NFIB1
NM_032420PCDH11
NM_000368TSC11
NM_005417SRC1
NM_198291SRC1
XM_001130189LOC7315981
XM_001129850LOC7292911
XM_001132325LOC7300811
XM_001127777LOC7309341
NM_021949ATP2B31
NM_006093PRG31
NM_003036SKI1
NM_001018000KIAA10261
NM_001017999KIAA10261
NM_001018001KIAA10261
NM_015209KIAA10261
NM_001078172FAM127B1
NM_199054MKNK21
XM_001133925GRIP11
XM_001133901GRIP11
XM_001133924GRIP11
XM_001133902GRIP11
NM_182729TXNRD11
NM_0010937711
NM_182742TXNRD11
NM_003330TXNRD11
NM_182743TXNRD11
XM_001132308LOC7310861
XM_001130384LOC6441661
XM_496991LOC4413771
XM_938599LOC4413771
NM_000767CYP2B61
NM_024676C1orf1131
NM_032039ITFG31
NM_015073SIPA1L31
NM_002181IHH1
NM_052839PANX21
NM_138736GNAO11
NM_023072ZSWIM41
NM_178832C10orf831
NM_0010988311
NM_138465GLI41
XM_942210LOC6505621
XM_173132LOC2556201
NM_177405CECR11
NM_017424CECR11
NM_004983KCNJ91
NM_022117TSPYL21
XM_001128695TSPYL21
NM_002414CD991
NM_178129P2RY81
NM_022062PKNOX21
NM_001001694FLJ419931
NM_016734PAX51
NM_004502HOXB71
NM_003978PSTPIP11
NM_173578C11orf721
NM_005456MAPK8IP11
NM_138774C19orf221
NM_003192TBCC1
XM_001124643LOC7304161
NM_152296ATP1A31
NM_178003PPP2R41
NM_021131PPP2R41
NM_178000PPP2R41
NM_178001PPP2R41
NM_032167RUNDC2A1
NM_019594LRRC8A1
NM_198695KRTAP10-81
NM_178865SERINC21
NM_001039199C20orf1211
NM_024331C20orf1211
NM_003227TFR21
NM_003565ULK11
XM_001133335ULK11
NM_016376ANKFY11
NM_138471LOC1440971
NM_005883APC21
NM_152699SENP51
NM_0011052821
NM_001631ALPI1
NM_002504NFX11
NM_031945TSPAN101
NM_016263FZR11
NM_003846PEX11B1
NM_004296RGS61
NM_014228SLC6A71
NM_007032TRIOBP1
NM_001039141TRIOBP1
NM_024103SLC25A231
NM_024565CCNJL1
NM_020526EPHA81
NM_002777PRTN31
NM_014434NDOR11
NM_173344ST3GAL11
NM_003033ST3GAL11
NM_015264C22orf91
NM_001009880C22orf91
NM_017765PQLC21
NM_001040126PQLC21
NM_001040125PQLC21
NM_001002862DERL31
NM_198440DERL31
NM_001001685FLJ450791
XM_934676LOC6449251
XM_942888LOC6449251
NM_002473MYH91
NM_000599IGFBP51
NM_006640SEPT91
NM_005318H1F01
NM_003200TCF31
NM_030629CMIP1
NM_198390CMIP1
XM_940825DLGAP31
NM_001080418DLGAP31
NM_032809FAM73B1
NM_024086METT10D1
NM_173156SMG71
NM_201569SMG71
NM_201568SMG71
NM_020452ATP8B21
NM_178448C9orf1401
NM_0010988331
NM_0202181
NM_003685KHSRP1
NM_175910ZNF4931
NM_001076678ZNF4931
NM_080836STK351
NM_014286FREQ1
NM_178493NOTUM1
NM_014746RNF1441
NM_145185MAP2K71
NM_033129SCRT21
NM_015898ZBTB7A1
NM_006927ST3GAL21
NM_015949C7orf201
NM_015099CAMTA21
NM_021574BCR1
NM_004327BCR1
XM_933701LOC6441651
NM_001001331ATP2B21
NM_001683ATP2B21
NM_0010096101
NM_173482CCDC1051
NM_207379TMEM1791
NM_001001795MGC708571
NM_017806LIME11
NM_0010994561
NM_199138FAM123A1
NM_152704FAM123A1
NM_024016HOXB81
NM_001042782TNNT31
NM_001042780TNNT31
NM_001042781TNNT31
NM_006757TNNT31
NM_198586NHLRC11
NM_002339LSP11
NM_001013255LSP11
NM_001013253LSP11
NM_001013254LSP11
NM_015011MYO161
NM_005892FMNL11
NM_0011006261
NM_006459ERLIN11
NM_015831ACHE1
NM_000665ACHE1
XM_001128998BRUNOL41
NM_020180BRUNOL41
NM_139062CSNK1D1
NM_001893CSNK1D1
XM_937998LOC1238551
XM_063871LOC1238551
NM_198686RAB151
NM_001001414LOC3428971
NM_015908ARS21
NM_182800ARS21
NM_004302ACVR1B1
NM_138795ARL8A1
NM_001294CLPTM11
NM_001009934DNASE1L11
NM_001009932DNASE1L11
NM_006730DNASE1L11
NM_001009933DNASE1L11
NM_005654NR2F11
NM_005197CHES11
NM_0010854711
NM_001012508SPRN1
NM_004730ETF11
NM_002541OGDH1
NM_015470RAB11FIP51
NM_0010937251
NM_138383ABBA-11
NM_005161AGTRL11
NM_003131SRF1
NM_152222TNFRSF19L1
NM_032871TNFRSF19L1
NM_133633SYN31
NM_003490SYN31
NM_198595AFAP11
NM_021638AFAP11
NM_152694ZCCHC51
NM_001276CHI3L11
NM_152307C14orf1721
NM_002442MSI11
NM_020795NLGN21
NM_025058TRIM461
NM_005194CEBPB1
XM_933066LOC6436961
XM_001130526LOC7286361
XM_001130555LOC7286361
XM_001130543LOC7286361
XM_001130536LOC7286361
XM_001129399LOC6423461
XM_928707LOC6423461
XM_925879LOC6423461
NM_0010853751
NM_001347DGKQ1
NM_022006FXYD71
NM_024811FLJ125291
NM_001029880SFMBT21
NM_033061KRTAP4-71
NM_002360MAFK1
NM_152739HOXA91
NM_015288PHF151
NM_007028TRIM311
NM_199131VAX11
NM_005861STUB11
NM_199421SOCS41
NM_080867SOCS41
NM_016545IER51
XM_001132246LOC7299971
NM_016433GLTP1
NM_031431COG31
NM_172390NFATC11
NM_000411HLCS1
NM_016511CLEC1A1
NM_0156561
NM_052874STX1B21
NM_015155LARP51
NM_015318ARHGEF181
NM_020309SLC17A71
NM_022834VWA11
NM_199121VWA11
NM_0011110191
NM_145117NAV21
NM_182964NAV21
NM_0011110181
NM_001009998SSBP41
NM_032627SSBP41
NM_002972SBF11
NM_004603STX1A1
NM_130446KLHL61
NM_020831MKL11
NM_001013690LOC4017201
NM_002653PITX11
NM_032603LOXL31
NM_001715BLK1
NM_001328CTBP11
NM_001012614CTBP11
NM_004174SLC9A31
NM_201520SLC25A351
NM_020335VANGL21
NM_003108SOX111
NM_033446FAM125B1
NM_199169TMEPAI1
NM_199170TMEPAI1
NM_199171TMEPAI1
NM_020182TMEPAI1
NM_025248SNIP1
NM_145245EVI5L1
NM_173832ZFP411
NM_048368CTDP11
NM_004715CTDP11
XM_001131308LOC7309611
NM_181491SURF51
NM_014275MGAT4B1
NM_054013MGAT4B1
NM_016479SCOTIN1
NM_178565RSPO21
XM_940609FLJ250761
XM_059689FLJ250761
NM_199046TEPP1
NM_199456TEPP1
NM_014190ADD11
NM_001119ADD11
NM_014189ADD11
NM_176801ADD11
NM_000033ABCD11
NM_001024401SBK11
NM_0010994091
NM_000841GRM41
NM_052906LRRC621
NM_080430SELM1
NM_004561OVOL11
XM_001129344OVOL11
XM_001131395LOC7298101
XM_001126629LOC7306341
NM_001080453INTS11
XM_935548INTS11
XM_942544INTS11
NM_032028TSSK1B1
NM_018316KLHL261
NM_024722ACBD41
NM_003580NSMAF1
NM_015260SIN3B1
NM_025251KIAA16881
NM_003378VGF1
NM_019613WDR45L1
NM_000278PAX21
NM_003987PAX21
NM_003988PAX21
NM_022829SLC13A31
NM_001011554SLC13A31
NM_198492CLEC4G1
NM_024861FLJ226711
NM_0010854371
NM_017575SMG61
NM_182925FLT41
NM_018378FBXL81
NM_006077CBARA11
NM_033100PCDH211
NM_012398PIP5K1C1
NM_006160NEUROD21
NM_005928MFGE81
NM_001080396LOC7282151
XM_001133620LOC7318951
NM_001387DPYSL31
NM_014770CENTG11
NM_005018PDCD11
NM_005370RAB8A1
NM_031448C19orf121
NM_001031726C19orf121
NM_015995KLF131
NM_194071CREB3L21
NM_024589ROGDI1
NM_012081ELL21
NM_015409EP4001
NM_002401MAP3K31
NM_203351MAP3K31
NM_001077620PRCD1
NM_0010984981
NM_0010984971
NM_001039948RUTBC21
NM_133454RUTBC21
NM_030792GDPD51
XM_036936LOC4408041
NM_0156721
NM_014634PPM1F1
NM_003030SHOX21
NM_006884SHOX21
NM_016458C8orf30A1
XM_001126758LOC7280711
NM_006504PTPRE1
NM_130435PTPRE1
NM_178273PILRA1
NM_002247KCNMA11
NM_182894CHX101
NM_022730COPS7B1
NM_007011ABHD21
NM_152924ABHD21
NM_031417MARK41
NM_0011051931
NM_0011051951
NM_0011051941
NM_152698FLJ383771
NM_025243SLC19A31
NM_001017395TMCC11
NM_015008TMCC11
NM_152757FLJ303131
NM_001045478LOC6449971
NM_182507KRT801
NM_001081492KRT801
NM_207582HERV-FRD1
NM_033274ADAM191
NM_022124CDH231
NM_005938MLLT71
NM_152470RNF1651
NM_022078GPATCH31
NM_031283TCF7L11
XM_943686SYT71
NM_001520GTF3C11
NM_152744SDK11
NM_153490KRT131
NM_002274KRT131
NM_021175HAMP1
NM_000332ATXN11
NM_002739PRKCG1
XM_001125933LOC7277371
NM_002602PDE6G1
NM_015711GLTSCR11
NM_001039656MTL51
NM_004248PRLHR1
NM_139018CD300LF1
NM_145685BRF11
NM_001519BRF11
NM_001042472ABHD121
NM_182970RIMS41
XM_001132928LOC7289691
NM_004807HS6ST11
NM_015537NELF1
NM_007326CYB5R31
NM_000398CYB5R31
NM_000727CACNG11
NM_016352CPA41
NM_006553SLMO11
NM_001008223C1QL41
NM_014974DIP2C1
NM_020698TMCC31
NM_012239SIRT31
NM_001017524SIRT31
NM_012140SLC25A101
NM_006732FOSB1
NM_024681KCTD171
NM_001002901FCRLB1
NM_006775QKI1
NM_002855PVRL11
NM_145068TRPV31
XM_001129558LOC7291751
XM_001131204LOC7319031
NM_013238DNAJC151
NM_006482DYRK21
NM_003583DYRK21
NM_012292HMHA11
NM_020310MNT1
NM_031910C1QTNF61
NM_021135RPS6KA21
NM_001006932RPS6KA21
NM_023018NADK1
NM_006598SLC12A71
NM_000566FCGR1A1
NM_024816RABEP21
NM_000120EPHX11
NM_023078PYCRL1
NM_002229JUNB1
NM_002688SEPT51
NM_022764MTHFSD1
NM_025163PIGZ1
NM_004077CS1
NM_198324CS1
NM_002886RAP2B1
NM_032382COG81
NM_001040701FUT61
NM_000150FUT61
NM_001082575HRNBP31
NM_152221CSNK1E1
NM_001894CSNK1E1
NM_001552IGFBP41
NM_033542SYS11
NM_001116ADCY91
NM_005867DSCR41
NM_032808LINGO11
NM_006387CHERP1
NM_007203PALM2-AKAP21
NM_147150PALM2-AKAP21
NM_001004065AKAP21
XM_001132993LOC7302181
XM_001132827LOC7323291
XM_498907LOC2849981
XM_944715LOC2849981
NM_178818CMTM41
NM_181521CMTM41
NM_0011052471
NM_024742ARMC51
NM_006668CYP46A11
NM_145762GFRA41
NM_022139GFRA41
NM_021649TICAM21
NM_020689SLC24A31
NM_004321KIF1A1
XM_001133198LOC6444101
NM_000831GRIK31
NM_000486AQP21
NM_001040628NCALD1
NM_001040626NCALD1
NM_001040625NCALD1
NM_032041NCALD1
NM_001040629NCALD1
NM_001040624NCALD1
NM_001040627NCALD1
NM_001040630NCALD1
NM_024704C20orf231
NM_015174KIAA10861
XM_001128953LOC7312491
XM_001127675LOC7285301
NM_017584MIOX1
NM_000391TPP11
NM_022003FXYD61
NM_001001549GRB101
NM_001001555GRB101
NM_001001550GRB101
NM_005311GRB101
NM_175066DDX511
NM_174917LOC1973221
NM_152600ZNF5791
NM_001039574KCNC41
NM_004978KCNC41
NM_022166XYLT11
XM_001125986LOC2838241
NM_016046EXOSC11
NM_031215CABLES21
NM_001451FOXF11
NM_001080506FLJ129931
XM_001128552FLJ129931
NM_018028SAMD4B1
NM_001895CSNK2A11
NM_177560CSNK2A11
NM_177559CSNK2A11
NM_152914MGC338941
NM_001082959SCARB11
NM_005505SCARB11
NM_006176NRGN1
NM_006819STIP11
NM_014766SCRN11
NM_014994MAPKBP11
NM_000774CYP2F11
XM_001131525LOC7319921
XM_927936LOC6448411
XM_935685LOC6419331
XM_938176LOC6448411
NM_013411AK21
NM_015161ARL6IP11
XM_927238LOC6439881
XM_936418LOC6473471
XM_930750LOC6423661
XM_943652LOC6423661
NM_174976ZDHHC221
NM_020815PCDH101
NM_032108SEMA6B1
NM_198427BCAN1
NM_178013PRIMA11
NM_003155STC11
XM_001130790KIAA18811
NM_001080400KIAA18811
NM_175895C12orf611
NM_022128RBKS1
NM_133490KCNG41
NM_014850SRGAP31
NM_001033117SRGAP31
NM_006291TNFAIP21
NM_000733CD3E1

Immunostimulatory motif search results


UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).

GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).



Settings used for search

Scores were calculated using scoring scheme SS1
Using AVG scoring mode.

Hit scores

ParameterSpec. Score cut-offColourComment
Hit to same geneColourHits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit.
High scoring hit≥80ColourHits are very likely to cause an off-target effect
Medium scoring hit<80¸≥40ColourHits with a small chance of causing an off target effect.
Low scoring hitColourHits very unlikely to cause an off-target effect

The mismatches are colour coded based on the weighting of that position.

Cut-offColour
MatchColour
Wobble base pairs (G-U)Colour
Critical bases (none)Colour
0.6 bitsColour
0.4 bitsColour
0.2 bitsColour

About SpecificityServer

SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).

Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer


For more details about the algorithm "siRNA specificity searching incorporating mismatch tolerance data" Bioinformatics (2008) 24 (10): 1316-1317