Predicted Off-targets for siRNA "gaacuaccauucaaggucu" Using siRNA SpecificityServer

Specificity Server [Result page v1.0]


SpecificityServer - An siRNA design program for determining siRNA specificity

Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands

10 hits were found for GAACUACCAUUCAAGGUCU (8 guide strand hits and 2 sense strand hits.)

Hits to Guide strand

AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
NM_152539 C3orf30 1770 1788 uAACUACCAUUCAAGuUuU69 3 14 c false   <90%   1814
NM_022037 TIA1 964 982 GuACUACCAUUgAAGGUCa49 3 9   false   <90%   2357
NM_022173 TIA1 997 1015 GuACUACCAUUgAAGGUCa49 3 9   false   <90%   2390
NM_020193 C11orf30 1906 1924 GAACgACCAUUCAAGGcCU25 2 11 c false   <90%   5518
NM_000035 ALDOB 490 508 aAACcACCAUUCAAGGgCU23 3 11 c false   <90%   1669
NM_181508 HPS5 209 227 uAgCUACCAgUCAAGGUCU-4 3 9 bc false   <90%   4536
NM_007216 HPS5 396 414 uAgCUACCAgUCAAGGUCU-4 3 9 bc false   <90%   4723
NM_181507 HPS5 553 571 uAgCUACCAgUCAAGGUCU-4 3 9 bc false   <90%   4880


Hits to Sense strand



AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
NM_022106 C20orf177 3419 3437 AGACCUUGAAUGGUuGagg44 4 14 c false   <90%   3825
NM_016621 PHF21A 3405 3423 AGACCUUGcAUGGUAGagu8 4 8 c false   <90%   3692


3 prime UTR Seed matches

Hits to same gene : 0
Hits to other genes : 917
View matched accessions

3 prime seed match details

AccessionGene nameHits to this gene
NM_030579CYB5B3
NM_024492LPAL23
NM_052832SLC26A72
NM_014880CD3022
NM_018019MED92
NM_017736THUMPD12
NM_144570HN1L2
NM_152750FLJ238342
NM_024577SH3TC22
NM_198887NUP432
NM_007331WHSC12
NM_014787DNAJC62
NM_000671ADH52
NM_001012981ZKSCAN22
NM_182749N6AMT12
NM_013240N6AMT12
XM_940069PPP1R3E2
XM_927029PPP1R3E2
NM_000550TYRP12
NM_003948CDKL22
NM_003048SLC9A22
NM_004845PCYT1B2
NM_004992MECP22
NM_015199ANKRD282
NM_000602SERPINE12
NM_0011016762
NM_145727LPAL22
NM_016946F11R2
NM_006203PDE4D2
NM_0011046312
NM_015430DKFZP586H21231
NM_001001991DKFZP586H21231
NM_024692CLIP41
NM_020914RNF2131
NM_058191C21orf661
NM_001039693FLJ379531
NM_1449891
NM_025048GPR1101
NM_004343CALR1
NM_024095ASB81
NM_001024660KALRN1
NM_007064KALRN1
NM_001001557GDF61
NM_006267RANBP21
NM_016356DCDC21
NM_012174FBXW81
NM_153348FBXW81
NM_022463NXN1
NM_001025300RAB121
NM_006516SLC2A11
NM_015544TMEM981
NM_001033504TMEM981
NM_022831C1orf801
NM_004272HOMER11
NM_020473PIGA1
NM_002641PIGA1
NM_015200SCC-1121
NM_0011003991
NM_178498SLC5A121
XM_944969LOC6491331
NM_002968SALL11
NM_001039716DIO11
NM_177539TAS1R11
NM_004277SLC25A271
NM_178127ANGPTL51
NM_025203C2orf441
NM_000362TIMP31
NM_080872UNC5D1
NM_020648TWSG11
NM_007220CA5B1
NM_0010854231
XM_001124643LOC7304161
NM_032974CASP101
NM_000599IGFBP51
NM_001561TNFRSF91
NM_001042520MGC130571
NM_001042521MGC130571
NM_001042519MGC130571
NM_032321MGC130571
NM_018211RAVER21
NM_021205RHOU1
NM_025193HSD3B71
NM_002356MARCKS1
NM_006706TCERG11
NM_001040006TCERG11
NM_001039905FLJ385961
NM_012153EHF1
NM_004520KIF2A1
NM_0010985111
NM_006713SUB11
NM_173641EPHA101
NM_001007033CLEC6A1
NM_018409LRP2BP1
NM_016073HDGFRP31
NM_152695ZNF4491
NM_153188TNPO11
NM_002270TNPO11
NM_001024596ZNF7721
NM_017856GEMIN81
NM_001042479GEMIN81
NM_001042480GEMIN81
NM_025237SOST1
NM_032449CC2D1B1
NM_080626BRI3BP1
NM_018264TYW11
NM_198270NHS1
XM_001134060NHS1
NM_032932RAB11FIP41
NM_001039395C9orf681
NM_018374TMEM106B1
NM_024940DOCK51
NM_001001701LOC4011521
XM_945031LOC6434641
XM_931563LOC6434641
NM_015216HISPPD11
NM_015158ANKRD151
NM_153186ANKRD151
NM_019044CCDC931
NM_015330SPECC1L1
NM_020754CDGAP1
NM_014765TOMM201
NM_198829RAC11
NM_006908RAC11
NM_018890RAC11
NM_080628C20orf1181
NM_015458MTMR91
NM_020151STARD71
NM_012342BAMBI1
XM_001129796LOC6455131
NM_153230FBXO391
NM_004348RUNX21
NM_001015051RUNX21
NM_001024630RUNX21
NM_152330FRMD61
NM_001042481FRMD61
NM_005385NKTR1
NM_053064GNG21
XM_001133421LOC7317741
XM_001132470LOC7290701
NM_006624ZMYND111
NM_170601SIAE1
NM_001008494ISX1
NM_032499C15orf411
NM_015533DAK1
NM_030803ATG16L11
NM_017974ATG16L11
NM_014622LOH11CR2A1
NM_018370DRAM1
NM_002940ABCE11
NM_001040876ABCE11
NM_001013694LOC4020551
XM_940362LOC6459841
XM_928946LOC6459841
NM_024430PSTPIP21
NM_133326ATP6V1G31
NM_001033723ZNF7041
NM_001032392PLGLB11
NM_002665PLGLB21
NM_002881RALB1
NM_015039NMNAT21
NM_170706NMNAT21
XM_001128001LOC7284011
XM_001127991LOC7284011
XM_001127568LOC7284991
XM_001127665LOC7285261
XM_001130826LOC7284521
XM_001130837LOC6433671
XM_932646LOC3400891
XM_001127715LOC7285401
XM_001127825LOC7285751
NM_173079RUNDC11
NM_017644KLHL241
NM_020825CRAMP1L1
NM_003913PRPF4B1
NM_001010917C10orf1321
NM_019616F71
NM_000131F71
NM_001083614EARS21
NM_000696ALDH9A11
NM_001007024GOSR11
NM_001007025GOSR11
NM_004871GOSR11
NM_025058TRIM461
NM_006275SFRS61
NM_014616ATP11B1
NM_000254MTR1
NM_017635SUV420H11
NM_004936CDKN2B1
NM_078487CDKN2B1
NM_015097CLASP21
NM_007054KIF3A1
NM_032372DYDC21
NM_006811SERINC31
NM_004290RNF141
NM_183400RNF141
NM_183398RNF141
NM_183401RNF141
NM_183399RNF141
NM_001079539XBP11
NM_005080XBP11
NM_201253CRB11
NM_014472C10orf281
NM_005434MALL1
NM_152280SYT111
NM_0011112831
NM_020119ZC3HAV11
NM_001080484KIAA17511
XM_936307KIAA17511
NM_000046ARSB1
NM_175900C16orf541
XM_001128758LOC7280701
NM_006375ENOX21
NM_182314ENOX21
NM_018000MREG1
NM_006251PRKAA11
NM_206907PRKAA11
NM_024646ZYG11B1
NM_004797ADIPOQ1
NM_144712SLC23A31
NM_020850RANBP101
NM_138350THAP31
XM_039733KIAA09531
XM_940464KIAA09531
NM_032714C14orf1511
NM_173471SLC25A261
XM_942502LOC6427571
XM_926793LOC6427571
NM_144997FLCN1
NM_001010867C1orf691
NM_001609ACADSB1
NM_006413RPP301
NM_031419NFKBIZ1
NM_001005474NFKBIZ1
NM_014573TMEM971
NM_198581ZC3H61
NM_198066GNPNAT11
NM_138473SP11
NM_012334MYO101
NM_181507HPS51
NM_007216HPS51
NM_181508HPS51
NM_003907EIF2B51
NM_000788DCK1
NM_000809GABRA41
NM_0011023961
NM_025073SIKE1
NM_017780CHD71
NM_002610PDK11
NM_012419RGS171
NM_153702ELMOD21
NM_005725TSPAN21
NM_001008783SLC35D31
NM_003129SQLE1
NM_018279TMEM191
NM_014743KIAA02321
NM_0011005901
NM_030791SGPP11
NM_052943FAM46B1
NM_152905NEDD11
NM_016653ZAK1
NM_007146VEZF11
NM_130847AMOTL11
NM_152321ERP271
NM_017650PPP1R9A1
NM_001012961C1orf841
NM_201999ELF21
NM_006874ELF21
XM_928022LOC6449351
XM_001130944LOC6449351
NM_003051SLC16A11
NM_017420SIX41
NM_013236ATXN101
NM_001003760KLHL311
NM_007214SEC631
NM_173808NEGR11
NM_152671PIP5K31
NM_0152831
NM_007053CD1601
NM_014381MLH31
NM_001040108MLH31
NM_004438EPHA41
XM_001129103LOC7290291
XM_001128912LOC7312381
NM_030627CPEB41
NM_001233CAV21
NM_198212CAV21
NM_001296CCBP21
NM_004829NCR11
XM_001131384LOC6465221
XM_933676LOC6465221
NM_022780RMND5A1
NM_018233OGFOD11
XM_001133171LOC7314771
NM_031885BBS21
NM_183419RNF191
NM_015435RNF191
NM_001007075KLHL51
NM_199039KLHL51
NM_015990KLHL51
NM_001023561ZNF7491
NM_003624RANBP31
NM_133464ZNF4831
NM_001310CREBL21
NM_0147221
NM_014333CADM11
NM_0010985171
NM_032946NXF51
NM_001901CTGF1
NM_002033FUT41
XM_940582LOC6514301
XM_001127939LOC6514301
NM_145307PLEKHK11
NM_153367C10orf561
NM_016205PDGFC1
NM_006016CD1641
NM_013943CLIC41
NM_007375TARDBP1
NM_020400GPR921
NM_0011045861
NM_024744ALS2CR81
NM_152391PQLC31
NM_012294RAPGEF51
NM_138694PKHD11
NM_018689KIAA11991
NM_144778MBNL21
NM_207304MBNL21
XM_059954C9orf571
XM_937019C9orf571
NM_001076786QSER11
NM_006933SLC5A31
NM_020786PDP21
NM_004896VPS26A1
NM_001035260VPS26A1
NM_015138RTF11
NM_033135PDGFD1
NM_025208PDGFD1
NM_014062NOB11
NM_001204BMPR21
NM_013987PARK21
NM_004562PARK21
NM_013988PARK21
NM_000875IGF1R1
NM_033051SLC46A21
NM_014791MELK1
XM_001133708LOC7297381
XM_001133805LOC7320461
NM_198935SS18L11
NM_080927DCBLD21
NM_001002006NT5C1B1
NM_033253NT5C1B1
NM_020116FSTL51
NM_005839SRRM11
NM_002655PLAG11
XM_001130189LOC7315981
XM_001129850LOC7292911
NM_001077619LOC1378861
NM_006606RBBP91
NM_002372MAN2A11
NM_021940SMAP11
NM_001044305SMAP11
NM_152291MUC71
NM_007034DNAJB41
NM_052880PIK3IP11
NM_016441CRIM11
NM_021168RAB40C1
NM_182574FLJ360701
NM_007043KRR11
XM_942869BOAT1
XM_292512BOAT1
NM_018110DOK41
NM_016373WWOX1
NM_002451MTAP1
NM_005497GJA71
NM_001080383GJA71
XM_001125875LOC7278181
NM_013269CLEC2D1
NM_001004419CLEC2D1
NM_020348CNNM11
NM_031962KRTAP9-31
NM_002279KRT33B1
NM_004469FIGF1
NM_022828YTHDC21
NM_014946SPAST1
NM_199436SPAST1
NM_001430EPAS11
NM_133374ZNF6181
NM_153045C9orf911
NM_014157CCDC1131
NM_198194STOM1
NM_004099STOM1
XM_001133778LOC7317101
XM_001129849LOC7282491
XM_927086LOC6533651
NM_206933USH2A1
NM_001821CHML1
NM_025218ULBP11
NM_014289CAPN61
NM_012199EIF2C11
NM_001003407ABLIM11
NM_001003408ABLIM11
NM_002313ABLIM11
NM_006720ABLIM11
NM_198718PTGER31
NM_0333901
NM_001039360ZBTB7C1
NM_003482MLL21
NM_007110TEP11
NM_015691WWC31
NM_198196CD961
NM_005816CD961
NM_001703BAI21
NM_002928RGS161
NM_153648SLC24A41
NM_153647SLC24A41
NM_153646SLC24A41
NM_005971FXYD31
NM_021910FXYD31
NM_004440EPHA71
NM_172071RC3H11
NM_177401MIDN1
NM_0011003981
NM_015085GARNL41
NM_004655AXIN21
NM_002745MAPK11
NM_022075LASS21
NM_181746LASS21
NM_022465IKZF41
NM_002198IRF11
XM_935586LOC6418451
XM_938850LOC6470871
XM_930101LOC6470871
NM_005626SFRS41
NM_005736ACTR1A1
NM_012316KPNA61
NM_152519FLJ238611
NM_032434ZNF5121
XM_928291LOC6452491
XM_935704LOC6452491
XM_938677LOC6452491
XM_053966LOC1132301
XM_937316LOC1132301
NM_021821MRPS351
NM_014790JAKMIP21
NM_020309SLC17A71
NM_015076CDC2L61
NM_017728TMEM1041
NM_031314HNRPC1
NM_001077443HNRPC1
NM_001077442HNRPC1
NM_004500HNRPC1
NM_001017408GOPC1
NM_020399GOPC1
NM_145012CCNY1
NM_032797AIFM21
NM_004200SYT71
XM_943686SYT71
NM_138348FAM105B1
NM_0010992941
XM_936510KIAA16441
NM_016531KLF31
NM_080415SEPT41
NM_021631FKSG21
NM_016511CLEC1A1
NM_004744LRAT1
NM_019117KLHL41
NM_001879MASP11
NM_173658ZNF6601
NM_018126TMEM331
NM_006611KLRA11
NM_000441SLC26A41
NM_020198CCDC471
NM_025205MED281
NM_007001SLC35D21
NM_003644GAS71
NM_201432GAS71
NM_201433GAS71
NM_024674LIN281
NM_173499SPATA81
NM_013291CPSF11
NM_147162IL11RA1
NM_032856WDR731
NM_005649ZNF354A1
NM_018477ACTR101
NM_003144SSR11
NM_001012977LOC3405291
NM_024894NOL101
NM_016376ANKFY11
XM_933050LOC3480211
XM_944169LOC3480211
NM_183372NBPF111
NM_0011016631
NM_030767AKNA1
NM_005715UST1
NM_006965ZNF241
NM_031450C11orf681
NM_004729ZBED11
NM_148899FOXP21
NM_014491FOXP21
NM_148898FOXP21
NM_017915C12orf481
NM_133445GRIN3A1
NM_004185WNT2B1
NM_024494WNT2B1
NM_018200HMG20A1
NM_024068OBFC2B1
NM_001039362ATP6V1C21
NM_144583ATP6V1C21
NM_022438MAL1
NM_022439MAL1
NM_022440MAL1
NM_002371MAL1
NM_020340KIAA12441
NM_004824CDYL1
NM_170751CDYL1
NM_170752CDYL1
NM_033449FCHSD11
NM_001005612EDA1
NM_001005610EDA1
NM_001040450FAM63B1
NM_001040453FAM63B1
NM_017676ZH2C21
NM_018351FGD61
NM_005187CBFA2T31
NM_175931CBFA2T31
NM_020714ZNF4901
NM_000609CXCL121
NM_018945PDE7B1
NM_015210KIAA08021
NM_019084CCNJ1
NM_153267MAMDC21
NM_006108SPON11
NM_032351MRPL451
XM_001126219MRPL451
NM_024763WDR781
NM_017811UBE2R21
NM_024944CHODL1
NM_000268NF21
NM_016418NF21
NM_181832NF21
NM_181828NF21
NM_181829NF21
NM_181833NF21
NM_181830NF21
NM_022153C10orf541
NM_031448C19orf121
NM_001031726C19orf121
NM_0249361
NM_001029998SLC10A71
NM_133647SLC12A61
NM_001042495SLC12A61
NM_001042494SLC12A61
NM_001042497SLC12A61
NM_005135SLC12A61
NM_001042496SLC12A61
NM_015064ERC11
NM_178039ERC11
NM_178040ERC11
NM_178038ERC11
NM_178037ERC11
NM_015394ZNF101
XM_001134358LOC7322731
NM_004302ACVR1B1
NM_152703SAMD9L1
NM_144599NIPA11
NM_014667VGLL41
NM_006364SEC23A1
NM_032512PDZD41
NM_001018075NR3C11
NM_001018077NR3C11
NM_001018076NR3C11
NM_001018074NR3C11
NM_001024094NR3C11
NM_000176NR3C11
NM_020909EPB41L51
NM_001030060SAMD51
NM_022562GH11
NM_022557GH21
NM_022644CSH21
NM_022640CSH11
NM_182970RIMS41
NM_153262SYT141
NM_001014372SYT14L1
NM_000348SRD5A21
NM_001018000KIAA10261
NM_001017999KIAA10261
NM_001018001KIAA10261
NM_015209KIAA10261
NM_031275TEX121
NM_015471NSL11
NM_001042549NSL11
NM_012395PFTK11
NM_001742CALCR1
NM_152680TMEM1541
NM_138554TLR41
NM_005036PPARA1
NM_001001928PPARA1
XM_927936LOC6448411
XM_935685LOC6419331
XM_938176LOC6448411
NM_014161MRPL181
NM_032826SLC35B41
NM_016275SELT1
NM_033251RPL131
NM_000977RPL131
NM_006154NEDD41
NM_198400NEDD41
NM_005538INHBC1
NM_000603NOS31
NM_006496GNAI31
NM_006402HBXIP1
NM_057749CCNE21
NM_001031700C4orf181
NM_016613C4orf181
NM_020993BCL7A1
NM_001024808BCL7A1
NM_017952PTCD31
NM_002645PIK3C2A1
NM_016525UBAP11
XM_001134020LOC7318651
NM_018046AGGF11
NM_001002814RAB11FIP11
NM_147189FAM110B1
NM_006955ZNF33B1
NM_020375C12orf51
NM_012446SSBP21
NM_177925H2AFJ1
NM_017738C9orf391
NM_004820CYP7B11
NM_019022TXNDC101
NM_006577B3GNT21
NM_001025252TPD521
NM_001025253TPD521
NM_005079TPD521
NM_213609FAM19A11
NM_133458ZFP901
NM_013256ZNF1801
NM_020154C15orf241
NM_001012756ZNF2601
NM_001077204CSPP11
NM_024790CSPP11
NM_004728DDX211
NM_013362ZNF2251
NM_015251ASCIZ1
NM_004263SEMA4F1
NM_032576CYorf15B1
NM_001010853ACY1L21
NM_016563RASL121
NM_152437ZNF6641
NM_000885ITGA41
NM_198493ANKRD451
NM_018096NLE11
NM_001014445NLE11
NM_001776ENTPD11
NM_0010981751
NM_173602DIP2B1
NM_000579CCR51
NM_0011001681
NM_0011055361
NM_014876JOSD11
NM_201546CDCP21
NM_030820COL21A11
NM_020214PARP61
NM_001013627LOC3405271
NM_013348KCNJ141
NM_170720KCNJ141
NM_030883OR2H11
NM_001005502CPM1
NM_198320CPM1
NM_001874CPM1
NM_006947SRP721
NM_018698NXT21
NM_173468MOBKL1A1
NM_000462UBE3A1
NM_130839UBE3A1
NM_130838UBE3A1
NM_001039396MPEG11
NM_012333MYCBP1
NM_001039547GK51
NM_003549HYAL31
NM_024946NIP301
NM_1526341
NM_014864FAM20B1
NM_003184TAF21
NM_001013439FXR11
NM_001013438FXR11
NM_005087FXR11
NM_016351ADAM221
NM_021723ADAM221
NM_021722ADAM221
NM_004923MTL51
NM_001013655LOC3898341
XM_001125792LOC7278341
XM_926013LOC6423981
NM_183065TMEM1071
NM_032354TMEM1071
NM_021109TMSB4X1
NM_183049TMSL31
NM_014701KIAA02561
NM_2073061
NM_001570IRAK21
NM_001033930UBA521
NM_003333UBA521
NM_017775TTC191
NM_004584RAD9A1
NM_001037339PDE4B1
NM_002600PDE4B1
NM_001037340PDE4B1
NM_001037341PDE4B1
NM_052941GBP41
NM_022462HIF3A1
NM_152794HIF3A1
NM_152795HIF3A1
NM_003201TFAM1
NM_005819STX61
NM_000305PON21
NM_001018161PON21
XM_938689KIAA16711
XM_371461KIAA16711
NM_000168GLI31
NM_152444ZADH11
NM_152367C1orf1611
XM_931447SETD51
XM_944298SETD51
XM_944295SETD51
XM_931444SETD51
NM_014661FAM53B1
NM_013238DNAJC151
XM_001134421C1orf1521
NM_001616ACVR2A1
NM_022167XYLT21
NM_000380XPA1
NM_001295CCR11
NM_002396ME21
NM_001744CAMK41
NM_032980DTNA1
NM_001390DTNA1
NM_032975DTNA1
NM_004393DAG11
NM_001034841LOC1620731
NM_018360CXorf151
NM_014862ARNT21
NM_020336KIAA12191
NM_024713C15orf291
NM_0011045461
NM_152431PIWIL41
NM_002664PLEK1
NM_018085IPO91
NM_012300FBXW111
NM_033644FBXW111
NM_033645FBXW111
NM_000349STAR1
NM_001007243STAR1
NM_001454FOXJ11
NM_201550LRRC101
NM_017923MARCH11
NM_000318PXMP31
NM_001079867PXMP31
XM_001125952LOC7278521
XM_001132609LOC7313661
NM_205855UNQ19401
NM_001106ACVR2B1
NM_144717IL20RB1
NM_001029875RGS7BP1
NM_014917NTNG11
NM_0011132281
NM_0011132261
NM_016422RNF1411
NM_016374ARID4B1
NM_031371ARID4B1
NM_020921NIN1
NM_004958FRAP11
XM_001132417LOC7312591
XM_001132664LOC7289871
NM_030637DDHD11
XM_943975FLJ390801
XM_499117FLJ390801
NM_022149MAGEF11
NM_015045WAPAL1
XM_940456FLJ221841
NM_001688ATP5F11
NM_145259ACVR1C1
NM_0011110311
NM_0011110331
NM_0011110321
NM_181846ZSCAN221
NM_020940KIAA16001
NM_000028AGL1
NM_000646AGL1
NM_000645AGL1
NM_000644AGL1
NM_000642AGL1
NM_000643AGL1
NM_014431KIAA12741
NM_001009922RCHY11
NM_001008925RCHY11
NM_015436RCHY11
NM_005373MPL1
NM_021954GJA31
NM_018602DNAJA41
NM_002716PPP2R1B1
NM_002440MSH41
NM_138288C14orf1471
NM_001012515FECH1
NM_000140FECH1
NM_024639ZNF322A1
NM_199005ZNF322B1
NM_003588CUL4B1
NM_001079872CUL4B1
XM_379623FLJ412001
XM_943473FLJ412001
NM_145037FAM55C1
NM_182554C10orf531
NM_024838THNSL11
NM_182551LYCAT1
NM_001002257LYCAT1
NM_0011001641
NM_0011001661
NM_014721PHACTR21
NM_0011001651
NM_024889C10orf811
NM_014943ZHX21
NM_015092SMG11
NM_004884PUNC1
XM_946032LOC6448521
XM_934218LOC6448521
NM_014982PCNX1
NM_004337OSGIN21
NM_198271LMOD31
NM_005188CBL1
NM_178585WDFY31
NM_014991WDFY31
NM_024808C13orf341
NM_021133RNASEL1
NM_002581PAPPA1
NM_0011016691
NM_003866INPP4B1
NM_182573LYPD51
NM_001031749LYPD51
NM_004737LARGE1
NM_133642LARGE1
NM_003758EIF3S11
NM_003787NOL41
NM_152275TTC30A1
NM_016052RRP151
NM_052918SORCS11
NM_001013031SORCS11
NM_0011004271
NM_021159RAP1GDS11
NM_0011004281
NM_0011004291
NM_0011004261
NM_0011004301
XM_930431LOC6451951
XM_942574LOC6451951
NM_023016ANKRD571
NM_005400PRKCE1
NM_003212TDGF11
NM_001077706LFDH1
NM_006777ZBTB331
NM_016218POLK1
NM_014648DZIP31
NM_000202IDS1
NM_004598SPOCK11
NM_016510SCLY1
NM_017437CPSF21
XM_001129158LIMS11
NM_017655GIPC21
NM_016081PALLD1
NM_144571CNOT6L1
NM_178496C3orf591
NM_017582UBE2Q11
NM_0142471
NM_001039199C20orf1211
NM_024331C20orf1211
NM_175854PAN31
NM_024942C10orf881
NM_003276TMPO1
NM_001045556SLA1
NM_006748SLA1
NM_001045557SLA1
NM_0010996781
NM_021156TXNDC131
NM_000888ITGB61
NM_206853QKI1
NM_206854QKI1
NM_206855QKI1
NM_021181SLAMF71
NM_005599NHLH21
NM_0011110611
NM_014911AAK11
NM_182526C14orf831
NM_017423GALNT71
NM_002750MAPK81
NM_139047MAPK81
NM_139046MAPK81
NM_139049MAPK81
NM_138737HEPH1
NM_014799HEPH1
XM_939208LOC6481491
XM_001133680LOC6481491
NM_002355M6PR1
NM_199044NSUN41
NM_152744SDK11
NM_012382TTC331
NM_018640LMO31
NM_001001395LMO31
NM_001080412ZBTB381
XM_001133510ZBTB381
NM_030885MAP41
NM_145231C14orf1431
NM_003247THBS21
NM_024548LRRIQ21
NM_0011111131
NM_005339HIP21
NM_0011111121
NM_203395IYD1
NM_002639SERPINB51

Immunostimulatory motif search results


UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).

GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).



Settings used for search

Scores were calculated using scoring scheme SS1
Using AVG scoring mode.

Hit scores

ParameterSpec. Score cut-offColourComment
Hit to same geneColourHits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit.
High scoring hit≥80ColourHits are very likely to cause an off-target effect
Medium scoring hit<80¸≥40ColourHits with a small chance of causing an off target effect.
Low scoring hitColourHits very unlikely to cause an off-target effect

The mismatches are colour coded based on the weighting of that position.

Cut-offColour
MatchColour
Wobble base pairs (G-U)Colour
Critical bases (none)Colour
0.6 bitsColour
0.4 bitsColour
0.2 bitsColour

About SpecificityServer

SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).

Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer


For more details about the algorithm "siRNA specificity searching incorporating mismatch tolerance data" Bioinformatics (2008) 24 (10): 1316-1317