Automatic recommendation
Not OK - siRNA is not specific
8 hits were found for GUGGUUGGACUCUUUCUAA (4 guide strand hits and 4 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_001009899 | KIAA2018 | 1598 | 1616 | a | U | G | G | U | U | G | G | A | C | U | C | U | U | U | C | U | u | g | 90 | 3 | 16 | ac | false | <90% | 13708 | ||
| NM_017515 | SLC35F2 | 1071 | 1089 | u | U | u | G | U | U | G | G | A | C | U | C | U | U | U | C | U | g | u | 70 | 4 | 14 | c | false | <90% | 2954 | ||
| NM_001004488 | OR2A25 | 736 | 754 | G | U | G | G | U | U | G | G | A | C | U | C | U | U | U | u | a | u | g | 42 | 4 | 15 | c | false | <90% | 933 | ||
| NM_004063 | CDH17 | 1288 | 1306 | a | U | G | G | a | U | G | G | A | C | U | C | U | U | c | C | U | A | A | 21 | 3 | 9 | c | false | <90% | 3697 | ||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_005761 | PLXNC1 | 1330 | 1348 | c | c | u | G | A | A | A | G | A | G | U | C | C | A | A | C | C | A | a | 62 | 4 | 15 | bc | false | <90% | 5121 | ||
| NM_003587 | DHX16 | 1302 | 1320 | g | c | A | G | A | A | A | G | A | G | U | C | C | A | u | C | C | A | g | 56 | 4 | 12 | c | false | <90% | 3377 | ||
| NM_015313 | ARHGEF12 | 1414 | 1432 | c | a | A | G | A | A | A | G | A | G | U | C | C | A | u | C | C | A | g | 56 | 4 | 12 | c | false | <90% | 9501 | ||
| NM_152750 | FLJ23834 | 2510 | 2528 | a | U | g | G | A | A | A | G | A | G | U | C | C | A | g | C | C | A | C | 53 | 3 | 11 | c | false | <90% | 3810 | ||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 1824
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer