Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands
11 hits were found for UGGAGUUAAUCAUCAACAU (8 guide strand hits and 3 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| XM_001130206 | LOC730892 | 291 | 309 | g | u | G | A | G | U | U | A | A | U | C | A | U | u | A | A | C | A | U | 67 | 3 | 11 | c | false | <90% | 647 | ||
| XM_001130313 | LOC728558 | 291 | 309 | g | u | G | A | G | U | U | A | A | U | C | A | U | u | A | A | C | A | U | 67 | 3 | 11 | c | false | <90% | 647 | ||
| NM_001686 | ATP5B | 755 | 773 | U | G | G | A | G | U | U | A | A | U | C | A | a | C | A | A | u | g | U | 63 | 3 | 12 | false | <90% | 1857 | |||
| NM_182537 | HTR3D | 114 | 132 | U | G | G | A | G | U | U | A | A | U | C | A | U | C | u | A | g | A | U | 51 | 2 | 14 | c | false | <90% | 1486 | ||
| NM_004494 | HDGF | 1679 | 1697 | U | G | u | A | G | U | U | A | A | U | C | A | U | C | A | A | g | A | g | 51 | 3 | 13 | c | false | <90% | 2376 | ||
| NM_172311 | SALF | 573 | 591 | U | G | a | A | G | U | U | A | A | U | C | c | U | C | A | A | C | A | g | 41 | 3 | 8 | false | <90% | 3824 | |||
| NM_006873 | STON1 | 469 | 487 | U | G | a | A | G | U | U | A | A | U | C | c | U | C | A | A | C | A | g | 41 | 3 | 8 | false | <90% | 5822 | |||
| NM_014706 | SART3 | 1411 | 1429 | U | G | G | A | G | U | U | g | A | U | C | A | U | C | A | A | g | u | a | 6 | 4 | 8 | c | false | <90% | 4377 | ||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| XR_015527 | LOC731571 | 198 | 216 | A | U | G | U | U | G | A | U | G | A | U | U | A | A | g | U | C | u | g | 67 | 3 | 14 | c | false | <90% | 1224 | ||
| XR_015307 | LOC728536 | 198 | 216 | A | U | G | U | U | G | A | U | G | A | U | U | A | A | g | U | C | u | g | 67 | 3 | 14 | c | false | <90% | 1224 | ||
| NM_014616 | ATP11B | 6212 | 6230 | A | U | G | U | U | G | A | U | a | A | U | U | A | A | C | U | u | a | c | 8 | 4 | 8 | c | false | <90% | 7368 | ||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 2529
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer