VIRsiRNAdb

SpecificityServer - An siRNA design program for determining siRNA specificity
Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands

25 hits were found for GUACCCAGAAAUUUGGGCG (18 guide strand hits and 7 sense strand hits.)

Hits to Guide strand

Accession

Gene

Start

End

Details of mismatches

Spec. Score

Mismatches

Contiguous

Jackson style specificity

Hits similar gene

HSP Score

HSP % Identity

HSP length

Gene Length

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

NM_015640

SERBP1

4751

4769

c

U

u

C

C

C

A

G

A

A

A

U

U

U

G

G

G

u

u

70

4

14

c

false

<90%

6701

NM_001018069

SERBP1

4769

4787

c

U

u

C

C

C

A

G

A

A

A

U

U

U

G

G

G

u

u

70

4

14

c

false

<90%

6719

NM_001018068

SERBP1

4796

4814

c

U

u

C

C

C

A

G

A

A

A

U

U

U

G

G

G

u

u

70

4

14

c

false

<90%

6746

NM_001018067

SERBP1

4814

4832

c

U

u

C

C

C

A

G

A

A

A

U

U

U

G

G

G

u

u

70

4

14

c

false

<90%

6764

XM_944502

FLJ31033

1486

1504

a

U

A

C

C

C

A

G

A

A

A

U

U

U

u

G

G

a

a

65

4

13

c

false

<90%

5925

XM_930969

FLJ31033

1486

1504

a

U

A

C

C

C

A

G

A

A

A

U

U

U

u

G

G

a

a

65

4

13

c

false

<90%

5925

XM_944501

FLJ31033

2195

2213

a

U

A

C

C

C

A

G

A

A

A

U

U

U

u

G

G

a

a

65

4

13

c

false

<90%

6733

XM_930988

FLJ31033

2384

2402

a

U

A

C

C

C

A

G

A

A

A

U

U

U

u

G

G

a

a

65

4

13

c

false

<90%

6919

NM_001080955

PLAGL1

778

796

G

U

g

C

C

C

A

G

A

g

A

U

U

U

G

G

G

C

G

-2

2

9

bc

false

<90%

2733

NM_001080956

PLAGL1

962

980

G

U

g

C

C

C

A

G

A

g

A

U

U

U

G

G

G

C

G

-2

2

9

bc

false

<90%

2917

NM_002656

PLAGL1

994

1012

G

U

g

C

C

C

A

G

A

g

A

U

U

U

G

G

G

C

G

-2

2

9

bc

false

<90%

2949

NM_001080951

PLAGL1

1329

1347

G

U

g

C

C

C

A

G

A

g

A

U

U

U

G

G

G

C

G

-2

2

9

bc

false

<90%

3284

NM_001080954

PLAGL1

1423

1441

G

U

g

C

C

C

A

G

A

g

A

U

U

U

G

G

G

C

G

-2

2

9

bc

false

<90%

3378

NM_001080953

PLAGL1

1424

1442

G

U

g

C

C

C

A

G

A

g

A

U

U

U

G

G

G

C

G

-2

2

9

bc

false

<90%

3379

NM_006718

PLAGL1

1456

1474

G

U

g

C

C

C

A

G

A

g

A

U

U

U

G

G

G

C

G

-2

2

9

bc

false

<90%

3411

NM_001080952

PLAGL1

1470

1488

G

U

g

C

C

C

A

G

A

g

A

U

U

U

G

G

G

C

G

-2

2

9

bc

false

<90%

3425

XM_934218

LOC644852

2348

2366

G

U

A

C

C

C

A

G

A

u

A

U

U

U

G

G

u

u

a

-13

4

9

c

false

<90%

3246

XM_946032

LOC644852

2348

2366

G

U

A

C

C

C

A

G

A

u

A

U

U

U

G

G

u

u

a

-13

4

9

c

false

<90%

3246

Hits to Sense strand

Accession

Gene

Start

End

Details of mismatches

Spec. Score

Mismatches

Contiguous

Jackson style specificity

Hits similar gene

HSP Score

HSP % Identity

HSP length

Gene Length

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

XR_019471

LOC389168

273

291

a

u

C

C

C

A

A

A

U

U

U

u

U

G

G

G

U

A

a

47

4

9

false

<90%

1763

XR_017613

LOC389168

273

291

a

u

C

C

C

A

A

A

U

U

U

u

U

G

G

G

U

A

a

47

4

9

false

<90%

1763

NM_003011

SET

235

253

a

u

C

C

C

A

A

A

U

U

U

u

U

G

G

G

U

A

a

47

4

9

false

<90%

2575

NM_001005182

OR6C1

229

247

u

a

C

C

C

A

A

g

U

U

U

C

U

G

G

G

U

A

a

19

4

10

bc

false

<90%

939

NM_024989

PGAP1

10346

10364

u

G

C

C

C

A

c

A

U

U

U

C

U

G

u

G

U

A

C

6

3

7

c

false

<90%

11113

NM_031915

SETDB2

2790

2808

u

G

C

C

C

A

A

A

U

c

U

C

U

u

G

G

U

A

C

-4

3

8

c

false

<90%

3307

NM_004573

PLCB2

2616

2634

u

G

C

C

C

u

A

A

a

U

U

C

U

G

G

G

U

A

C

-25

3

10

bc

false

<90%

4694

---

3 prime UTR Seed matches

Hits to same gene : 0
Hits to other genes : 1100
View matched accessions

3 prime seed match details

Accession	Gene name	Hits to this gene
NM_014895	KIAA1009	3
NM_144599	NIPA1	2
XM_942952	FLJ46284	2
XM_499123	FLJ46284	2
NM_017945	SLC35A5	2
NM_004273	CHST3	2
NM_052910	SLITRK1	2
NM_003421	ZNF37A	2
NM_001007094	ZNF37A	2
NM_001077523	AP3D1	2
NM_003938	AP3D1	2
NM_033331	CDC14B	2
NM_003671	CDC14B	2
NM_001077181	CDC14B	2
NM_024900	PHF17	2
NM_020844	KIAA1456	2
NM_001099677		2
NM_001013659	ZNF793	2
NM_001008537	KIAA2022	2
NM_001040152	PEG10	2
NM_153619	SEMA6D	2
NM_153618	SEMA6D	2
NM_153616	SEMA6D	2
NM_153617	SEMA6D	2
NM_020858	SEMA6D	2
NM_017415	KLHL3	2
NM_017594	DIRAS2	2
NM_181643	C1orf88	2
NM_012194		2
NM_133444	ZNF526	2
NM_183425	RBM38	2
NM_033133	CNP	2
NM_145255	MRPL10	2
NM_148887	MRPL10	2
NM_001042493	C6orf162	2
NM_020425	C6orf162	2
NM_006361	HOXB13	2
NM_003799	RNMT	2
NM_006059	LAMC3	2
NM_001037535	SCML1	2
NM_001037540	SCML1	2
NM_006746	SCML1	2
NM_001037536	SCML1	2
NM_152793	C7orf41	2
NM_033238	PML	2
NM_001099455		2
NM_018340	FLJ11151	2
NM_012396	PHLDA3	2
NM_152636	METT5D1	2
NM_017892		2
NM_015087	SPG20	2
NM_017813	IMPAD1	2
NM_139057	ADAMTS17	2
NM_001946	DUSP6	2
NM_022652	DUSP6	2
NM_014846	KIAA0196	1
NM_052896	CSMD2	1
NM_001007544	C1orf186	1
NM_022763	FNDC3B	1
NM_004112	FGF11	1
NM_000084	CLCN5	1
NM_024946	NIP30	1
NM_032499	C15orf41	1
NM_003983	SLC7A6	1
NM_001076785	SLC7A6	1
NM_014797	ZBTB24	1
NM_017847	C1orf27	1
NM_138773	SLC25A46	1
NM_024945	RMI1	1
NM_002444	MSN	1
NM_001039844	ACBD7	1
NM_032042	C5orf21	1
NM_018141	MRPS10	1
NM_017831	RNF125	1
NM_144970	CXorf38	1
NM_014945	ABLIM3	1
NM_012334	MYO10	1
NM_173510	CCDC117	1
NM_020775	KIAA1324	1
XM_001134195	LOC147646	1
XM_001134326	LOC147646	1
NM_152758	YTHDF3	1
NM_004050	BCL2L2	1
NM_000210	ITGA6	1
NM_001079818	ITGA6	1
NM_018945	PDE7B	1
NM_012309	SHANK2	1
NM_021626	SCPEP1	1
NM_182832	PLAC4	1
NM_173214	NFAT5	1
NM_006599	NFAT5	1
NM_138714	NFAT5	1
NM_001113178		1
NM_138713	NFAT5	1
NM_020703	AMIGO1	1
NM_014866	KIAA0310	1
XM_001134399	LOC730257	1
XM_001134398	LOC730256	1
NM_030780	SLC25A32	1
NM_001039762	FLJ45557	1
NM_198993	STAC2	1
NM_020810	TRMT5	1
NM_030761	WNT4	1
NM_014851	KLHL21	1
NM_014643		1
NM_005213	CSTA	1
NM_006378	SEMA4D	1
NM_003968	UBE1C	1
NM_198195	UBE1C	1
NM_178832	C10orf83	1
NM_001098831		1
NM_020799	STAMBPL1	1
NM_006572	GNA13	1
NM_207406	CCDC4	1
NM_152874	FAS	1
NM_152876	FAS	1
NM_152877	FAS	1
NM_152872	FAS	1
NM_152871	FAS	1
NM_152873	FAS	1
NM_000043	FAS	1
NM_152875	FAS	1
NM_016025	METTL9	1
NM_001077180	METTL9	1
NM_021912	GABRB3	1
NM_000814	GABRB3	1
XM_930678	LOC642441	1
NM_033439	IL33	1
NM_145727	LPAL2	1
NM_024492	LPAL2	1
NM_000619	IFNG	1
NM_001040146	CTF8	1
NM_001039690	CTF8	1
NM_001040144	CTF8	1
NM_014732	KIAA0513	1
NM_007007	CPSF6	1
NM_001099652		1
NM_005707	PDCD7	1
NM_000346	SOX9	1
NM_002119	HLA-DOA	1
NM_016623	FAM49B	1
NM_014752	SPCS2	1
NM_018227	UBE1L2	1
NM_001007097	NTRK2	1
NM_152384	BBS5	1
XM_001131828	BBS5	1
XM_001131846	BBS5	1
NM_005244	EYA2	1
NM_172113	EYA2	1
NM_172111	EYA2	1
NM_172110	EYA2	1
NM_172112	EYA2	1
NM_000910	NPY2R	1
NM_014521	SH3BP4	1
NM_057178	RFFL	1
NM_001017368	RFFL	1
XM_001127087	FLJ43950	1
NM_018302	C4orf19	1
NM_001104629		1
NM_000868	HTR2C	1
NM_003586	DOC2A	1
NM_005611	RBL2	1
NM_021135	RPS6KA2	1
NM_001006932	RPS6KA2	1
NM_014703	VPRBP	1
NM_000872	HTR7	1
NM_019859	HTR7	1
NM_019860	HTR7	1
NM_006424	SLC34A2	1
XM_001127362	KIAA0574	1
NM_018433	JMJD1A	1
NM_032446	MEGF10	1
NM_017703	FBXL12	1
NM_001001394	HCG3	1
NM_030937	CCNL2	1
NM_001039577	CCNL2	1
XM_001126014	LOC727877	1
NM_015245	ANKS1A	1
NM_020827		1
NM_019589		1
NM_001859	SLC31A1	1
NM_001081755	ELN	1
NM_001081754	ELN	1
NM_001081752	ELN	1
NM_000501	ELN	1
NM_001081753	ELN	1
XM_927936	LOC644841	1
XM_935685	LOC641933	1
XM_938176	LOC644841	1
NM_001682	ATP2B1	1
NM_001001323	ATP2B1	1
NM_014897	ZNF652	1
NM_001005502	CPM	1
NM_198320	CPM	1
NM_001874	CPM	1
NM_138769	RHOT2	1
XM_929714	LOC646762	1
XM_935722	LOC646762	1
XM_938418	LOC646762	1
NM_004078	CSRP1	1
NM_013336	SEC61A1	1
NM_007137	ZNF81	1
NM_003006	SELPLG	1
NM_003369	UVRAG	1
NM_022772	EPS8L2	1
XM_001129221	EPS8L2	1
NM_006224	PITPNA	1
NM_018367	PHCA	1
NM_020897	HCN3	1
NM_001098213		1
NM_001098212		1
NM_000861	HRH1	1
NM_001098211		1
NM_000342	SLC4A1	1
NM_003412	ZIC1	1
NM_033515	ARHGAP18	1
NM_022170	EIF4H	1
NM_031992	EIF4H	1
NM_033631	LUZP1	1
NM_003819	PABPC4	1
NM_014391	ANKRD1	1
NM_003889	NR1I2	1
NM_022002	NR1I2	1
NM_033013	NR1I2	1
NM_173625	C17orf78	1
NM_020342	SLC39A10	1
NM_014553	TFCP2L1	1
NM_003452	ZNF189	1
NM_197977	ZNF189	1
XM_001132797	LOC388630	1
XM_371250	LOC388630	1
NM_019600	KIAA1370	1
NM_014829	DDX46	1
NM_031889	ENAM	1
NM_173649	FLJ40172	1
NM_001483	GBAS	1
XM_378312	LOC283143	1
XM_943773	LOC283143	1
NM_138991	BACE2	1
NM_138992	BACE2	1
NM_012105	BACE2	1
NM_025185		1
NM_033091	TRIM4	1
NM_033017	TRIM4	1
NM_014522	PCDH11X	1
NM_003189	TAL1	1
NM_016079	VPS24	1
NM_001005753	VPS24	1
XM_001134431	ALS2CR16	1
NM_006729	DIAPH2	1
XM_001134454	ALS2CR16	1
NM_030817	APOLD1	1
NM_175900	C16orf54	1
XM_001128758	LOC728070	1
NM_015068	PEG10	1
NM_004772	C5orf13	1
NM_014296	CAPN7	1
NM_152470	RNF165	1
NM_000698	ALOX5	1
XM_001127464	ALOX5	1
NM_014950	ZBTB1	1
NM_198525	KIF7	1
NM_004995	MMP14	1
NM_033390		1
NM_006227	PLTP	1
NM_182676	PLTP	1
NM_015345	DAAM2	1
NM_002302	LECT2	1
NM_015879	ST8SIA3	1
NM_001039904	FLJ44838	1
NM_016428	ABI3	1
NM_014844	KIAA0329	1
NM_016245	HSD17B11	1
XM_001132265	DHRS8	1
NM_001033925	TIAL1	1
NM_003252	TIAL1	1
NM_001080529	WIPF3	1
XM_939315	LOC644150	1
XM_935705	LOC644150	1
NM_006355	TRIM38	1
NM_198066	GNPNAT1	1
NM_001039884	FLJ44894	1
NM_006670	TPBG	1
XM_379228	LOC401093	1
XM_944985	LOC401093	1
NM_006933	SLC5A3	1
NM_014014	ASCC3L1	1
NM_152519	FLJ23861	1
NM_017454	STAU1	1
NM_004602	STAU1	1
NM_017452	STAU1	1
NM_001037328	STAU1	1
NM_017453	STAU1	1
NM_199459	C10orf71	1
NM_019089	HES2	1
NM_014293	NPTXR	1
NM_020336	KIAA1219	1
NM_016352	CPA4	1
NM_007150		1
NM_018090	NECAP2	1
NM_000399	EGR2	1
NM_015326	SRGAP2	1
NM_007361	NID2	1
NM_032486	DCTN5	1
NM_000774	CYP2F1	1
XM_001131525	LOC731992	1
NM_001017370	LOC387921	1
NM_001012754	LOC387921	1
NM_031912	SYT15	1
NM_014856		1
NM_018194	HHAT	1
NM_152367	C1orf161	1
NM_207429	FLJ45803	1
NM_181714	C6orf152	1
NM_014468	VENTX	1
NM_001246	ENTPD2	1
NM_203468	ENTPD2	1
NM_003458	BSN	1
NM_031310	PLVAP	1
NM_016135	ETV7	1
NM_001029875	RGS7BP	1
NM_020879	KIAA1505	1
NM_006148	LASP1	1
NM_173079	RUNDC1	1
NM_022574	PERQ1	1
NM_000217	KCNA1	1
NM_006241	PPP1R2	1
NM_153687	IKIP	1
NM_014548	TMOD2	1
NM_007048	BTN3A1	1
NM_004884	PUNC	1
NM_153022	C12orf59	1
NM_080415	SEPT4	1
NM_001014797	KCNMA1	1
NM_017763	RNF43	1
NM_212503	PCTK3	1
NM_002596	PCTK3	1
NM_212502	PCTK3	1
NM_003612	SEMA7A	1
XM_928933	FLJ32154	1
XM_940524	FLJ32154	1
NM_022818	MAP1LC3B	1
NM_007033	RER1	1
NM_001077243	GRIA4	1
NM_000829	GRIA4	1
NM_001080115	LDB3	1
NM_001080116	LDB3	1
NM_013952	PAX8	1
NM_013953	PAX8	1
NM_013951	PAX8	1
NM_003466	PAX8	1
NM_013992	PAX8	1
NM_006242	PPP1R3D	1
NM_032189	ATP11A	1
NM_015205	ATP11A	1
NM_000425	L1CAM	1
NM_024003	L1CAM	1
NM_032506	KIAA1841	1
NM_173463	DKFZp761B107	1
NM_133482	RAD50	1
NM_005732	RAD50	1
NM_198141	GANC	1
NM_020796	SEMA6A	1
NM_005678	SNURF	1
NM_003478	CUL5	1
NM_012345	NUFIP1	1
NM_206909	PSD3	1
NM_015310	PSD3	1
NM_030660	ATXN3	1
NM_004993	ATXN3	1
NM_145012	CCNY	1
NM_181698	CCNY	1
NM_014385	SIGLEC7	1
NM_016543	SIGLEC7	1
NM_053003	SIGLEC12	1
NM_033329	SIGLEC12	1
NM_002245	KCNK1	1
XM_001124203	LOC730273	1
NM_001102454		1
NM_001101672		1
NM_016423	ZNF219	1
NM_006371	CRTAP	1
NM_001008388	ZCD2	1
NM_000680	ADRA1A	1
NM_020310	MNT	1
NM_002657	PLAGL2	1
NM_198477	UNQ473	1
NM_016169	SUFU	1
NM_012429	SEC14L2	1
NM_152581	MOSPD2	1
NM_152419		1
NM_004434	EML1	1
NM_001008707	EML1	1
NM_031474	NRIP2	1
NM_000545	TCF1	1
NM_152341	PAQR4	1
NM_002508	NID1	1
NM_001098721		1
NM_001098722		1
NM_004485	GNG4	1
NM_000349	STAR	1
NM_001007243	STAR	1
NM_015533	DAK	1
NM_017754	C6orf107	1
XM_930431	LOC645195	1
XM_942574	LOC645195	1
XM_001126476	LOC728073	1
XM_001131744	LOC732060	1
NM_002225	IVD	1
NM_000629	IFNAR1	1
NM_174953	ATP2A3	1
NM_174956	ATP2A3	1
NM_174954	ATP2A3	1
NM_174957	ATP2A3	1
NM_174958	ATP2A3	1
NM_005173	ATP2A3	1
NM_174955	ATP2A3	1
NM_005737	ARL4C	1
NM_207426	FLJ46831	1
XM_001126170	LOC730519	1
XM_001132260	LOC730060	1
NM_021229	NTN4	1
XM_001133364	LOC729533	1
XM_001133363	LOC729533	1
XM_001133365	LOC729533	1
NM_207418	FAM72A	1
NM_001100910		1
NM_022758	C6orf106	1
NM_024294	C6orf106	1
NM_003453	ZMYM2	1
NM_197968	ZMYM2	1
NM_005156	ROD1	1
NM_024585	ARMC7	1
NM_024933	ANKRD53	1
NM_198461	LONRF2	1
NM_138348	FAM105B	1
NM_007131	ZNF75	1
NM_006253	PRKAB1	1
XM_936831	IQSEC3	1
NM_170724	PKHD1	1
NM_014763	MRPL19	1
NM_177963	SYT12	1
NM_001001938	C9orf47	1
NM_001532	SLC29A2	1
NM_002846	PTPRN	1
NM_182848	CLDN10	1
NM_006984	CLDN10	1
NM_020918	GPAM	1
NM_032501	ACSS1	1
NM_015316	PPP1R13B	1
NM_013412	RABL2A	1
NM_006212	PFKFB2	1
NM_005337	NCKAP1L	1
NM_014614	PSME4	1
NM_033637	BTRC	1
NM_003939	BTRC	1
NM_138736	GNAO1	1
NM_000633	BCL2	1
NM_194298	SLC16A9	1
NM_016422	RNF141	1
NM_024659	GTDC1	1
NM_001006636	GTDC1	1
NM_014808	FARP2	1
NM_020410	ATP13A1	1
NM_001003665	C1orf95	1
NM_173595		1
NM_000246	CIITA	1
NM_173653	SLC9A9	1
NM_173558	FGD2	1
XM_001130825	LOC728357	1
XM_001132879	LOC731155	1
NM_005262	GFER	1
NM_015488	PNKD	1
NM_022572	PNKD	1
NM_001008408	RBM33	1
NM_053043	PRR8	1
NM_001099439		1
NM_019008	SMCR7L	1
NM_015192	PLCB1	1
NM_182734	PLCB1	1
NM_138621	BCL2L11	1
NM_006538	BCL2L11	1
NM_001031716	OBFC2A	1
NM_005096	ZMYM3	1
NM_201599	ZMYM3	1
NM_006089	SCML2	1
NM_001003760	KLHL31	1
NM_001048209	HNT	1
NM_016522	HNT	1
NM_014251	SLC25A13	1
NM_021137	TNFAIP1	1
NM_203329	CD59	1
NM_203331	CD59	1
NM_203330	CD59	1
NM_000611	CD59	1
NM_001011547	SLC5A9	1
NM_001010867	C1orf69	1
XM_944601	LOC651327	1
XM_373925	LOC388813	1
NM_020184	CNNM4	1
NM_006534	NCOA3	1
NM_181659	NCOA3	1
NM_020162	DHX33	1
NM_014953	DIS3	1
NM_018725	IL17RB	1
XM_001128367	LOC439949	1
XM_001129241	LOC439949	1
NM_052854	CREB3L1	1
NM_213604	ADAMTSL5	1
NM_152673	MUC20	1
NM_001098516		1
NM_004535	MYT1	1
NM_024546	C13orf7	1
NM_020733		1
NM_017770	ELOVL2	1
NM_022912	REEP1	1
NM_175876	EXOC8	1
NM_006708	GLO1	1
NM_006955	ZNF33B	1
NM_022831	C1orf80	1
XM_001134276	C1orf80	1
XM_930476	LOC653631	1
NM_024596	MCPH1	1
NM_207122	EXT2	1
NM_000401	EXT2	1
NM_005544	IRS1	1
NM_207346	TSEN54	1
XM_940667	SDC3	1
NM_001081461	JMJD6	1
NM_015641	TES	1
NM_152829	TES	1
NM_000576	IL1B	1
NM_015253	WSCD1	1
NM_001100423		1
NM_001100422		1
NM_001100424		1
NM_015535	LOC26010	1
NM_006843	SDS	1
NM_052861	MGC21675	1
XM_097977	FAM59B	1
XM_940578	FAM59B	1
NM_032801	JAM3	1
NM_024408	NOTCH2	1
XM_001133349	NOTCH2	1
NM_004584	RAD9A	1
NM_001007536	AKR1CL1	1
NM_012320	LYPLA3	1
NM_024687	FLJ23049	1
NM_033050	SUCNR1	1
NM_020783	SYT4	1
NM_004596	SNRPA	1
NM_182697	UBE2H	1
NM_003344	UBE2H	1
NM_003774	GALNT4	1
NM_023007	JMJD4	1
NM_032424	KIAA1826	1
NM_015278	SASH1	1
NM_173798	ZCCHC12	1
NM_213720	C22orf16	1
XM_001126125	PGAM5	1
XM_946032	LOC644852	1
XM_934218	LOC644852	1
NM_015187	KIAA0746	1
NM_024103	SLC25A23	1
NM_016217	HECA	1
NM_003023	SH3BP2	1
NM_139029	CD151	1
NM_004357	CD151	1
NM_001039490	CD151	1
NM_139030	CD151	1
NM_182484	BAGE5	1
NM_181704	BAGE4	1
NM_001187	BAGE	1
NM_005759	ABI2	1
XM_001126750	ABI2	1
NM_018313	PBRM1	1
NM_181042	PBRM1	1
NM_018165	PBRM1	1
NM_003580	NSMAF	1
NM_153456	HS6ST3	1
XM_944898	LOC651738	1
NM_004383	CSK	1
NM_001109903		1
NM_001001891	TMEM16G	1
NM_006613	GRAP	1
NM_207119	LRRC20	1
NM_018239	LRRC20	1
NM_018205	LRRC20	1
NM_001017995	SH3PXD2B	1
NM_015036		1
NM_001109760		1
NM_025161	C17orf70	1
NM_014249	NR2E3	1
NM_001076787	TP53I11	1
NM_006034	TP53I11	1
NM_207373	C10orf99	1
NM_018198	DNAJC11	1
NM_019044	CCDC93	1
NM_152374	FLJ38984	1
NM_018351	FGD6	1
NM_014571	HEYL	1
NM_139317	BIRC7	1
NM_022161	BIRC7	1
NM_145212	MRPL30	1
NM_015331	NCSTN	1
NM_001026213	CYP11B1	1
NM_000497	CYP11B1	1
NM_018440	PAG1	1
NM_017773	LAX1	1
NM_002569	FURIN	1
NM_001105	ACVR1	1
NM_019069	WDR5B	1
NM_012174	FBXW8	1
NM_153348	FBXW8	1
NM_012144	DNAI1	1
NM_024843	CYBRD1	1
NM_138356	SHF	1
NM_004852	ONECUT2	1
NM_016575	NT5DC3	1
NM_000798	DRD5	1
NM_014551	NCAPH2	1
NM_006293	TYRO3	1
NM_145865	ANKS4B	1
NM_017856	GEMIN8	1
NM_001042479	GEMIN8	1
NM_001042480	GEMIN8	1
NM_004937	CTNS	1
NM_173623	TTLL6	1
NM_001010931	HGF	1
NM_001010933	HGF	1
NM_002673	PLXNB1	1
NM_013332	HIG2	1
NM_001098786		1
NM_153701	IL12RB1	1
NM_003082	SNAPC1	1
NM_213589	RAPH1	1
NM_015061	JMJD2C	1
NM_005057	RBBP5	1
NM_007185	TNRC4	1
NM_005026	PIK3CD	1
NM_005957	MTHFR	1
XM_001131606	LOC731003	1
XM_001132798	LOC729040	1
NM_170774	RASSF2	1
NM_014737	RASSF2	1
NM_014437	SLC39A1	1
NM_001093	ACACB	1
NM_006650	CPLX2	1
NM_001008220	CPLX2	1
NM_002419	MAP3K11	1
NM_005451	PDLIM7	1
NM_203352	PDLIM7	1
NM_133496	SLC30A7	1
NM_203376	TMEM81	1
NM_005124	NUP153	1
NM_020367	PARP11	1
NM_178445	CCRL1	1
NM_016557	CCRL1	1
XM_941135	LOC651872	1
NM_007213	PRAF2	1
NM_001098833		1
NM_020218		1
NM_182764	ELMO2	1
NM_133171	ELMO2	1
XM_939663	KIAA1553	1
NM_001080450	KIAA1553	1
NM_174977	SEC14L4	1
NM_003657	BCAS1	1
NM_000433	NCF2	1
NM_145321	OSBPL3	1
NM_145320	OSBPL3	1
NM_145322	OSBPL3	1
NM_015550	OSBPL3	1
XM_001127604	NFATC2IP	1
NM_032815	NFATC2IP	1
NM_001669	ARSD	1
NM_014286	FREQ	1
NM_001079877	RASA4	1
NM_006989	RASA4	1
NM_001101404		1
NM_012239	SIRT3	1
NM_001017524	SIRT3	1
NM_000068	CACNA1A	1
NM_002241	KCNJ10	1
NM_001040107	HVCN1	1
NM_032369	HVCN1	1
NM_001031717	CRELD1	1
NM_001077415	CRELD1	1
NM_015513	CRELD1	1
XM_928199	LOC400581	1
XM_001134423	C9orf144	1
NM_000268	NF2	1
NM_016418	NF2	1
NM_181832	NF2	1
NM_181828	NF2	1
NM_181829	NF2	1
NM_181833	NF2	1
NM_181830	NF2	1
NM_015254	KIF13B	1
NM_001024843	TNRC6B	1
NM_015088	TNRC6B	1
NM_001003674	C18orf1	1
NM_181483	C18orf1	1
NM_181481	C18orf1	1
NM_001003675	C18orf1	1
NM_004338	C18orf1	1
NM_181482	C18orf1	1
NM_017590	ZC3H7B	1
NM_001076786	QSER1	1
NM_031469	SH3BGRL2	1
NM_138972	BACE1	1
NM_012104	BACE1	1
NM_138973	BACE1	1
NM_138971	BACE1	1
NM_001419	ELAVL1	1
NM_002146	HOXB3	1
NM_001111283		1
NM_000618	IGF1	1
NM_001111284		1
NM_001030287	ATF3	1
NM_001674	ATF3	1
NM_004024	ATF3	1
NM_001040619	ATF3	1
NM_003636	KCNAB2	1
NM_172130	KCNAB2	1
NM_145238	ZSCAN20	1
NM_173578	C11orf72	1
XM_942240	LOC650557	1
NM_001007024	GOSR1	1
NM_001007025	GOSR1	1
NM_004871	GOSR1	1
XM_938924	LOC644092	1
XM_001130314	LOC644092	1
NM_032875	FBXL20	1
XM_065166	KIAA1957	1
XM_939576	KIAA1957	1
NM_024954	UBTD1	1
NM_003211	TDG	1
NM_021156	TXNDC13	1
NM_147190	LASS5	1
NM_018240	KIRREL	1
NM_024989	PGAP1	1
NM_001163	APBA1	1
NM_001099294		1
XM_936510	KIAA1644	1
NM_152222	TNFRSF19L	1
NM_032871	TNFRSF19L	1
NM_018941	CLN8	1
NM_030927	TSPAN14	1
NM_030579	CYB5B	1
NM_178526	SLC25A42	1
NM_014747	RIMS3	1
NM_002339	LSP1	1
NM_001013255	LSP1	1
NM_001013253	LSP1	1
NM_001013254	LSP1	1
NM_015683	ARRDC2	1
NM_001025604	ARRDC2	1
XM_001132703	FLJ41047	1
XM_001133280	FLJ41047	1
NM_014079	KLF15	1
NM_024817	THSD4	1
NM_178173	CCDC36	1
NM_005789	PSME3	1
NM_176863	PSME3	1
NM_207397	CD164L2	1
NM_018943	TUBA8	1
NM_020133	AGPAT4	1
NM_001009922	RCHY1	1
NM_001008925	RCHY1	1
NM_015436	RCHY1	1
NM_003876	TMEM11	1
NM_005530	IDH3A	1
NM_138814	PNPLA5	1
NM_012295	CABIN1	1
NM_004204	PIGQ	1
NM_001100408		1
NM_001033046	C17orf62	1
NM_001100407		1
NM_012146	DUX1	1
NM_181050	AXIN1	1
NM_003502	AXIN1	1
NM_020354	ENTPD7	1
NM_006633	IQGAP2	1
NM_001347	DGKQ	1
NM_002032	FTH1	1
NM_152354	ZNF285A	1
NM_024875	SYNPO2L	1
NM_153823	GSG1	1
NM_031289	GSG1	1
NM_001080555	GSG1	1
NM_001080554	GSG1	1
NM_152855	IGLL1	1
NM_020070	IGLL1	1
NM_001013618	LOC91353	1
NM_001005473	PLCXD3	1
NM_022778	CCDC21	1
NM_023937	MRPL34	1
NM_007159	SLMAP	1
NM_173562	KCTD20	1
NM_173536	GABRG1	1
NM_005475	SH2B3	1
NM_002535	OAS2	1
NM_006624	ZMYND11	1
NM_002460	IRF4	1
NM_177424	STX12	1
NM_014744	TBC1D5	1
NM_001037165	FOXK1	1
NM_002055	GFAP	1
NM_152556	FLJ31818	1
NM_021141	XRCC5	1
NM_014615	KIAA0182	1
NM_005121	THRAP1	1
NM_000733	CD3E	1
NM_173551	ANKS6	1
NM_002547	OPHN1	1
NM_021615	CHST6	1
NM_021804	ACE2	1
NM_006035	CDC42BPB	1
NM_024318	LILRA6	1
NM_020340	KIAA1244	1
NM_006741	PPP1R1A	1
NM_052885	SLC2A13	1
NM_138328	RHBDL3	1
NM_013433	TNPO2	1
NM_012329	MMD	1
NM_001863	COX6B1	1
NM_022171	TCTA	1
XM_001132013	ANKH	1
NM_054027	ANKH	1
NM_003181	T	1
NM_152395	NUDT16	1
NM_032547	SCOC	1
NM_033090	GREB1	1
NM_022063	C10orf84	1
NM_005140	CNGA2	1
NM_001003682	TTMB	1
NM_001098816		1
NM_133374	ZNF618	1
NM_198124	CSMD3	1
NM_198123	CSMD3	1
NM_052900	CSMD3	1
NM_004960	FUS	1
NM_016089	ZNF589	1
NM_000584	IL8	1
NM_001259	CDK6	1
NM_024779	PIP5K2C	1
NM_130782	RGS18	1
NM_018374	TMEM106B	1
NM_025083	EDC3	1
NM_001012511	GOSR2	1
NM_004339	PTTG1IP	1
NM_015527	TBC1D10B	1
NM_005123	NR1H4	1
NM_002401	MAP3K3	1
NM_203351	MAP3K3	1
NM_001102575		1
NM_006999	POLS	1
NM_020800	IFT80	1
NM_014877	HELZ	1
NM_007054	KIF3A	1
NM_212558	LOC401498	1
NM_001011713	NAT12	1
NM_002182	IL1RAP	1
NM_182481	BAGE3	1
NM_182482	BAGE2	1
NM_004586	RPS6KA3	1
NM_002006	FGF2	1
NM_017482	ADD2	1
NM_017484	ADD2	1
NM_144498	OSBPL2	1
NM_014835	OSBPL2	1
NM_001003698	RREB1	1
NM_001003699	RREB1	1
NM_002955	RREB1	1
NM_138456	BATF2	1
XM_001129698	LOC729218	1
NM_005471	GNPDA1	1
NM_006313	USP15	1
NM_020335	VANGL2	1
NM_005587	MEF2A	1
NM_005795	CALCRL	1
NM_017952	PTCD3	1
NM_013374	PDCD6IP	1
NM_207444	FLJ35695	1
NM_001098402		1
NM_001098403		1
NM_020727	ZNF295	1
XM_001130815	LOC647859	1
NM_152990	PXT1	1
NM_025219	DNAJC5	1
NM_031954	KCTD10	1
NM_001039803	CCRK	1
NM_178432	CCRK	1
NM_012119	CCRK	1
NM_015044	GGA2	1
NM_021815	SLC5A7	1
NM_178313	SPTBN1	1
NM_001080975	REPS2	1
NM_004726	REPS2	1
NM_007365	PADI2	1
NM_002251	KCNS1	1
NM_001048198	SNHG3-RCC1	1
NM_001048197	SNHG3-RCC1	1
NM_001048195	RCC1	1
NM_001269	RCC1	1
NM_001048194	RCC1	1
NM_001048199	SNHG3-RCC1	1
NM_198527	HDDC3	1
NM_001707	BCL7B	1
NM_017759	FLJ20309	1
NM_020141	C1orf119	1
XM_001125727	LOC727778	1
XM_001125757	LOC727778	1
NM_001029861	NANOS2	1
NM_006674	HCP5	1
NM_001001685	FLJ45079	1
NM_004137	KCNMB1	1
NM_002868	RAB5B	1
NM_022823	FNDC4	1
NM_015511	C20orf4	1
NM_033141	MAP3K9	1
NM_080669	SLC46A1	1
NM_000321	RB1	1
NM_021197	WFDC1	1
NM_145351	SCARF1	1
NM_145350	SCARF1	1
NM_145349	SCARF1	1
NM_052950	WDFY2	1
NM_152464	C17orf32	1
NM_152238	SNX7	1
NM_015976	SNX7	1
NM_001102470		1
NM_001018111	PODXL	1
NM_005397	PODXL	1
NM_001018053	PFKFB2	1
NM_001012339	DNAJA5	1
NM_194283	DNAJA5	1
NM_198219	ING1	1
NM_198218	ING1	1
NM_005537	ING1	1
NM_198217	ING1	1
NM_012250	RRAS2	1
NM_001102669		1
NM_015092	SMG1	1
NM_015675	GADD45B	1
NM_014648	DZIP3	1
NM_017855	ODAM	1
NM_014231	VAMP1	1
NM_199245	VAMP1	1
NM_198560	LHFPL4	1
NM_006306	SMC1A	1
NM_002424	MMP8	1
NM_147129	ALS2CL	1
NM_182775	ALS2CL	1
NM_017637	BNC2	1
NM_000926	PGR	1
NM_020452	ATP8B2	1
NM_021191	NEUROD4	1
NM_021200	PLEKHB1	1
NM_022061	MRPL17	1
NM_199421	SOCS4	1
NM_080867	SOCS4	1
NM_004614	TK2	1
XM_942991	LOC642934	1
NM_000461	THRB	1
NM_031444	C22orf13	1
NM_016221	DCTN4	1
NM_018067	MAP7D1	1
NM_032052	PATZ1	1
NM_152308	C16orf75	1
NM_002978	SCNN1D	1
NM_000860	HPGD	1
NM_024629	MLF1IP	1
NM_020850	RANBP10	1
NM_194071	CREB3L2	1
NM_017489	TERF1	1
NM_003218	TERF1	1
NM_004992	MECP2	1
NM_018921	PCDHGA9	1
NM_018923	PCDHGB2	1
NM_018929	PCDHGC5	1
NM_002588	PCDHGC3	1
NM_018917	PCDHGA4	1
NM_018918	PCDHGA5	1
NM_032088	PCDHGA8	1
NM_018915	PCDHGA2	1
NM_018922	PCDHGB1	1
NM_003736	PCDHGB4	1
NM_018927	PCDHGB7	1
NM_018924	PCDHGB3	1
NM_018914	PCDHGA11	1
NM_018920	PCDHGA7	1
NM_018913	PCDHGA10	1
NM_018916	PCDHGA3	1
NM_018926	PCDHGB6	1
NM_003735	PCDHGA12	1
NM_032403	PCDHGC3	1
NM_032092	PCDHGA11	1
NM_018912	PCDHGA1	1
NM_018919	PCDHGA6	1
NM_018925	PCDHGB5	1
NM_018928	PCDHGC4	1
NM_024811	FLJ12529	1
NM_152284	CHMP4C	1
NM_198562	C3orf62	1
NM_014898	ZFP30	1
NM_019844	SLCO1B3	1
NM_138423	CASC4	1
NM_177974	CASC4	1
NM_198902	TSPAN3	1
NM_005724	TSPAN3	1
NM_020443	NAV1	1
NM_203343	EPB41	1
NM_203342	EPB41	1
NM_004437	EPB41	1
NM_006368	CREB3	1
NM_013380	ZNF228	1
NM_001083335	ZNF228	1
NM_032453		1
NM_012208	HARS2	1
NM_015553	PIP3-E	1
NM_015947	ASCC1	1
NM_152909	ZNF548	1
NM_016195	MPHOSPH1	1
NM_001392	DTNA	1
NM_012099	CD3EAP	1
NM_001866	COX7B	1
XM_944290	KIAA0492	1
XM_378914	KIAA0492	1
NM_001012720	RGR	1
NM_002921	RGR	1
NM_001012722	RGR	1
NM_001048249	DKFZP686E2158	1
NM_001083603	PTCH1	1
NM_001083607	PTCH1	1
NM_001083602	PTCH1	1
NM_001083605	PTCH1	1
NM_001083606	PTCH1	1
NM_001083604	PTCH1	1
NM_000264	PTCH1	1
NM_152890	COL24A1	1
NM_003658	BARX2	1
NM_015110	SMC5	1
NM_033224	PURB	1
NM_024516	C16orf53	1
NM_012287	CENTB2	1
NM_030808	NDEL1	1
NM_001025579	NDEL1	1
NM_016055	MRPL48	1
NM_015111	N4BP3	1
NM_174911	FAM84B	1
NM_130391	PTPRD	1
NM_130393	PTPRD	1
NM_130392	PTPRD	1
NM_002839	PTPRD	1
NM_001040712	PTPRD	1
NM_173167	UNC45B	1
NM_001033576	UNC45B	1
NM_133633	SYN3	1
NM_003490	SYN3	1
NM_007081	RABL2B	1
NM_001003789	RABL2B	1
NM_021991	JUP	1
NM_002230	JUP	1
NM_199131	VAX1	1
NM_003941	WASL	1
NM_005472	KCNE3	1
NM_004956	ETV1	1
NM_015090	NFASC	1
NM_016239	MYO15A	1
NM_020167	NMUR2	1
NM_017709	FAM46C	1
NM_024569	MPZL1	1
NM_003953	MPZL1	1
NM_000544	TAP2	1
NM_017645	FAM29A	1
NM_017654	SAMD9	1
NM_016544	RBJ	1
NM_032431	SYVN1	1
NM_172230	SYVN1	1
NM_017946	FKBP14	1
NM_015035	ZHX3	1
NM_014112	TRPS1	1
NM_015227	POFUT2	1
NM_133634	POFUT2	1
NM_133635	POFUT2	1
NM_000448	RAG1	1
NM_001008494	ISX	1
NM_021935	PROK2	1
XM_944029	LOC647979	1
NM_016220	ZNF588	1
NM_001013746	ZNF588	1
NM_030934	C1orf25	1
NM_016156	MTMR2	1
NM_201278	MTMR2	1
NM_201281	MTMR2	1
NM_014961	RUFY3	1
NM_012322	LSM5	1
NM_016269	LEF1	1

---

Immunostimulatory motif search results

UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).

GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).

---

---

Settings used for search

Scores were calculated using scoring scheme SS1
Using AVG scoring mode.

Hit scores

Parameter	Spec. Score cut-off	Colour	Comment
Hit to same gene		Colour	Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit.
High scoring hit	≥80	Colour	Hits are very likely to cause an off-target effect
Medium scoring hit	<80¸≥40	Colour	Hits with a small chance of causing an off target effect.
Low scoring hit		Colour	Hits very unlikely to cause an off-target effect

The mismatches are colour coded based on the weighting of that position.

Cut-off	Colour
Match	Colour
Wobble base pairs (G-U)	Colour
Critical bases (none)	Colour
0.6 bits	Colour
0.4 bits	Colour
0.2 bits	Colour

About SpecificityServer

SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).

Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer

For more details about the algorithm "siRNA specificity searching incorporating mismatch tolerance data" Bioinformatics (2008) 24 (10): 1316-1317