Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands
13 hits were found for AGGACCAGUGACUGAUGUU (5 guide strand hits and 8 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_012406 | PRDM4 | 1610 | 1628 | u | G | G | A | C | C | A | G | U | G | A | C | U | u | u | U | G | U | U | 53 | 3 | 12 | c | false | <90% | 4225 | ||
| NM_016608 | ARMCX1 | 1870 | 1888 | c | a | u | A | a | C | A | G | U | G | A | C | U | G | A | U | G | U | U | 15 | 4 | 14 | bc | false | <90% | 2141 | ||
| NM_023085 | CAPN10 | 828 | 846 | A | G | G | A | C | C | A | G | U | G | u | C | U | G | A | U | c | a | g | 7 | 4 | 10 | c | false | <90% | 2161 | ||
| NM_023083 | CAPN10 | 828 | 846 | A | G | G | A | C | C | A | G | U | G | u | C | U | G | A | U | c | a | g | 7 | 4 | 10 | c | false | <90% | 2626 | ||
| NM_001852 | COL9A2 | 2546 | 2564 | A | G | G | A | C | C | A | a | U | G | A | C | U | G | A | g | G | U | c | 6 | 3 | 7 | c | false | <90% | 2831 | ||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_001005334 | OR3A4 | 907 | 925 | u | g | C | A | U | C | A | G | U | C | A | C | U | G | u | U | C | C | U | 61 | 3 | 12 | c | false | <90% | 1047 | ||
| XM_936897 | KIAA1109 | 12483 | 12501 | c | A | C | u | U | C | A | G | U | C | A | C | U | G | G | U | C | g | a | 53 | 4 | 13 | c | false | <90% | 15440 | ||
| XM_371706 | KIAA1109 | 12618 | 12636 | c | A | C | u | U | C | A | G | U | C | A | C | U | G | G | U | C | g | a | 53 | 4 | 13 | c | false | <90% | 15575 | ||
| XM_001131075 | LOC731868 | 501 | 519 | c | c | C | A | g | C | A | G | U | C | A | C | U | G | G | U | C | C | c | 30 | 4 | 13 | bc | false | <90% | 738 | ||
| NM_203318 | MYO18A | 2745 | 2763 | c | c | C | A | U | C | A | G | U | C | c | C | U | G | G | U | C | C | g | 20 | 4 | 8 | c | false | <90% | 7546 | ||
| NM_078471 | MYO18A | 2745 | 2763 | c | c | C | A | U | C | A | G | U | C | c | C | U | G | G | U | C | C | g | 20 | 4 | 8 | c | false | <90% | 7591 | ||
| NM_016839 | RBMS1 | 612 | 630 | A | A | a | A | U | C | A | G | U | C | u | C | U | G | G | U | C | C | c | 14 | 3 | 7 | c | false | <90% | 2596 | ||
| NM_152375 | KLHDC7A | 2824 | 2842 | A | A | C | A | U | C | A | G | c | C | A | C | U | G | G | g | C | C | c | 8 | 3 | 8 | c | false | <90% | 5070 | ||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 2015
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer