Predicted Off-targets for siRNA "aagggcuaucaaccuauagau" Using siRNA SpecificityServer

Specificity Server [Result page v1.0]


SpecificityServer - An siRNA design program for determining siRNA specificity

Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands

2 hits were found for GGGCUAUCAACCUAUAGAU (0 guide strand hits and 2 sense strand hits.)

Hits to Sense strand



AccessionGeneStartEndDetails of mismatchesSpec.
Score
MismatchesContiguousJackson style
specificity
Hits similar
gene
HSP
Score
HSP %
Identity
HSP lengthGene Length
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
NM_153358 ZNF791 2054 2072 ccaUAUAGGUUGgUAGCCC56 4 9   false   <90%   2476
NM_018246 CCDC25 2469 2487 AcuUAUAGGUUGAUuGCCC44 3 11 c false   <90%   3653


3 prime UTR Seed matches

Hits to same gene : 0
Hits to other genes : 922
View matched accessions

3 prime seed match details

AccessionGene nameHits to this gene
NM_015053PPFIA43
NM_001004313LOC3883352
NM_001029863C6orf1202
NM_015831ACHE2
NM_005121THRAP12
NM_001024596ZNF7722
NM_001037293PALM22
NM_053016PALM22
NM_001759CCND22
NM_019044CCDC932
NM_001025079CD472
NM_001777CD472
NM_198793CD472
NM_006061CRISP32
NM_024577SH3TC22
NM_176823S100A7A2
NM_018196TMLHE2
NM_001432EREG2
NM_199245VAMP12
NM_005957MTHFR2
NM_173598KSR22
NM_032208ANTXR12
NM_078483SLC36A12
NM_033387FAM78A2
NM_014976PDCD112
NM_001034853RPGR1
NM_019595ITSN21
NM_006277ITSN21
NM_198904GABRG21
NM_000816GABRG21
NM_198903GABRG21
XM_928199LOC4005811
NM_012258HEY11
NM_001040708HEY11
NM_001025249DUT1
NM_001025248DUT1
NM_001948DUT1
XM_934118LOC6534891
XM_934115LOC6534891
NM_0011055731
NM_006496GNAI31
NM_007027TOPBP11
NM_0149871
NM_153764KCNJ11
NM_153766KCNJ11
NM_000220KCNJ11
NM_153765KCNJ11
NM_153767KCNJ11
XM_932564LOC6449901
XM_944438LOC6509611
NM_020673RAB22A1
NM_004505USP61
XM_939750USP321
NM_032582USP321
NM_024345WDR321
NM_000665ACHE1
NM_017832C9orf61
NM_080628C20orf1181
NM_182898CREB51
NM_004904CREB51
NM_001011666CREB51
NM_182899CREB51
NM_207173NPSR11
NM_001002926TWISTNB1
NM_016356DCDC21
NM_013402FADS11
NM_182559TMPRSS121
NM_171998RAB39B1
NM_001001684FLJ458311
NM_005164ABCD21
NM_002223ITPR21
NM_0011004211
NM_0011004201
NM_017447C21orf911
NM_024613PLEKHF21
NM_152332MTAC2D11
NM_172164NASP1
NM_002482NASP1
NM_152298NASP1
NM_014238KSR11
NM_006113VAV31
NM_001079874VAV31
NM_152443RDH121
NM_0195891
NM_005897IPP1
NM_145207SPATA51
NM_130809PRRC11
NM_003183ADAM171
NM_032859ABHD131
NM_014373GPR1601
NM_181843NUDT81
NM_005629SLC6A81
NM_012174FBXW81
NM_153348FBXW81
NM_198087ZNF2001
NM_198088ZNF2001
NM_003454ZNF2001
NM_000412HRG1
NM_015215CAMTA11
NM_199328CLDN81
NM_025112ZXDC1
NM_020148SPIRE11
NM_003811TNFSF91
NM_001042599ERBB41
NM_005235ERBB41
NM_0209101
NM_020384CLDN21
NM_207467FLJ355301
NM_014647KIAA04301
NM_019106SEPT31
NM_013231FLRT21
NM_054111IHPK31
NM_001007097NTRK21
NM_201437TCEA11
NM_006756TCEA11
NM_080591PTGS11
NM_000962PTGS11
NM_174968ST3GAL31
NM_174971ST3GAL31
NM_174964ST3GAL31
NM_174963ST3GAL31
NM_174969ST3GAL31
NM_174965ST3GAL31
NM_174966ST3GAL31
NM_174970ST3GAL31
NM_006279ST3GAL31
NM_174967ST3GAL31
NM_199286DPPA31
NM_017680ASPN1
NM_001859SLC31A11
NM_016507CRKRS1
NM_0150831
NM_018052VAC141
NM_0010992201
NM_004737LARGE1
NM_133642LARGE1
NM_0010985091
NM_014853RUTBC11
NM_138578BCL2L11
NM_001191BCL2L11
NM_001773CD341
NM_014604TAX1BP31
NM_015037KIAA09131
NM_020362C1orf1281
NM_020643C11orf161
NM_014518ZNF2291
NM_173065IL28RA1
NM_173064IL28RA1
NM_170743IL28RA1
XM_942673FLJ443791
XM_946212FLJ443791
XM_059578FLJ443791
NM_001080537FLJ443791
NM_144582TEX2611
NM_152260RPUSD21
NM_001040285PAPD51
NM_001040284PAPD51
NM_001144AMFR1
NM_1829691
NM_001024401SBK11
NM_005578LPP1
NM_006097MYL91
NM_181526MYL91
NM_018373SYNJ2BP1
NM_173468MOBKL1A1
NM_032195SON1
NM_052876BTBD14B1
NM_018393TCP11L11
NM_014711CP1101
NM_021127PMAIP11
NM_015015JMJD2B1
NM_012229NT5C21
NM_144607CYB5D11
NM_004703RABEP11
NM_001083585RABEP11
NM_014458KLHL201
NM_001017926ZHX11
NM_007222ZHX11
NM_017952PTCD31
NM_182487OLFML2A1
NM_0011101991
NM_001821CHML1
NM_0011024451
NM_005613RGS41
XM_942780SYNPO21
NM_021961TEAD11
NM_139235NOL61
NM_022917NOL61
NM_006656NEU31
NM_006912RIT11
NM_174938FRMD31
NM_001014342FLG21
NM_198388CACNA1G1
NM_198387CACNA1G1
NM_198396CACNA1G1
NM_198378CACNA1G1
NM_198379CACNA1G1
NM_198380CACNA1G1
NM_198382CACNA1G1
NM_198385CACNA1G1
NM_018896CACNA1G1
NM_198377CACNA1G1
NM_198386CACNA1G1
NM_198376CACNA1G1
NM_198384CACNA1G1
NM_198383CACNA1G1
NM_000391TPP11
NM_000687AHCY1
XM_001132080LOC7310071
NM_203464AK3L11
NM_013410AK3L11
NM_001005353AK3L11
XM_928632LOC6456191
NM_198682GYPE1
NM_004985KRAS1
NM_033360KRAS1
NM_016441CRIM11
NM_005065SEL1L1
NM_001652AQP61
NM_000330RS11
NM_001077628APH1A1
NM_016022APH1A1
NM_020810TRMT51
NM_002023FMOD1
NM_002263KIFC11
NM_003144SSR11
XM_293918LOC3456431
XM_938591LOC3456431
NM_000251MSH21
NM_006915RP21
NM_007325GRIA31
NM_000828GRIA31
NM_014382ATP2C11
NM_012434SLC17A51
NM_138417KTI121
NM_031372HNRPDL1
NM_014299BRD41
NM_001033055AIPL11
NM_001033054AIPL11
NM_014336AIPL11
NM_006581FUT91
NM_178314FLJ393781
NM_005399PRKAB21
NM_033551LARP11
NM_015315LARP11
NM_032193RNASEH2C1
NM_025090USP361
NM_001037954DIXDC11
NM_033425DIXDC11
NM_013989DIO21
NM_001007023DIO21
NM_000793DIO21
NM_0010987871
NM_016526BET1L1
NM_000958PTGER41
NM_014891PDAP11
NM_018246CCDC251
NM_002092GRSF11
NM_0010984771
NM_016076C1orf1211
NM_198990NAPE-PLD1
NM_001013690LOC4017201
NM_001543NDST11
NM_175888ZNF6201
NM_152765C8orf461
XM_001125756LOC7277881
XM_001125734LOC7277811
XM_001127087FLJ439501
NM_005551KLK21
NM_001002231KLK21
NM_003482MLL21
NM_0010982131
NM_0010982121
NM_000861HRH11
NM_0010982111
NM_172020POM1211
XM_001128149LOC7303161
XM_001129924LOC7293161
NM_133458ZFP901
NM_022169ABCG41
NM_153487MDGA11
NM_006521TFE31
NM_017551GRID11
NM_0010984841
NM_003759SLC4A41
NM_015886PI151
XM_940609FLJ250761
XM_059689FLJ250761
NM_052965C1orf191
NM_080647TBX11
NM_016298FBXO401
XM_001126326AGRIN1
NM_198576AGRN1
NM_001003940BMF1
NM_001003943BMF1
NM_001003942BMF1
NM_033503BMF1
NM_001040458ARTS-11
NM_018367PHCA1
NM_001287CLCN71
NM_181491SURF51
XM_001133467PKD1L31
XM_001134223PKD1L31
NM_152372MYOM31
NM_153354TMEM161B1
NM_0011096891
NM_021061ZNF2501
NM_177550SLC13A51
NM_020336KIAA12191
NM_033484FBXO41
NM_001005473PLCXD31
NM_001008215MGC521101
NM_139132NUP981
NM_016320NUP981
NM_006987RPH3AL1
NM_000599IGFBP51
NM_003493HIST3H31
NM_133638ADAMTS191
NM_003706PLA2G4C1
NM_003216TEF1
NM_000748CHRNB21
NM_004914RAB361
NM_182935MOBP1
XM_942692MGC51391
NM_001080533MGC51391
NM_015460MYRIP1
NM_0010099841
NM_014437SLC39A11
NM_001083614EARS21
NM_145303LOC2024591
NM_020818KIAA14091
NM_002673PLXNB11
NM_016169SUFU1
NM_002203ITGA21
NM_0011055331
NM_000272NPHP11
NM_005841SPRY11
NM_199327SPRY11
NM_002579PALM1
NM_001040134PALM1
XM_001129841LOC4409001
XM_498909LOC4409001
NM_005704PTPRU1
NM_133177PTPRU1
NM_133178PTPRU1
NM_018110DOK41
NM_004836EIF2AK31
NM_001010887ASAH3L1
NM_004635MAPKAPK31
NM_032047B3GNT51
XM_945000LOC6454341
XM_932897LOC6454341
NM_198560LHFPL41
XM_937761LOC2840671
XM_208990LOC2840671
NM_001077484SLC38A11
NM_030674SLC38A11
NM_005159ACTC11
NM_020208SLC6A201
NM_022405SLC6A201
NM_017943FBXO341
NM_021939FKBP101
NM_003114SPAG11
NM_172218SPAG11
NM_014911AAK11
NM_183059RD31
NM_014635GCC21
NM_181453GCC21
NM_005054RGPD51
XM_001126221LOC7278511
XM_001126260LOC7278511
XM_001133591LOC7295401
XM_001133590LOC7295401
XM_001126233LOC7278511
XM_001126248LOC7278511
XM_001126272LOC7278511
XM_929931LOC6534891
XM_001130020KIAA11471
XM_001130081KIAA11471
NM_001080392KIAA11471
NM_020836KIAA14461
NM_0011051981
NM_030577TMEM1771
NM_0011051991
NM_0010996261
NM_019557FAM54B1
NM_0010996271
NM_0010996251
NM_017741C4orf301
NM_0011107811
NM_032983CASP21
NM_0010986721
NM_002243KCNJ151
NM_170736KCNJ151
NM_170737KCNJ151
NM_173195KCNIP21
NM_173194KCNIP21
NM_173191KCNIP21
NM_014591KCNIP21
NM_173192KCNIP21
NM_173193KCNIP21
NM_032433ZNF3331
NM_016032ZDHHC91
NM_001008222ZDHHC91
NM_001008393LOC2017251
NM_015633FGFR1OP21
NM_032575GLIS21
NM_0010987211
NM_0010987221
NM_004485GNG41
NM_033339CASP71
NM_001227CASP71
NM_033340CASP71
NM_033338CASP71
NM_015678NBEA1
NM_006869CENTA11
NM_001001349NKIRAS21
NM_017595NKIRAS21
NM_007038ADAMTS51
NM_006054RTN31
NM_201429RTN31
NM_201428RTN31
NM_201430RTN31
NM_007283MGLL1
NM_001003794MGLL1
NM_001080485ZNF2751
NM_145044ZNF5011
NM_001009909LUZP21
NM_001790CDC25C1
NM_022809CDC25C1
NM_002666PLIN1
NM_004067CHN21
NM_001039936CHN21
NM_012121CDC42EP41
NM_000104CYP1B11
NM_130848C5orf201
NM_133463AMZ11
NM_182894CHX101
NM_198486RPL7L11
NM_003034ST8SIA11
NM_001012393OPCML1
NM_002545OPCML1
NM_016575NT5DC31
XM_001133696LOC7316801
NM_144579SFXN51
NM_003898SYNJ21
NM_173811C11orf771
NM_015358MORC31
NM_0011013871
NM_030630C17orf281
NM_014893NLGN4Y1
NM_020742NLGN4X1
NM_181332NLGN4X1
NM_020901KIAA15421
XM_945436LOC4009581
XM_379100LOC4009581
NM_025195TRIB11
NM_000743CHRNA31
NM_021044DHH1
NM_000877IL1R11
NM_001010867C1orf691
NM_000624SERPINA51
NM_002155HSPA61
NM_030665RAI11
NM_018011FLJ101541
NM_017677MTMR81
NM_001106ACVR2B1
NM_019107C19orf101
NM_014310RASD21
NM_174914UGT3A21
NM_001033518WIPI21
NM_001033519WIPI21
NM_015610WIPI21
NM_001033520WIPI21
NM_016003WIPI21
NM_018440PAG11
NM_015084MRPS271
NM_022170EIF4H1
NM_031992EIF4H1
NM_0010976111
NM_001962EFNA51
NM_173174PTK2B1
NM_004103PTK2B1
NM_173175PTK2B1
NM_173176PTK2B1
NM_024766C2orf341
NM_198269HIPK11
NM_181358HIPK11
NM_198268HIPK11
NM_022480KLHL251
NM_001012418LOC3401561
NM_198681PLEKHG51
NM_020631PLEKHG51
NM_001042664PLEKHG51
NM_001042663PLEKHG51
NM_001042665PLEKHG51
NM_182757IBRDC21
NM_178519C17orf551
NM_004395DBN11
NM_080881DBN11
NM_003133SRP91
XM_927451LOC6532261
NM_004012DMD1
NM_004016DMD1
NM_004009DMD1
NM_004017DMD1
NM_000109DMD1
NM_004011DMD1
NM_004015DMD1
NM_004023DMD1
NM_004022DMD1
NM_004020DMD1
NM_004021DMD1
NM_004007DMD1
NM_004006DMD1
NM_004013DMD1
NM_004014DMD1
NM_004018DMD1
NM_004010DMD1
NM_013975LIG31
NM_002359MAFG1
NM_032711MAFG1
NM_000355TCN21
NM_025193HSD3B71
NM_014402UQCRQ1
NM_181489ZNF4451
NM_015456COBRA11
XM_001133009COBRA11
NM_003165STXBP11
NM_001032221STXBP11
NM_020672S100A141
NM_005831CALCOCO21
NM_014063DBNL1
NM_001014436DBNL1
NM_020972ZFYVE281
NM_024327ZNF5761
NM_001077199SFRS121
NM_139168SFRS121
NM_001337CX3CR11
NM_006861RAB351
NM_033012TNFSF111
NM_003701TNFSF111
NM_002030FPRL21
NM_005730CTDSP21
NM_015259ICOSLG1
NM_015005KIAA02841
NM_0011127261
NM_022745ATPAF11
NM_001042546ATPAF11
NM_001626AKT21
NM_002501NFIX1
NM_175736FMNL31
NM_198900FMNL31
XM_001128273LOC7287191
NM_032554GPR811
NM_021806FAM3A1
XM_001130382LOC7308551
XM_001134429MUC5AC1
NM_001012270BIRC51
NM_001012271BIRC51
NM_001168BIRC51
XM_939919LOC6508321
NM_002756MAP2K31
NM_145109MAP2K31
NM_014069PSORS1C21
NM_0147221
XM_941162LOC4009501
XM_376049LOC4009501
NM_148886SMCR71
NM_139162SMCR71
NM_003189TAL11
NM_021033RAP2A1
XM_001128260LOC7287151
XM_001128633LOC7311581
NM_031409CCR61
NM_004367CCR61
NM_006041HS3ST3B11
NM_005652TERF21
NM_024843CYBRD11
NM_001640APEH1
NM_005269GLI11
XM_378462FLJ224471
NM_002028FNTB1
NM_007214SEC631
NM_001081491NXF11
NM_007195POLI1
NM_001017416USP11
NM_001017415USP11
NM_003368USP11
NM_001155ANXA61
NM_004033ANXA61
NM_173629C18orf261
NM_015332NUDCD31
NM_018100EFHC11
XM_001134401LOC7302581
NM_017882CLN61
NM_012316KPNA61
NM_0251851
NM_003011SET1
NM_133334WHSC11
NM_001109ADAM81
NM_001080497MEGF91
XM_936860MEGF91
NM_001005361DNM21
NM_004945DNM21
NM_001005362DNM21
NM_001005360DNM21
NM_152734C6orf891
NM_152253CHKB1
NM_181425FXN1
NM_000144FXN1
NM_015341NCAPH1
NM_002595PCTK21
NM_017896C20orf111
NM_006696BRD81
NM_183359BRD81
XM_001131034LOC7315151
XM_001131024LOC7315151
NM_177963SYT121
NM_003149STAC1
NM_005487HMG2L11
NM_014250HMG2L11
NM_001003681HMG2L11
NM_005500SAE11
NM_001024843TNRC6B1
NM_015088TNRC6B1
NM_005471GNPDA11
NM_021783EDA2R1
NM_014830ZBTB391
NM_178342C3orf351
NM_003251THRSP1
NM_005902SMAD31
NM_015143METAP11
NM_007190SEC23IP1
NM_004862LITAF1
NM_003372VBP11
NM_002310LIFR1
NM_018400SCN3B1
NM_001040151SCN3B1
NM_198404KCTD41
NM_014906PPM1E1
NM_014231VAMP11
NM_003463PTP4A11
NM_015471NSL11
NM_001042549NSL11
XM_939663KIAA15531
NM_001080450KIAA15531
NM_022549FEZ11
NM_015576ERC21
NM_012262HS2ST11
NM_012395PFTK11
NM_052886MAL21
NM_032160DSEL1
NM_001002811PDE4DIP1
NM_001002812PDE4DIP1
XM_001126165LOC7279271
NM_014035SNX241
NM_001005337PKP11
NM_000299PKP11
NM_002677PMP21
NM_001722POLR3D1
NM_001037164ARL4A1
NM_005738ARL4A1
NM_212460ARL4A1
NM_000130F51
XM_001134421C1orf1521
NM_052811TRIM131
NM_213590TRIM131
NM_001007278TRIM131
NM_005798TRIM131
NM_032423ZNF5281
NM_139164STARD41
NM_0146951
NM_152608C1orf551
NM_014829DDX461
NM_139161CRB31
NM_174881CRB31
NM_002731PRKACB1
NM_182948PRKACB1
NM_173496MPP71
NM_015101GLT25D21
NM_181836TMED71
XM_934514LOC6447141
XM_945782LOC6447141
NM_022828YTHDC21
NM_016072GOLT1B1
NM_024997ATF7IP21
NM_017623CNNM31
NM_199078CNNM31
NM_014181HSPC1591
NM_017637BNC21
NM_025182KIAA15391
NM_145754KIFC21
NM_002973ATXN21
NM_000742CHRNA21
NM_003396WNT9B1
NM_032457PCDH71
NM_001002862DERL31
NM_198440DERL31
NM_173551ANKS61
NM_001676ATP12A1
NM_003734AOC31
NM_001002262ZFYVE271
NM_001002261ZFYVE271
NM_144588ZFYVE271
NM_006494ERF1
NM_0010985121
NM_006258PRKG11
XM_001131019LOC7318461
XM_001131201LOC7297511
NM_213594RFX41
NM_032491RFX41
NM_020313CIAPIN11
NM_015568PPP1R16B1
NM_053042KIAA17291
NM_003190TAPBP1
NM_001012763GNRHR1
NM_000406GNRHR1
NM_198086JUB1
NM_032876JUB1
NM_000831GRIK31
NM_005598NHLH11
NM_024896KIAA18151
NM_014553TFCP2L11
NM_016929CLIC51
NM_003644GAS71
NM_201432GAS71
NM_201433GAS71
NM_003693SCARF11
NM_145351SCARF11
NM_145350SCARF11
NM_145349SCARF11
NM_145352SCARF11
NM_024674LIN281
NM_015046SETX1
NM_005797EVA11
NM_015602TOR1AIP11
NM_006187OAS31
NM_002072GNAQ1
NM_032289PSD21
NM_012471TRPC51
NM_144580C1orf851
NM_001025436SPAG161
NM_014089NUPL11
NM_001008564NUPL11
NM_0011099741
NM_033437PDE5A1
NM_001083PDE5A1
NM_033430PDE5A1
NM_023009MARCKSL11
NM_018045BSDC11
NM_175861TMTC11
NM_145168HSPC1051
NM_0010854601
NM_0010854691
NM_0010854651
NM_0010854661
NM_0010854621
NM_0010854611
NM_0010854581
NM_0010854681
NM_0010854631
NM_0010854671
NM_001331CTNND11
NM_0010854591
NM_0010854641
NM_015077SARM11
NM_023942LRRC611
NM_001076674TMUB21
NM_177441TMUB21
NM_024107TMUB21
NM_138636TLR81
NM_194286KIAA18531
NM_016173HEMK11
NM_004145MYO9B1
NM_004959NR5A11
NM_015655ZNF3371
NM_000555DCX1
NM_178152DCX1
NM_178153DCX1
NM_178151DCX1
NM_002486NCBP11
NM_014653WSCD21
NM_013396USP251
NM_002718PPP2R3A1
NM_181897PPP2R3A1
NM_033087ALG21
NM_0010992701
NM_005765ATP6AP21
NM_024922CES31
NM_145255MRPL101
NM_148887MRPL101
NM_003310TSSC11
NM_172375KCNH51
NM_152996ST6GALNAC31
NM_012416RANBP61
XM_001127362KIAA05741
NM_152597FSIP11
NM_018363C10orf591
NM_152557ZNF7461
NM_152624DCP21
NM_173666DTWD21
NM_006278ST3GAL41
XM_940335PTPRQ1
XM_001134446PTPRQ1
NM_006566CD2261
NM_033133CNP1
NM_003298NR2C21
XM_943832MIA31
XM_932515MIA31
NM_006948STCH1
NM_138638CFL21
NM_021914CFL21
NM_177457LYNX11
NM_177477LYNX11
NM_177476LYNX11
NM_001001852PIM31
XM_938171PIM31
XM_001127568LOC7284991
XM_001127665LOC7285261
XM_001130837LOC6433671
XM_001130826LOC7284521
XM_932646LOC3400891
XM_001127715LOC7285401
XM_001127825LOC7285751
XM_001128001LOC7284011
XM_001127991LOC7284011
NM_001079526IKZF21
NM_016260IKZF21
NM_133647SLC12A61
NM_001042495SLC12A61
NM_001042494SLC12A61
NM_001042497SLC12A61
NM_005135SLC12A61
NM_001042496SLC12A61
NM_022840METTL41
NM_004665VNN21
NM_078488VNN21
NM_080626BRI3BP1
NM_001001702FLJ333601
XM_940667SDC31
NM_004285H6PD1
NM_0010994341
NM_005614RHEB1
NM_003768PEA151
NM_018584CAMK2N11
NM_018425PI4KII1
NM_003980MAP71
NM_001007176C8orf221
NM_002688SEPT51
NM_012287CENTB21
NM_199040NUDT41
NM_019094NUDT41
NM_152389CCDC1081
NM_001039547GK51
NM_014253ODZ11
NM_005955MTF11
NM_020385REXO41
NM_145176SLC2A121
NM_0011045461
XM_001133503LOC6450271
XM_944835LOC6486911
NM_018036C14orf1031
NM_001798CDK21
NM_052827CDK21
NM_025232REEP41
NM_022918TMEM1351
NM_020734FAM80B1
NM_052966FAM129A1
NM_020338ZMIZ11
NM_002737PRKCA1
NM_020390EIF5A21
NM_014331SLC7A111
NM_0010992941
XM_936510KIAA16441
NM_006076HRBL1
NM_002755MAP2K11
NM_019072SGTB1
NM_012069ATP1B41
NM_139321ATRN1
NM_020432PHTF21
XM_930823LOC6423351
XM_943378LOC6423351
NM_004375COX111
NM_014805EPM2AIP11
NM_152495CNIH31
NM_007006NUDT211
XM_943323LOC6467191
XM_933842LOC6467191
NM_173502PRSS361
NM_014779TSC22D21
NM_001083591CUGBP21
NM_001025077CUGBP21
NM_006561CUGBP21
NM_001025076CUGBP21
NM_030626LRRC271
NM_000429MAT1A1
XM_937979MGC463361
XM_290712MGC463361
NM_012239SIRT31
NM_001017524SIRT31
NM_198149TMEM581
NM_002350LYN1
NM_0011110971
NM_022716PRRX11
NM_006902PRRX11
NM_015039NMNAT21
NM_170706NMNAT21
NM_0178921
NM_020456C13orf11
NM_016206VGLL31
NM_207406CCDC41
NM_052861MGC216751
NM_032510PARD6G1
NM_021183RAP2C1
NM_006892DNMT3B1
NM_175848DNMT3B1
NM_175849DNMT3B1
NM_175850DNMT3B1
NM_001010903DKFZp779B15401
NM_006873STON11
NM_0011127041

Immunostimulatory motif search results


UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).

GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).



Settings used for search

Scores were calculated using scoring scheme SS1
Using AVG scoring mode.

Hit scores

ParameterSpec. Score cut-offColourComment
Hit to same geneColourHits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit.
High scoring hit≥80ColourHits are very likely to cause an off-target effect
Medium scoring hit<80¸≥40ColourHits with a small chance of causing an off target effect.
Low scoring hitColourHits very unlikely to cause an off-target effect

The mismatches are colour coded based on the weighting of that position.

Cut-offColour
MatchColour
Wobble base pairs (G-U)Colour
Critical bases (none)Colour
0.6 bitsColour
0.4 bitsColour
0.2 bitsColour

About SpecificityServer

SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).

Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer


For more details about the algorithm "siRNA specificity searching incorporating mismatch tolerance data" Bioinformatics (2008) 24 (10): 1316-1317