Automatic recommendation
Not OK - siRNA is not specific
118 hits were found for GAGCUGGACAAGUACUUCA (110 guide strand hits and 8 sense strand hits.)
Hits to Guide strand
Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
XR_018179 | LOC642448 | 911 | 929 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | U | g | u | 92 | 2 | 17 | ac | false | <90% | 1408 | ||
XR_016161 | LOC642448 | 1088 | 1106 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | U | g | u | 92 | 2 | 17 | ac | false | <90% | 1585 | ||
NM_030964 | SPRY4 | 707 | 725 | G | A | G | C | U | G | G | A | C | A | A | G | c | A | C | U | U | C | u | 84 | 2 | 12 | false | <90% | 4934 | |||
XR_018291 | LOC648333 | 358 | 376 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 857 | ||
XR_017011 | LOC646292 | 358 | 376 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 857 | ||
XR_017035 | LOC442367 | 650 | 668 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1130 | ||
XR_018311 | LOC139060 | 846 | 864 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1163 | ||
XR_016367 | LOC139060 | 846 | 864 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1163 | ||
XR_016342 | LOC391458 | 789 | 807 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1279 | ||
XR_018662 | LOC391458 | 789 | 807 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1279 | ||
XR_016544 | LOC442252 | 788 | 806 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1283 | ||
XR_019169 | LOC442252 | 788 | 806 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1283 | ||
XR_017034 | LOC342374 | 794 | 812 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1291 | ||
XR_018166 | LOC342374 | 794 | 812 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1291 | ||
XR_016362 | LOC390634 | 777 | 795 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1292 | ||
XR_018618 | LOC390634 | 777 | 795 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1292 | ||
XR_019295 | LOC119722 | 808 | 826 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | c | g | u | 68 | 3 | 16 | ac | false | <90% | 1304 | ||
XR_017109 | LOC119722 | 808 | 826 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | c | g | u | 68 | 3 | 16 | ac | false | <90% | 1304 | ||
XR_018819 | LOC650134 | 800 | 818 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1345 | ||
XR_016516 | LOC344866 | 800 | 818 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1346 | ||
XR_018843 | LOC649233 | 850 | 868 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1349 | ||
XR_016443 | LOC643858 | 850 | 868 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1349 | ||
XR_017012 | LOC391179 | 867 | 885 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1425 | ||
XR_018953 | LOC391179 | 867 | 885 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1425 | ||
XR_015721 | LOC732105 | 973 | 991 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | a | g | u | 68 | 3 | 16 | ac | false | <90% | 1463 | ||
XR_019231 | LOC442249 | 983 | 1001 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1480 | ||
XR_016871 | LOC442249 | 983 | 1001 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1480 | ||
XR_016695 | LOC345430 | 1009 | 1027 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | a | g | u | 68 | 3 | 16 | ac | false | <90% | 1499 | ||
XR_018792 | LOC441133 | 1009 | 1027 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1516 | ||
XR_016780 | LOC441133 | 1009 | 1027 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1516 | ||
XR_015648 | LOC731907 | 1037 | 1055 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1606 | ||
XR_015480 | LOC729211 | 1037 | 1055 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1606 | ||
XR_018906 | LOC399965 | 1205 | 1223 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1705 | ||
XR_016979 | LOC399965 | 1205 | 1223 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1705 | ||
XR_015386 | LOC728300 | 1271 | 1289 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1834 | ||
XR_015604 | LOC730933 | 1271 | 1289 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 68 | 3 | 16 | ac | false | <90% | 1835 | ||
XR_016173 | LOC390418 | 806 | 824 | a | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 66 | 4 | 15 | ac | false | <90% | 1302 | ||
XR_018131 | LOC647511 | 806 | 824 | a | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 66 | 4 | 15 | ac | false | <90% | 1302 | ||
XR_019060 | LOC644030 | 860 | 878 | a | A | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 66 | 4 | 15 | ac | false | <90% | 1358 | ||
NM_005550 | KIFC3 | 1668 | 1686 | G | A | G | C | U | G | G | A | C | A | A | G | g | u | C | U | U | C | u | 65 | 3 | 12 | false | <90% | 3366 | |||
XR_017360 | LOC391589 | 776 | 794 | G | u | G | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 57 | 4 | 14 | ac | false | <90% | 1363 | ||
NM_006680 | ME3 | 409 | 427 | G | A | c | C | U | G | G | A | C | A | A | G | U | A | C | a | U | C | A | 54 | 2 | 12 | c | false | <90% | 2090 | ||
NM_001014811 | ME3 | 561 | 579 | G | A | c | C | U | G | G | A | C | A | A | G | U | A | C | a | U | C | A | 54 | 2 | 12 | c | false | <90% | 2242 | ||
XR_018938 | LOC132391 | 775 | 793 | G | A | c | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 49 | 4 | 13 | c | false | <90% | 1272 | ||
XR_016845 | LOC132391 | 775 | 793 | G | A | c | C | U | G | G | A | C | A | A | G | U | A | C | U | g | g | u | 49 | 4 | 13 | c | false | <90% | 1272 | ||
XR_016765 | LOC643471 | 778 | 796 | G | A | G | C | U | G | G | A | C | A | A | G | U | g | C | U | g | g | u | 49 | 4 | 13 | c | false | <90% | 1277 | ||
XR_018670 | LOC643471 | 778 | 796 | G | A | G | C | U | G | G | A | C | A | A | G | U | g | C | U | g | g | u | 49 | 4 | 13 | c | false | <90% | 1277 | ||
XR_015439 | LOC729050 | 848 | 866 | G | A | G | C | U | G | G | A | C | A | A | G | U | g | C | U | g | g | u | 49 | 4 | 13 | c | false | <90% | 1434 | ||
XR_015605 | LOC731794 | 848 | 866 | G | A | G | C | U | G | G | A | C | A | A | G | U | g | C | U | g | g | u | 49 | 4 | 13 | c | false | <90% | 1434 | ||
NM_003204 | NFE2L1 | 809 | 827 | G | A | c | C | U | G | G | A | C | A | A | u | U | A | C | U | U | C | A | 46 | 2 | 8 | false | <90% | 4760 | |||
NM_006258 | PRKG1 | 191 | 209 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | c | g | C | u | 45 | 3 | 15 | c | false | <90% | 3740 | ||
XR_017091 | LOC391703 | 694 | 712 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | u | U | g | g | u | 44 | 4 | 14 | c | false | <90% | 1186 | ||
XR_015309 | LOC730918 | 727 | 745 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | u | U | g | g | u | 44 | 4 | 14 | c | false | <90% | 1219 | ||
XR_016865 | LOC340460 | 794 | 812 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | g | U | g | g | u | 44 | 4 | 14 | c | false | <90% | 1411 | ||
XR_018344 | LOC340460 | 794 | 812 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | g | U | g | g | u | 44 | 4 | 14 | c | false | <90% | 1465 | ||
NM_152263 | TPM3 | 284 | 302 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | u | U | c | u | g | 44 | 4 | 14 | c | false | <90% | 7116 | ||
XR_016590 | LOC392516 | 516 | 534 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | g | g | g | u | 42 | 4 | 15 | c | false | <90% | 1115 | ||
XR_018216 | LOC647913 | 516 | 534 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | g | g | g | u | 42 | 4 | 15 | c | false | <90% | 1115 | ||
XR_017288 | LOC390904 | 844 | 862 | G | A | G | C | U | G | G | A | C | A | A | G | U | A | C | c | a | g | u | 42 | 4 | 15 | c | false | <90% | 1403 | ||
XR_016329 | LOC391271 | 723 | 741 | G | A | G | C | U | G | G | A | C | A | A | a | U | A | C | U | a | g | u | 35 | 4 | 11 | false | <90% | 1219 | |||
XR_019037 | LOC391271 | 723 | 741 | G | A | G | C | U | G | G | A | C | A | A | a | U | A | C | U | a | g | u | 35 | 4 | 11 | false | <90% | 1219 | |||
XR_016525 | LOC121054 | 783 | 801 | G | A | G | C | U | G | G | A | C | A | A | a | U | A | C | U | g | g | u | 35 | 4 | 11 | false | <90% | 1279 | |||
XR_019191 | LOC121054 | 783 | 801 | G | A | G | C | U | G | G | A | C | A | A | a | U | A | C | U | g | g | u | 35 | 4 | 11 | false | <90% | 1279 | |||
XR_016517 | LOC392371 | 872 | 890 | G | A | G | C | U | G | G | A | C | A | A | a | U | A | C | U | g | g | u | 35 | 4 | 11 | false | <90% | 1367 | |||
XR_018983 | LOC392371 | 872 | 890 | G | A | G | C | U | G | G | A | C | A | A | a | U | A | C | U | g | g | u | 35 | 4 | 11 | false | <90% | 1367 | |||
NM_032432 | ABLIM2 | 234 | 252 | G | u | G | C | a | G | G | A | C | A | A | G | U | A | C | U | U | C | c | 32 | 3 | 13 | bc | false | <90% | 3416 | ||
NM_017550 | MIER2 | 226 | 244 | G | A | G | C | U | G | G | A | g | A | A | G | g | A | C | U | U | C | A | 29 | 2 | 8 | false | <90% | 2825 | |||
NM_172016 | TRIM39 | 1440 | 1458 | G | A | G | C | U | G | G | A | a | A | A | G | a | A | C | U | U | C | A | 29 | 2 | 8 | false | <90% | 3545 | |||
NM_021253 | TRIM39 | 1530 | 1548 | G | A | G | C | U | G | G | A | a | A | A | G | a | A | C | U | U | C | A | 29 | 2 | 8 | false | <90% | 3635 | |||
NM_001348 | DAPK3 | 829 | 847 | G | A | G | C | U | G | G | c | C | A | A | G | g | A | C | U | U | C | A | 27 | 2 | 7 | false | <90% | 2105 | |||
XR_018187 | LOC647722 | 433 | 451 | c | A | G | C | U | G | G | A | g | A | A | G | g | A | C | U | U | C | A | 27 | 3 | 7 | false | <90% | 1213 | |||
XR_017039 | LOC645798 | 433 | 451 | c | A | G | C | U | G | G | A | g | A | A | G | g | A | C | U | U | C | A | 27 | 3 | 7 | false | <90% | 1213 | |||
XR_016530 | LOC643220 | 433 | 451 | c | A | G | C | U | G | G | A | g | A | A | G | g | A | C | U | U | C | A | 27 | 3 | 7 | false | <90% | 1256 | |||
XR_019077 | LOC642741 | 459 | 477 | c | A | G | C | U | G | G | A | g | A | A | G | g | A | C | U | U | C | A | 27 | 3 | 7 | false | <90% | 1310 | |||
XR_016455 | LOC642741 | 459 | 477 | c | A | G | C | U | G | G | A | g | A | A | G | g | A | C | U | U | C | A | 27 | 3 | 7 | false | <90% | 1310 | |||
NM_000967 | RPL3 | 501 | 519 | c | A | G | C | U | G | G | A | g | A | A | G | g | A | C | U | U | C | A | 27 | 3 | 7 | false | <90% | 1348 | |||
NM_005049 | PWP2 | 916 | 934 | c | g | G | C | U | G | G | c | C | A | A | G | U | A | C | U | U | C | u | 19 | 4 | 10 | bc | false | <90% | 3277 | ||
NM_013261 | PPARGC1A | 1501 | 1519 | G | A | G | C | U | G | a | A | C | A | A | G | c | A | C | U | U | C | g | 18 | 3 | 6 | false | <90% | 6318 | |||
NM_004566 | PFKFB3 | 1374 | 1392 | G | A | G | C | a | G | G | A | C | A | A | G | U | A | C | U | a | u | u | 17 | 4 | 11 | c | false | <90% | 4497 | ||
NM_015267 | CUTL2 | 3289 | 3307 | G | A | G | C | U | G | G | A | C | A | c | G | U | A | C | U | c | C | A | 15 | 2 | 10 | c | false | <90% | 6841 | ||
NM_006901 | MYO9A | 1063 | 1081 | G | A | G | C | U | G | G | A | C | c | A | G | U | A | C | U | U | g | A | 14 | 2 | 9 | c | false | <90% | 8473 | ||
NM_014676 | PUM1 | 3600 | 3618 | a | A | G | C | U | G | G | A | g | A | A | G | U | A | C | U | a | C | A | 14 | 3 | 7 | c | false | <90% | 5410 | ||
NM_001020658 | PUM1 | 3606 | 3624 | a | A | G | C | U | G | G | A | g | A | A | G | U | A | C | U | a | C | A | 14 | 3 | 7 | c | false | <90% | 5416 | ||
NM_001048 | SST | 261 | 279 | G | A | a | C | U | G | G | c | C | A | A | G | U | A | C | U | U | C | u | 13 | 3 | 10 | bc | false | <90% | 665 | ||
NM_145268 | C7orf45 | 243 | 261 | c | A | G | u | g | G | G | A | C | A | A | G | U | A | C | U | U | C | A | 9 | 3 | 14 | bc | false | <90% | 1953 | ||
XR_019625 | LOC391256 | 795 | 813 | G | A | G | C | U | G | G | A | C | A | u | G | U | A | C | U | g | a | u | 7 | 4 | 10 | c | false | <90% | 1346 | ||
XR_016575 | LOC391256 | 797 | 815 | G | A | G | C | U | G | G | A | C | A | u | G | U | A | C | U | g | a | u | 7 | 4 | 10 | c | false | <90% | 1348 | ||
XR_016450 | LOC139748 | 993 | 1011 | G | A | G | C | U | G | G | A | C | A | u | G | U | A | C | U | g | g | u | 7 | 4 | 10 | c | false | <90% | 1489 | ||
XR_018559 | LOC649375 | 993 | 1011 | G | A | G | C | U | G | G | A | C | A | u | G | U | A | C | U | g | g | u | 7 | 4 | 10 | c | false | <90% | 1489 | ||
XR_019568 | LOC343326 | 1099 | 1117 | G | A | G | C | U | G | G | g | C | A | A | G | U | A | C | U | g | g | u | 6 | 4 | 8 | c | false | <90% | 1596 | ||
XR_017689 | LOC343326 | 1099 | 1117 | G | A | G | C | U | G | G | g | C | A | A | G | U | A | C | U | g | g | u | 6 | 4 | 8 | c | false | <90% | 1596 | ||
XR_017456 | LOC647162 | 1158 | 1176 | G | A | G | C | U | G | G | g | C | A | A | G | U | A | C | U | g | g | u | 6 | 4 | 8 | c | false | <90% | 1637 | ||
XR_019330 | LOC652370 | 1158 | 1176 | G | A | G | C | U | G | G | g | C | A | A | G | U | A | C | U | g | g | u | 6 | 4 | 8 | c | false | <90% | 1637 | ||
NM_173624 | FLJ40504 | 1387 | 1405 | G | A | G | C | U | G | G | g | C | A | A | G | U | A | C | U | g | g | u | 6 | 4 | 8 | c | false | <90% | 1950 | ||
XR_017241 | LOC646723 | 793 | 811 | G | A | G | C | U | a | G | A | C | A | A | G | U | A | C | U | g | g | u | 5 | 4 | 10 | c | false | <90% | 1292 | ||
XR_018339 | LOC648448 | 793 | 811 | G | A | G | C | U | a | G | A | C | A | A | G | U | A | C | U | g | g | u | 5 | 4 | 10 | c | false | <90% | 1292 | ||
XR_019026 | LOC339781 | 780 | 798 | G | A | G | C | U | u | G | A | C | A | A | G | U | A | C | U | g | g | u | 5 | 4 | 10 | c | false | <90% | 1333 | ||
XR_016833 | LOC339781 | 780 | 798 | G | A | G | C | U | u | G | A | C | A | A | G | U | A | C | U | g | g | u | 5 | 4 | 10 | c | false | <90% | 1333 | ||
NM_199187 | KRT18 | 865 | 883 | G | A | G | C | U | a | G | A | C | A | A | G | U | A | C | U | g | g | u | 5 | 4 | 10 | c | false | <90% | 1439 | ||
NM_000224 | KRT18 | 911 | 929 | G | A | G | C | U | a | G | A | C | A | A | G | U | A | C | U | g | g | u | 5 | 4 | 10 | c | false | <90% | 1485 | ||
XR_018653 | LOC643329 | 761 | 779 | G | A | G | C | U | a | G | A | C | A | A | G | U | A | C | U | g | g | u | 5 | 4 | 10 | c | false | <90% | 1892 | ||
XR_016332 | LOC643329 | 761 | 779 | G | A | G | C | U | a | G | A | C | A | A | G | U | A | C | U | g | g | u | 5 | 4 | 10 | c | false | <90% | 2003 | ||
XR_018462 | LOC391803 | 770 | 788 | G | A | G | C | U | G | a | A | C | A | A | G | U | A | C | U | g | g | u | 2 | 4 | 9 | c | false | <90% | 1234 | ||
XR_016739 | LOC391803 | 770 | 788 | G | A | G | C | U | G | a | A | C | A | A | G | U | A | C | U | g | g | u | 2 | 4 | 9 | c | false | <90% | 1234 | ||
XR_015662 | LOC731954 | 547 | 565 | G | A | G | C | U | G | G | A | C | g | A | G | g | A | C | U | U | C | g | 1 | 3 | 9 | false | <90% | 3402 | |||
XR_017086 | LOC645954 | 1165 | 1183 | G | A | G | C | U | G | G | A | C | g | A | G | g | A | C | U | U | C | g | 1 | 3 | 9 | false | <90% | 4019 | |||
NM_213655 | HSN2 | 392 | 410 | G | A | c | C | U | G | G | A | C | u | A | G | U | A | C | U | U | C | c | -7 | 3 | 8 | bc | false | <90% | 1305 | ||
NM_021035 | ZNFX1 | 3662 | 3680 | G | A | G | C | U | G | u | g | C | A | A | G | U | A | C | U | U | C | c | -33 | 3 | 10 | bc | false | <90% | 7371 | ||
NM_001079812 | DIAPH1 | 3404 | 3422 | G | A | G | C | U | G | G | g | C | g | A | G | U | A | C | U | U | C | c | -49 | 3 | 8 | bc | false | <90% | 5718 | ||
NM_005219 | DIAPH1 | 3431 | 3449 | G | A | G | C | U | G | G | g | C | g | A | G | U | A | C | U | U | C | c | -49 | 3 | 8 | bc | false | <90% | 5745 |
Hits to Sense strand
Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
NM_033285 | TP53INP1 | 1925 | 1943 | g | G | A | A | G | U | A | C | U | U | G | U | C | C | A | G | g | c | a | 66 | 4 | 15 | ac | false | <90% | 5587 | ||
NM_014965 | TRAK1 | 933 | 951 | U | G | A | A | G | a | A | C | U | U | G | U | C | C | A | G | C | a | u | 29 | 3 | 11 | c | false | <90% | 4630 | ||
NM_001042646 | TRAK1 | 1130 | 1148 | U | G | A | A | G | a | A | C | U | U | G | U | C | C | A | G | C | a | u | 29 | 3 | 11 | c | false | <90% | 5123 | ||
NM_004445 | EPHB6 | 3135 | 3153 | U | G | c | A | G | U | A | C | c | U | G | U | C | C | A | G | C | U | u | 15 | 3 | 9 | bc | false | <90% | 4059 | ||
NM_014289 | CAPN6 | 1633 | 1651 | U | G | A | A | G | U | g | C | c | U | G | U | C | C | A | G | C | U | C | -26 | 2 | 10 | bc | false | <90% | 3568 | ||
NM_052946 | NOSTRIN | 405 | 423 | U | G | A | A | G | c | A | C | U | U | u | U | C | C | A | G | C | U | u | -29 | 3 | 7 | c | false | <90% | 1588 | ||
NM_001039724 | NOSTRIN | 445 | 463 | U | G | A | A | G | c | A | C | U | U | u | U | C | C | A | G | C | U | u | -29 | 3 | 7 | c | false | <90% | 1634 | ||
NM_004521 | KIF5B | 2317 | 2335 | U | G | A | A | G | a | A | C | U | a | G | U | C | C | A | G | C | U | u | -51 | 3 | 8 | bc | false | <90% | 3688 |
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 3205
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
Parameter | Spec. Score cut-off | Colour | Comment |
Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
Cut-off | Colour |
Match | Colour |
Wobble base pairs (G-U) | Colour |
Critical bases (none) | Colour |
0.6 bits | Colour |
0.4 bits | Colour |
0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer