Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands
12 hits were found for UGGCCGGAGUCCUUUAAGA (10 guide strand hits and 2 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_004594 | SLC9A5 | 1993 | 2011 | c | G | G | C | u | G | G | A | G | U | C | C | U | U | U | A | A | G | u | 41 | 3 | 13 | bc | false | <90% | 3745 | ||
| NM_024675 | PALB2 | 507 | 525 | g | G | G | C | C | u | G | A | G | U | C | C | U | U | U | A | A | c | c | 27 | 4 | 11 | c | false | <90% | 4069 | ||
| NM_199450 | ZNF365 | 314 | 332 | U | G | G | C | a | G | G | A | G | U | C | C | U | U | U | g | A | G | A | 22 | 2 | 10 | c | false | <90% | 1671 | ||
| NM_199451 | ZNF365 | 314 | 332 | U | G | G | C | a | G | G | A | G | U | C | C | U | U | U | g | A | G | A | 22 | 2 | 10 | c | false | <90% | 3376 | ||
| NM_014951 | ZNF365 | 314 | 332 | U | G | G | C | a | G | G | A | G | U | C | C | U | U | U | g | A | G | A | 22 | 2 | 10 | c | false | <90% | 4158 | ||
| NM_052851 | STARD13 | 899 | 917 | g | a | G | C | C | a | G | A | G | U | C | C | U | U | U | A | A | G | g | 18 | 4 | 12 | bc | false | <90% | 5784 | ||
| NM_178007 | STARD13 | 925 | 943 | g | a | G | C | C | a | G | A | G | U | C | C | U | U | U | A | A | G | g | 18 | 4 | 12 | bc | false | <90% | 5810 | ||
| NM_178006 | STARD13 | 999 | 1017 | g | a | G | C | C | a | G | A | G | U | C | C | U | U | U | A | A | G | g | 18 | 4 | 12 | bc | false | <90% | 5884 | ||
| XM_378914 | KIAA0492 | 793 | 811 | U | G | G | C | C | G | G | c | G | U | C | C | U | U | U | A | g | G | c | 9 | 3 | 8 | c | false | <90% | 6259 | ||
| XM_944290 | KIAA0492 | 793 | 811 | U | G | G | C | C | G | G | c | G | U | C | C | U | U | U | A | g | G | c | 9 | 3 | 8 | c | false | <90% | 6260 | ||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| XM_046531 | KIAA1614 | 1094 | 1112 | c | C | U | U | g | c | A | G | G | A | C | U | C | C | G | G | C | C | A | -16 | 3 | 13 | bc | false | <90% | 6999 | ||
| XM_942569 | KIAA1614 | 1094 | 1112 | c | C | U | U | g | c | A | G | G | A | C | U | C | C | G | G | C | C | A | -16 | 3 | 13 | bc | false | <90% | 7000 | ||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 894
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer