VIRsiRNAdb

SpecificityServer - An siRNA design program for determining siRNA specificity
Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands

31 hits were found for CUCGUCUUCCACUGCCAGC (13 guide strand hits and 18 sense strand hits.)

Hits to Guide strand

Accession

Gene

Start

End

Details of mismatches

Spec. Score

Mismatches

Contiguous

Jackson style specificity

Hits similar gene

HSP Score

HSP % Identity

HSP length

Gene Length

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

NM_144691

CAPN12

718

736

g

g

C

G

U

C

U

U

C

C

A

C

U

u

C

C

A

G

C

67

3

11

c

false

<90%

3129

NM_004411

DYNC1I1

1189

1207

u

a

C

G

U

C

U

U

C

C

A

C

U

G

u

C

A

G

u

56

4

12

c

false

<90%

2942

XM_938374

LOC644010

2319

2337

a

c

C

G

U

C

U

U

C

C

A

C

U

G

C

a

A

G

a

55

4

13

c

false

<90%

3939

NM_033068

ACPT

653

671

a

c

g

G

U

C

U

U

C

C

A

C

U

a

C

C

A

G

C

47

4

10

c

false

<90%

1347

XM_001131876

PRM3

361

379

g

U

a

c

U

C

U

U

C

C

A

C

U

G

C

C

A

G

u

36

4

14

bc

false

<90%

474

NM_020162

DHX33

1971

1989

g

U

C

c

U

C

U

U

C

C

A

C

U

G

C

a

A

G

C

35

3

11

c

false

<90%

4075

NM_024095

ASB8

984

1002

a

g

g

G

c

C

U

U

C

C

A

C

U

G

C

C

A

G

C

15

4

14

bc

false

<90%

2629

NM_004176

SREBF1

1671

1689

C

U

C

G

U

C

U

U

C

C

u

C

U

G

C

C

u

G

u

10

3

10

c

false

<90%

4194

NM_001005291

SREBF1

1761

1779

C

U

C

G

U

C

U

U

C

C

u

C

U

G

C

C

u

G

u

10

3

10

c

false

<90%

4284

NM_001080410

FBXO41

6589

6607

C

U

C

c

U

C

U

g

C

C

A

C

U

G

C

C

A

G

C

1

2

11

bc

false

<90%

7121

XM_001133261

FBXO41

6589

6607

C

U

C

c

U

C

U

g

C

C

A

C

U

G

C

C

A

G

C

1

2

11

bc

false

<90%

7121

NM_001171

ABCC6

2877

2895

u

U

C

c

U

C

U

U

C

C

u

C

U

G

C

C

A

G

C

0

3

8

c

false

<90%

5111

NM_014431

KIAA1274

1201

1219

C

U

C

G

U

C

U

U

C

a

g

C

U

G

C

C

A

G

a

-49

3

9

c

false

<90%

4569

Hits to Sense strand

Accession

Gene

Start

End

Details of mismatches

Spec. Score

Mismatches

Contiguous

Jackson style specificity

Hits similar gene

HSP Score

HSP % Identity

HSP length

Gene Length

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

NM_020131

UBQLN4

414

432

G

C

U

G

G

C

A

G

U

G

G

A

A

G

c

C

G

g

a

67

3

14

c

false

<90%

3545

NM_003763

STX16

98

116

G

C

U

G

G

C

A

G

U

G

G

A

g

G

A

C

c

A

G

66

2

12

false

<90%

4896

NM_001001433

STX16

98

116

G

C

U

G

G

C

A

G

U

G

G

A

g

G

A

C

c

A

G

66

2

12

false

<90%

4959

NM_002924

RGS7

1417

1435

G

C

U

G

G

C

A

G

U

G

G

A

g

G

A

C

c

u

G

63

3

12

false

<90%

2402

NM_144612

LOXHD1

2688

2706

G

C

U

G

G

C

A

G

U

G

G

A

g

G

A

a

G

A

u

58

3

12

false

<90%

4775

NM_024761

MOBKL2B

397

415

G

C

U

G

G

C

A

G

U

G

G

A

A

a

A

C

a

c

u

49

4

13

c

false

<90%

6528

XM_001131196

LOC646425

817

835

G

C

U

G

G

C

A

G

U

G

G

A

A

G

u

C

a

A

a

46

3

14

c

false

<90%

1806

XM_942022

LOC646425

817

835

G

C

U

G

G

C

A

G

U

G

G

A

A

G

u

C

a

A

a

46

3

14

c

false

<90%

1806

NM_138793

CANT1

1083

1101

G

C

U

G

G

C

A

G

U

G

a

A

g

G

A

C

G

A

G

28

2

10

false

<90%

3473

NM_003637

ITGA10

1318

1336

a

a

U

G

G

C

A

c

U

G

G

A

A

G

A

C

G

A

G

24

3

11

bc

false

<90%

5189

NM_133370

YTHDC1

3188

3206

c

g

U

G

G

C

A

G

c

G

G

A

A

G

A

C

G

A

u

21

4

9

bc

false

<90%

6195

NM_001031732

YTHDC1

3242

3260

c

g

U

G

G

C

A

G

c

G

G

A

A

G

A

C

G

A

u

21

4

9

bc

false

<90%

6249

NM_006305

ANP32A

684

702

u

C

a

G

G

u

A

G

U

G

G

A

A

G

A

C

G

A

G

15

3

13

bc

false

<90%

1136

NM_015148

PASK

2033

2051

G

C

U

G

G

g

A

G

U

G

G

A

A

a

A

C

G

A

c

12

3

7

c

false

<90%

4530

NM_004557

NOTCH4

5267

5285

G

C

U

G

G

C

g

G

U

G

G

A

A

G

A

C

c

u

G

7

3

9

c

false

<90%

6762

NM_001039589

DPH2

926

944

G

C

U

G

u

g

A

G

U

G

G

A

A

G

A

C

G

A

G

-14

2

13

bc

false

<90%

1829

NM_001384

DPH2

1610

1628

G

C

U

G

u

g

A

G

U

G

G

A

A

G

A

C

G

A

G

-14

2

13

bc

false

<90%

2513

NM_020884

MYH7B

4965

4983

G

C

U

G

G

C

A

G

a

G

a

A

A

G

A

C

G

A

G

-21

2

8

c

false

<90%

6289

---

3 prime UTR Seed matches

Hits to same gene : 0
Hits to other genes : 545
View matched accessions

3 prime seed match details

Accession	Gene name	Hits to this gene
NM_173627	FLJ35220	5
NM_020911		2
NM_005686	SOX13	2
NM_018999	KIAA1128	2
NM_152362	TNFAIP8L1	2
NM_198461	LONRF2	2
NM_005188	CBL	2
NM_001010980	C1orf130	2
NM_032817	ITIH5	2
NM_030569	ITIH5	2
NM_014974	DIP2C	2
NM_032173		2
NM_014777	KIAA0133	2
NM_014964	EPN2	2
NM_001102664		2
NM_148921	EPN2	2
NM_147202	C9orf25	2
NM_030806	C1orf21	2
NM_024591	CHMP6	2
XM_001132548	CHMP6	2
NM_014957	DENND3	2
NM_198076	FAM36A	1
NM_001010888	ZC3H12B	1
NM_006042	HS3ST3A1	1
NM_004098	EMX2	1
NM_013435	RAX	1
NM_001017924	LRRC22	1
NM_015330	SPECC1L	1
NM_052917	GALNT13	1
NM_006620	HBS1L	1
NM_031438	NUDT12	1
NM_182487	OLFML2A	1
XM_378399	LOC400099	1
XM_944233	LOC400099	1
NM_138328	RHBDL3	1
NM_207015	NAALADL2	1
NM_015678	NBEA	1
NM_002698	POU2F2	1
NM_015188		1
NM_004758	BZRAP1	1
NM_176815	DHFRL1	1
NM_012192	FXC1	1
NM_033034	TRIM5	1
NM_033093	TRIM5	1
NM_021945	C6orf85	1
NM_002581	PAPPA	1
NM_015348		1
NM_031448	C19orf12	1
NM_001031726	C19orf12	1
NM_000629	IFNAR1	1
XM_001125898	LOC727762	1
NM_139076	CCDC98	1
NM_022767	ISG20L1	1
NM_001287	CLCN7	1
NM_032564	DGAT2	1
NM_006464	TGOLN2	1
NM_183397	PXMP4	1
NM_052876	BTBD14B	1
NM_178500	PHOSPHO1	1
NM_001860	SLC31A2	1
NM_152833		1
NM_178836	LOC201164	1
NM_017759	FLJ20309	1
NM_025000	C2orf37	1
NM_006359	SLC9A6	1
NM_001042537	SLC9A6	1
NM_000415	IAPP	1
NM_014186	COMMD9	1
NM_001101653		1
NM_001025108	AFF3	1
NM_002285	AFF3	1
NM_001039884	FLJ44894	1
NM_052925	LENG8	1
NM_004984	KIF5A	1
NM_153685	C12orf53	1
XM_001129349	LOC728346	1
NM_021195	CLDN6	1
NM_003957	BRSK2	1
NM_172388	NFATC1	1
NM_006162	NFATC1	1
NM_172389	NFATC1	1
NM_172387	NFATC1	1
NM_007051	FAF1	1
NM_000692	ALDH1B1	1
NM_001040446	MTMR12	1
NM_015227	POFUT2	1
NM_032493	AP1M1	1
NM_031910	C1QTNF6	1
NM_182486	C1QTNF6	1
NM_003644	GAS7	1
NM_201432	GAS7	1
NM_201433	GAS7	1
NM_018045	BSDC1	1
NM_023007	JMJD4	1
NM_003221	TFAP2B	1
NM_020374	C12orf4	1
NM_014759	PHYHIP	1
NM_001099335		1
NM_024946	NIP30	1
NM_016353	ZDHHC2	1
NM_001031848	SERPINB8	1
NM_152641	ARID2	1
NM_173566	C22orf30	1
NM_025236	RNF39	1
NM_170769	RNF39	1
NM_000587	C7	1
NM_153442	GPR26	1
NM_173485	TSHZ2	1
NM_181725	METTL2A	1
NM_001005372	METTL2A	1
NM_020375	C12orf5	1
NM_005229	ELK1	1
NM_001008223	C1QL4	1
NM_001406	EFNB3	1
NM_016172	UBAC1	1
NM_024949	WWC2	1
NM_001083909	GPR123	1
NM_017588	WDR5	1
NM_052821	WDR5	1
NM_023033	METTL1	1
NM_005371	METTL1	1
NM_139062	CSNK1D	1
NM_000429	MAT1A	1
NM_024649	BBS1	1
NM_181521	CMTM4	1
NM_020971	SPTBN4	1
NM_033386	MICALL1	1
NM_031904	FKSG44	1
NM_005611	RBL2	1
NM_139029	CD151	1
NM_004357	CD151	1
NM_001039490	CD151	1
NM_139030	CD151	1
NM_001388	DRG2	1
NM_002608	PDGFB	1
NM_033016	PDGFB	1
NM_030767	AKNA	1
NM_175609	ARFGAP1	1
NM_018209	ARFGAP1	1
NM_173542	LOC196463	1
NM_005768	MBOAT5	1
NM_001018050	POLR3H	1
NM_138338	POLR3H	1
NM_001018052	POLR3H	1
NM_023075	MPPE1	1
NM_015329	KIAA0892	1
NM_032189	ATP11A	1
NM_015205	ATP11A	1
XM_001133830	LOC729519	1
XM_001134313	LOC732291	1
NM_207397	CD164L2	1
NM_007021	C10orf10	1
NM_014734	KIAA0247	1
XM_001134379	LOC732350	1
XM_001128421	LOC728069	1
NM_001496	GFRA3	1
NM_001080826		1
NM_006257	PRKCQ	1
NM_194071	CREB3L2	1
NM_024046	CAMKV	1
NM_015059	TLN2	1
NM_020804	PACSIN1	1
NM_015553	PIP3-E	1
NM_153029	N4BP1	1
NM_001008528	MXRA7	1
NM_000451	SHOX	1
XM_001131514	LOC729851	1
NM_001001974	PLEKHA1	1
NM_021622	PLEKHA1	1
NM_020708	SLC12A5	1
NM_153718	NUP62	1
NM_012346	NUP62	1
NM_153719	NUP62	1
NM_016553	NUP62	1
NM_021215	C20orf77	1
NM_080836	STK35	1
NM_014839	LPPR4	1
NM_004712	HGS	1
NM_001918	DBT	1
XM_001127604	NFATC2IP	1
NM_032815	NFATC2IP	1
NM_003426	ZNF74	1
NM_153368	CX40.1	1
NM_024719	GRTP1	1
NM_006927	ST3GAL2	1
NM_001044387	ZNF557	1
NM_024341	ZNF557	1
NM_001044388	ZNF557	1
NM_001040441	ZBTB8	1
XM_001124597	LOC730411	1
XM_209227	LOC653464	1
NM_207351	PRRT3	1
NM_003774	GALNT4	1
XM_209363	SIRPB2	1
XM_939897	SIRPB2	1
NM_001099694		1
NM_012400	PLA2G2D	1
NM_003757	EIF3S2	1
NM_020340	KIAA1244	1
NM_144964	RG9MTD3	1
NM_178324	SPTLC1	1
NM_022766	CERK	1
NM_006252	PRKAA2	1
NM_032554	GPR81	1
NM_133646	ZAK	1
NM_022129	PBLD	1
NM_005999	TSNAX	1
XM_932529	LOC644949	1
XM_943887	LOC644949	1
NM_207428	C11orf55	1
NM_020840		1
XM_378661	C17orf51	1
XM_944416	C17orf51	1
NM_000057	BLM	1
NM_004311	ARL3	1
NM_000742	CHRNA2	1
NM_052875	VPS26B	1
NM_004747	DLG5	1
NM_012479	YWHAG	1
NM_133471		1
NM_198484	ZNF621	1
NM_001098414		1
NM_000202	IDS	1
NM_002388	MCM3	1
XM_929980	LOC647000	1
XM_941325	LOC652025	1
NM_017491	WDR1	1
NM_005112	WDR1	1
NM_018401	STK32B	1
NM_020526	EPHA8	1
NM_152730	C6orf170	1
NM_001080498	EMR4	1
NM_016219	MAN1B1	1
NM_005647	TBL1X	1
NM_144570	HN1L	1
NM_004523	KIF11	1
NM_003681	PDXK	1
NM_207445	FLJ39531	1
NM_181784	SPRED2	1
NM_080796	DIDO1	1
NM_022105	DIDO1	1
NM_004265	FADS2	1
NM_001037	SCN1B	1
NM_023067	FOXL2	1
NM_012305	AP2A2	1
NM_004494	HDGF	1
XM_001126895	LOC728219	1
XM_001130447	LOC731691	1
NM_015898	ZBTB7A	1
XM_941540	FLJ90086	1
XM_001134321	LOC732206	1
XM_371820	FLJ90086	1
NM_014023	WDR37	1
NM_000217	KCNA1	1
NM_004976	KCNC1	1
NM_014850	SRGAP3	1
NM_001033117	SRGAP3	1
NM_015569	DNM3	1
NM_000684	ADRB1	1
NM_002134	HMOX2	1
NM_017590	ZC3H7B	1
NM_003576	STK24	1
NM_001032296	STK24	1
NM_207380	FLJ43339	1
NM_003470	USP7	1
NM_006315	PCGF3	1
NM_005653	TFCP2	1
NM_024713	C15orf29	1
NM_198061	CES2	1
NM_003869	CES2	1
NM_020223	FAM20C	1
NM_000332	ATXN1	1
NM_014616	ATP11B	1
NM_020546	ADCY2	1
NM_173500	TTBK2	1
NM_015520	MAGI1	1
NM_001033057	MAGI1	1
NM_152341	PAQR4	1
NM_012146	DUX1	1
NM_152376	UBXD3	1
NM_016529	ATP8A2	1
XM_939547	ZNF697	1
NM_001080470	ZNF697	1
NM_001330	CTF1	1
NM_153360	APCDD1L	1
NM_005186	CAPN1	1
NM_022336	EDAR	1
NM_014747	RIMS3	1
NM_012473	TXN2	1
NM_153332	THEX1	1
NM_022458	LMBR1	1
NM_005883	APC2	1
NM_182595	DKFZp564N2472	1
NM_015270	ADCY6	1
NM_020983	ADCY6	1
NM_002048	GAS1	1
NM_001018071	FRMPD2	1
NM_001017929	FRMPD2	1
NM_001042512	FRMPD2	1
NM_152428	FRMPD2	1
XM_001128149	LOC730316	1
XM_001129924	LOC729316	1
NM_172020	POM121	1
NM_001099415		1
NM_015276		1
NM_017899	TESC	1
NM_020914	RNF213	1
NM_014817	KIAA0644	1
NM_001039577	CCNL2	1
NM_003180	SYT5	1
XM_001126066	LOC730497	1
NM_021646	ZNF500	1
NM_001102402		1
NM_021213	PCTP	1
NM_002862	PYGB	1
XM_001134250	LOC732053	1
XM_001126849	LOC727900	1
NM_022112	P53AIP1	1
NM_005994	TBX2	1
NM_001077657	C3orf43	1
NM_006291	TNFAIP2	1
NM_001010982	AFMID	1
NM_199040	NUDT4	1
NM_019094	NUDT4	1
NM_198990	NAPE-PLD	1
NM_198471	ANKRD47	1
NM_004386	NCAN	1
XM_940069	PPP1R3E	1
XM_927029	PPP1R3E	1
NM_175736	FMNL3	1
NM_198900	FMNL3	1
NM_198148	CPXM2	1
NM_032854	CORO6	1
NM_001032289	SLC35A2	1
NM_001042498	SLC35A2	1
NM_144628	TBC1D20	1
NM_023018	NADK	1
NM_000878	IL2RB	1
NM_052818	CG018	1
NM_001079691	CG018	1
NM_000681	ADRA2A	1
NM_032512	PDZD4	1
NM_152898	FERD3L	1
NM_017870	TMEM132A	1
NM_178031	TMEM132A	1
NM_018361	AGPAT5	1
NM_003458	BSN	1
NM_133474	ZNF721	1
NM_024607	PPP1R3B	1
NM_001017420	ESCO2	1
NM_001077243	GRIA4	1
NM_000829	GRIA4	1
NM_002599	PDE2A	1
NM_138981	MAPK10	1
NM_002753	MAPK10	1
NM_006319	CDIPT	1
NM_000748	CHRNB2	1
NM_002417	MKI67	1
NM_022073	EGLN3	1
NM_001098816		1
NM_005022	PFN1	1
NM_152393	KBTBD5	1
NM_003898	SYNJ2	1
NM_004321	KIF1A	1
XM_001133189	INPP5A	1
NM_005539	INPP5A	1
NM_004524	LLGL2	1
NM_001031803	LLGL2	1
NM_030629	CMIP	1
NM_198390	CMIP	1
NM_031488	L3MBTL2	1
NM_018042	SLFN12	1
NM_017908	ZNF446	1
NM_203327	SLC23A2	1
NM_005116	SLC23A2	1
NM_178540	C1QTNF9	1
NM_012430	SEC22A	1
NM_001018036	TSHR	1
NM_001001412	FAM26C	1
NM_014760	TATDN2	1
NM_014613	UBXD8	1
NM_033120	NKD2	1
NM_001099294		1
XM_936510	KIAA1644	1
XM_001129997	NTSR2	1
NM_012344	NTSR2	1
NM_004090	DUSP3	1
NM_002735	PRKAR1B	1
NM_007118	TRIO	1
NM_007284	TWF2	1
NM_000418	IL4R	1
NM_001009555	SH3D19	1
NM_205849	FAM9B	1
NM_000391	TPP1	1
NM_001040439	MAPK8IP3	1
NM_015133	MAPK8IP3	1
NM_005312	RAPGEF1	1
NM_198679	RAPGEF1	1
NM_017563	IL17RD	1
NM_001080973	IL17RD	1
NM_015855	WIT1	1
NM_032444	BTBD12	1
NM_198481	VSTM1	1
NM_018153	ANTXR1	1
NM_012215	MGEA5	1
NM_000318	PXMP3	1
NM_001079867	PXMP3	1
NM_002126	HLF	1
NM_182501	MTERFD2	1
NM_033529	CDC2L2	1
NM_024011	CDC2L2	1
NM_021203	SRPRB	1
NM_130446	KLHL6	1
NM_031896	CACNG7	1
NM_018937	PCDHB3	1
NM_023942	LRRC61	1
NM_015225	KIAA0367	1
NM_015641	TES	1
NM_152829	TES	1
XM_001131606	LOC731003	1
XM_001132798	LOC729040	1
NM_173598	KSR2	1
NM_001626	AKT2	1
NM_152900	MAGI3	1
NM_130847	AMOTL1	1
NM_014661	FAM53B	1
NM_001337	CX3CR1	1
NM_020336	KIAA1219	1
NM_001025232	CLLU1OS	1
NM_018018	SLC38A4	1
XM_001125933	LOC727737	1
NM_030645	SH3BP5L	1
XM_001130495	LOC728429	1
NM_002695	POLR2E	1
NM_145206	VTI1A	1
XM_039733	KIAA0953	1
XM_940464	KIAA0953	1
NM_002375	MAP4	1
NM_030884	MAP4	1
NM_020448	NPAL3	1
NM_015399	BRMS1	1
NM_003734	AOC3	1
NM_003087	SNCG	1
NM_002716	PPP2R1B	1
NM_080621	SAMD10	1
NM_138371	FAM113B	1
NM_133330	WHSC1	1
NM_001042424	WHSC1	1
NM_133331	WHSC1	1
NM_133335	WHSC1	1
NM_015296	DOCK9	1
NM_031899	GORASP1	1
NM_014562	OTX1	1
NM_015064	ERC1	1
NM_178039	ERC1	1
NM_178040	ERC1	1
NM_178038	ERC1	1
NM_178037	ERC1	1
NM_178457	C20orf174	1
NM_144997	FLCN	1
NM_007260	LYPLA2	1
NM_020251	ARRB1	1
NM_004041	ARRB1	1
NM_014212	HOXC11	1
NM_014732	KIAA0513	1
XM_927712	LOC440104	1
NM_023923	PHACTR4	1
NM_001048183	PHACTR4	1
NM_015039	NMNAT2	1
NM_170706	NMNAT2	1
NM_018439	IMPACT	1
NM_020705	TBC1D24	1
NM_175922	MGC35308	1
NM_004339	PTTG1IP	1
NM_173075	APBB2	1
NM_016376	ANKFY1	1
NM_032595	PPP1R9B	1
NM_006581	FUT9	1
NM_001933	DLST	1
NM_001012994		1
NM_173511	ALS2CR13	1
NM_153686	LCORL	1
NM_015177		1
NM_004472	FOXD1	1
NM_001420	ELAVL3	1
NM_032281	ELAVL3	1
NM_032124	HDHD2	1
NM_003924	PHOX2B	1
NM_001616	ACVR2A	1
NM_002025	AFF2	1
NM_032786	ZC3H10	1
NM_053052	C1orf142	1
NM_018697	LANCL2	1
NM_001102	ACTN1	1
NM_002855	PVRL1	1
NM_015332	NUDCD3	1
NM_030647	JHDM1D	1
NM_001013629	LOC340843	1
NM_001098844		1
NM_145663	DBF4B	1
NM_014046	MRPS18B	1
NM_145165	CHURC1	1
NM_001759	CCND2	1
NM_018660	ZNF395	1
NM_015272	KIAA1005	1
NM_001424	EMP2	1
XM_001129103	LOC729029	1
XM_001128912	LOC731238	1
NM_020692	GALNTL1	1
NM_004455	EXTL1	1
NM_003724	JRK	1
NM_000498	CYP11B2	1
NM_004992	MECP2	1
NM_001942	DSG1	1
NM_020792	AADACL1	1
XM_929931	LOC653489	1
NM_005054	RGPD5	1
XM_001126221	LOC727851	1
XM_001126260	LOC727851	1
XM_001133591	LOC729540	1
XM_001133590	LOC729540	1
XM_001126233	LOC727851	1
XM_001126248	LOC727851	1
XM_001126272	LOC727851	1
NM_015831	ACHE	1
NM_002570	PCSK6	1
NM_138319	PCSK6	1
NM_006593	TBR1	1
NM_033118	MYLK2	1
XM_001127615	LOC730882	1
XM_001132036	LOC730002	1
XM_001126460	LOC730593	1
XM_001132387	LOC730094	1
NM_003557	PIP5K1A	1
NM_016518	PIPOX	1
NM_020382	SETD8	1
NM_000432	MYL2	1
NM_144579	SFXN5	1
NM_015635	GAPVD1	1
NM_004491	GRLF1	1
NM_015514	NGDN	1
NM_001097620		1
NM_017450	BAIAP2	1
NM_016337	EVL	1
NM_032199	ARID5B	1

---

Immunostimulatory motif search results

UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).

GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).

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Settings used for search

Scores were calculated using scoring scheme SS1
Using AVG scoring mode.

Hit scores

Parameter	Spec. Score cut-off	Colour	Comment
Hit to same gene		Colour	Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit.
High scoring hit	≥80	Colour	Hits are very likely to cause an off-target effect
Medium scoring hit	<80¸≥40	Colour	Hits with a small chance of causing an off target effect.
Low scoring hit		Colour	Hits very unlikely to cause an off-target effect

The mismatches are colour coded based on the weighting of that position.

Cut-off	Colour
Match	Colour
Wobble base pairs (G-U)	Colour
Critical bases (none)	Colour
0.6 bits	Colour
0.4 bits	Colour
0.2 bits	Colour

About SpecificityServer

SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).

Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer

For more details about the algorithm "siRNA specificity searching incorporating mismatch tolerance data" Bioinformatics (2008) 24 (10): 1316-1317