VIRsiRNAdb

SpecificityServer - An siRNA design program for determining siRNA specificity
Automatic recommendation
OK - siRNA is specific for both sense and antisense (guide) strands

30 hits were found for GGGCAAUGGUUGUGGGCUA (19 guide strand hits and 11 sense strand hits.)

Hits to Guide strand

Accession

Gene

Start

End

Details of mismatches

Spec. Score

Mismatches

Contiguous

Jackson style specificity

Hits similar gene

HSP Score

HSP % Identity

HSP length

Gene Length

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

NM_013320

HCFC2

1977

1995

a

a

u

C

A

A

U

G

G

U

U

G

U

G

G

G

a

U

A

43

4

13

c

false

<90%

2583

NM_198242

EIF4G1

38

56

c

G

G

a

A

A

U

G

G

U

U

G

U

G

G

a

C

U

A

35

3

11

c

false

<90%

5048

NM_198244

EIF4G1

38

56

c

G

G

a

A

A

U

G

G

U

U

G

U

G

G

a

C

U

A

35

3

11

c

false

<90%

5225

NM_198241

EIF4G1

38

56

c

G

G

a

A

A

U

G

G

U

U

G

U

G

G

a

C

U

A

35

3

11

c

false

<90%

5463

XM_001130226

LOC729425

252

270

a

G

G

C

A

A

U

G

G

a

U

G

U

G

G

G

g

U

A

-8

3

8

c

false

<90%

699

NM_174930

PMS2L5

249

267

a

G

G

C

A

A

U

G

G

a

U

G

U

G

G

G

g

U

A

-8

3

8

c

false

<90%

748

XM_001128073

LOC730313

252

270

a

G

G

C

A

A

U

G

G

a

U

G

U

G

G

G

g

U

A

-8

3

8

c

false

<90%

768

XM_001128562

LOC730324

252

270

a

G

G

C

A

A

U

G

G

a

U

G

U

G

G

G

g

U

A

-8

3

8

c

false

<90%

768

XM_001129884

LOC729299

252

270

a

G

G

C

A

A

U

G

G

a

U

G

U

G

G

G

g

U

A

-8

3

8

c

false

<90%

768

XM_001130276

LOC441259

252

270

a

G

G

C

A

A

U

G

G

a

U

G

U

G

G

G

g

U

A

-8

3

8

c

false

<90%

768

XM_001130281

LOC729453

252

270

a

G

G

C

A

A

U

G

G

a

U

G

U

G

G

G

g

U

A

-8

3

8

c

false

<90%

768

XM_001134451

PMS2L2

413

431

a

G

G

C

A

A

U

G

G

a

U

G

U

G

G

G

g

U

A

-8

3

8

c

false

<90%

1082

XM_001134437

PMS2L2

413

431

a

G

G

C

A

A

U

G

G

a

U

G

U

G

G

G

g

U

A

-8

3

8

c

false

<90%

1082

XM_001128484

LOC441259

252

270

a

G

G

C

A

A

U

G

G

a

U

G

U

G

G

G

g

U

A

-8

3

8

c

false

<90%

1214

XM_001128555

LOC730323

252

270

a

G

G

C

A

A

U

G

G

a

U

G

U

G

G

G

g

U

A

-8

3

8

c

false

<90%

1214

XM_001130610

PMS2L1

795

813

a

G

G

C

A

A

U

G

G

a

U

G

U

G

G

G

g

U

A

-8

3

8

c

false

<90%

1699

XM_001131056

PMS2L1

807

825

a

G

G

C

A

A

U

G

G

a

U

G

U

G

G

G

g

U

A

-8

3

8

c

false

<90%

1711

XM_001131976

LOC732139

828

846

a

G

G

C

A

A

U

G

G

a

U

G

U

G

G

G

g

U

A

-8

3

8

c

false

<90%

1732

NM_001014797

KCNMA1

10175

10193

G

G

G

C

A

A

U

G

G

c

U

G

U

G

G

G

u

c

u

-13

4

9

c

false

<90%

11993

Hits to Sense strand

Accession

Gene

Start

End

Details of mismatches

Spec. Score

Mismatches

Contiguous

Jackson style specificity

Hits similar gene

HSP Score

HSP % Identity

HSP length

Gene Length

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

NM_133337

FER1L3

6681

6699

a

u

G

C

C

C

A

C

A

A

C

C

A

U

U

G

C

u

a

78

4

15

ac

false

<90%

6790

NM_013451

FER1L3

6720

6738

a

u

G

C

C

C

A

C

A

A

C

C

A

U

U

G

C

u

a

78

4

15

ac

false

<90%

6829

NM_002565

P2RY4

469

487

c

A

G

C

C

C

A

C

A

A

C

C

A

c

U

G

g

C

C

54

3

12

c

false

<90%

1639

XM_001127139

LOC728307

522

540

g

A

G

C

C

C

A

C

A

g

C

C

A

U

U

G

C

C

C

15

2

9

bc

false

<90%

5805

XM_001127165

LOC728317

522

540

g

A

G

C

C

C

A

C

A

g

C

C

A

U

U

G

C

C

C

15

2

9

bc

false

<90%

6087

XM_001127959

LOC730979

522

540

g

A

G

C

C

C

A

C

A

g

C

C

A

U

U

G

C

C

C

15

2

9

bc

false

<90%

6186

NM_001042604

XRN1

3698

3716

g

A

G

C

C

C

u

C

A

A

C

C

A

U

U

c

C

C

C

5

3

8

c

false

<90%

10053

NM_019001

XRN1

3698

3716

g

A

G

C

C

C

u

C

A

A

C

C

A

U

U

c

C

C

C

5

3

8

c

false

<90%

10092

XM_936538

FAT3

10776

10794

a

A

G

C

C

C

A

C

A

g

C

C

A

U

U

c

C

C

C

-10

3

8

c

false

<90%

19162

XM_926199

FAT3

10776

10794

a

A

G

C

C

C

A

C

A

g

C

C

A

U

U

c

C

C

C

-10

3

8

c

false

<90%

19163

NR_002996

SNORA80

71

89

U

A

G

C

C

C

u

g

A

A

C

C

A

U

U

G

C

C

u

-33

3

10

bc

false

<90%

136

---

3 prime UTR Seed matches

Hits to same gene : 0
Hits to other genes : 1022
View matched accessions

3 prime seed match details

Accession	Gene name	Hits to this gene
NM_001080412	ZBTB38	3
XM_001133510	ZBTB38	3
NM_003679	KMO	2
NM_018263	ASXL2	2
NM_021960	MCL1	2
NM_182763	MCL1	2
XM_939547	ZNF697	2
NM_001080470	ZNF697	2
NM_182969		2
NM_024345	WDR32	2
NM_022153	C10orf54	2
NM_182612	PDDC1	2
XM_001128311	LOC731449	2
XM_001129750	LOC728999	2
NM_001099678		2
NM_002211	ITGB1	2
NM_001037339	PDE4B	2
NM_002600	PDE4B	2
NM_001037340	PDE4B	2
NM_001037341	PDE4B	2
NM_020127	TUFT1	2
NM_014112	TRPS1	2
NM_005957	MTHFR	2
NM_000633	BCL2	2
NM_006566	CD226	2
NM_000831	GRIK3	2
XM_942692	MGC5139	2
NM_001080533	MGC5139	2
NM_025057	C14orf45	2
NM_006722	MITF	2
NM_000248	MITF	2
NM_198158	MITF	2
NM_198177	MITF	2
NM_198178	MITF	2
NM_198159	MITF	2
NM_001081573	GAB3	2
NM_080612	GAB3	2
NM_173462	PAPLN	2
NM_025179	PLXNA2	2
NM_001113239		2
NM_022740	HIPK2	2
NM_201628	FLJ43806	2
NM_001040285	PAPD5	2
NM_001040284	PAPD5	2
NM_024577	SH3TC2	2
NM_000429	MAT1A	2
NM_024569	MPZL1	2
NM_003953	MPZL1	2
NM_032738	FCRLA	2
NM_004050	BCL2L2	2
NM_004331	BNIP3L	1
NM_001082480	ZNF623	1
NM_014789	ZNF623	1
NM_001077440	BCLAF1	1
NM_001077441	BCLAF1	1
NM_014739	BCLAF1	1
NM_004869	VPS4B	1
NM_000256	MYBPC3	1
NM_152380	TBX15	1
NM_005745	BCAP31	1
XM_173119	LOC255130	1
XM_943077	LOC647875	1
NM_152703	SAMD9L	1
NM_004992	MECP2	1
NM_033439	IL33	1
NM_025216	WNT10A	1
NM_153265	EML3	1
NM_004556	NFKBIE	1
NM_000929	PLA2G5	1
NM_173588	IGSF22	1
NM_145259	ACVR1C	1
NM_001111031		1
NM_001111033		1
NM_001111032		1
NM_015999	ADIPOR1	1
NM_138420		1
NM_198560	LHFPL4	1
NM_207363	NAP5	1
NM_207481	NAP5	1
NM_152446	C14orf145	1
NM_003755	EIF3S4	1
NM_182686	KIAA0319L	1
NM_022830	TUT1	1
NM_000290	PGAM2	1
NM_182802	MCM8	1
NM_032485	MCM8	1
NM_174878	CLRN1	1
NM_144579	SFXN5	1
NM_014021	SSX2IP	1
NM_015025	MYT1L	1
NM_000877	IL1R1	1
NM_000713	BLVRB	1
NM_020336	KIAA1219	1
NM_033427	CTTNBP2	1
NM_006581	FUT9	1
NM_000163	GHR	1
NM_001040446	MTMR12	1
NM_194298	SLC16A9	1
XM_001132813	LOC728741	1
XM_001132780	LOC728734	1
NM_003292	TPR	1
NM_015035	ZHX3	1
NM_019119	PCDHB9	1
XM_001132516	LOC729049	1
NM_177538	CYP20A1	1
NM_024068	OBFC2B	1
NM_021645	UTP14C	1
NM_194247	HNRPA3	1
NM_012069	ATP1B4	1
NM_000693	ALDH1A3	1
NM_005741	ZNF263	1
NM_001336	CTSZ	1
NM_003305	TRPC3	1
NM_017778	WHSC1L1	1
NM_032445	MEGF11	1
NM_130794	CST11	1
NM_080830	CST11	1
NM_206909	PSD3	1
NM_015310	PSD3	1
NM_015514	NGDN	1
NM_152437	ZNF664	1
NM_001270	CHD1	1
NM_022459	XPO4	1
XM_379623	FLJ41200	1
XM_943473	FLJ41200	1
NM_017774	CDKAL1	1
NM_013269	CLEC2D	1
NM_001004419	CLEC2D	1
NM_004588	SCN2B	1
NM_020828	ZFP28	1
NM_020921	NIN	1
XM_946032	LOC644852	1
XM_934218	LOC644852	1
NM_005540	INPP5B	1
NM_003442	ZNF143	1
XM_932982	LOC645586	1
NM_003594	TTF2	1
NM_001011516	PDLIM5	1
NM_001011515	PDLIM5	1
NM_018105	THAP1	1
NM_199003	THAP1	1
NM_006646	WASF3	1
NM_152498	WDR65	1
NM_005506	SCARB2	1
NM_012318	LETM1	1
NM_020781	ZNF398	1
NM_170686	ZNF398	1
NM_078476	BTN2A1	1
NM_005381	NCL	1
NM_032453		1
NM_024052	C17orf39	1
NM_022893	BCL11A	1
NM_014663	JMJD2A	1
NM_005658	TRAF1	1
NM_130446	KLHL6	1
NM_001003793	RBMS3	1
NM_001003792	RBMS3	1
NM_144979	MGC27016	1
NM_018332	DDX19A	1
NM_001010867	C1orf69	1
NM_005603	ATP8B1	1
NM_153260	LRRC57	1
NM_021821	MRPS35	1
NM_198465	NRK	1
NM_032765	TRIM52	1
NM_007175	ERLIN2	1
NM_175924	ILDR1	1
NM_001879	MASP1	1
NM_024653	PRKRIP1	1
NM_001040450	FAM63B	1
NM_001040453	FAM63B	1
NM_002061	GCLM	1
NM_199050	C21orf25	1
NM_015500	C21orf25	1
XM_032945	C21orf25	1
NM_001024843	TNRC6B	1
NM_015088	TNRC6B	1
NM_024807	TREML2	1
NM_152280	SYT11	1
XM_944290	KIAA0492	1
XM_378914	KIAA0492	1
NM_033220	TRIM14	1
NM_145268	C7orf45	1
NM_001099666		1
NM_001259	CDK6	1
NM_018003	UACA	1
NM_001008224	UACA	1
NM_175575	WFIKKN2	1
NM_015138	RTF1	1
NM_006974	ZNF33A	1
NM_006954	ZNF33A	1
NM_001040402	DCUN1D4	1
NM_015115	DCUN1D4	1
NM_016388	TRAT1	1
NM_031453	FAM107B	1
NM_006805	HNRPA0	1
NM_006139	CD28	1
NM_021083	XK	1
NM_024843	CYBRD1	1
NM_014659	HISPPD2A	1
NM_001024463	HISPPD2A	1
NM_024596	MCPH1	1
NM_015187	KIAA0746	1
NM_001003954	ANXA13	1
NM_004306	ANXA13	1
NM_004200	SYT7	1
NM_012306	FAIM2	1
XM_945019	LOC643441	1
XM_933124	LOC643441	1
XM_001133002	LOC728804	1
NM_020776	KIAA1328	1
NM_177531	PKHD1L1	1
NM_018176	LGI2	1
NM_004984	KIF5A	1
NM_032193	RNASEH2C	1
NM_001032363	C1orf151	1
NM_052818	CG018	1
NM_001079691	CG018	1
NM_005347	HSPA5	1
NM_178342	C3orf35	1
NM_001025079	CD47	1
NM_001777	CD47	1
NM_198793	CD47	1
NM_002023	FMOD	1
NM_133478	SLC4A5	1
NM_001627	ALCAM	1
NM_005602	CLDN11	1
NM_001030288	SPN	1
NM_003123	SPN	1
NM_015458	MTMR9	1
XM_943832	MIA3	1
XM_932515	MIA3	1
XM_934649	C1orf34	1
XM_945958	C1orf34	1
NM_001017371	SP3	1
NM_003111	SP3	1
NM_000809	GABRA4	1
NM_080723	NRSN1	1
NM_145200	CABP4	1
NM_021646	ZNF500	1
NM_014820	TOMM70A	1
NM_016641	MIR16	1
NM_004843	IL27RA	1
NM_198968	DZIP1	1
NM_014934	DZIP1	1
NM_003879	CFLAR	1
NM_198849	LOC283514	1
XM_001132907	LOC730202	1
XM_001131136	LOC731887	1
NM_018173	PLEKHG6	1
NM_001012729	DUXA	1
NM_078628	MSL3L1	1
NM_001040441	ZBTB8	1
XM_001124597	LOC730411	1
NM_052928	SMYD4	1
NM_006212	PFKFB2	1
NM_031448	C19orf12	1
NM_001031726	C19orf12	1
NM_017831	RNF125	1
NM_146388	MRPL4	1
NM_052937	PCMTD1	1
NM_016315	GULP1	1
NM_013281	FLRT3	1
NM_198391	FLRT3	1
NM_020466	LYRM2	1
NM_001042357	PTPN20B	1
NM_001042358	PTPN20B	1
NM_001042365	PTPN20B	1
NM_001042361	PTPN20B	1
NM_001042364	PTPN20B	1
NM_001042360	PTPN20B	1
NM_001042363	PTPN20B	1
NM_015605	PTPN20B	1
NM_001042362	PTPN20B	1
NM_001042359	PTPN20B	1
NM_001042393	PTPN20A	1
NM_001042389	PTPN20A	1
NM_001042392	PTPN20A	1
NM_001042394	PTPN20A	1
NM_001042396	PTPN20A	1
NM_001042397	PTPN20A	1
NM_001042395	PTPN20A	1
NM_001042387	PTPN20A	1
NM_001042390	PTPN20A	1
NM_001042391	PTPN20A	1
NM_021633	KLHL12	1
NM_018941	CLN8	1
NM_006085	BPNT1	1
NM_006534	NCOA3	1
NM_181659	NCOA3	1
NM_031469	SH3BGRL2	1
NM_005230	ELK3	1
NM_000239	LYZ	1
NM_001905	CTPS	1
NM_012297	G3BP2	1
NM_203504	G3BP2	1
NM_203505	G3BP2	1
NM_014028	OSTM1	1
NM_015659	RSL1D1	1
NM_015174	KIAA1086	1
NM_173666	DTWD2	1
NM_001042574	CRTC3	1
NM_022769	CRTC3	1
NM_001656	TRIM23	1
NM_176815	DHFRL1	1
NM_001111283		1
NM_000618	IGF1	1
NM_001111284		1
NM_001083946		1
NM_144736	PRO1853	1
NM_018712	ELMOD1	1
NM_003468	FZD5	1
NM_002024	FMR1	1
NM_005164	ABCD2	1
NM_138786	TM4SF18	1
NM_012400	PLA2G2D	1
NM_018440	PAG1	1
NM_007350	PHLDA1	1
NM_005184	CALM3	1
NM_015000	STK38L	1
NM_178517	PIGW	1
NM_006266	RALGDS	1
NM_001042368	RALGDS	1
NM_018194	HHAT	1
NM_018057	SLC6A15	1
NM_001101396		1
NM_001101386		1
NM_006628	ARPP-19	1
NM_001003698	RREB1	1
NM_001003699	RREB1	1
NM_002955	RREB1	1
NM_178498	SLC5A12	1
NM_020718	USP31	1
NM_005845	ABCC4	1
NM_003968	UBE1C	1
NM_198195	UBE1C	1
NM_172060	EYA1	1
NM_000503	EYA1	1
NM_172058	EYA1	1
NM_172059	EYA1	1
NM_006587	CORIN	1
NM_173826	C3orf23	1
NM_002959	SORT1	1
NM_016441	CRIM1	1
NM_001040445	ASB1	1
NM_015852	ZNF117	1
NM_004593	SFRS10	1
NM_015901	NUDT13	1
NM_003421	ZNF37A	1
NM_001007094	ZNF37A	1
NM_181654	CPLX4	1
NM_014465	SULT1B1	1
NM_181534	KRT25	1
NM_181054	HIF1A	1
NM_001082971	DDC	1
NM_000790	DDC	1
NM_001468	GAGE1	1
XM_001129295	LOC728731	1
NM_007030	TPPP	1
NM_032977	CASP10	1
NM_001230	CASP10	1
NM_000242	MBL2	1
NM_020125	SLAMF8	1
NM_020803	KLHL8	1
NM_080591	PTGS1	1
NM_000962	PTGS1	1
NM_015297		1
NM_014709	USP34	1
NM_021949	ATP2B3	1
NM_001001344	ATP2B3	1
NM_032287	LDOC1L	1
NM_001099225		1
NM_144609	CCDC43	1
NM_177972	TUB	1
NM_003320	TUB	1
NM_173499	SPATA8	1
NM_012192	FXC1	1
NM_152680	TMEM154	1
NM_012082	ZFPM2	1
NM_005282	GPR4	1
NM_001002254	DGAT2L4	1
NM_001002295	GATA3	1
NM_002051	GATA3	1
NM_001005473	PLCXD3	1
NM_000512	GALNS	1
NM_001005404	YPEL2	1
NM_004171	SLC1A2	1
NM_005465	AKT3	1
NM_001112800		1
NM_021097	SLC8A1	1
NM_001112801		1
NM_001112802		1
XM_930750	LOC642366	1
XM_943652	LOC642366	1
NM_032817	ITIH5	1
NM_030569	ITIH5	1
NM_014944	CLSTN1	1
NM_001009566	CLSTN1	1
NM_000348	SRD5A2	1
NM_022077	MANBAL	1
NM_001003897	MANBAL	1
XM_934118	LOC653489	1
XM_934115	LOC653489	1
NM_007214	SEC63	1
NM_000418	IL4R	1
NM_001079846	CREBBP	1
NM_004380	CREBBP	1
NM_022170	EIF4H	1
NM_031992	EIF4H	1
NM_021794	ADAM30	1
NM_017547	FOXRED1	1
NM_203327	SLC23A2	1
NM_005116	SLC23A2	1
NM_133443	GPT2	1
XM_929433	LOC644006	1
XM_939111	LOC650020	1
NM_005507	CFL1	1
NM_178502	DTX3	1
NM_004283	RAB3D	1
NM_016442	ARTS-1	1
NM_002537	OAZ2	1
NM_004788	UBE4A	1
NM_014731	ProSAPiP1	1
NM_153685	C12orf53	1
NM_004494	HDGF	1
NM_016408	CDK5RAP1	1
NM_016082	CDK5RAP1	1
NM_020746	VISA	1
NM_001001734	ATP1A4	1
NM_144699	ATP1A4	1
NM_016135	ETV7	1
NM_173602	DIP2B	1
NM_005638	SYBL1	1
NM_001017395	TMCC1	1
NM_015008	TMCC1	1
XM_380092	LOC285941	1
XM_379477	LOC285941	1
XM_938991	LOC285941	1
NM_033429	CALML4	1
NM_001031733	CALML4	1
XM_378973	LOC284801	1
XM_944897	LOC284801	1
NM_002444	MSN	1
XM_943541	LOC344595	1
XM_001128525	LOC344595	1
NM_182767	SLC6A15	1
NM_004774	PPARBP	1
NM_173463	DKFZp761B107	1
NM_015207		1
NM_152637	METTL7B	1
NM_003483	HMGA2	1
NM_147202	C9orf25	1
NM_182848	CLDN10	1
NM_006984	CLDN10	1
NM_145071	CISH	1
XM_001134321	LOC732206	1
NM_004289	NFE2L3	1
NM_019886	CHST7	1
XM_496115	LOC440338	1
XM_940659	LOC440338	1
XM_001127385	LOC730776	1
XM_001128769	LOC728825	1
NM_006937	SUMO2	1
NM_001005849	SUMO2	1
NM_001944	DSG3	1
NM_173851	SLC30A8	1
NM_153029	N4BP1	1
NM_152613	WBP2NL	1
NM_000128	F11	1
NM_017516	RAB39	1
NM_021982	SEC24A	1
NM_003957	BRSK2	1
NM_000298	PKLR	1
NM_181871	PKLR	1
NM_016221	DCTN4	1
NM_000075	CDK4	1
NM_015520	MAGI1	1
NM_001033057	MAGI1	1
NM_017994	C7orf42	1
NM_145665	SPANXE	1
NM_022661	SPANXC	1
NM_145662	SPANXA2	1
NM_013453	SPANXA1	1
NM_000189	HK2	1
NM_019045	WDR44	1
NM_002251	KCNS1	1
NM_002499	NEO1	1
NM_000337	SGCD	1
NM_017709	FAM46C	1
NM_175066	DDX51	1
NM_004296	RGS6	1
NM_016472	C14orf129	1
XM_943323	LOC646719	1
XM_933842	LOC646719	1
NM_144578	C14orf32	1
NM_017923	MARCH1	1
XM_001130495	LOC728429	1
NM_001039615	LOC440053	1
XM_001130086	LOC728957	1
NM_001004328	ZNF705A	1
NM_012199	EIF2C1	1
NM_032417	SPANXD	1
NM_012173	FBXO25	1
NM_183420	FBXO25	1
NM_183421	FBXO25	1
NM_013997	TAC1	1
NM_013998	TAC1	1
NM_003182	TAC1	1
NM_013996	TAC1	1
NM_002193	INHBB	1
NM_000212	ITGB3	1
NM_017525	CDC42BPG	1
NM_024639	ZNF322A	1
NM_003393	WNT8B	1
NM_018178	GOLPH3L	1
NM_032943	SYTL2	1
NM_206929	SYTL2	1
NM_206930	SYTL2	1
NM_206927	SYTL2	1
NM_032379	SYTL2	1
NM_206928	SYTL2	1
NM_178173	CCDC36	1
NM_194071	CREB3L2	1
NM_007173	PRSS23	1
NM_144670	A2ML1	1
NM_005562	LAMC2	1
NM_002501	NFIX	1
NM_178568	RTN4RL1	1
NM_003045	SLC7A1	1
NM_006650	CPLX2	1
NM_001008220	CPLX2	1
NM_198334	GANAB	1
NM_198335	GANAB	1
NM_001040665	STEAP2	1
NM_152999	STEAP2	1
NM_001761	CCNF	1
NM_198276	TMEM17	1
NM_013230	CD24	1
NM_003110	SP2	1
NM_012164	FBXW2	1
NM_001013641	LOC388595	1
NM_000361	THBD	1
NM_181354	OXR1	1
NM_032105	PPP1R12B	1
NM_002481	PPP1R12B	1
NM_015092	SMG1	1
NM_016575	NT5DC3	1
NM_020708	SLC12A5	1
NM_016224	SNX9	1
NM_024325	ZNF343	1
NM_003146	SSRP1	1
XM_933539	MGC3032	1
XM_944529	MGC3032	1
NM_000498	CYP11B2	1
NM_014945	ABLIM3	1
NM_015053	PPFIA4	1
NM_019063	EML4	1
NM_153320	SLC22A7	1
NM_006672	SLC22A7	1
NM_002581	PAPPA	1
NM_017563	IL17RD	1
NM_001080973	IL17RD	1
XM_498841	LOC440748	1
NM_173681	ATG9B	1
NM_004451	ESRRA	1
XM_001133925	GRIP1	1
XM_001133901	GRIP1	1
XM_001133924	GRIP1	1
XM_001133902	GRIP1	1
XM_933903	LOC644662	1
XM_944785	LOC644662	1
NM_001481	GAS8	1
NM_014437	SLC39A1	1
NM_033437	PDE5A	1
NM_001083	PDE5A	1
NM_033430	PDE5A	1
NM_181727	SPATA12	1
NM_005065	SEL1L	1
NM_052924	RHPN1	1
NM_144599	NIPA1	1
NM_014448	ARHGEF16	1
NM_198571	FLJ39237	1
NM_139278	LGI3	1
XM_931945	LOC643962	1
XM_943665	LOC643962	1
NM_002198	IRF1	1
XM_379273	FLJ14186	1
XM_001130364	LOC728417	1
XM_001130184	LOC730843	1
NM_138554	TLR4	1
NM_003413	ZIC3	1
NM_080836	STK35	1
NM_030805	LMAN2L	1
NM_017888	FLJ20581	1
NM_006705	GADD45G	1
NM_012331	MSRA	1
NM_178006	STARD13	1
NM_178007	STARD13	1
NM_052851	STARD13	1
NM_138775	ALKBH8	1
NM_022648	TNS1	1
NM_003462	DNALI1	1
NM_014466	TEKT2	1
NM_021012	KCNJ12	1
NM_017750	RETSAT	1
NM_173578	C11orf72	1
XM_942240	LOC650557	1
NM_007153	ZNF208	1
NM_000494	COL17A1	1
NM_018234	STEAP3	1
NM_001008410	STEAP3	1
NM_182915	STEAP3	1
NM_018363	C10orf59	1
NM_001160	APAF1	1
NM_181868	APAF1	1
NM_181869	APAF1	1
NM_013229	APAF1	1
NM_181861	APAF1	1
NM_005334	HCFC1	1
NM_177963	SYT12	1
NM_032338	C12orf31	1
NM_021167	GATAD1	1
NM_133509	RAD51L1	1
NM_031435	THAP2	1
NM_015074	KIF1B	1
NM_004719	SFRS2IP	1
NM_014423	AFF4	1
NM_152284	CHMP4C	1
NM_001098819		1
NM_001098818		1
NM_000923	PDE4C	1
NM_005431	XRCC2	1
NM_006380	APPBP2	1
NM_033542	SYS1	1
NM_020132	AGPAT3	1
NM_001037553	AGPAT3	1
NM_016143	NSFL1C	1
NM_182483	NSFL1C	1
NM_018839	NSFL1C	1
NM_145733	SEPT3	1
NM_019106	SEPT3	1
NM_001003760	KLHL31	1
NM_003995	NPR2	1
NM_001001694	FLJ41993	1
NM_006315	PCGF3	1
NM_001039380	C10orf25	1
NM_198098	AQP1	1
NM_002988	CCL18	1
NM_001025092	MBP	1
NM_002385	MBP	1
NM_001025081	MBP	1
NM_001025090	MBP	1
NM_001025101	MBP	1
NM_145698	ACBD5	1
NM_001042473	ACBD5	1
NM_016083	CNR1	1
NM_033181	CNR1	1
NM_025078	PQLC1	1
NM_021962	ABR	1
NM_001092	ABR	1
NM_012208	HARS2	1
NM_001014797	KCNMA1	1
NM_001033855	DCLRE1C	1
NM_001033857	DCLRE1C	1
NM_001033858	DCLRE1C	1
NM_022487	DCLRE1C	1
NM_001410	MEGF8	1
NM_001460	FMO2	1
NM_000657	BCL2	1
NM_022304	HRH2	1
NM_002866	RAB3A	1
NM_198584	CA13	1
NM_080669	SLC46A1	1
NM_024756	MMRN2	1
NM_004722	AP4M1	1
NM_005456	MAPK8IP1	1
NM_020857	VPS18	1
NM_032588	TRIM63	1
NM_144675	GSG1L	1
NM_001109763		1
NM_000750	CHRNB4	1
NM_145912	NFAM1	1
NM_153333	TCEAL8	1
NM_001006684	TCEAL8	1
NM_012409	PRND	1
NM_005941	MMP16	1
NM_002247	KCNMA1	1
NM_000292	PHKA2	1
NM_005028	PIP5K2A	1
NM_001099272		1
NM_152733	BTBD9	1
NM_052893		1
NM_013245	VPS4A	1
NM_004571	PKNOX1	1
NM_003831	RIOK3	1
NM_198490	RAB43	1
NM_015381	FAM19A5	1
NM_001082967	FAM19A5	1
NM_020338	ZMIZ1	1
NM_006667	PGRMC1	1
NM_000157	GBA	1
NM_001005742	GBA	1
NM_001005749	GBA	1
NM_001005750	GBA	1
NM_001005741	GBA	1
NM_001918	DBT	1
NM_145810	CDCA7	1
NM_031942	CDCA7	1
NM_018131	CEP55	1
NM_020802	KIAA1377	1
NM_032391	PRAC	1
NM_001007024	GOSR1	1
NM_001007025	GOSR1	1
NM_004871	GOSR1	1
NM_018235	CNDP2	1
NM_052832	SLC26A7	1
NM_178820	FBXO27	1
NM_207411	XKR5	1
NM_018228	C14orf115	1
NM_152608	C1orf55	1
NM_000130	F5	1
NM_138923	TAF1	1
NM_004606	TAF1	1
NM_032339	C17orf37	1
NM_052906	LRRC62	1
NM_001080395	AATK	1
NM_198461	LONRF2	1
NM_020657	ZNF304	1
NM_033274	ADAM19	1
NM_013381	TRHDE	1
NM_145230	ATP6V0E2	1
NM_001100592		1
NM_181359	IL6R	1
NM_000565	IL6R	1
NM_002095	GTF2E2	1
NM_003933	BAIAP3	1
NM_001037165	FOXK1	1
NM_000318	PXMP3	1
NM_001079867	PXMP3	1
NM_144974	CCDC122	1
NM_018085	IPO9	1
NM_001024380	TM2D2	1
NM_078473	TM2D2	1
NM_001024381	TM2D2	1
NM_031940	TM2D2	1
NM_000297	PKD2	1
NM_019079	L1TD1	1
NM_001098833		1
NM_020218		1
NM_145025	C6orf199	1
NM_025218	ULBP1	1
NM_152493	FLJ25476	1
NM_020327	ACVR1B	1
NM_017728	TMEM104	1
NM_176823	S100A7A	1
NM_001002860	BTBD7	1
NM_020709		1
NM_194282	LIN54	1
NM_178818	CMTM4	1
NM_181521	CMTM4	1
NM_173728	ARHGEF15	1
NM_002986	CCL11	1
NM_001042633	SNX21	1
NM_033421	SNX21	1
NM_001042632	SNX21	1
NM_017548	CDV3	1
NM_002709	PPP1CB	1
NM_206876	PPP1CB	1
NM_182557	BCL9L	1
NM_006247	PPP5C	1
NM_001001669	FLJ41603	1
NM_017860	C1orf56	1
NM_017665	ZCCHC10	1
NM_054111	IHPK3	1
NM_000782	CYP24A1	1
NM_138395	MARS2	1
NM_012166	FBXO10	1
NM_005595	NFIA	1
NM_152722	HEPACAM	1
NM_014805	EPM2AIP1	1
NM_007106	UBL3	1
NM_006504	PTPRE	1
NM_130435	PTPRE	1
NM_006729	DIAPH2	1
NM_016488	PPHLN1	1
NM_001012339	DNAJA5	1
NM_194283	DNAJA5	1
NM_138468	ICA1L	1
NM_015313	ARHGEF12	1
NM_006682	FGL2	1
NM_007147	ZNF175	1
NM_018667	SMPD3	1
NM_004748	CCPG1	1
NM_020739	CCPG1	1
NM_024947	PHC3	1
NM_139319	SLC17A8	1
NM_001012418	LOC340156	1
NM_012074	DPF3	1
NM_001114	ADCY7	1
NM_022896	LPIN3	1
NM_173359	EIF4E3	1
NM_014395	DAPP1	1
NM_019116	UBFD1	1
XM_378876	LOC149351	1
XM_943142	LOC149351	1
NM_025027	ZNF606	1
NM_015351		1
NM_181845		1
NM_021201	MS4A7	1
NM_206938	MS4A7	1
NM_206940	MS4A7	1
NM_206939	MS4A7	1
NM_022080	NAPB	1
NM_199280	LOC165186	1
NM_001024647	RAB3IP	1
NM_022456	RAB3IP	1
NM_175624	RAB3IP	1
NM_175625	RAB3IP	1
NM_175623	RAB3IP	1
NM_000786	CYP51A1	1
NM_001527	HDAC2	1
NM_033224	PURB	1
NM_001029858	SLC35F1	1
NM_007114	TMF1	1
NM_016210	C3orf18	1
NM_004703	RABEP1	1
NM_001083585	RABEP1	1
NM_024638	QTRTD1	1
NM_016183	MRTO4	1
NM_004051	BDH1	1
NM_203315	BDH1	1
NM_203314	BDH1	1
NM_025074	FRAS1	1
NM_001101667		1
NM_003501	ACOX3	1
NM_018157	RIC8B	1
NM_015939	CGI-09	1
NM_012146	DUX1	1
NM_148903	GREB1	1
NM_005590	MRE11A	1
NM_005591	MRE11A	1
NM_015289	VPS39	1
NM_001387	DPYSL3	1
NM_003482	MLL2	1
NM_014848	SV2B	1
NM_013382	POMT2	1
NM_133367	PAQR8	1
XM_379100	LOC400958	1
XM_945436	LOC400958	1
NM_003629	PIK3R3	1
NM_022898	BCL11B	1
NM_138576	BCL11B	1
NM_181876	PPP2R2C	1
NM_020416	PPP2R2C	1
NM_001001995	GPM6B	1
NM_014444	76P	1
NM_005937	MLLT6	1
NM_005440	RND2	1
NM_003043	SLC6A6	1
NM_024689	CXorf36	1
NM_002062	GLP1R	1
NM_007039	PTPN21	1
NM_018362	LIN7C	1
NM_080668	CDCA5	1
NM_001040167	LFNG	1
NM_003870	IQGAP1	1
NM_145263	SPATA18	1
NM_015078	MCF2L2	1
NM_001042697	ZSWIM7	1
NM_001042698	ZSWIM7	1
NM_018313	PBRM1	1
NM_181042	PBRM1	1
NM_018165	PBRM1	1
NM_002946	RPA2	1
NM_173582	PGM2L1	1
NM_006014	LAGE3	1
NM_018411	HR	1
NM_005144	HR	1
NM_015116	LRCH1	1
NM_006060	IKZF1	1
NM_014978	SORCS3	1
NM_021649	TICAM2	1
NM_015567	SLITRK5	1
XM_001127604	NFATC2IP	1
NM_032815	NFATC2IP	1
XM_942869	BOAT	1
XM_292512	BOAT	1
NM_007078	LDB3	1
NM_001080114	LDB3	1
NM_014744	TBC1D5	1
NM_001110213		1
NM_005042	PRH2	1
NM_006460	HEXIM1	1
NM_014231	VAMP1	1
NM_199245	VAMP1	1
NM_001099733		1
NM_001117	ADCYAP1	1
NM_002243	KCNJ15	1
NM_170736	KCNJ15	1
NM_170737	KCNJ15	1
XM_944080	LOC650193	1
XM_932038	LOC644192	1
NM_007203	PALM2-AKAP2	1
NM_147150	PALM2-AKAP2	1
NM_001004065	AKAP2	1
XM_932563	LOC643008	1
XM_942645	LOC643008	1
XM_928053	LOC643008	1
XM_946181	LOC643008	1
NM_001928	CFD	1
XM_940903	ZC3H5	1
NM_001080419	UNK	1
NM_178519	C17orf55	1
NM_021954	GJA3	1
NM_004381	CREBL1	1
NM_030895	ZNF696	1
NM_053280	ODF3	1
NM_019114	EPB41L4B	1
NM_175907	ZADH2	1
NM_183075	CYP2U1	1
NM_018373	SYNJ2BP	1
NM_173827	COX18	1
NM_004416	DTX1	1
NM_024016	HOXB8	1
NM_198269	HIPK1	1
NM_181358	HIPK1	1
NM_198268	HIPK1	1
NM_022465	IKZF4	1
NM_194452	RNF121	1
NM_194453	RNF121	1
NM_018320	RNF121	1
NM_014960	ARSG	1
NM_182526	C14orf83	1
NM_198511	LANCL3	1
NM_005985	SNAI1	1
NM_001454	FOXJ1	1
NM_138771	CCDC126	1
NM_014648	DZIP3	1
NM_004650	PNPLA4	1
NM_017658	KLHL28	1
NM_015657	ABCA12	1
NM_173076	ABCA12	1
XM_001132703	FLJ41047	1
XM_001133280	FLJ41047	1
NM_152998	EZH2	1
NM_004456	EZH2	1
NM_020811		1
NM_014059	C13orf15	1
NM_018302	C4orf19	1
NM_001104629		1
NM_012316	KPNA6	1
NM_020205	OTUD7B	1
NM_001039547	GK5	1
NM_002445	MSR1	1
XM_001126090	LOC728146	1
XM_001129476	LOC286434	1
XM_001129423	LOC729137	1
XM_928240	LOC389906	1
XM_942518	LOC389906	1
NM_031845	MAP2	1
NM_031847	MAP2	1
NM_001039538	MAP2	1
NM_002374	MAP2	1
NM_022725	FANCF	1
NM_014317	PDSS1	1
NM_022049	GPR88	1
NM_032358	CCDC77	1
NM_001010895	LOC375748	1
NM_021132	PPP3CB	1
XM_001130121	LOC729385	1
NM_017773	LAX1	1
NM_052918	SORCS1	1
NM_001013031	SORCS1	1
NM_015177		1
NM_001033081	MYCL1	1
NM_001033082	MYCL1	1
NM_080552	SLC32A1	1
XM_001125918	LOC727872	1
NM_012111	AHSA1	1
NM_198557	RBM43	1
NM_001039199	C20orf121	1
NM_024331	C20orf121	1
NM_002223	ITPR2	1
XM_939520	LOC441050	1
NM_024909	C6orf134	1
NM_020151	STARD7	1
NM_006328	RBM14	1
NM_025160	WDR26	1
NM_144633	KCNH8	1
NM_002451	MTAP	1
NM_015150	RFTN1	1
NM_001039580	MAP9	1
NM_007249	KLF12	1
NM_007342	NUPL2	1
NM_006601	PTGES3	1
NM_001097636		1
NM_001097634		1
NM_001097635		1
NM_001490	GCNT1	1
NM_001097633		1
NM_138969	RDHE2	1
NM_015185	ARHGEF9	1
NM_000262	NAGA	1
NM_001039479	KIAA0317	1
NM_021133	RNASEL	1
NM_199459	C10orf71	1
NM_174976	ZDHHC22	1
NM_020724	RNF150	1
NM_014522	PCDH11X	1
NM_007241	SNF8	1
NM_032446	MEGF10	1
NM_012130	CLDN14	1
NM_144492	CLDN14	1
NM_021130	PPIA	1
NM_017966	VPS37C	1
NM_001002814	RAB11FIP1	1
NM_001002233	RAB11FIP1	1
NM_025151	RAB11FIP1	1
NM_033637	BTRC	1
NM_003939	BTRC	1
NM_001031715	IQCH	1
NM_022784	IQCH	1

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Immunostimulatory motif search results

UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).

GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).

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Settings used for search

Scores were calculated using scoring scheme SS1
Using AVG scoring mode.

Hit scores

Parameter	Spec. Score cut-off	Colour	Comment
Hit to same gene		Colour	Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit.
High scoring hit	≥80	Colour	Hits are very likely to cause an off-target effect
Medium scoring hit	<80¸≥40	Colour	Hits with a small chance of causing an off target effect.
Low scoring hit		Colour	Hits very unlikely to cause an off-target effect

The mismatches are colour coded based on the weighting of that position.

Cut-off	Colour
Match	Colour
Wobble base pairs (G-U)	Colour
Critical bases (none)	Colour
0.6 bits	Colour
0.4 bits	Colour
0.2 bits	Colour

About SpecificityServer

SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).

Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer

For more details about the algorithm "siRNA specificity searching incorporating mismatch tolerance data" Bioinformatics (2008) 24 (10): 1316-1317