VIRsiRNAdb

SpecificityServer - An siRNA design program for determining siRNA specificity
Automatic recommendation
Warning - siRNA is specific for only the antisense (guide) strand

26 hits were found for CGGGAAAGACGAAGAGAUA (15 guide strand hits and 11 sense strand hits.)

Hits to Guide strand

Accession

Gene

Start

End

Details of mismatches

Spec. Score

Mismatches

Contiguous

Jackson style specificity

Hits similar gene

HSP Score

HSP % Identity

HSP length

Gene Length

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

NM_024621

VEPH1

608

626

u

G

G

G

A

A

A

G

A

C

G

A

A

G

A

c

A

c

u

64

4

14

c

false

<90%

3044

NM_183416

KIF1B

2699

2717

C

a

u

G

A

A

A

G

A

C

G

A

g

G

A

G

A

U

A

58

3

9

false

<90%

5780

XM_001132049

SEPT1

1166

1184

C

G

a

G

A

A

A

G

A

C

G

A

A

G

A

G

c

U

g

51

3

13

c

false

<90%

1569

NM_052838

SEPT1

1189

1207

C

G

a

G

A

A

A

G

A

C

G

A

A

G

A

G

c

U

g

51

3

13

c

false

<90%

1634

XM_942624

WDR42C

342

360

g

G

a

G

g

A

A

G

A

C

G

A

A

G

A

G

A

U

A

26

3

14

bc

false

<90%

1854

XM_293354

WDR42C

342

360

g

G

a

G

g

A

A

G

A

C

G

A

A

G

A

G

A

U

A

26

3

14

bc

false

<90%

1854

NM_006562

LBX1

958

976

C

G

a

G

g

A

A

G

A

C

G

A

A

G

A

G

A

U

c

23

3

13

bc

false

<90%

1287

NM_001012339

DNAJA5

923

941

g

a

G

G

A

A

A

G

c

C

G

A

A

G

A

G

A

U

g

21

4

9

bc

false

<90%

6083

NM_194283

DNAJA5

923

941

g

a

G

G

A

A

A

G

c

C

G

A

A

G

A

G

A

U

g

21

4

9

bc

false

<90%

6218

NM_024516

C16orf53

908

926

C

G

G

G

A

A

A

G

A

g

G

A

A

G

A

G

A

g

A

14

2

9

c

false

<90%

1164

NM_207482

FLJ44048

1001

1019

a

G

G

G

A

A

A

G

A

u

G

A

u

G

A

G

A

U

A

4

3

8

false

<90%

5088

NM_080604

TJAP1

154

172

u

u

G

G

A

A

A

G

A

a

G

A

A

G

A

G

A

U

A

3

3

9

bc

false

<90%

2720

NM_001008539

SLC7A2

3152

3170

a

G

G

G

A

A

A

G

A

u

G

A

A

G

g

G

A

U

A

-9

3

8

c

false

<90%

7434

NM_003046

SLC7A2

3295

3313

a

G

G

G

A

A

A

G

A

u

G

A

A

G

g

G

A

U

A

-9

3

8

c

false

<90%

7577

NM_020802

KIAA1377

1767

1785

u

G

G

u

A

A

A

G

A

a

G

A

A

G

A

G

A

U

A

-21

3

9

bc

false

<90%

7043

Hits to Sense strand

Accession

Gene

Start

End

Details of mismatches

Spec. Score

Mismatches

Contiguous

Jackson style specificity

Hits similar gene

HSP Score

HSP % Identity

HSP length

Gene Length

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

XM_001125986

LOC283824

8176

8194

U

A

U

C

U

C

U

U

C

G

U

C

c

U

U

C

C

u

G

86

2

12

false

<90%

9033

NM_022166

XYLT1

8483

8501

U

A

U

C

U

C

U

U

C

G

U

C

c

U

U

C

C

u

G

86

2

12

false

<90%

9336

NM_002654

PKM2

185

203

g

A

U

C

U

C

U

U

C

G

U

C

U

U

U

g

C

a

G

69

3

14

c

false

<90%

2479

NM_182471

PKM2

185

203

g

A

U

C

U

C

U

U

C

G

U

C

U

U

U

g

C

a

G

69

3

14

c

false

<90%

2498

NM_182470

PKM2

185

203

g

A

U

C

U

C

U

U

C

G

U

C

U

U

U

g

C

a

G

69

3

14

c

false

<90%

2674

NM_006125

ARHGAP6

3137

3155

c

u

U

C

U

C

U

U

C

G

U

C

U

c

U

C

C

C

u

62

4

11

c

false

<90%

3632

NM_013422

ARHGAP6

3233

3251

c

u

U

C

U

C

U

U

C

G

U

C

U

c

U

C

C

C

u

62

4

11

c

false

<90%

3728

NM_144697

C1orf51

491

509

U

c

U

C

U

C

U

U

C

G

U

C

U

U

U

u

C

C

c

57

3

13

c

false

<90%

1758

NM_012469

PRPF6

102

120

a

g

U

C

U

C

U

g

C

G

U

C

U

U

U

C

C

C

u

19

4

10

bc

false

<90%

3120

NM_144723

ZMAT2

924

942

U

A

U

C

U

C

U

U

C

a

U

C

U

a

U

C

C

C

a

-7

3

9

c

false

<90%

1512

NM_001406

EFNB3

1811

1829

U

A

U

C

U

C

U

U

a

u

U

C

U

U

U

C

C

C

u

-47

3

8

bc

false

<90%

3236

---

3 prime UTR Seed matches

Hits to same gene : 0
Hits to other genes : 569
View matched accessions

3 prime seed match details

Accession	Gene name	Hits to this gene
NM_004745	DLGAP2	3
XM_001125737	LOC730275	2
XM_936719	LOC647662	2
NM_130446	KLHL6	2
NM_133334	WHSC1	2
NM_015471	NSL1	2
NM_001042549	NSL1	2
NM_018346	RSAD1	2
NM_001097620		2
NM_020820	PREX1	2
XM_927997	LOC643338	2
XM_938980	LOC643338	2
NM_005297	MCHR1	2
NM_203343	EPB41	2
NM_203342	EPB41	2
NM_004437	EPB41	2
NM_012292	HMHA1	1
NM_000101	CYBA	1
NM_145805	ISL2	1
NM_145202	PRAP1	1
NM_020650	RCN3	1
NM_007372	DDX42	1
NM_203499	DDX42	1
NM_001005176	SP140	1
NM_014681	DHX34	1
NM_031229	RBCK1	1
NM_006462	RBCK1	1
NM_173164	NFATC3	1
NM_003019	SFTPD	1
NM_001099784		1
NM_001014283	DCUN1D2	1
NM_031482	ATG10	1
NM_002193	INHBB	1
NM_032510	PARD6G	1
XM_001129334	PARD6G	1
NM_001627	ALCAM	1
NM_152493	FLJ25476	1
NM_182925	FLT4	1
NM_020205	OTUD7B	1
NM_015896	ZMYND10	1
NM_004959	NR5A1	1
NM_017523	XAF1	1
NM_199139	XAF1	1
NM_001111283		1
NM_000618	IGF1	1
NM_001111284		1
NM_018650	MARK1	1
NM_024830	AYTL2	1
XM_001132925	DUSP22	1
NM_017728	TMEM104	1
NM_001079846	CREBBP	1
NM_004380	CREBBP	1
XM_379228	LOC401093	1
XM_944985	LOC401093	1
NM_024561	NARG1L	1
NM_207397	CD164L2	1
NM_006943	SOX12	1
NM_178586	PPP2R5C	1
NM_002719	PPP2R5C	1
NM_001098270		1
NM_002409	MGAT3	1
NM_006212	PFKFB2	1
NM_015093	MAP3K7IP2	1
NM_004808	NMT2	1
NM_005902	SMAD3	1
NM_145245	EVI5L	1
XM_001131577	FLJ36644	1
XM_001126095	FLJ36644	1
NM_001025233	CLLU1	1
NM_001039840	CHIC1	1
NM_198595	AFAP1	1
NM_021638	AFAP1	1
NM_153266	TMEM151	1
XM_001133285	TMEM151	1
NM_021946	BCORL1	1
NM_133487	RAD51	1
NM_002875	RAD51	1
NM_024785	FAM124B	1
XM_001134344	FLJ37453	1
NM_015172	BAT2D1	1
NM_032968	PCDH11X	1
NM_032969	PCDH11X	1
NM_001099294		1
XM_936510	KIAA1644	1
NM_000253	MTTP	1
XM_001132373	LOC730994	1
XM_001130568	LOC728392	1
XM_001130582	LOC728392	1
XM_001132379	LOC730994	1
NM_024963	FBXL18	1
NM_017970	C14orf102	1
NM_199043	C14orf102	1
XM_001131019	LOC731846	1
XM_001131201	LOC729751	1
NM_001042573	FLJ21865	1
NM_133468	BMPER	1
NM_052978	TRIM9	1
NM_016233	PADI3	1
NM_031418	TMEM16C	1
NM_004295	TRAF4	1
NM_020127	TUFT1	1
NM_020768	KCTD16	1
NM_153346	CXorf20	1
NM_001001331	ATP2B2	1
NM_001683	ATP2B2	1
NM_001040446	MTMR12	1
NM_019079	L1TD1	1
NM_005806	OLIG2	1
NM_014766	SCRN1	1
NM_181312	TAZ	1
NM_000116	TAZ	1
NM_181313	TAZ	1
NM_181311	TAZ	1
NM_198549	FAM73A	1
NM_001085382		1
NM_007084	SOX21	1
NM_033128	SCIN	1
NM_001112706		1
NM_015039	NMNAT2	1
NM_170706	NMNAT2	1
NM_002639	SERPINB5	1
NM_016087	WNT16	1
NM_057168	WNT16	1
NM_033011	PLAT	1
NM_000930	PLAT	1
NM_172101	CD8B	1
NM_172213	CD8B	1
NM_172102	CD8B	1
NM_024701	ASB13	1
NM_032595	PPP1R9B	1
NM_014333	CADM1	1
NM_001098517		1
NM_033081	DIDO1	1
XM_059954	C9orf57	1
XM_937019	C9orf57	1
NM_031911	C1QTNF7	1
NM_003074	SMARCC1	1
NM_001029939	ZFAT1	1
NM_033393	KIAA1727	1
NM_000135	FANCA	1
NM_001079863	DBI	1
NM_020548	DBI	1
NM_001079862	DBI	1
XM_001130020	KIAA1147	1
XM_001130081	KIAA1147	1
NM_001080392	KIAA1147	1
NM_144974	CCDC122	1
NM_001039577	CCNL2	1
NM_000208	INSR	1
NM_001079817	INSR	1
NM_000808	GABRA3	1
NM_005402	RALA	1
NM_001039130	ALOX15B	1
NM_001141	ALOX15B	1
NM_001039131	ALOX15B	1
NM_001080436	WTIP	1
XM_939855	WTIP	1
NM_174934	SCN4B	1
NM_177951	PPM1A	1
NM_007113		1
NM_022473	ZFP106	1
XM_932244	LOC653160	1
XM_945233	LOC653160	1
NM_003590	CUL3	1
NM_198076	FAM36A	1
NM_018439	IMPACT	1
NM_052867	NALCN	1
NM_033428	C9orf123	1
NM_013989	DIO2	1
NM_001007023	DIO2	1
NM_000793	DIO2	1
NM_018085	IPO9	1
NM_000990	RPL27A	1
NM_005318	H1F0	1
NM_020848		1
NM_171827	CD8A	1
NM_001768	CD8A	1
NM_006950	SYN1	1
NM_133499	SYN1	1
NM_003954	MAP3K14	1
XM_001133339	LOC731569	1
XM_001133506	LOC729534	1
NM_004539	NARS	1
NM_006529	GLRA3	1
NM_001042543	GLRA3	1
NM_014772	KIAA0427	1
NM_001010897	C13orf21	1
NM_015113	ZZEF1	1
NM_006838	METAP2	1
NM_032639	PLEKHA8	1
NM_003239	TGFB3	1
NM_001109810		1
NM_178498	SLC5A12	1
NM_015335	THRAP2	1
NM_001105579		1
NM_002664	PLEK	1
NM_024544	C1orf166	1
NM_024624	SMC6	1
NM_015847	MBD1	1
NM_015845	MBD1	1
NM_015844	MBD1	1
NM_002384	MBD1	1
NM_015846	MBD1	1
NM_032287	LDOC1L	1
NM_016083	CNR1	1
NM_033181	CNR1	1
NM_033254	BOC	1
NM_001009934	DNASE1L1	1
NM_001009932	DNASE1L1	1
NM_006730	DNASE1L1	1
NM_001009933	DNASE1L1	1
NM_033403		1
NM_020761	KIAA1303	1
NM_001116	ADCY9	1
NM_001415	EIF2S3	1
XM_001127052	LOC730568	1
XM_001130247	LOC728606	1
NM_012316	KPNA6	1
NM_001039570	KREMEN1	1
NM_001039571	KREMEN1	1
NM_016338	IPO11	1
NM_025153		1
NM_177925	H2AFJ	1
XM_001132480	SLCO3A1	1
NM_138348	FAM105B	1
NM_012315	KLK9	1
NM_032189	ATP11A	1
NM_015205	ATP11A	1
NM_017433	MYO3A	1
NM_022469	GREM2	1
XM_001126137	LOC284952	1
XM_001129340	LOC284952	1
NM_004778	GPR44	1
NM_000554	CRX	1
NM_002500	NEUROD1	1
NM_016173	HEMK1	1
NM_004756	NUMBL	1
NM_144688	FLJ32658	1
NM_173551	ANKS6	1
XM_943278	LOC644727	1
XM_932334	LOC644727	1
NM_015695	BRPF3	1
NM_015493	ANKRD25	1
NM_005766	FARP1	1
NM_001013662		1
NM_004824	CDYL	1
NM_170751	CDYL	1
NM_170752	CDYL	1
NM_001105	ACVR1	1
NM_004416	DTX1	1
NM_006549	CAMKK2	1
NM_153499	CAMKK2	1
NM_153500	CAMKK2	1
NM_172216	CAMKK2	1
NM_172226	CAMKK2	1
NM_183040	DTNBP1	1
NM_001031672	LOC606495	1
NM_005813	PRKD3	1
NM_001043318	C14orf43	1
NM_194278	C14orf43	1
NM_001003406	CACNA1I	1
NM_021096	CACNA1I	1
NM_012099	CD3EAP	1
NM_017432	PTOV1	1
NM_173475	DCUN1D3	1
NM_138422	LOC113179	1
NM_207320	OTUD6A	1
NM_001101676		1
NM_003162	STRN	1
NM_058197	CDKN2A	1
NM_058195	CDKN2A	1
NM_000077	CDKN2A	1
NM_181489	ZNF445	1
NM_005502	ABCA1	1
XM_001128243	LOC728705	1
NM_021964	ZNF148	1
NM_003918	GYG2	1
NM_001079855	GYG2	1
NM_198243	ASB7	1
NM_002268	KPNA4	1
NM_001009610		1
NM_001003940	BMF	1
NM_001003943	BMF	1
NM_001003942	BMF	1
NM_033503	BMF	1
NM_004651	USP11	1
NM_005026	PIK3CD	1
NM_000602	SERPINE1	1
NM_145867	LTC4S	1
NM_015046	SETX	1
NM_138809	CMBL	1
NM_153675	FOXA2	1
NM_021784	FOXA2	1
NM_024733	ZNF665	1
NM_007110	TEP1	1
NM_014764	DAZAP2	1
NM_020856	TSHZ3	1
NM_005088	CXYorf3	1
NM_207118	GTF2H5	1
NM_139279	MCFD2	1
NM_001105543		1
NM_182729	TXNRD1	1
NM_001093771		1
NM_182742	TXNRD1	1
NM_003330	TXNRD1	1
NM_182743	TXNRD1	1
NM_020936	DDX55	1
NM_015541	LRIG1	1
NM_032204	ASCC2	1
XM_001126195	LOC727942	1
NM_032028	TSSK1B	1
NM_203422	LOC221091	1
XM_001130636	LOC221091	1
NM_032431	SYVN1	1
NM_172230	SYVN1	1
NM_024516	C16orf53	1
NM_001099439		1
NM_003482	MLL2	1
NM_001042616	PIGY	1
NM_032906	PIGY	1
NM_016339	RAPGEFL1	1
NM_014047	C19orf53	1
NM_024069	C19orf50	1
NM_024738	C12orf49	1
NM_014692		1
NM_006371	CRTAP	1
NM_002111	HD	1
XM_942822	LOC646626	1
XM_933781	LOC646626	1
NM_024721	ZFHX4	1
NM_052855	ANKRD40	1
NM_021646	ZNF500	1
XM_943522	LOC441005	1
XM_498961	LOC441005	1
NM_194460	RNF126	1
XM_001125986	LOC283824	1
NM_022166	XYLT1	1
NM_175609	ARFGAP1	1
NM_018209	ARFGAP1	1
NM_017515	SLC35F2	1
NM_004430	EGR3	1
XM_001130047	EGR3	1
NM_181491	SURF5	1
NM_001098531		1
NM_006105	RAPGEF3	1
NM_001098532		1
NM_000911	OPRD1	1
XM_946032	LOC644852	1
XM_934218	LOC644852	1
NM_181804	PKIG	1
NM_181805	PKIG	1
NM_007066	PKIG	1
NM_000704	ATP4A	1
NM_001440	EXTL3	1
NM_178519	C17orf55	1
NM_002878	RAD51L3	1
NM_133629	RAD51L3	1
NM_174953	ATP2A3	1
NM_174956	ATP2A3	1
NM_174954	ATP2A3	1
NM_174957	ATP2A3	1
NM_174958	ATP2A3	1
NM_005173	ATP2A3	1
NM_174955	ATP2A3	1
NM_006307	SRPX	1
XM_931281	LOC643072	1
XM_943769	LOC643072	1
NM_014012	REM1	1
NM_198154	TMEM95	1
NM_001010866	RP13-15M17.2	1
NM_025195	TRIB1	1
XM_001133576	LOC729558	1
NM_002180	IGHMBP2	1
NM_175895	C12orf61	1
NM_000901	NR3C2	1
XM_001131014	LOC729683	1
XM_001130799	LOC731783	1
NM_003018	SFTPC	1
NM_001080830	PRAMEF12	1
NM_030589	CYP2A7	1
NM_000764	CYP2A7	1
NM_052906	LRRC62	1
NM_001111019		1
NM_145117	NAV2	1
NM_182964	NAV2	1
NM_001111018		1
NM_153347	TMEM86A	1
NM_002332	LRP1	1
NM_015247	CYLD	1
NM_001042412	CYLD	1
NM_001042355	CYLD	1
NM_145115	ZNF498	1
NM_003936	CDK5R2	1
NM_016563	RASL12	1
NM_133625	SYN2	1
NM_053056	CCND1	1
NM_139177	SLC39A11	1
NM_020705	TBC1D24	1
NM_000738	CHRM1	1
NM_080552	SLC32A1	1
NM_001048209	HNT	1
NM_016522	HNT	1
NM_032853	MUM1	1
NM_024760	TLE6	1
XM_938935	NXPH4	1
NM_007224	NXPH4	1
NM_207348	SLC25A34	1
NM_014840	NUAK1	1
NM_005453	ZBTB22	1
NM_145644	MRPL35	1
NM_016622	MRPL35	1
XM_941762	SETD1B	1
XM_037523	SETD1B	1
NM_018027	FRMD4A	1
XM_001130884	LOC729639	1
XM_001130664	LOC731756	1
NM_145068	TRPV3	1
NM_002938	RNF4	1
XM_001129151	LOC729047	1
NM_016334	GPR89A	1
NM_001097616		1
XM_498479	LOC439962	1
NM_152272	CHMP7	1
XM_931359	LOC338758	1
XM_944677	LOC338758	1
NM_003507	FZD7	1
NM_021961	TEAD1	1
NM_032932	RAB11FIP4	1
XM_938519	DKFZP434I0714	1
XM_929673	DKFZP434I0714	1
NM_015530	GORASP2	1
NM_172115	CAMK2D	1
NM_025074	FRAS1	1
NM_139179	DAGLB	1
NM_005335	HCLS1	1
NM_005327	HADH	1
NM_000033	ABCD1	1
NM_032152	PRAM1	1
NM_178136	POLDIP3	1
NM_032311	POLDIP3	1
NM_014636	RALGPS1	1
NM_152219	GJC1	1
NM_005601	NKG7	1
XM_942869	BOAT	1
XM_292512	BOAT	1
NM_001040424	PRDM15	1
XM_945887	LOC644090	1
NM_012279	ZNF346	1
XM_001132013	ANKH	1
NM_021216	ZNF71	1
NM_080415	SEPT4	1
NM_015074	KIF1B	1
NM_130848	C5orf20	1
NM_080725	SRXN1	1
NM_001128	AP1G1	1
NM_001030007	AP1G1	1
NM_001093725		1
NM_004309	ARHGDIA	1
NM_012388	PLDN	1
NM_183238	ZNF605	1
NM_033224	PURB	1
NM_004442	EPHB2	1
NM_017449	EPHB2	1
NM_032607	CREB3L3	1
NM_021021	SNTB1	1
NM_005667	RNF103	1
NM_020132	AGPAT3	1
NM_001037553	AGPAT3	1
NM_007033	RER1	1
NM_015178	RHOBTB2	1
NM_007286	SYNPO	1
NM_145803	TRAF6	1
NM_004620	TRAF6	1
NM_016932	SIX2	1
NM_052936	ATG4A	1
NM_178270	ATG4A	1
NM_016262	TUBE1	1
NM_079837	BANP	1
NM_017869	BANP	1
NM_015215	CAMTA1	1
NM_024819	DCAKD	1
NM_021945	C6orf85	1
XM_001128351	LOC728288	1
XM_001128438	LOC730770	1
XM_001133706	LOC731533	1
NM_018423	STYK1	1
NM_014862	ARNT2	1
NM_199131	VAX1	1
NM_002698	POU2F2	1
XM_001133467	PKD1L3	1
XM_001134223	PKD1L3	1
NM_001105529		1
NM_006095	ATP8A1	1
NM_174918	C19orf59	1
NM_014393	STAU2	1
NM_015318	ARHGEF18	1
NM_003428	ZNF84	1
NM_006047	RBM12	1
NM_152838	RBM12	1
NM_003636	KCNAB2	1
NM_172130	KCNAB2	1
NM_001112800		1
NM_021097	SLC8A1	1
NM_001112801		1
NM_001112802		1
NM_001017392	SFRS14	1
NM_014810	CEP350	1
NM_173582	PGM2L1	1
NM_024770	METTL8	1
NM_016224	SNX9	1
NM_033315	RASL10B	1
NM_144973	MGC24039	1
NM_032855	HSH2D	1
NM_213596	FOXN4	1
NM_001044387	ZNF557	1
NM_024341	ZNF557	1
NM_001044388	ZNF557	1
NM_001029858	SLC35F1	1
XM_001131068	LOC729696	1
XM_001129643	LOC731435	1
NM_020901	KIAA1542	1
NM_052909	KIAA1909	1
NM_001040613	TMEM70	1
NM_017866	TMEM70	1
NM_052966	FAM129A	1
NM_001754	RUNX1	1
NM_001001890	RUNX1	1
NM_133443	GPT2	1
NM_001079529	LOC202134	1
NM_015094	HIC2	1
NM_014048	MKL2	1
NM_058172	ANTXR2	1
NM_003356	UCP3	1
NM_001098627		1
NM_032643	IRF5	1
NM_002200	IRF5	1
NM_001098631		1
NM_001098629		1
NM_001098628		1
NM_001098630		1
NM_001040427	HAGH	1
NM_005326	HAGH	1
NM_014675	CROCC	1
NM_001079911	DMP1	1
NM_004407	DMP1	1
XM_942958	LOC642236	1
XM_943005	LOC642236	1
XM_001126144	LOC730510	1
NM_138972	BACE1	1
NM_012104	BACE1	1
NM_138973	BACE1	1
NM_138971	BACE1	1
NM_001006618	MAPKAP1	1
NM_022167	XYLT2	1
NM_001014812	FAM96A	1
NM_005669	REEP5	1
NM_018685	ANLN	1
NM_014553	TFCP2L1	1
XM_937933	LOC646561	1
XM_929496	LOC646561	1
NM_001081563	DMPK	1
NM_004409	DMPK	1
NM_001081560	DMPK	1
NM_001081562	DMPK	1
NM_017662	TRPM6	1
NM_175080	P2RX5	1
NM_002561	P2RX5	1
NM_182481	BAGE3	1
NM_182482	BAGE2	1

---

Immunostimulatory motif search results

UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).

GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).

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Settings used for search

Scores were calculated using scoring scheme SS1
Using AVG scoring mode.

Hit scores

Parameter	Spec. Score cut-off	Colour	Comment
Hit to same gene		Colour	Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit.
High scoring hit	≥80	Colour	Hits are very likely to cause an off-target effect
Medium scoring hit	<80¸≥40	Colour	Hits with a small chance of causing an off target effect.
Low scoring hit		Colour	Hits very unlikely to cause an off-target effect

The mismatches are colour coded based on the weighting of that position.

Cut-off	Colour
Match	Colour
Wobble base pairs (G-U)	Colour
Critical bases (none)	Colour
0.6 bits	Colour
0.4 bits	Colour
0.2 bits	Colour

About SpecificityServer

SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).

Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer

For more details about the algorithm "siRNA specificity searching incorporating mismatch tolerance data" Bioinformatics (2008) 24 (10): 1316-1317