Automatic recommendation
Warning - siRNA is specific for only the antisense (guide) strand
13 hits were found for AUGGCUGCUUAUGGUGACA (3 guide strand hits and 10 sense strand hits.)
Hits to Guide strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_000766 | CYP2A13 | 21 | 39 | A | g | G | G | C | U | G | C | U | U | c | U | G | G | U | G | A | C | c | 22 | 3 | 8 | c | false | <90% | 1747 | ||
| XR_017679 | LOC388707 | 438 | 456 | A | U | G | G | C | U | u | C | U | U | A | U | G | G | U | u | A | C | u | 2 | 3 | 8 | c | false | <90% | 1059 | ||
| XR_015965 | LOC731543 | 438 | 456 | A | U | G | G | C | U | u | C | U | U | A | U | G | G | U | u | A | C | u | 2 | 3 | 8 | c | false | <90% | 1059 | ||
Hits to Sense strand
| Accession | Gene | Start | End | Details of mismatches | Spec. Score | Mismatches | Contiguous | Jackson style specificity | Hits similar gene | HSP Score | HSP % Identity | HSP length | Gene Length | ||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | |||||||||||||
| NM_005647 | TBL1X | 595 | 613 | a | G | U | C | A | C | C | A | U | A | A | G | g | A | G | C | C | c | U | 84 | 3 | 11 | false | <90% | 5886 | |||
| NM_152491 | FLJ32569 | 2111 | 2129 | U | G | U | C | A | C | C | A | U | A | A | u | C | A | G | u | C | A | a | 35 | 3 | 11 | false | <90% | 2265 | |||
| NM_025112 | ZXDC | 439 | 457 | c | G | U | C | A | C | C | A | U | c | A | G | C | A | G | C | C | A | g | 10 | 3 | 8 | bc | false | <90% | 3410 | ||
| NM_001040653 | ZXDC | 439 | 457 | c | G | U | C | A | C | C | A | U | c | A | G | C | A | G | C | C | A | g | 10 | 3 | 8 | bc | false | <90% | 4888 | ||
| NM_001081752 | ELN | 1162 | 1180 | U | G | U | C | A | C | C | A | g | A | A | G | C | A | G | C | u | g | c | 8 | 4 | 8 | c | false | <90% | 3339 | ||
| NM_001081753 | ELN | 1207 | 1225 | U | G | U | C | A | C | C | A | g | A | A | G | C | A | G | C | u | g | c | 8 | 4 | 8 | c | false | <90% | 3384 | ||
| NM_001081755 | ELN | 1192 | 1210 | U | G | U | C | A | C | C | A | g | A | A | G | C | A | G | C | u | g | c | 8 | 4 | 8 | c | false | <90% | 3423 | ||
| NM_001081754 | ELN | 1207 | 1225 | U | G | U | C | A | C | C | A | g | A | A | G | C | A | G | C | u | g | c | 8 | 4 | 8 | c | false | <90% | 3441 | ||
| NM_000501 | ELN | 1192 | 1210 | U | G | U | C | A | C | C | A | g | A | A | G | C | A | G | C | u | g | c | 8 | 4 | 8 | c | false | <90% | 3480 | ||
| NM_018036 | C14orf103 | 4853 | 4871 | U | u | g | C | A | C | C | A | a | A | A | G | C | A | G | C | C | A | U | 8 | 3 | 10 | bc | false | <90% | 11099 | ||
3 prime UTR Seed matches
Hits to same gene : 0
Hits to other genes : 2530
View matched accessions
3 prime seed match details
Immunostimulatory motif search results
UGUGU - Judge et al. Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 2005 Apr;23(4):457-62. (PMID: 15778705)
No Immunostimulatory motif found (UGUGU).
GUCCUUCAA - Hornung et al. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 2005 Mar;11(3):263-70. Epub 2005 Feb 20. (PMID: 15723075)
No Immunostimulatory motif found (GUCCUUCAA).
Settings used for search
Scores were calculated using scoring scheme SS1
Using AVG scoring mode.
Hit scores
| Parameter | Spec. Score cut-off | Colour | Comment |
| Hit to same gene | Colour | Hits to the same accession or genes with 100 percent identity (i.e. likely transcript variants). It is advisable to always check the genes hit. | |
| High scoring hit | ≥80 | Colour | Hits are very likely to cause an off-target effect |
| Medium scoring hit | <80¸≥40 | Colour | Hits with a small chance of causing an off target effect. |
| Low scoring hit | Colour | Hits very unlikely to cause an off-target effect |
The mismatches are colour coded based on the weighting of that position.
| Cut-off | Colour |
| Match | Colour |
| Wobble base pairs (G-U) | Colour |
| Critical bases (none) | Colour |
| 0.6 bits | Colour |
| 0.4 bits | Colour |
| 0.2 bits | Colour |
SpecificityServer is designed to help you identify potential non-specific matches to your siRNA. It incorporates the latest information about non-specific matches (sequence-specific only).
Designed by: Alistair Chalk, Quan Du, Zicai Liang and Erik L.L. Sonnhammer