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Page - 1 of 1 * siRNA Target Site Conservation Among 1496 HIV Reference Genome HIVsir ID HIV Strain NCBI Accession Target Gene Position of siRNA target Sense Sequence of siRNA Length of siRNA Efficacy (%) GC Content (%) Cell line siRNA Source Transfection Reagent Test Objective Test method Test Time(Hours) Target Conservation * HIV Esc Seq PubMed 1608 HIV-1 (NL4-3) AF324493 rev K*8509 GCCUGUGCCUCUUCAGCUACC 21 95.00 50 293 T shRNA Lipofectamine 2000 Luc Activity Dual luciferase assay **6 DAYS 675(45.12%) 1608 18596982

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