Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_060501                                                              17    3301
NP_000284                                                              17    4312
NP_001027005                                                           17    4312
XP_005256041                                                           17    4312
XP_005256040                                                           17    4312
NP_003238                                                              16    5631
NP_079335                                                              16    5631
XP_006715631                                                           16    5631
NP_009016                                                              16    7355
NP_001026                                                              15    9605
NP_055968                                                              15    9605
NP_060136                                                              15    9605
NP_061027                                                              15    9605
NP_001277188                                                           15    9605
NP_001167571                                                           15    9605
NP_127503                                                              15    9605
NP_127501                                                              15    9605
NP_065151                                                              15    9605
XP_006724828                                                           15    9605
XP_006715490                                                           15    9605
XP_005248760                                                           15    9605
XP_005248759                                                           15    9605
XP_006715489                                                           15    9605
XP_005248758                                                           15    9605
XP_006713333                                                           15    9605
XP_005265389                                                           15    9605
XP_005265388                                                           15    9605
XP_006713332                                                           15    9605
XP_006713331                                                           15    9605
XP_006713330                                                           15    9605
XP_006713329                                                           15    9605
XP_005265387                                                           15    9605
XP_006711904                                                           15    9605
XP_006711873                                                           15    9605
XP_006711872                                                           15    9605
XP_006711871                                                           15    9605
XP_006711870                                                           15    9605
XP_006711869                                                           15    9605
XP_006711868                                                           15    9605
XP_006711867                                                           15    9605
XP_006711866                                                           15    9605
XP_006711865                                                           15    9605
>NP_060501
          Length = 1151

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  CLSTSKI 8
          CLSTS+I
Sbjct: 23 CLSTSRI 29
>NP_000284
          Length = 1093

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   CLSTSKI 8
           CL TSKI
Sbjct: 559 CLGTSKI 565
>NP_001027005
          Length = 1086

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   CLSTSKI 8
           CL TSKI
Sbjct: 552 CLGTSKI 558
>XP_005256041
          Length = 1086

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   CLSTSKI 8
           CL TSKI
Sbjct: 552 CLGTSKI 558
>XP_005256040
          Length = 1093

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   CLSTSKI 8
           CL TSKI
Sbjct: 559 CLGTSKI 565
>NP_003238
          Length = 1172

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   CLSTSKI 8
           C STSK+
Sbjct: 601 CFSTSKV 607
>NP_079335
          Length = 715

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   ACLSTSKI 8
           A LSTSKI
Sbjct: 113 AALSTSKI 120
>XP_006715631
          Length = 648

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   ACLSTSKI 8
           A LSTSKI
Sbjct: 113 AALSTSKI 120
>NP_009016
          Length = 308

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  ACLSTSKI 8
          ACL+ SKI
Sbjct: 83 ACLTGSKI 90
>NP_001026
          Length = 4967

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   CLSTSK 7
           CLSTS+
Sbjct: 132 CLSTSR 137
>NP_055968
          Length = 2039

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    ACLSTSKI 8
            AC STS I
Sbjct: 1357 ACASTSHI 1364
>NP_060136
          Length = 737

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   CLSTSKIL 9
           C S SK+L
Sbjct: 318 CFSCSKVL 325
>NP_061027
          Length = 4599

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  ACLSTSK 7
          ACL T+K
Sbjct: 83 ACLGTNK 89
>NP_001277188
          Length = 1951

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    ACLSTSKI 8
            AC STS I
Sbjct: 1269 ACASTSHI 1276
>NP_001167571
          Length = 403

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ACLSTSK 7
           ACL T+K
Sbjct: 307 ACLETAK 313
>NP_127503
          Length = 403

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ACLSTSK 7
           ACL T+K
Sbjct: 307 ACLETAK 313
>NP_127501
          Length = 403

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ACLSTSK 7
           ACL T+K
Sbjct: 307 ACLETAK 313
>NP_065151
          Length = 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ACLSTSK 7
           ACL T+K
Sbjct: 264 ACLETAK 270
>XP_006724828
          Length = 688

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   CLSTSKIL 9
           C S SK+L
Sbjct: 318 CFSCSKVL 325
>XP_006715490
          Length = 1181

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   ACLSTSKI 8
           AC STS I
Sbjct: 499 ACASTSHI 506
>XP_005248760
          Length = 1308

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   ACLSTSKI 8
           AC STS I
Sbjct: 626 ACASTSHI 633
>XP_005248759
          Length = 1951

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    ACLSTSKI 8
            AC STS I
Sbjct: 1269 ACASTSHI 1276
>XP_006715489
          Length = 2039

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    ACLSTSKI 8
            AC STS I
Sbjct: 1357 ACASTSHI 1364
>XP_005248758
          Length = 2039

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    ACLSTSKI 8
            AC STS I
Sbjct: 1357 ACASTSHI 1364
>XP_006713333
          Length = 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ACLSTSK 7
           ACL T+K
Sbjct: 264 ACLETAK 270
>XP_005265389
          Length = 383

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ACLSTSK 7
           ACL T+K
Sbjct: 287 ACLETAK 293
>XP_005265388
          Length = 403

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ACLSTSK 7
           ACL T+K
Sbjct: 307 ACLETAK 313
>XP_006713332
          Length = 403

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ACLSTSK 7
           ACL T+K
Sbjct: 307 ACLETAK 313
>XP_006713331
          Length = 403

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ACLSTSK 7
           ACL T+K
Sbjct: 307 ACLETAK 313
>XP_006713330
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ACLSTSK 7
           ACL T+K
Sbjct: 328 ACLETAK 334
>XP_006713329
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ACLSTSK 7
           ACL T+K
Sbjct: 328 ACLETAK 334
>XP_005265387
          Length = 426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ACLSTSK 7
           ACL T+K
Sbjct: 330 ACLETAK 336
>XP_006711904
          Length = 630

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  ACLSTSK 7
          +CLSTS+
Sbjct: 38 SCLSTSQ 44
>XP_006711873
          Length = 4954

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   CLSTSK 7
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711872
          Length = 2735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   CLSTSK 7
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711871
          Length = 4906

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   CLSTSK 7
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711870
          Length = 4965

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   CLSTSK 7
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711869
          Length = 4973

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   CLSTSK 7
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711868
          Length = 4975

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   CLSTSK 7
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711867
          Length = 4977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   CLSTSK 7
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711866
          Length = 4984

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   CLSTSK 7
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711865
          Length = 4985

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   CLSTSK 7
           CLSTS+
Sbjct: 132 CLSTSR 137
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.125    0.348 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 531,394
Number of extensions: 707
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package