Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001268421                                                           18    1135
NP_000170                                                              18    1135
NP_061928                                                              16    5631
NP_803879                                                              16    5631
NP_803881                                                              16    5631
NP_001258992                                                           16    5631
NP_110428                                                              16    5631
NP_001229291                                                           16    5631
NP_001258991                                                           16    5631
NP_001092270                                                           16    5631
NP_001258990                                                           16    5631
XP_006724671                                                           16    5631
XP_006724670                                                           16    5631
XP_005271654                                                           16    5631
XP_005271653                                                           16    5631
XP_006718924                                                           16    5631
NP_073594                                                              16    7355
XP_005247773                                                           16    7355
XP_006713796                                                           16    7355
XP_005247772                                                           16    7355
XP_005247768                                                           16    7355
XP_006713794                                                           16    7355
XP_005247767                                                           16    7355
>NP_001268421
          Length = 1230

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  ANEASARAS 9
          AN+ASARAS
Sbjct: 20 ANKASARAS 28
>NP_000170
          Length = 1360

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  ANEASARAS 9
          AN+ASARAS
Sbjct: 20 ANKASARAS 28
>NP_061928
          Length = 1007

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   NEASARAS 9
           N ASARAS
Sbjct: 884 NAASARAS 891
>NP_803879
          Length = 741

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ANEASARAS 9
           ANE +ARA+
Sbjct: 97  ANETAARAA 105
>NP_803881
          Length = 739

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ANEASARAS 9
           ANE +ARA+
Sbjct: 97  ANETAARAA 105
>NP_001258992
          Length = 741

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ANEASARAS 9
           ANE +ARA+
Sbjct: 97  ANETAARAA 105
>NP_110428
          Length = 741

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ANEASARAS 9
           ANE +ARA+
Sbjct: 97  ANETAARAA 105
>NP_001229291
          Length = 757

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ANEASARAS 9
           ANE +ARA+
Sbjct: 97  ANETAARAA 105
>NP_001258991
          Length = 741

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ANEASARAS 9
           ANE +ARA+
Sbjct: 97  ANETAARAA 105
>NP_001092270
          Length = 739

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ANEASARAS 9
           ANE +ARA+
Sbjct: 97  ANETAARAA 105
>NP_001258990
          Length = 757

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ANEASARAS 9
           ANE +ARA+
Sbjct: 97  ANETAARAA 105
>XP_006724671
          Length = 741

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ANEASARAS 9
           ANE +ARA+
Sbjct: 97  ANETAARAA 105
>XP_006724670
          Length = 741

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ANEASARAS 9
           ANE +ARA+
Sbjct: 97  ANETAARAA 105
>XP_005271654
          Length = 862

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   NEASARAS 9
           N ASARAS
Sbjct: 739 NAASARAS 746
>XP_005271653
          Length = 862

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   NEASARAS 9
           N ASARAS
Sbjct: 739 NAASARAS 746
>XP_006718924
          Length = 969

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   NEASARAS 9
           N ASARAS
Sbjct: 846 NAASARAS 853
>NP_073594
          Length = 912

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   NEASARA 8
           NEASAR+
Sbjct: 135 NEASARS 141
>XP_005247773
          Length = 523

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   NEASARA 8
           NEASAR+
Sbjct: 176 NEASARS 182
>XP_006713796
          Length = 651

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   NEASARA 8
           NEASAR+
Sbjct: 176 NEASARS 182
>XP_005247772
          Length = 700

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   NEASARA 8
           NEASAR+
Sbjct: 176 NEASARS 182
>XP_005247768
          Length = 794

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   NEASARA 8
           NEASAR+
Sbjct: 176 NEASARS 182
>XP_006713794
          Length = 810

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   NEASARA 8
           NEASAR+
Sbjct: 176 NEASARS 182
>XP_005247767
          Length = 953

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   NEASARA 8
           NEASAR+
Sbjct: 176 NEASARS 182
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.281   0.0873    0.187 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 173,403
Number of extensions: 85
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.9 bits)
S2: 28 (15.4 bits)

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