Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001177396                                                           17    3301
NP_004806                                                              17    4312
XP_006711111                                                           17    4312
NP_060264                                                              16    7355
NP_060743                                                              16    7355
XP_005251551                                                           16    7355
XP_006716857                                                           16    7355
NP_001116848                                                           16    7355
NP_001234                                                              15    9605
NP_001268359                                                           15    9605
NP_001035987                                                           15    9605
NP_056007                                                              15    9605
XP_006723282                                                           15    9605
XP_006711112                                                           15    9605
NP_542401                                                              15    9605
>NP_001177396
          Length = 1346

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   APLALSSL 8
           +PLALSSL
Sbjct: 604 SPLALSSL 611
>NP_004806
          Length = 1261

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   APLALSSLA 9
           AP+ +SSLA
Sbjct: 858 APITISSLA 866
>XP_006711111
          Length = 1197

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   APLALSSLA 9
           AP+ +SSLA
Sbjct: 794 APITISSLA 802
>NP_060264
          Length = 1801

 Score = 15.8 bits (29), Expect =  7355
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   APLALSSLA 9
           A LALSSLA
Sbjct: 960 ALLALSSLA 968
>NP_060743
          Length = 459

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  PLALSSLA 9
          P ALSSLA
Sbjct: 41 PRALSSLA 48
>XP_005251551
          Length = 1801

 Score = 15.8 bits (29), Expect =  7355
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   APLALSSLA 9
           A LALSSLA
Sbjct: 960 ALLALSSLA 968
>XP_006716857
          Length = 1834

 Score = 15.8 bits (29), Expect =  7355
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    APLALSSLA 9
            A LALSSLA
Sbjct: 993  ALLALSSLA 1001
>NP_001116848
          Length = 275

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   APLALSSL 8
           APL LSSL
Sbjct: 262 APLFLSSL 269
>NP_001234
          Length = 595

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   APLALSS 7
           AP+ALSS
Sbjct: 370 APVALSS 376
>NP_001268359
          Length = 483

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   APLALSS 7
           AP+ALSS
Sbjct: 259 APVALSS 265
>NP_001035987
          Length = 410

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   APLALSS 7
           APLALS+
Sbjct: 230 APLALST 236
>NP_056007
          Length = 1216

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    APLALSS 7
            APL+LSS
Sbjct: 1182 APLSLSS 1188
>XP_006723282
          Length = 410

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   APLALSS 7
           APLALS+
Sbjct: 230 APLALST 236
>XP_006711112
          Length = 545

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   APLALSS 7
           AP+ALSS
Sbjct: 321 APVALSS 327
>NP_542401
          Length = 324

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  APLALSSL 8
          AP ALS+L
Sbjct: 87 APFALSAL 94
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.119    0.291 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 386,587
Number of extensions: 601
Number of successful extensions: 15
Number of sequences better than 10000.0: 15
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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