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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001264198                                                           17    3301
XP_005265796                                                           17    3301
NP_001019782                                                           17    3301
NP_004781                                                              16    5631
NP_695008                                                              16    5631
NP_695010                                                              16    5631
NP_695009                                                              16    5631
NP_149076                                                              16    5631
NP_938023                                                              16    7355
XP_005268502                                                           16    7355
NP_149024                                                              15    9605
NP_004949                                                              15    9605
NP_542164                                                              15    9605
NP_001275707                                                           15    9605
NP_001275705                                                           15    9605
NP_001275505                                                           15    9605
NP_001096645                                                           15    9605
NP_001093881                                                           15    9605
NP_116019                                                              15    9605
NP_004415                                                              15    9605
NP_078857                                                              15    9605
XP_006722446                                                           15    9605
XP_006722445                                                           15    9605
XP_006722444                                                           15    9605
XP_006722443                                                           15    9605
XP_006720921                                                           15    9605
XP_006720920                                                           15    9605
XP_005255213                                                           15    9605
XP_005255212                                                           15    9605
XP_005255192                                                           15    9605
XP_006720913                                                           15    9605
XP_005263495                                                           15    9605

>NP_001264198
          Length = 2925

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LAKELTH 9
           LAKE+TH
Sbjct: 222 LAKEVTH 228



>XP_005265796
          Length = 880

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   ASLAKELTH 9
           ASL K LTH
Sbjct: 586 ASLCKMLTH 594



>NP_001019782
          Length = 880

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   ASLAKELTH 9
           ASL K LTH
Sbjct: 586 ASLCKMLTH 594



>NP_004781
          Length = 563

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   ASLAKELT 8
           ASL KELT
Sbjct: 311 ASLQKELT 318



>NP_695008
          Length = 550

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   ASLAKELT 8
           ASL KELT
Sbjct: 311 ASLQKELT 318



>NP_695010
          Length = 519

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   ASLAKELT 8
           ASL KELT
Sbjct: 311 ASLQKELT 318



>NP_695009
          Length = 506

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   ASLAKELT 8
           ASL KELT
Sbjct: 311 ASLQKELT 318



>NP_149076
          Length = 183

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  SLAKELTH 9
          SLA+EL H
Sbjct: 15 SLAEELNH 22



>NP_938023
          Length = 379

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  SLAKELTH 9
          SLA+EL H
Sbjct: 65 SLAEELRH 72



>XP_005268502
          Length = 283

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  SLAKELTH 9
          SLA+EL H
Sbjct: 65 SLAEELRH 72



>NP_149024
          Length = 159

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  ASLAKEL 7
          ASLAKE+
Sbjct: 91 ASLAKEM 97



>NP_004949
          Length = 2549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  ASLAKELTH 9
          A  AKEL H
Sbjct: 38 AKAAKELQH 46



>NP_542164
          Length = 950

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AKELTH 9
           AKE+TH
Sbjct: 117 AKEVTH 122



>NP_001275707
          Length = 607

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ASLAKELTH 9
           ASLA +++H
Sbjct: 125 ASLAADISH 133



>NP_001275705
          Length = 654

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ASLAKELTH 9
           ASLA +++H
Sbjct: 125 ASLAADISH 133



>NP_001275505
          Length = 936

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   ASLAKELTH 9
           ASL  EL H
Sbjct: 699 ASLRSELNH 707



>NP_001096645
          Length = 508

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   ASLAKELTH 9
           ASL  EL H
Sbjct: 293 ASLQSELAH 301



>NP_001093881
          Length = 971

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   ASLAKELTH 9
           ASL  EL H
Sbjct: 699 ASLRSELNH 707



>NP_116019
          Length = 212

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SLAKELTH 9
           ++AKEL H
Sbjct: 165 TVAKELAH 172



>NP_004415
          Length = 784

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ASLAKELTH 9
           ASLA +++H
Sbjct: 125 ASLAADISH 133



>NP_078857
          Length = 1140

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   ASLAKELTH 9
           ASL  EL H
Sbjct: 819 ASLRSELNH 827



>XP_006722446
          Length = 889

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AKELTH 9
           AKE+TH
Sbjct: 117 AKEVTH 122



>XP_006722445
          Length = 905

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AKELTH 9
           AKE+TH
Sbjct: 117 AKEVTH 122



>XP_006722444
          Length = 914

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AKELTH 9
           AKE+TH
Sbjct: 117 AKEVTH 122



>XP_006722443
          Length = 925

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  AKELTH 9
          AKE+TH
Sbjct: 92 AKEVTH 97



>XP_006720921
          Length = 783

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ASLAKELTH 9
           ASLA +++H
Sbjct: 125 ASLAADISH 133



>XP_006720920
          Length = 785

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ASLAKELTH 9
           ASLA +++H
Sbjct: 126 ASLAADISH 134



>XP_005255213
          Length = 836

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ASLAKELTH 9
           ASLA +++H
Sbjct: 178 ASLAADISH 186



>XP_005255212
          Length = 837

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ASLAKELTH 9
           ASLA +++H
Sbjct: 178 ASLAADISH 186



>XP_005255192
          Length = 508

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   ASLAKELTH 9
           ASL  EL H
Sbjct: 293 ASLQSELAH 301



>XP_006720913
          Length = 508

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   ASLAKELTH 9
           ASL  EL H
Sbjct: 293 ASLQSELAH 301



>XP_005263495
          Length = 2549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  ASLAKELTH 9
          A  AKEL H
Sbjct: 38 AKAAKELQH 46



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.305    0.113    0.280 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 465,000
Number of extensions: 733
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)