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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              18    1135
XP_005262424                                                           18    1135
NP_001180251                                                           16    5631
NP_001180250                                                           16    5631
NP_054721                                                              16    5631
NP_060143                                                              16    5631
XP_006711704                                                           16    5631
XP_005273413                                                           16    5631
XP_006711455                                                           16    5631
XP_006711454                                                           16    5631
XP_005245330                                                           16    5631
XP_005245328                                                           16    5631
NP_001098988                                                           16    5631
NP_000341                                                              16    7355
XP_006726589                                                           16    7355
XP_006718462                                                           16    7355
XP_006710010                                                           16    7355
NP_056494                                                              15    9605
NP_037490                                                              15    9605
NP_795362                                                              15    9605

>NP_722576
          Length = 3080

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EITLFSTSV 9
            E TLFSTSV
Sbjct: 1125 ETTLFSTSV 1133



>XP_005262424
          Length = 2875

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EITLFSTSV 9
           E TLFSTSV
Sbjct: 920 ETTLFSTSV 928



>NP_001180251
          Length = 657

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ITLFSTSV 9
           +TLFST++
Sbjct: 379 LTLFSTAI 386



>NP_001180250
          Length = 631

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ITLFSTSV 9
           +TLFST++
Sbjct: 294 LTLFSTAI 301



>NP_054721
          Length = 716

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ITLFSTSV 9
           +TLFST++
Sbjct: 379 LTLFSTAI 386



>NP_060143
          Length = 900

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EITLFSTS 8
           EIT+FS+S
Sbjct: 737 EITVFSSS 744



>XP_006711704
          Length = 646

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ITLFSTSV 9
           +TLFST++
Sbjct: 379 LTLFSTAI 386



>XP_005273413
          Length = 716

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ITLFSTSV 9
           +TLFST++
Sbjct: 379 LTLFSTAI 386



>XP_006711455
          Length = 907

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EITLFSTS 8
           EIT+FS+S
Sbjct: 744 EITVFSSS 751



>XP_006711454
          Length = 907

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EITLFSTS 8
           EIT+FS+S
Sbjct: 744 EITVFSSS 751



>XP_005245330
          Length = 907

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EITLFSTS 8
           EIT+FS+S
Sbjct: 744 EITVFSSS 751



>XP_005245328
          Length = 907

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EITLFSTS 8
           EIT+FS+S
Sbjct: 744 EITVFSSS 751



>NP_001098988
          Length = 900

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EITLFSTS 8
           EIT+FS+S
Sbjct: 737 EITVFSSS 744



>NP_000341
          Length = 2273

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    EITLFSTSV 9
            EIT+ +TSV
Sbjct: 1670 EITVLTTSV 1678



>XP_006726589
          Length = 931

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ITLFSTS 8
           +TLFSTS
Sbjct: 537 MTLFSTS 543



>XP_006718462
          Length = 4966

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    ITLFSTS 8
            +TLFSTS
Sbjct: 4572 MTLFSTS 4578



>XP_006710010
          Length = 1103

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ITLFSTS 8
           +TLFSTS
Sbjct: 709 MTLFSTS 715



>NP_056494
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  EITLFSTSV 9
          E+TL +TSV
Sbjct: 64 ELTLTATSV 72



>NP_037490
          Length = 663

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EITLFSTSV 9
           E+TLF+ +V
Sbjct: 279 EVTLFTDTV 287



>NP_795362
          Length = 338

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EITLFSTSV 9
           EIT+ STS+
Sbjct: 93  EITISSTSL 101



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.126    0.298 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 322,458
Number of extensions: 307
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)