Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     390
NP_001075109                                                           20     390
NP_001243779                                                           17    2528
NP_596869                                                              17    2528
XP_006712787                                                           17    2528
XP_006712786                                                           17    2528
NP_001254479                                                           17    2528
NP_001578                                                              17    3301
NP_000267                                                              17    3301
XP_005262479                                                           17    3301
XP_005262478                                                           17    3301
XP_006724824                                                           17    3301
XP_006724823                                                           17    3301
NP_477520                                                              16    5631
NP_001008563                                                           16    5631
NP_006667                                                              16    5631
NP_001103773                                                           16    5631
NP_001139266                                                           16    5631
NP_005236                                                              16    5631
NP_009149                                                              16    5631
XP_006717001                                                           16    5631
XP_005251722                                                           16    5631
XP_006714202                                                           16    5631
XP_005262892                                                           16    5631
XP_005262891                                                           16    5631
NP_115977                                                              16    7355
NP_778236                                                              16    7355
NP_055318                                                              16    7355
NP_065987                                                              16    7355
NP_061837                                                              16    7355
XP_005261199                                                           16    7355
XP_005261198                                                           16    7355
XP_006724079                                                           16    7355
XP_006724078                                                           16    7355
XP_006724077                                                           16    7355
XP_005261197                                                           16    7355
XP_005261196                                                           16    7355
XP_005253648                                                           16    7355
NP_689807                                                              15    9605
NP_036385                                                              15    9605
NP_001139406                                                           15    9605
NP_001139405                                                           15    9605
NP_001138762                                                           15    9605
NP_938018                                                              15    9605
NP_001129477                                                           15    9605
NP_001005853                                                           15    9605
NP_001129478                                                           15    9605
NP_443163                                                              15    9605
NP_001171643                                                           15    9605
NP_000810                                                              15    9605
NP_009212                                                              15    9605
XP_006724053                                                           15    9605
XP_006724052                                                           15    9605
XP_005260998                                                           15    9605
XP_006724051                                                           15    9605
XP_005254260                                                           15    9605
XP_005254259                                                           15    9605
XP_006714138                                                           15    9605
XP_005247061                                                           15    9605
NP_780294                                                              15    9605
NP_775486                                                              15    9605
>NP_008835
          Length = 4128

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EKRLSFVVP 9
           EKRLSF VP
Sbjct: 889 EKRLSFAVP 897
>NP_001075109
          Length = 4097

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EKRLSFVVP 9
           EKRLSF VP
Sbjct: 889 EKRLSFAVP 897
>NP_001243779
          Length = 34350

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     EKRLSFVVP 9
             E++LSF VP
Sbjct: 10398 EEKLSFAVP 10406
>NP_596869
          Length = 33423

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    EKRLSFVVP 9
            E++LSF VP
Sbjct: 9471 EEKLSFAVP 9479
>XP_006712787
          Length = 33978

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     EKRLSFVVP 9
             E++LSF VP
Sbjct: 10399 EEKLSFAVP 10407
>XP_006712786
          Length = 35484

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     EKRLSFVVP 9
             E++LSF VP
Sbjct: 10399 EEKLSFAVP 10407
>NP_001254479
          Length = 35991

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     EKRLSFVVP 9
             E++LSF VP
Sbjct: 10715 EEKLSFAVP 10723
>NP_001578
          Length = 893

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RLSFVVP 9
           R+SFVVP
Sbjct: 396 RMSFVVP 402
>NP_000267
          Length = 901

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RLSFVVP 9
           R+SFVVP
Sbjct: 396 RMSFVVP 402
>XP_005262479
          Length = 744

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RLSFVVP 9
           R+SFVVP
Sbjct: 239 RMSFVVP 245
>XP_005262478
          Length = 835

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RLSFVVP 9
           R+SFVVP
Sbjct: 330 RMSFVVP 336
>XP_006724824
          Length = 890

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RLSFVVP 9
           R+SFVVP
Sbjct: 393 RMSFVVP 399
>XP_006724823
          Length = 898

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RLSFVVP 9
           R+SFVVP
Sbjct: 393 RMSFVVP 399
>NP_477520
          Length = 488

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  EKRLSFVV 8
          E RLSF+V
Sbjct: 77 EMRLSFIV 84
>NP_001008563
          Length = 914

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EKRLSFVVP 9
           +KR S+VVP
Sbjct: 202 KKRPSYVVP 210
>NP_006667
          Length = 914

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EKRLSFVVP 9
           +KR S+VVP
Sbjct: 202 KKRPSYVVP 210
>NP_001103773
          Length = 914

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EKRLSFVVP 9
           +KR S+VVP
Sbjct: 202 KKRPSYVVP 210
>NP_001139266
          Length = 489

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  EKRLSFVV 8
          E RLSF+V
Sbjct: 77 EMRLSFIV 84
>NP_005236
          Length = 4588

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    RLSFVVP 9
            RLSFV P
Sbjct: 3766 RLSFVTP 3772
>NP_009149
          Length = 664

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EKRLSFVVP 9
           ++RL FV P
Sbjct: 320 DRRLGFVAP 328
>XP_006717001
          Length = 914

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EKRLSFVVP 9
           +KR S+VVP
Sbjct: 202 KKRPSYVVP 210
>XP_005251722
          Length = 914

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EKRLSFVVP 9
           +KR S+VVP
Sbjct: 202 KKRPSYVVP 210
>XP_006714202
          Length = 4588

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    RLSFVVP 9
            RLSFV P
Sbjct: 3766 RLSFVTP 3772
>XP_005262892
          Length = 4600

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    RLSFVVP 9
            RLSFV P
Sbjct: 3766 RLSFVTP 3772
>XP_005262891
          Length = 4600

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    RLSFVVP 9
            RLSFV P
Sbjct: 3766 RLSFVTP 3772
>NP_115977
          Length = 353

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EKRLSFV 7
           EK+LSF+
Sbjct: 236 EKKLSFI 242
>NP_778236
          Length = 666

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KRLSFVVP 9
           K L+FV+P
Sbjct: 262 KTLAFVIP 269
>NP_055318
          Length = 2785

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    EKRLSFVVP 9
            EKRL  V+P
Sbjct: 2400 EKRLGTVLP 2408
>NP_065987
          Length = 600

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  KRLSFVVP 9
          K L+FV+P
Sbjct: 59 KTLAFVIP 66
>NP_061837
          Length = 533

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LSFVVP 9
           LSFVVP
Sbjct: 221 LSFVVP 226
>XP_005261199
          Length = 429

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  LSFVVP 9
          LSFVVP
Sbjct: 88 LSFVVP 93
>XP_005261198
          Length = 533

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LSFVVP 9
           LSFVVP
Sbjct: 221 LSFVVP 226
>XP_006724079
          Length = 562

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LSFVVP 9
           LSFVVP
Sbjct: 221 LSFVVP 226
>XP_006724078
          Length = 562

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LSFVVP 9
           LSFVVP
Sbjct: 221 LSFVVP 226
>XP_006724077
          Length = 562

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LSFVVP 9
           LSFVVP
Sbjct: 221 LSFVVP 226
>XP_005261197
          Length = 562

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LSFVVP 9
           LSFVVP
Sbjct: 221 LSFVVP 226
>XP_005261196
          Length = 562

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LSFVVP 9
           LSFVVP
Sbjct: 221 LSFVVP 226
>XP_005253648
          Length = 452

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  KRLSFVVP 9
          K L+FV+P
Sbjct: 59 KTLAFVIP 66
>NP_689807
          Length = 444

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LSFVVP 9
           LSF+VP
Sbjct: 410 LSFIVP 415
>NP_036385
          Length = 596

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  KRLSFV 7
          KRLSFV
Sbjct: 84 KRLSFV 89
>NP_001139406
          Length = 540

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  KRLSFV 7
          KRLSFV
Sbjct: 28 KRLSFV 33
>NP_001139405
          Length = 590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  KRLSFV 7
          KRLSFV
Sbjct: 78 KRLSFV 83
>NP_001138762
          Length = 501

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LSFVVP 9
           LSF+VP
Sbjct: 209 LSFIVP 214
>NP_938018
          Length = 505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LSFVVP 9
           LSF+VP
Sbjct: 209 LSFIVP 214
>NP_001129477
          Length = 1010

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  EKRLSFVV 8
          EK++ FVV
Sbjct: 64 EKKIEFVV 71
>NP_001005853
          Length = 312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  EKRLSFV 7
          +KR+SFV
Sbjct: 89 QKRISFV 95
>NP_001129478
          Length = 1010

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  EKRLSFVV 8
          EK++ FVV
Sbjct: 64 EKKIEFVV 71
>NP_443163
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  EKRLSFVVP 9
          E  L+F+VP
Sbjct: 59 ETSLAFIVP 67
>NP_001171643
          Length = 332

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  EKRLSFVVP 9
          E  L+F+VP
Sbjct: 59 ETSLAFIVP 67
>NP_000810
          Length = 1010

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  EKRLSFVV 8
          EK++ FVV
Sbjct: 64 EKKIEFVV 71
>NP_009212
          Length = 98

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3  RLSFVVP 9
          ++SFV+P
Sbjct: 88 KISFVIP 94
>XP_006724053
          Length = 1010

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  EKRLSFVV 8
          EK++ FVV
Sbjct: 64 EKKIEFVV 71
>XP_006724052
          Length = 1010

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  EKRLSFVV 8
          EK++ FVV
Sbjct: 64 EKKIEFVV 71
>XP_005260998
          Length = 1010

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  EKRLSFVV 8
          EK++ FVV
Sbjct: 64 EKKIEFVV 71
>XP_006724051
          Length = 1026

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  EKRLSFVV 8
          EK++ FVV
Sbjct: 80 EKKIEFVV 87
>XP_005254260
          Length = 370

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LSFVVP 9
           LSF+VP
Sbjct: 336 LSFIVP 341
>XP_005254259
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LSFVVP 9
           LSF+VP
Sbjct: 422 LSFIVP 427
>XP_006714138
          Length = 154

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   RLSFVVP 9
           ++SFV+P
Sbjct: 144 KISFVIP 150
>XP_005247061
          Length = 312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  EKRLSFV 7
          +KR+SFV
Sbjct: 89 QKRISFV 95
>NP_780294
          Length = 433

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  EKRLSFVV 8
          EK++ FVV
Sbjct: 64 EKKIEFVV 71
>NP_775486
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  EKRLSFV 7
          +KR+SFV
Sbjct: 89 QKRISFV 95
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.325    0.142    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 511,037
Number of extensions: 905
Number of successful extensions: 61
Number of sequences better than 10000.0: 61
Number of HSP's gapped: 61
Number of HSP's successfully gapped: 61
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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