Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001268421                                                           20     509
NP_000170                                                              20     509
NP_001268422                                                           20     509
NP_001268423                                                           20     509
XP_005264328                                                           20     509
XP_006726469                                                           17    3301
XP_006713516                                                           17    3301
NP_001073291                                                           16    5631
NP_000417                                                              16    5631
XP_005267039                                                           16    5631
XP_005267038                                                           16    5631
NP_848599                                                              16    7355
XP_005255658                                                           16    7355
XP_006713081                                                           16    7355
NP_940978                                                              15    9605
NP_001001132                                                           15    9605
NP_003015                                                              15    9605
NP_689799                                                              15    9605
NP_005877                                                              15    9605
NP_775922                                                              15    9605
XP_005261083                                                           15    9605
XP_005261088                                                           15    9605
XP_005261084                                                           15    9605
XP_006721186                                                           15    9605
XP_005255829                                                           15    9605
XP_006721185                                                           15    9605
XP_006721184                                                           15    9605
XP_005269639                                                           15    9605
XP_006717716                                                           15    9605
XP_006717715                                                           15    9605
XP_006717714                                                           15    9605
XP_006717713                                                           15    9605
XP_006717712                                                           15    9605
XP_006717711                                                           15    9605
XP_005269635                                                           15    9605
XP_006717710                                                           15    9605
XP_006717709                                                           15    9605
XP_006717708                                                           15    9605
XP_006710700                                                           15    9605
XP_006710699                                                           15    9605
XP_006710698                                                           15    9605
XP_005244806                                                           15    9605
XP_006710697                                                           15    9605
XP_006710696                                                           15    9605
>NP_001268421
          Length = 1230

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GASDRTMSG 9
            GASDR MSG
Sbjct: 1048 GASDRIMSG 1056
>NP_000170
          Length = 1360

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GASDRTMSG 9
            GASDR MSG
Sbjct: 1178 GASDRIMSG 1186
>NP_001268422
          Length = 1058

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GASDRTMSG 9
           GASDR MSG
Sbjct: 876 GASDRIMSG 884
>NP_001268423
          Length = 1058

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GASDRTMSG 9
           GASDR MSG
Sbjct: 876 GASDRIMSG 884
>XP_005264328
          Length = 1261

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GASDRTMSG 9
            GASDR MSG
Sbjct: 1079 GASDRIMSG 1087
>XP_006726469
          Length = 175

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GASDRTMS 8
           GA DRT+S
Sbjct: 162 GAEDRTLS 169
>XP_006713516
          Length = 263

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GASDRTMS 8
           GA DRT+S
Sbjct: 250 GAEDRTLS 257
>NP_001073291
          Length = 3118

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    GASDRTMS 8
            GAS+RT+S
Sbjct: 2861 GASNRTIS 2868
>NP_000417
          Length = 3122

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    GASDRTMS 8
            GAS+RT+S
Sbjct: 2865 GASNRTIS 2872
>XP_005267039
          Length = 3206

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    GASDRTMS 8
            GAS+RT+S
Sbjct: 2949 GASNRTIS 2956
>XP_005267038
          Length = 3210

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    GASDRTMS 8
            GAS+RT+S
Sbjct: 2953 GASNRTIS 2960
>NP_848599
          Length = 3092

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    ASDRTMSG 9
            A+D++MSG
Sbjct: 2514 ANDKSMSG 2521
>XP_005255658
          Length = 470

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  GASDRTMSG 9
          GAS RT+ G
Sbjct: 69 GASGRTVCG 77
>XP_006713081
          Length = 3964

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    ASDRTMSG 9
            A+D++MSG
Sbjct: 3386 ANDKSMSG 3393
>NP_940978
          Length = 2045

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    GASDRTMSG 9
            G+SD  +SG
Sbjct: 1065 GSSDEELSG 1073
>NP_001001132
          Length = 1220

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   DRTMSG 9
           D+TMSG
Sbjct: 233 DKTMSG 238
>NP_003015
          Length = 1721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   DRTMSG 9
           D+TMSG
Sbjct: 233 DKTMSG 238
>NP_689799
          Length = 1684

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    GASDRTMSG 9
            G  DR++SG
Sbjct: 1149 GFKDRSLSG 1157
>NP_005877
          Length = 655

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GASDRTM 7
           G+SDRT+
Sbjct: 207 GSSDRTI 213
>NP_775922
          Length = 6996

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    GASDRTMSG 9
            G+ D+TM G
Sbjct: 3341 GSFDQTMKG 3349
>XP_005261083
          Length = 1716

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   DRTMSG 9
           D+TMSG
Sbjct: 233 DKTMSG 238
>XP_005261088
          Length = 1144

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   DRTMSG 9
           D+TMSG
Sbjct: 233 DKTMSG 238
>XP_005261084
          Length = 1215

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   DRTMSG 9
           D+TMSG
Sbjct: 233 DKTMSG 238
>XP_006721186
          Length = 658

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GASDRTM 7
           G+SDRT+
Sbjct: 207 GSSDRTI 213
>XP_005255829
          Length = 672

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GASDRTM 7
           G+SDRT+
Sbjct: 207 GSSDRTI 213
>XP_006721185
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GASDRTM 7
           G+SDRT+
Sbjct: 207 GSSDRTI 213
>XP_006721184
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GASDRTM 7
           G+SDRT+
Sbjct: 207 GSSDRTI 213
>XP_005269639
          Length = 1580

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    GASDRTMSG 9
            G  DR++SG
Sbjct: 1092 GFKDRSLSG 1100
>XP_006717716
          Length = 1619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    GASDRTMSG 9
            G  DR++SG
Sbjct: 1131 GFKDRSLSG 1139
>XP_006717715
          Length = 1660

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    GASDRTMSG 9
            G  DR++SG
Sbjct: 1125 GFKDRSLSG 1133
>XP_006717714
          Length = 1665

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    GASDRTMSG 9
            G  DR++SG
Sbjct: 1182 GFKDRSLSG 1190
>XP_006717713
          Length = 1666

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    GASDRTMSG 9
            G  DR++SG
Sbjct: 1131 GFKDRSLSG 1139
>XP_006717712
          Length = 1670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    GASDRTMSG 9
            G  DR++SG
Sbjct: 1182 GFKDRSLSG 1190
>XP_006717711
          Length = 1677

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    GASDRTMSG 9
            G  DR++SG
Sbjct: 1142 GFKDRSLSG 1150
>XP_005269635
          Length = 1695

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    GASDRTMSG 9
            G  DR++SG
Sbjct: 1160 GFKDRSLSG 1168
>XP_006717710
          Length = 1706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    GASDRTMSG 9
            G  DR++SG
Sbjct: 1171 GFKDRSLSG 1179
>XP_006717709
          Length = 1712

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    GASDRTMSG 9
            G  DR++SG
Sbjct: 1182 GFKDRSLSG 1190
>XP_006717708
          Length = 1717

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    GASDRTMSG 9
            G  DR++SG
Sbjct: 1182 GFKDRSLSG 1190
>XP_006710700
          Length = 1479

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GASDRTMSG 9
           G+SD  +SG
Sbjct: 487 GSSDEELSG 495
>XP_006710699
          Length = 1766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GASDRTMSG 9
           G+SD  +SG
Sbjct: 774 GSSDEELSG 782
>XP_006710698
          Length = 1907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    GASDRTMSG 9
            G+SD  +SG
Sbjct: 1065 GSSDEELSG 1073
>XP_005244806
          Length = 2049

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    GASDRTMSG 9
            G+SD  +SG
Sbjct: 1065 GSSDEELSG 1073
>XP_006710697
          Length = 2053

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    GASDRTMSG 9
            G+SD  +SG
Sbjct: 1065 GSSDEELSG 1073
>XP_006710696
          Length = 2057

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    GASDRTMSG 9
            G+SD  +SG
Sbjct: 1065 GSSDEELSG 1073
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.302    0.117    0.313 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 443,991
Number of extensions: 670
Number of successful extensions: 44
Number of sequences better than 10000.0: 44
Number of HSP's gapped: 44
Number of HSP's successfully gapped: 44
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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