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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001155973                                                           19     869
NP_055903                                                              19     869
NP_689737                                                              16    5631
NP_001153518                                                           16    5631
NP_001153505                                                           16    5631
XP_005246402                                                           16    5631
XP_006712393                                                           16    5631
XP_005246401                                                           16    5631
NP_055907                                                              15    9605
XP_005255242                                                           15    9605
XP_005255241                                                           15    9605
XP_005255240                                                           15    9605
XP_005255239                                                           15    9605

>NP_001155973
          Length = 1833

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   GNASLASK 8
           GNASLASK
Sbjct: 787 GNASLASK 794



>NP_055903
          Length = 1723

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   GNASLASK 8
           GNASLASK
Sbjct: 787 GNASLASK 794



>NP_689737
          Length = 1265

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  NASLASKV 9
          N SLASK+
Sbjct: 32 NVSLASKI 39



>NP_001153518
          Length = 1261

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  NASLASKV 9
          N SLASK+
Sbjct: 32 NVSLASKI 39



>NP_001153505
          Length = 1260

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  NASLASKV 9
          N SLASK+
Sbjct: 32 NVSLASKI 39



>XP_005246402
          Length = 1265

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  NASLASKV 9
          N SLASK+
Sbjct: 32 NVSLASKI 39



>XP_006712393
          Length = 1267

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  NASLASKV 9
          N SLASK+
Sbjct: 38 NVSLASKI 45



>XP_005246401
          Length = 1271

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  NASLASKV 9
          N SLASK+
Sbjct: 38 NVSLASKI 45



>NP_055907
          Length = 3661

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GNASLASKV 9
            GN SLA+++
Sbjct: 1436 GNVSLATRL 1444



>XP_005255242
          Length = 3609

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GNASLASKV 9
            GN SLA+++
Sbjct: 1384 GNVSLATRL 1392



>XP_005255241
          Length = 3637

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GNASLASKV 9
            GN SLA+++
Sbjct: 1384 GNVSLATRL 1392



>XP_005255240
          Length = 3663

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GNASLASKV 9
            GN SLA+++
Sbjct: 1410 GNVSLATRL 1418



>XP_005255239
          Length = 3689

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    GNASLASKV 9
            GN SLA+++
Sbjct: 1436 GNVSLATRL 1444



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.295    0.110    0.259 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 271,120
Number of extensions: 295
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)