Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005912                                                              20     509
XP_005248577                                                           20     509
XP_005248576                                                           20     509
NP_004781                                                              17    2528
NP_695008                                                              17    2528
NP_695010                                                              17    2528
NP_695009                                                              17    2528
XP_006723521                                                           17    4312
NP_001010851                                                           17    4312
NP_853556                                                              16    5631
NP_001193730                                                           16    5631
NP_031396                                                              16    5631
NP_001171559                                                           16    7355
NP_003889                                                              16    7355
NP_055445                                                              16    7355
XP_005255053                                                           16    7355
XP_005268209                                                           16    7355
XP_005268208                                                           16    7355
XP_005267256                                                           16    7355
XP_005267255                                                           16    7355
XP_006715655                                                           16    7355
XP_005267254                                                           16    7355
XP_006715654                                                           16    7355
XP_003403793                                                           16    7355
NP_003805                                                              16    7355
NP_001360                                                              15    9605
NP_003257                                                              15    9605
NP_612564                                                              15    9605
NP_001230526                                                           15    9605
NP_065969                                                              15    9605
NP_001231878                                                           15    9605
NP_058648                                                              15    9605
NP_065136                                                              15    9605
NP_787954                                                              15    9605
NP_059130                                                              15    9605
NP_982354                                                              15    9605
NP_060549                                                              15    9605
NP_665810                                                              15    9605
XP_005254520                                                           15    9605
XP_005266465                                                           15    9605
XP_005266464                                                           15    9605
XP_005266463                                                           15    9605
XP_005270056                                                           15    9605
XP_005270055                                                           15    9605
XP_005248319                                                           15    9605
XP_005264455                                                           15    9605
NP_001172078                                                           15    9605
NP_001104789                                                           15    9605
>NP_005912
          Length = 1512

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   NTIQKFVS 9
           NTIQKFVS
Sbjct: 406 NTIQKFVS 413
>XP_005248577
          Length = 1349

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   NTIQKFVS 9
           NTIQKFVS
Sbjct: 243 NTIQKFVS 250
>XP_005248576
          Length = 1375

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   NTIQKFVS 9
           NTIQKFVS
Sbjct: 269 NTIQKFVS 276
>NP_004781
          Length = 563

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  HNTIQKFVS 9
          HNT+Q F +
Sbjct: 34 HNTLQNFTA 42
>NP_695008
          Length = 550

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  HNTIQKFVS 9
          HNT+Q F +
Sbjct: 34 HNTLQNFTA 42
>NP_695010
          Length = 519

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  HNTIQKFVS 9
          HNT+Q F +
Sbjct: 34 HNTLQNFTA 42
>NP_695009
          Length = 506

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  HNTIQKFVS 9
          HNT+Q F +
Sbjct: 34 HNTLQNFTA 42
>XP_006723521
          Length = 425

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  NTIQKFVS 9
          N +QKF+S
Sbjct: 91 NQVQKFIS 98
>NP_001010851
          Length = 468

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NTIQKFVS 9
           N +QKF+S
Sbjct: 134 NQVQKFIS 141
>NP_853556
          Length = 319

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  NTIQKFVS 9
          +TIQKF++
Sbjct: 21 STIQKFIN 28
>NP_001193730
          Length = 337

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  NTIQKFVS 9
          +TIQKF++
Sbjct: 42 STIQKFIN 49
>NP_031396
          Length = 340

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  NTIQKFVS 9
          +TIQKF++
Sbjct: 42 STIQKFIN 49
>NP_001171559
          Length = 1259

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   NTIQKFVS 9
           NT+Q F++
Sbjct: 157 NTVQSFIA 164
>NP_003889
          Length = 1496

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   NTIQKFVS 9
           NT+Q F++
Sbjct: 394 NTVQSFIA 401
>NP_055445
          Length = 1267

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    HNTIQKF 7
            H+T++KF
Sbjct: 1036 HDTVKKF 1042
>XP_005255053
          Length = 1267

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    HNTIQKF 7
            H+T++KF
Sbjct: 1036 HDTVKKF 1042
>XP_005268209
          Length = 776

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   HNTIQKF 7
           H+T+Q+F
Sbjct: 628 HSTVQQF 634
>XP_005268208
          Length = 776

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   HNTIQKF 7
           H+T+Q+F
Sbjct: 628 HSTVQQF 634
>XP_005267256
          Length = 1113

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  NTIQKFVS 9
          NT+Q F++
Sbjct: 11 NTVQSFIA 18
>XP_005267255
          Length = 1165

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  NTIQKFVS 9
          NT+Q F++
Sbjct: 63 NTVQSFIA 70
>XP_006715655
          Length = 1288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   NTIQKFVS 9
           NT+Q F++
Sbjct: 394 NTVQSFIA 401
>XP_005267254
          Length = 1443

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   NTIQKFVS 9
           NT+Q F++
Sbjct: 341 NTVQSFIA 348
>XP_006715654
          Length = 1495

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   NTIQKFVS 9
           NT+Q F++
Sbjct: 393 NTVQSFIA 400
>XP_003403793
          Length = 846

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   HNTIQKFV 8
           +NT+QK++
Sbjct: 450 NNTVQKYL 457
>NP_003805
          Length = 776

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   HNTIQKF 7
           H+T+Q+F
Sbjct: 628 HSTVQQF 634
>NP_001360
          Length = 4624

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HNTIQKFV 8
           HN ++KF+
Sbjct: 548 HNELRKFM 555
>NP_003257
          Length = 799

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1   HNTIQKF 7
           HN IQ F
Sbjct: 119 HNLIQSF 125
>NP_612564
          Length = 839

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1   HNTIQKF 7
           HN IQ F
Sbjct: 159 HNLIQSF 165
>NP_001230526
          Length = 1287

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    NTIQKF 7
            NTIQK+
Sbjct: 995  NTIQKY 1000
>NP_065969
          Length = 828

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1   HNTIQKFV 8
           H T+ KF+
Sbjct: 644 HETVGKFI 651
>NP_001231878
          Length = 828

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1   HNTIQKFV 8
           H T+ KF+
Sbjct: 644 HETVGKFI 651
>NP_058648
          Length = 1264

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    NTIQKF 7
            NTIQK+
Sbjct: 995  NTIQKY 1000
>NP_065136
          Length = 1203

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   HNTIQKFVS 9
           HN  Q FVS
Sbjct: 574 HNHFQFFVS 582
>NP_787954
          Length = 1551

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1    HNTIQKF 7
            H T+Q+F
Sbjct: 1028 HQTVQQF 1034
>NP_059130
          Length = 1551

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1    HNTIQKF 7
            H T+Q+F
Sbjct: 1028 HQTVQQF 1034
>NP_982354
          Length = 1237

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   HNTIQKFVS 9
           HN  Q FVS
Sbjct: 574 HNHFQFFVS 582
>NP_060549
          Length = 995

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HNTIQK 6
           H+TIQK
Sbjct: 279 HSTIQK 284
>NP_665810
          Length = 275

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   HNTIQKFVS 9
           H TI  FVS
Sbjct: 126 HRTILAFVS 134
>XP_005254520
          Length = 1235

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   HNTIQKF 7
           H T+Q+F
Sbjct: 712 HQTVQQF 718
>XP_005266465
          Length = 1019

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   HNTIQKFVS 9
           HN  Q FVS
Sbjct: 574 HNHFQFFVS 582
>XP_005266464
          Length = 1161

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   HNTIQKFVS 9
           HN  Q FVS
Sbjct: 574 HNHFQFFVS 582
>XP_005266463
          Length = 1195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   HNTIQKFVS 9
           HN  Q FVS
Sbjct: 574 HNHFQFFVS 582
>XP_005270056
          Length = 789

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1   HNTIQKFV 8
           H T+ KF+
Sbjct: 644 HETVGKFI 651
>XP_005270055
          Length = 828

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1   HNTIQKFV 8
           H T+ KF+
Sbjct: 644 HETVGKFI 651
>XP_005248319
          Length = 4609

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   HNTIQKFV 8
           HN ++KF+
Sbjct: 533 HNELRKFM 540
>XP_005264455
          Length = 671

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HNTIQK 6
           H+TIQK
Sbjct: 279 HSTIQK 284
>NP_001172078
          Length = 220

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   HNTIQKF 7
           H T+Q+F
Sbjct: 142 HKTVQEF 148
>NP_001104789
          Length = 220

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   HNTIQKF 7
           H T+Q+F
Sbjct: 142 HKTVQEF 148
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.127    0.361 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 387,125
Number of extensions: 605
Number of successful extensions: 48
Number of sequences better than 10000.0: 48
Number of HSP's gapped: 48
Number of HSP's successfully gapped: 48
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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