Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002040                                                              18    1935
NP_002696                                                              17    4312
XP_006720965                                                           17    4312
NP_001103691                                                           16    7355
NP_001103690                                                           16    7355
NP_006774                                                              16    7355
NP_001103689                                                           16    7355
NP_705895                                                              15    9605
NP_002137                                                              15    9605
XP_005257339                                                           15    9605
XP_005257337                                                           15    9605
XP_006721918                                                           15    9605
XP_005257334                                                           15    9605
XP_006721917                                                           15    9605
XP_005249789                                                           15    9605
XP_005249788                                                           15    9605
XP_006715778                                                           15    9605
XP_005249787                                                           15    9605
NP_004875                                                              15    9605
NP_109377                                                              15    9605
>NP_002040
          Length = 413

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IVSKRARTQ 9
           IVSKRA TQ
Sbjct: 249 IVSKRAGTQ 257
>NP_002696
          Length = 1756

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   VSKRARTQ 9
           VSKRAR Q
Sbjct: 822 VSKRARLQ 829
>XP_006720965
          Length = 1769

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   VSKRARTQ 9
           VSKRAR Q
Sbjct: 835 VSKRARLQ 842
>NP_001103691
          Length = 261

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SKRARTQ 9
           SKRA+TQ
Sbjct: 222 SKRAQTQ 228
>NP_001103690
          Length = 261

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SKRARTQ 9
           SKRA+TQ
Sbjct: 222 SKRAQTQ 228
>NP_006774
          Length = 261

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SKRARTQ 9
           SKRA+TQ
Sbjct: 222 SKRAQTQ 228
>NP_001103689
          Length = 261

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SKRARTQ 9
           SKRA+TQ
Sbjct: 222 SKRAQTQ 228
>NP_705895
          Length = 443

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SKRART 8
           SKRART
Sbjct: 191 SKRART 196
>NP_002137
          Length = 431

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SKRART 8
           SKRART
Sbjct: 188 SKRART 193
>XP_005257339
          Length = 299

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  SKRART 8
          SKRART
Sbjct: 56 SKRART 61
>XP_005257337
          Length = 358

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SKRART 8
           SKRART
Sbjct: 115 SKRART 120
>XP_006721918
          Length = 431

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SKRART 8
           SKRART
Sbjct: 188 SKRART 193
>XP_005257334
          Length = 431

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SKRART 8
           SKRART
Sbjct: 188 SKRART 193
>XP_006721917
          Length = 431

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SKRART 8
           SKRART
Sbjct: 188 SKRART 193
>XP_005249789
          Length = 443

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SKRART 8
           SKRART
Sbjct: 191 SKRART 196
>XP_005249788
          Length = 443

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SKRART 8
           SKRART
Sbjct: 191 SKRART 196
>XP_006715778
          Length = 443

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SKRART 8
           SKRART
Sbjct: 191 SKRART 196
>XP_005249787
          Length = 443

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SKRART 8
           SKRART
Sbjct: 191 SKRART 196
>NP_004875
          Length = 814

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IVSKRARTQ 9
           +VS++AR Q
Sbjct: 126 VVSRKARIQ 134
>NP_109377
          Length = 443

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SKRART 8
           SKRART
Sbjct: 191 SKRART 196
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.122    0.287 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 286,268
Number of extensions: 345
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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