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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              17    2528
XP_006716142                                                           17    2528
XP_005250088                                                           17    2528
XP_006716141                                                           17    2528
XP_005250085                                                           17    2528
XP_006716140                                                           17    2528
XP_005250084                                                           17    2528
XP_005250083                                                           17    2528
XP_005250082                                                           17    2528
NP_055817                                                              17    3301
NP_296373                                                              17    3301
XP_005263028                                                           17    3301
NP_061837                                                              16    7355
NP_001094299                                                           16    7355
XP_005261198                                                           16    7355
XP_006724079                                                           16    7355
XP_006724078                                                           16    7355
XP_006724077                                                           16    7355
XP_005261197                                                           16    7355
XP_005261196                                                           16    7355

>NP_733751
          Length = 4911

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KIIISFSRR 9
            KIIIS SRR
Sbjct: 4867 KIIISSSRR 4875



>XP_006716142
          Length = 4927

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KIIISFSRR 9
            KIIIS SRR
Sbjct: 4883 KIIISSSRR 4891



>XP_005250088
          Length = 4928

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KIIISFSRR 9
            KIIIS SRR
Sbjct: 4884 KIIISSSRR 4892



>XP_006716141
          Length = 4959

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KIIISFSRR 9
            KIIIS SRR
Sbjct: 4915 KIIISSSRR 4923



>XP_005250085
          Length = 4979

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KIIISFSRR 9
            KIIIS SRR
Sbjct: 4935 KIIISSSRR 4943



>XP_006716140
          Length = 4982

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KIIISFSRR 9
            KIIIS SRR
Sbjct: 4938 KIIISSSRR 4946



>XP_005250084
          Length = 4982

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KIIISFSRR 9
            KIIIS SRR
Sbjct: 4938 KIIISSSRR 4946



>XP_005250083
          Length = 4982

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KIIISFSRR 9
            KIIIS SRR
Sbjct: 4938 KIIISSSRR 4946



>XP_005250082
          Length = 4983

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KIIISFSRR 9
            KIIIS SRR
Sbjct: 4939 KIIISSSRR 4947



>NP_055817
          Length = 621

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KIIISFSRR 9
           KI+ SFS+R
Sbjct: 112 KIVASFSKR 120



>NP_296373
          Length = 628

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KIIISFSRR 9
           KI+ SFS+R
Sbjct: 112 KIVASFSKR 120



>XP_005263028
          Length = 152

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   IIISFSRR 9
           ++ISFSRR
Sbjct: 128 LLISFSRR 135



>NP_061837
          Length = 533

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  KIIISFSR 8
          + IISFSR
Sbjct: 9  RFIISFSR 16



>NP_001094299
          Length = 132

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   IIISFSR 8
           +IISFSR
Sbjct: 121 LIISFSR 127



>XP_005261198
          Length = 533

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  KIIISFSR 8
          + IISFSR
Sbjct: 9  RFIISFSR 16



>XP_006724079
          Length = 562

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  KIIISFSR 8
          + IISFSR
Sbjct: 9  RFIISFSR 16



>XP_006724078
          Length = 562

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  KIIISFSR 8
          + IISFSR
Sbjct: 9  RFIISFSR 16



>XP_006724077
          Length = 562

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  KIIISFSR 8
          + IISFSR
Sbjct: 9  RFIISFSR 16



>XP_005261197
          Length = 562

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  KIIISFSR 8
          + IISFSR
Sbjct: 9  RFIISFSR 16



>XP_005261196
          Length = 562

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  KIIISFSR 8
          + IISFSR
Sbjct: 9  RFIISFSR 16



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.345    0.152    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 356,141
Number of extensions: 448
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)