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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001136113                                                           18    1935
NP_000537                                                              17    4312
NP_001263689                                                           17    4312
NP_001119584                                                           17    4312
NP_001119586                                                           17    4312
NP_001263625                                                           17    4312
NP_001119590                                                           17    4312
NP_001263690                                                           17    4312
NP_001119585                                                           17    4312
NP_001263624                                                           17    4312
NP_001157666                                                           16    7355
NP_001165124                                                           16    7355
NP_055610                                                              16    7355
NP_001157661                                                           16    7355
NP_005808                                                              16    7355
NP_036559                                                              16    7355
NP_001070960                                                           16    7355
NP_001070959                                                           16    7355
XP_006712945                                                           16    7355
XP_006712944                                                           16    7355
NP_733751                                                              15    9605
NP_005388                                                              15    9605
XP_006716142                                                           15    9605
XP_005250088                                                           15    9605
XP_006716141                                                           15    9605
XP_005250085                                                           15    9605
XP_006716140                                                           15    9605
XP_005250084                                                           15    9605
XP_005250083                                                           15    9605
XP_005250082                                                           15    9605

>NP_001136113
          Length = 1045

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   LSSSVLSQK 9
           LS+S+LSQK
Sbjct: 579 LSASILSQK 587



>NP_000537
          Length = 393

 Score = 16.5 bits (31), Expect =  4312
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LSSSVLSQK 9
           LSSSV SQK
Sbjct: 93  LSSSVPSQK 101



>NP_001263689
          Length = 354

 Score = 16.5 bits (31), Expect =  4312
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  LSSSVLSQK 9
          LSSSV SQK
Sbjct: 54 LSSSVPSQK 62



>NP_001119584
          Length = 393

 Score = 16.5 bits (31), Expect =  4312
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LSSSVLSQK 9
           LSSSV SQK
Sbjct: 93  LSSSVPSQK 101



>NP_001119586
          Length = 341

 Score = 16.5 bits (31), Expect =  4312
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LSSSVLSQK 9
           LSSSV SQK
Sbjct: 93  LSSSVPSQK 101



>NP_001263625
          Length = 302

 Score = 16.5 bits (31), Expect =  4312
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  LSSSVLSQK 9
          LSSSV SQK
Sbjct: 54 LSSSVPSQK 62



>NP_001119590
          Length = 354

 Score = 16.5 bits (31), Expect =  4312
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  LSSSVLSQK 9
          LSSSV SQK
Sbjct: 54 LSSSVPSQK 62



>NP_001263690
          Length = 354

 Score = 16.5 bits (31), Expect =  4312
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  LSSSVLSQK 9
          LSSSV SQK
Sbjct: 54 LSSSVPSQK 62



>NP_001119585
          Length = 346

 Score = 16.5 bits (31), Expect =  4312
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LSSSVLSQK 9
           LSSSV SQK
Sbjct: 93  LSSSVPSQK 101



>NP_001263624
          Length = 307

 Score = 16.5 bits (31), Expect =  4312
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  LSSSVLSQK 9
          LSSSV SQK
Sbjct: 54 LSSSVPSQK 62



>NP_001157666
          Length = 422

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LSSSVLSQ 8
           LSSS+L+Q
Sbjct: 370 LSSSILAQ 377



>NP_001165124
          Length = 1190

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LSSSVLSQK 9
           L SSVLS+K
Sbjct: 611 LLSSVLSEK 619



>NP_055610
          Length = 1214

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LSSSVLSQK 9
           L SSVLS+K
Sbjct: 635 LLSSVLSEK 643



>NP_001157661
          Length = 433

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LSSSVLSQ 8
           LSSS+L+Q
Sbjct: 381 LSSSILAQ 388



>NP_005808
          Length = 434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LSSSVLSQ 8
           LSSS+L+Q
Sbjct: 382 LSSSILAQ 389



>NP_036559
          Length = 293

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LSSSVLSQK 9
           L+ SVLSQK
Sbjct: 207 LNISVLSQK 215



>NP_001070960
          Length = 293

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LSSSVLSQK 9
           L+ SVLSQK
Sbjct: 207 LNISVLSQK 215



>NP_001070959
          Length = 293

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LSSSVLSQK 9
           L+ SVLSQK
Sbjct: 207 LNISVLSQK 215



>XP_006712945
          Length = 1214

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LSSSVLSQK 9
           L SSVLS+K
Sbjct: 635 LLSSVLSEK 643



>XP_006712944
          Length = 1214

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LSSSVLSQK 9
           L SSVLS+K
Sbjct: 635 LLSSVLSEK 643



>NP_733751
          Length = 4911

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    SSSVLSQK 9
            SSS+L+QK
Sbjct: 3786 SSSLLNQK 3793



>NP_005388
          Length = 526

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSSSVLSQK 9
           L SSV+SQ+
Sbjct: 235 LPSSVISQR 243



>XP_006716142
          Length = 4927

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    SSSVLSQK 9
            SSS+L+QK
Sbjct: 3803 SSSLLNQK 3810



>XP_005250088
          Length = 4928

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    SSSVLSQK 9
            SSS+L+QK
Sbjct: 3803 SSSLLNQK 3810



>XP_006716141
          Length = 4959

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    SSSVLSQK 9
            SSS+L+QK
Sbjct: 3803 SSSLLNQK 3810



>XP_005250085
          Length = 4979

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    SSSVLSQK 9
            SSS+L+QK
Sbjct: 3803 SSSLLNQK 3810



>XP_006716140
          Length = 4982

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    SSSVLSQK 9
            SSS+L+QK
Sbjct: 3803 SSSLLNQK 3810



>XP_005250084
          Length = 4982

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    SSSVLSQK 9
            SSS+L+QK
Sbjct: 3803 SSSLLNQK 3810



>XP_005250083
          Length = 4982

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    SSSVLSQK 9
            SSS+L+QK
Sbjct: 3802 SSSLLNQK 3809



>XP_005250082
          Length = 4983

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    SSSVLSQK 9
            SSS+L+QK
Sbjct: 3803 SSSLLNQK 3810



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.289    0.102    0.206 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 320,566
Number of extensions: 475
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)