Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              18    1135
NP_001035957                                                           18    1135
XP_006721991                                                           18    1135
XP_006721990                                                           18    1135
XP_006721989                                                           18    1135
XP_005258041                                                           18    1135
XP_006721988                                                           18    1135
XP_006721987                                                           18    1135
XP_006721986                                                           18    1135
XP_005258040                                                           18    1135
XP_006721985                                                           18    1135
NP_001253969                                                           16    7355
NP_004964                                                              16    7355
XP_005249146                                                           16    7355
NP_060469                                                              16    7355
NP_056099                                                              15    9605
NP_001159711                                                           15    9605
NP_006560                                                              15    9605
NP_001159712                                                           15    9605
XP_006710565                                                           15    9605
XP_006710564                                                           15    9605
XP_005270743                                                           15    9605
NP_789791                                                              15    9605
>NP_000258
          Length = 2818

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RGLKLMSMI 9
            RGLKLMS I
Sbjct: 1416 RGLKLMSKI 1424
>NP_001035957
          Length = 2839

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RGLKLMSMI 9
            RGLKLMS I
Sbjct: 1437 RGLKLMSKI 1445
>XP_006721991
          Length = 1632

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RGLKLMSMI 9
            RGLKLMS I
Sbjct: 1447 RGLKLMSKI 1455
>XP_006721990
          Length = 2823

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RGLKLMSMI 9
            RGLKLMS I
Sbjct: 1447 RGLKLMSKI 1455
>XP_006721989
          Length = 2823

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RGLKLMSMI 9
            RGLKLMS I
Sbjct: 1447 RGLKLMSKI 1455
>XP_005258041
          Length = 2836

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RGLKLMSMI 9
            RGLKLMS I
Sbjct: 1416 RGLKLMSKI 1424
>XP_006721988
          Length = 2846

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RGLKLMSMI 9
            RGLKLMS I
Sbjct: 1426 RGLKLMSKI 1434
>XP_006721987
          Length = 2849

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RGLKLMSMI 9
            RGLKLMS I
Sbjct: 1447 RGLKLMSKI 1455
>XP_006721986
          Length = 2854

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RGLKLMSMI 9
            RGLKLMS I
Sbjct: 1434 RGLKLMSKI 1442
>XP_005258040
          Length = 2857

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RGLKLMSMI 9
            RGLKLMS I
Sbjct: 1437 RGLKLMSKI 1445
>XP_006721985
          Length = 2867

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RGLKLMSMI 9
            RGLKLMS I
Sbjct: 1447 RGLKLMSKI 1455
>NP_001253969
          Length = 1074

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    RGLKLM 6
            RGLKLM
Sbjct: 1004 RGLKLM 1009
>NP_004964
          Length = 1246

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    RGLKLM 6
            RGLKLM
Sbjct: 1176 RGLKLM 1181
>XP_005249146
          Length = 1074

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    RGLKLM 6
            RGLKLM
Sbjct: 1004 RGLKLM 1009
>NP_060469
          Length = 1072

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   GLKLMSMI 9
           GL+L+SMI
Sbjct: 488 GLQLVSMI 495
>NP_056099
          Length = 3375

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    RGLKLMS 7
            RGL LMS
Sbjct: 1794 RGLSLMS 1800
>NP_001159711
          Length = 318

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  GLKLMSMI 9
          GL+L+SM+
Sbjct: 91 GLELLSML 98
>NP_006560
          Length = 318

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  GLKLMSMI 9
          GL+L+SM+
Sbjct: 91 GLELLSML 98
>NP_001159712
          Length = 132

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  GLKLMSMI 9
          GL+L+SM+
Sbjct: 91 GLELLSML 98
>XP_006710565
          Length = 3140

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    RGLKLMS 7
            RGL LMS
Sbjct: 1559 RGLSLMS 1565
>XP_006710564
          Length = 3432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    RGLKLMS 7
            RGL LMS
Sbjct: 1851 RGLSLMS 1857
>XP_005270743
          Length = 3453

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    RGLKLMS 7
            RGL LMS
Sbjct: 1872 RGLSLMS 1878
>NP_789791
          Length = 655

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  RGLKLMSMI 9
          +GLK+M+++
Sbjct: 76 KGLKVMNLL 84
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.338    0.147    0.378 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 281,877
Number of extensions: 153
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)

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