Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115803                                                              20     390
NP_031376                                                              18    1482
NP_891550                                                              17    3301
XP_005265386                                                           17    3301
XP_006710239                                                           17    3301
NP_005711                                                              17    4312
XP_006715889                                                           17    4312
XP_006715888                                                           17    4312
NP_065863                                                              16    5631
XP_005260057                                                           16    5631
XP_006722867                                                           16    5631
XP_006722866                                                           16    5631
XP_006722865                                                           16    5631
XP_005260058                                                           16    5631
NP_006336                                                              16    5631
NP_783864                                                              16    7355
NP_055947                                                              16    7355
NP_001014980                                                           16    7355
XP_005249729                                                           16    7355
XP_005262701                                                           16    7355
NP_000913                                                              15    9605
XP_006718312                                                           15    9605
XP_005253029                                                           15    9605
XP_005270390                                                           15    9605
XP_006711823                                                           15    9605
NP_733843                                                              15    9605
NP_001017434                                                           15    9605

>NP_115803
          Length = 1156

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RLRTRIAVC 9
          RLR RIAVC
Sbjct: 39 RLRARIAVC 47



>NP_031376
          Length = 401

 Score = 18.1 bits (35), Expect =  1482
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  RLRTRIAVC 9
          R+RTR+++C
Sbjct: 72 RIRTRLSLC 80



>NP_891550
          Length = 1935

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LRTRIAVC 9
            +RTR+ VC
Sbjct: 1402 IRTRLVVC 1409



>XP_005265386
          Length = 1907

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LRTRIAVC 9
            +RTR+ VC
Sbjct: 1374 IRTRLVVC 1381



>XP_006710239
          Length = 485

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LRTRIAVC 9
           LR+R++VC
Sbjct: 339 LRSRVSVC 346



>NP_005711
          Length = 372

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  RTRIAVC 9
          RT+IA+C
Sbjct: 20 RTQIAIC 26



>XP_006715889
          Length = 372

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  RTRIAVC 9
          RT+IA+C
Sbjct: 20 RTQIAIC 26



>XP_006715888
          Length = 372

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  RTRIAVC 9
          RT+IA+C
Sbjct: 20 RTQIAIC 26



>NP_065863
          Length = 2047

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LRTRIAVC 9
            ++TRI VC
Sbjct: 1885 IKTRIRVC 1892



>XP_005260057
          Length = 2113

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LRTRIAVC 9
            ++TRI VC
Sbjct: 1951 IKTRIRVC 1958



>XP_006722867
          Length = 1159

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LRTRIAVC 9
            ++TRI VC
Sbjct: 997  IKTRIRVC 1004



>XP_006722866
          Length = 1778

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LRTRIAVC 9
            ++TRI VC
Sbjct: 1616 IKTRIRVC 1623



>XP_006722865
          Length = 2078

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LRTRIAVC 9
            ++TRI VC
Sbjct: 1916 IKTRIRVC 1923



>XP_005260058
          Length = 2082

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LRTRIAVC 9
            ++TRI VC
Sbjct: 1920 IKTRIRVC 1927



>NP_006336
          Length = 568

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  RLRTRIAVC 9
          RLR R A C
Sbjct: 22 RLRCRAAAC 30



>NP_783864
          Length = 1044

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RLRTRIAVC 9
           RL+TR A C
Sbjct: 568 RLKTRPAQC 576



>NP_055947
          Length = 957

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RLRTRIA 7
           R+RTR+A
Sbjct: 859 RMRTRVA 865



>NP_001014980
          Length = 302

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RLRTRIAVC 9
           RLR R  VC
Sbjct: 223 RLRARDVVC 231



>XP_005249729
          Length = 968

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RLRTRIA 7
           R+RTR+A
Sbjct: 870 RMRTRVA 876



>XP_005262701
          Length = 1035

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RLRTRIAVC 9
           RL+TR A C
Sbjct: 559 RLKTRPAQC 567



>NP_000913
          Length = 1112

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LRTRIAVC 9
           LRT ++VC
Sbjct: 108 LRTLLSVC 115



>XP_006718312
          Length = 735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LRTRIAVC 9
           LRT ++VC
Sbjct: 108 LRTLLSVC 115



>XP_005253029
          Length = 1029

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LRTRIAVC 9
           LRT ++VC
Sbjct: 108 LRTLLSVC 115



>XP_005270390
          Length = 589

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RTRIAVC 9
           RTR A+C
Sbjct: 557 RTRCALC 563



>XP_006711823
          Length = 592

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RTRIAVC 9
           RTR A+C
Sbjct: 560 RTRCALC 566



>NP_733843
          Length = 592

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RTRIAVC 9
           RTR A+C
Sbjct: 560 RTRCALC 566



>NP_001017434
          Length = 341

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RTRIAVC 9
           RTR A+C
Sbjct: 309 RTRCALC 315



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.352    0.147    0.475 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 396,703
Number of extensions: 708
Number of successful extensions: 27
Number of sequences better than 10000.0: 27
Number of HSP's gapped: 27
Number of HSP's successfully gapped: 27
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.0 bits)
S2: 28 (15.4 bits)