Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_036462                                                              18    1482
NP_001243397                                                           18    1482
XP_005269721                                                           18    1482
NP_009225                                                              17    4312
NP_009230                                                              17    4312
NP_009228                                                              17    4312
NP_009229                                                              17    4312
NP_001931                                                              17    4312
NP_001007027                                                           17    4312
NP_009231                                                              17    4312
NP_005550                                                              17    4312
XP_006722104                                                           17    4312
XP_006722103                                                           17    4312
XP_006722102                                                           17    4312
XP_006722101                                                           17    4312
XP_006722100                                                           17    4312
XP_006722099                                                           17    4312
XP_006722098                                                           17    4312
XP_006722097                                                           17    4312
XP_006722096                                                           17    4312
XP_006722095                                                           17    4312
XP_006722094                                                           17    4312
XP_006722093                                                           17    4312
XP_006722092                                                           17    4312
NP_001010870                                                           16    5631
NP_001161831                                                           16    5631
NP_000483                                                              16    7355
XP_006715905                                                           16    7355
NP_001127951                                                           15    9605
NP_060573                                                              15    9605
XP_005271048                                                           15    9605

>NP_036462
          Length = 2073

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SASTRKVNK 9
           SAST KVNK
Sbjct: 421 SASTLKVNK 429



>NP_001243397
          Length = 1890

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SASTRKVNK 9
           SAST KVNK
Sbjct: 421 SASTLKVNK 429



>XP_005269721
          Length = 2073

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SASTRKVNK 9
           SAST KVNK
Sbjct: 421 SASTLKVNK 429



>NP_009225
          Length = 1863

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    SASTRKVNK 9
            +AST +VNK
Sbjct: 1640 TASTERVNK 1648



>NP_009230
          Length = 699

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SASTRKVNK 9
           +AST +VNK
Sbjct: 536 TASTERVNK 544



>NP_009228
          Length = 1816

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    SASTRKVNK 9
            +AST +VNK
Sbjct: 1593 TASTERVNK 1601



>NP_009229
          Length = 759

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SASTRKVNK 9
           +AST +VNK
Sbjct: 536 TASTERVNK 544



>NP_001931
          Length = 1190

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  ASTRKVNK 9
          AST KVNK
Sbjct: 62 ASTPKVNK 69



>NP_001007027
          Length = 1190

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  ASTRKVNK 9
          AST KVNK
Sbjct: 62 ASTPKVNK 69



>NP_009231
          Length = 1884

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    SASTRKVNK 9
            +AST +VNK
Sbjct: 1661 TASTERVNK 1669



>NP_005550
          Length = 3075

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    ASTRKVNK 9
            AST+KVN+
Sbjct: 1638 ASTQKVNR 1645



>XP_006722104
          Length = 718

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SASTRKVNK 9
           +AST +VNK
Sbjct: 495 TASTERVNK 503



>XP_006722103
          Length = 759

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SASTRKVNK 9
           +AST +VNK
Sbjct: 536 TASTERVNK 544



>XP_006722102
          Length = 759

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SASTRKVNK 9
           +AST +VNK
Sbjct: 536 TASTERVNK 544



>XP_006722101
          Length = 759

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SASTRKVNK 9
           +AST +VNK
Sbjct: 536 TASTERVNK 544



>XP_006722100
          Length = 760

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SASTRKVNK 9
           +AST +VNK
Sbjct: 537 TASTERVNK 545



>XP_006722099
          Length = 1736

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    SASTRKVNK 9
            +AST +VNK
Sbjct: 1513 TASTERVNK 1521



>XP_006722098
          Length = 1793

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    SASTRKVNK 9
            +AST +VNK
Sbjct: 1570 TASTERVNK 1578



>XP_006722097
          Length = 1862

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    SASTRKVNK 9
            +AST +VNK
Sbjct: 1639 TASTERVNK 1647



>XP_006722096
          Length = 1862

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    SASTRKVNK 9
            +AST +VNK
Sbjct: 1639 TASTERVNK 1647



>XP_006722095
          Length = 1862

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    SASTRKVNK 9
            +AST +VNK
Sbjct: 1639 TASTERVNK 1647



>XP_006722094
          Length = 1863

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    SASTRKVNK 9
            +AST +VNK
Sbjct: 1640 TASTERVNK 1648



>XP_006722093
          Length = 1863

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    SASTRKVNK 9
            +AST +VNK
Sbjct: 1640 TASTERVNK 1648



>XP_006722092
          Length = 1863

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    SASTRKVNK 9
            +AST +VNK
Sbjct: 1640 TASTERVNK 1648



>NP_001010870
          Length = 2096

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    SASTRKVNK 9
            SAS++ VNK
Sbjct: 1494 SASSKSVNK 1502



>NP_001161831
          Length = 2066

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    SASTRKVNK 9
            SAS++ VNK
Sbjct: 1494 SASSKSVNK 1502



>NP_000483
          Length = 1480

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   SASTRKVN 8
           +ASTRKV+
Sbjct: 788 TASTRKVS 795



>XP_006715905
          Length = 1588

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   SASTRKVN 8
           +ASTRKV+
Sbjct: 896 TASTRKVS 903



>NP_001127951
          Length = 858

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SASTRKVNK 9
           SAST  +NK
Sbjct: 248 SASTPMINK 256



>NP_060573
          Length = 858

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SASTRKVNK 9
           SAST  +NK
Sbjct: 248 SASTPMINK 256



>XP_005271048
          Length = 858

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SASTRKVNK 9
           SAST  +NK
Sbjct: 248 SASTPMINK 256



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.290   0.0985    0.222 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 300,012
Number of extensions: 271
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)