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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              17    2528
NP_001075109                                                           17    2528
NP_055529                                                              17    3301
XP_006721055                                                           17    3301
XP_006721054                                                           17    3301
XP_006721053                                                           17    3301
XP_006721052                                                           17    3301
NP_653165                                                              17    4312
NP_001093321                                                           16    5631
NP_001038945                                                           16    5631
NP_001034450                                                           16    5631
NP_001278310                                                           16    5631
NP_001013712                                                           16    7355
XP_006721609                                                           16    7355
XP_005268927                                                           16    7355
XP_005246247                                                           16    7355
NP_036525                                                              16    7355

>NP_008835
          Length = 4128

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SRLLELAFA 9
            S LLELAFA
Sbjct: 1512 SGLLELAFA 1520



>NP_001075109
          Length = 4097

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SRLLELAFA 9
            S LLELAFA
Sbjct: 1512 SGLLELAFA 1520



>NP_055529
          Length = 1462

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    SRLLELAFA 9
            S+ LELAFA
Sbjct: 1095 SKALELAFA 1103



>XP_006721055
          Length = 673

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SRLLELAFA 9
           S+ LELAFA
Sbjct: 306 SKALELAFA 314



>XP_006721054
          Length = 1462

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    SRLLELAFA 9
            S+ LELAFA
Sbjct: 1095 SKALELAFA 1103



>XP_006721053
          Length = 1462

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    SRLLELAFA 9
            S+ LELAFA
Sbjct: 1095 SKALELAFA 1103



>XP_006721052
          Length = 1462

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    SRLLELAFA 9
            S+ LELAFA
Sbjct: 1095 SKALELAFA 1103



>NP_653165
          Length = 314

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   RLLELAFA 9
           RLL LAFA
Sbjct: 169 RLLSLAFA 176



>NP_001093321
          Length = 474

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1  SRLLELA 7
          SRLLELA
Sbjct: 7  SRLLELA 13



>NP_001038945
          Length = 474

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1  SRLLELA 7
          SRLLELA
Sbjct: 7  SRLLELA 13



>NP_001034450
          Length = 474

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1  SRLLELA 7
          SRLLELA
Sbjct: 7  SRLLELA 13



>NP_001278310
          Length = 474

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1  SRLLELA 7
          SRLLELA
Sbjct: 7  SRLLELA 13



>NP_001013712
          Length = 653

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SRLLELAFA 9
           +RLL+ AFA
Sbjct: 470 ARLLQAAFA 478



>XP_006721609
          Length = 947

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LLELAFA 9
           LLE+AFA
Sbjct: 919 LLEMAFA 925



>XP_005268927
          Length = 653

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SRLLELAFA 9
           +RLL+ AFA
Sbjct: 470 ARLLQAAFA 478



>XP_005246247
          Length = 1417

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    RLLELAF 8
            RLL+LAF
Sbjct: 1388 RLLDLAF 1394



>NP_036525
          Length = 1338

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LLELAFA 9
           LLE+AFA
Sbjct: 919 LLEMAFA 925



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.335    0.141    0.348 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 335,867
Number of extensions: 377
Number of successful extensions: 17
Number of sequences better than 10000.0: 17
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 17
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)