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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002430                                                              20     390
NP_001271427                                                           16    5631
NP_689559                                                              16    5631
NP_001271428                                                           16    5631
XP_006721509                                                           16    5631
XP_006721508                                                           16    5631
XP_006721507                                                           16    5631
NP_001004725                                                           16    7355
NP_001268421                                                           15    9605
NP_000170                                                              15    9605
NP_001268422                                                           15    9605
NP_001268423                                                           15    9605
NP_001275896                                                           15    9605
NP_001275895                                                           15    9605
NP_001275894                                                           15    9605
NP_110409                                                              15    9605
NP_077817                                                              15    9605
NP_003339                                                              15    9605
NP_001275893                                                           15    9605
NP_001273327                                                           15    9605
XP_006715413                                                           15    9605
XP_006714565                                                           15    9605
XP_005247869                                                           15    9605
XP_006713830                                                           15    9605
XP_005247867                                                           15    9605
XP_005247866                                                           15    9605
XP_005247863                                                           15    9605
XP_005247862                                                           15    9605
XP_005247861                                                           15    9605
XP_005247860                                                           15    9605
XP_005247859                                                           15    9605
XP_005264328                                                           15    9605

>NP_002430
          Length = 1137

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TRMGAANNI 9
           TRMGAA+NI
Sbjct: 937 TRMGAADNI 945



>NP_001271427
          Length = 573

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   MGAANNI 9
           MGA NNI
Sbjct: 342 MGAMNNI 348



>NP_689559
          Length = 569

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   MGAANNI 9
           MGA NNI
Sbjct: 342 MGAMNNI 348



>NP_001271428
          Length = 432

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   MGAANNI 9
           MGA NNI
Sbjct: 205 MGAMNNI 211



>XP_006721509
          Length = 536

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   MGAANNI 9
           MGA NNI
Sbjct: 309 MGAMNNI 315



>XP_006721508
          Length = 569

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   MGAANNI 9
           MGA NNI
Sbjct: 342 MGAMNNI 348



>XP_006721507
          Length = 569

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   MGAANNI 9
           MGA NNI
Sbjct: 342 MGAMNNI 348



>NP_001004725
          Length = 309

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3 MGAANNI 9
         MGA NN+
Sbjct: 1 MGAKNNV 7



>NP_001268421
          Length = 1230

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    TRMGAANNI 9
            TR+GA++ I
Sbjct: 1045 TRLGASDRI 1053



>NP_000170
          Length = 1360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    TRMGAANNI 9
            TR+GA++ I
Sbjct: 1175 TRLGASDRI 1183



>NP_001268422
          Length = 1058

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TRMGAANNI 9
           TR+GA++ I
Sbjct: 873 TRLGASDRI 881



>NP_001268423
          Length = 1058

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TRMGAANNI 9
           TR+GA++ I
Sbjct: 873 TRLGASDRI 881



>NP_001275896
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   TRMGAANN 8
           TR+G  NN
Sbjct: 342 TRLGEMNN 349



>NP_001275895
          Length = 554

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   TRMGAANN 8
           TR+G  NN
Sbjct: 494 TRLGEMNN 501



>NP_001275894
          Length = 577

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   TRMGAANN 8
           TR+G  NN
Sbjct: 474 TRLGEMNN 481



>NP_110409
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  TRMGAANNI 9
          + +GA NNI
Sbjct: 63 SHLGAENNI 71



>NP_077817
          Length = 597

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   TRMGAANN 8
           TR+G  NN
Sbjct: 494 TRLGEMNN 501



>NP_003339
          Length = 152

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TRMGAANNI 9
           TR+ A NNI
Sbjct: 144 TRLYAMNNI 152



>NP_001275893
          Length = 579

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   TRMGAANN 8
           TR+G  NN
Sbjct: 476 TRLGEMNN 483



>NP_001273327
          Length = 542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TRMGAANNI 9
           TR+GAA+ +
Sbjct: 137 TRVGAADGL 145



>XP_006715413
          Length = 464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TRMGAANNI 9
           TR+GAA+ +
Sbjct: 137 TRVGAADGL 145



>XP_006714565
          Length = 565

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  TRMGAANNI 9
          + +GA NNI
Sbjct: 63 SHLGAENNI 71



>XP_005247869
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   TRMGAANN 8
           TR+G  NN
Sbjct: 235 TRLGEMNN 242



>XP_006713830
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   TRMGAANN 8
           TR+G  NN
Sbjct: 318 TRLGEMNN 325



>XP_005247867
          Length = 480

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   TRMGAANN 8
           TR+G  NN
Sbjct: 342 TRLGEMNN 349



>XP_005247866
          Length = 552

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   TRMGAANN 8
           TR+G  NN
Sbjct: 494 TRLGEMNN 501



>XP_005247863
          Length = 597

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   TRMGAANN 8
           TR+G  NN
Sbjct: 494 TRLGEMNN 501



>XP_005247862
          Length = 598

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   TRMGAANN 8
           TR+G  NN
Sbjct: 494 TRLGEMNN 501



>XP_005247861
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   TRMGAANN 8
           TR+G  NN
Sbjct: 474 TRLGEMNN 481



>XP_005247860
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   TRMGAANN 8
           TR+G  NN
Sbjct: 494 TRLGEMNN 501



>XP_005247859
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   TRMGAANN 8
           TR+G  NN
Sbjct: 494 TRLGEMNN 501



>XP_005264328
          Length = 1261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    TRMGAANNI 9
            TR+GA++ I
Sbjct: 1076 TRLGASDRI 1084



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.122    0.331 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 357,095
Number of extensions: 313
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)