Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              20     509
NP_001013679                                                           18    1482
NP_003025                                                              17    3301
XP_005253526                                                           17    3301
NP_667338                                                              17    4312
NP_001159608                                                           17    4312
NP_055452                                                              17    4312
NP_001139311                                                           17    4312
XP_006716547                                                           17    4312
XP_006713923                                                           17    4312
XP_006713922                                                           17    4312
NP_001077368                                                           16    5631
NP_002935                                                              16    5631
NP_061878                                                              16    5631
XP_005249841                                                           16    5631
XP_005249840                                                           16    5631
XP_005249839                                                           16    5631
XP_005249838                                                           16    5631
XP_005249837                                                           16    5631
XP_006715611                                                           16    5631
NP_932349                                                              16    7355
NP_443141                                                              16    7355
NP_694999                                                              16    7355
NP_005671                                                              16    7355
NP_003120                                                              16    7355
XP_006714509                                                           16    7355
XP_005264926                                                           16    7355
NP_002107                                                              15    9605
NP_114434                                                              15    9605
NP_065873                                                              15    9605
NP_004382                                                              15    9605
NP_853514                                                              15    9605
NP_001123986                                                           15    9605
NP_001276903                                                           15    9605
NP_001258932                                                           15    9605
NP_001153880                                                           15    9605
XP_005276335                                                           15    9605
XP_006726487                                                           15    9605
XP_003960167                                                           15    9605
XP_006726110                                                           15    9605
XP_006726109                                                           15    9605
XP_005275388                                                           15    9605
XP_006725814                                                           15    9605
XP_006725813                                                           15    9605
XP_005255320                                                           15    9605
XP_006718411                                                           15    9605
XP_006717257                                                           15    9605
XP_006717256                                                           15    9605
XP_006717062                                                           15    9605
XP_006715687                                                           15    9605
XP_003846269                                                           15    9605
XP_006710241                                                           15    9605
XP_006710240                                                           15    9605
XP_003846670                                                           15    9605
NP_005584                                                              15    9605
NP_072093                                                              15    9605
NP_001229687                                                           15    9605
NP_872369                                                              15    9605
>NP_004295
          Length = 1620

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TSLLWARKS 9
           TSLLWA KS
Sbjct: 887 TSLLWAGKS 895
>NP_001013679
          Length = 385

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LLWARKS 9
           +LWARKS
Sbjct: 312 ILWARKS 318
>NP_003025
          Length = 356

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SLLWARKS 9
           +LLW+RK+
Sbjct: 198 NLLWSRKT 205
>XP_005253526
          Length = 213

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  SLLWARKS 9
          +LLW+RK+
Sbjct: 55 NLLWSRKT 62
>NP_667338
          Length = 1308

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    LLWARK 8
            +LWARK
Sbjct: 1211 MLWARK 1216
>NP_001159608
          Length = 1228

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    LLWARK 8
            +LWARK
Sbjct: 1131 MLWARK 1136
>NP_055452
          Length = 333

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  SLLWARK 8
          S+LW+RK
Sbjct: 22 SVLWSRK 28
>NP_001139311
          Length = 348

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  SLLWARK 8
          S+LW+RK
Sbjct: 22 SVLWSRK 28
>XP_006716547
          Length = 348

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  SLLWARK 8
          S+LW+RK
Sbjct: 22 SVLWSRK 28
>XP_006713923
          Length = 1228

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    LLWARK 8
            +LWARK
Sbjct: 1131 MLWARK 1136
>XP_006713922
          Length = 1262

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    LLWARK 8
            +LWARK
Sbjct: 1211 MLWARK 1216
>NP_001077368
          Length = 620

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TSLLWA 6
           TSL+WA
Sbjct: 445 TSLIWA 450
>NP_002935
          Length = 2347

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   SLLWARKS 9
           +L+WA+K+
Sbjct: 343 TLIWAKKA 350
>NP_061878
          Length = 875

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TSLLWA 6
           TSL+WA
Sbjct: 364 TSLMWA 369
>XP_005249841
          Length = 484

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TSLLWA 6
           TSL+WA
Sbjct: 364 TSLMWA 369
>XP_005249840
          Length = 790

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TSLLWA 6
           TSL+WA
Sbjct: 364 TSLMWA 369
>XP_005249839
          Length = 875

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TSLLWA 6
           TSL+WA
Sbjct: 364 TSLMWA 369
>XP_005249838
          Length = 875

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TSLLWA 6
           TSL+WA
Sbjct: 364 TSLMWA 369
>XP_005249837
          Length = 875

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TSLLWA 6
           TSL+WA
Sbjct: 364 TSLMWA 369
>XP_006715611
          Length = 2342

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   SLLWARKS 9
           +L+WA+K+
Sbjct: 352 TLIWAKKA 359
>NP_932349
          Length = 232

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5  WARKS 9
          WARKS
Sbjct: 12 WARKS 16
>NP_443141
          Length = 1271

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   SLLWARK 8
           SLLW R+
Sbjct: 223 SLLWTRE 229
>NP_694999
          Length = 686

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   SLLWARK 8
           SLLW R+
Sbjct: 610 SLLWRRR 616
>NP_005671
          Length = 588

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SLLWARKS 9
           SLLW R++
Sbjct: 221 SLLWQREA 228
>NP_003120
          Length = 574

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SLLWARKS 9
           S  WARK+
Sbjct: 453 SFYWARKT 460
>XP_006714509
          Length = 1627

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   SLLWARK 8
           SLLW R+
Sbjct: 579 SLLWTRE 585
>XP_005264926
          Length = 251

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   TSLLWAR 7
           TSLLW +
Sbjct: 245 TSLLWCQ 251
>NP_002107
          Length = 365

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   SLLWARKS 9
           +++W RKS
Sbjct: 329 AVMWRRKS 336
>NP_114434
          Length = 492

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   TSLLWAR 7
           TSL+W++
Sbjct: 480 TSLIWSQ 486
>NP_065873
          Length = 1235

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    SLLWARK 8
            SL WAR+
Sbjct: 1109 SLQWARR 1115
>NP_004382
          Length = 501

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   SLLWARK 8
           SLLW R+
Sbjct: 219 SLLWPRE 225
>NP_853514
          Length = 1732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   SLLWARK 8
           +++WARK
Sbjct: 714 TVVWARK 720
>NP_001123986
          Length = 130

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  SLLWARK 8
          SLLW R+
Sbjct: 50 SLLWIRE 56
>NP_001276903
          Length = 579

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   TSLLW 5
           TSLLW
Sbjct: 226 TSLLW 230
>NP_001258932
          Length = 1211

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    SLLWARK 8
            SL WAR+
Sbjct: 1064 SLQWARR 1070
>NP_001153880
          Length = 501

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   TSLLW 5
           TSLLW
Sbjct: 480 TSLLW 484
>XP_005276335
          Length = 356

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1  TSLLW 5
          TSLLW
Sbjct: 54 TSLLW 58

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   TSLLW 5
           TSLLW
Sbjct: 226 TSLLW 230

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   TSLLW 5
           TSLLW
Sbjct: 264 TSLLW 268

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   TSLLW 5
           TSLLW
Sbjct: 302 TSLLW 306
>XP_006726487
          Length = 428

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   TSLLW 5
           TSLLW
Sbjct: 112 TSLLW 116

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   TSLLW 5
           TSLLW
Sbjct: 150 TSLLW 154

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   TSLLW 5
           TSLLW
Sbjct: 284 TSLLW 288
>XP_003960167
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   TSLLW 5
           TSLLW
Sbjct: 309 TSLLW 313
>XP_006726110
          Length = 365

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   SLLWARKS 9
           +++W RKS
Sbjct: 329 AVMWRRKS 336
>XP_006726109
          Length = 394

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   SLLWARKS 9
           +++W RKS
Sbjct: 329 AVMWRRKS 336
>XP_005275388
          Length = 391

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   SLLWARKS 9
           +++W RKS
Sbjct: 326 AVMWRRKS 333
>XP_006725814
          Length = 365

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   SLLWARKS 9
           +++W RKS
Sbjct: 329 AVMWRRKS 336
>XP_006725813
          Length = 394

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   SLLWARKS 9
           +++W RKS
Sbjct: 329 AVMWRRKS 336
>XP_005255320
          Length = 260

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   TSLLWARKS 9
           T + WAR++
Sbjct: 188 TPIYWARRA 196
>XP_006718411
          Length = 319

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  TSLLWAR 7
          +SLLW R
Sbjct: 6  SSLLWPR 12
>XP_006717257
          Length = 1083

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   SLLWARK 8
           SL WAR+
Sbjct: 936 SLQWARR 942
>XP_006717256
          Length = 1172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    SLLWARK 8
            SL WAR+
Sbjct: 1025 SLQWARR 1031
>XP_006717062
          Length = 828

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1 TSLLWARK 8
         T  LW+RK
Sbjct: 2 TVALWSRK 9
>XP_006715687
          Length = 524

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   TSLLW 5
           TSLLW
Sbjct: 150 TSLLW 154

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   TSLLW 5
           TSLLW
Sbjct: 246 TSLLW 250

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   TSLLW 5
           TSLLW
Sbjct: 380 TSLLW 384
>XP_003846269
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   TSLLW 5
           TSLLW
Sbjct: 309 TSLLW 313
>XP_006710241
          Length = 333

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1  TSLLW 5
          TSLLW
Sbjct: 54 TSLLW 58
>XP_006710240
          Length = 372

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1  TSLLW 5
          TSLLW
Sbjct: 93 TSLLW 97
>XP_003846670
          Length = 669

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   TSLLW 5
           TSLLW
Sbjct: 316 TSLLW 320
>NP_005584
          Length = 255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SLLWARKS 9
           S +W+RKS
Sbjct: 172 SPVWSRKS 179
>NP_072093
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   LWARKS 9
           +WARK+
Sbjct: 459 MWARKN 464
>NP_001229687
          Length = 365

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   SLLWARKS 9
           +++W RKS
Sbjct: 329 AVMWRRKS 336
>NP_872369
          Length = 228

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   TSLLWARKS 9
           T + WAR++
Sbjct: 188 TPIYWARRA 196
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.117    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 642,535
Number of extensions: 779
Number of successful extensions: 65
Number of sequences better than 10000.0: 58
Number of HSP's gapped: 65
Number of HSP's successfully gapped: 65
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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