Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004351                                                              20     390
NP_006397                                                              17    2528
XP_005274495                                                           17    2528
NP_001180197                                                           17    4312
NP_036287                                                              17    4312
NP_001180198                                                           17    4312
NP_001257661                                                           17    4312
XP_006723189                                                           17    4312
XP_006723188                                                           17    4312
NP_006784                                                              16    5631
NP_054817                                                              16    5631
NP_060134                                                              16    5631
NP_001240841                                                           16    5631
NP_055988                                                              16    5631
NP_001194005                                                           16    5631
NP_543024                                                              16    5631
NP_001027025                                                           16    5631
XP_006723777                                                           16    5631
XP_005260352                                                           16    5631
XP_005252972                                                           16    5631
XP_006714063                                                           16    5631
XP_005262705                                                           16    5631
XP_006714062                                                           16    5631
XP_005262704                                                           16    5631
XP_005262703                                                           16    5631
NP_001120653                                                           16    7355
NP_001164653                                                           16    7355
NP_201576                                                              16    7355
NP_005036                                                              16    7355
NP_774959                                                              16    7355
NP_932348                                                              16    7355
XP_005256402                                                           16    7355
XP_006722342                                                           16    7355
NP_065095                                                              15    9605
NP_006614                                                              15    9605
NP_001120691                                                           15    9605
NP_115981                                                              15    9605
XP_006718411                                                           15    9605
XP_005253229                                                           15    9605
XP_006718410                                                           15    9605
XP_005253228                                                           15    9605
XP_005253227                                                           15    9605
XP_005246000                                                           15    9605
>NP_004351
          Length = 882

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VEVSKDFGV 9
           VEVS+DFGV
Sbjct: 493 VEVSEDFGV 501
>NP_006397
          Length = 271

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EVSKDFGV 9
           ++SKD+GV
Sbjct: 183 QISKDYGV 190
>XP_005274495
          Length = 257

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EVSKDFGV 9
           ++SKD+GV
Sbjct: 169 QISKDYGV 176
>NP_001180197
          Length = 850

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VEVSKDFG 8
           VEV +DFG
Sbjct: 532 VEVPEDFG 539
>NP_036287
          Length = 649

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VEVSKDFG 8
           VEV +DFG
Sbjct: 331 VEVPEDFG 338
>NP_001180198
          Length = 796

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VEVSKDFG 8
           VEV +DFG
Sbjct: 478 VEVPEDFG 485
>NP_001257661
          Length = 128

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  EVSKDFG 8
          E+S+DFG
Sbjct: 73 EISEDFG 79
>XP_006723189
          Length = 795

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VEVSKDFG 8
           VEV +DFG
Sbjct: 477 VEVPEDFG 484
>XP_006723188
          Length = 849

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VEVSKDFG 8
           VEV +DFG
Sbjct: 531 VEVPEDFG 538
>NP_006784
          Length = 256

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   EVSKDFGV 9
           ++S+D+GV
Sbjct: 167 QISRDYGV 174
>NP_054817
          Length = 238

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   EVSKDFGV 9
           ++S+D+GV
Sbjct: 149 QISRDYGV 156
>NP_060134
          Length = 420

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VEVSKDFGV 9
           +E S+DFGV
Sbjct: 216 LEDSEDFGV 224
>NP_001240841
          Length = 1159

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  EVSKDFGV 9
          EVS DFG+
Sbjct: 16 EVSVDFGL 23
>NP_055988
          Length = 1168

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  EVSKDFGV 9
          EVS DFG+
Sbjct: 16 EVSVDFGL 23
>NP_001194005
          Length = 423

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VSKDFG 8
           +SKDFG
Sbjct: 270 ISKDFG 275
>NP_543024
          Length = 572

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VSKDFG 8
           +SKDFG
Sbjct: 270 ISKDFG 275
>NP_001027025
          Length = 568

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SKDFGV 9
           SKDFG+
Sbjct: 394 SKDFGI 399
>XP_006723777
          Length = 469

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VSKDFG 8
           +SKDFG
Sbjct: 163 ISKDFG 168
>XP_005260352
          Length = 576

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VSKDFG 8
           +SKDFG
Sbjct: 270 ISKDFG 275
>XP_005252972
          Length = 387

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SKDFGV 9
           SKDFG+
Sbjct: 213 SKDFGI 218
>XP_006714063
          Length = 1168

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  EVSKDFGV 9
          EVS DFG+
Sbjct: 16 EVSVDFGL 23
>XP_005262705
          Length = 1181

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  EVSKDFGV 9
          EVS DFG+
Sbjct: 16 EVSVDFGL 23
>XP_006714062
          Length = 1262

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  EVSKDFGV 9
          EVS DFG+
Sbjct: 16 EVSVDFGL 23
>XP_005262704
          Length = 1262

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  EVSKDFGV 9
          EVS DFG+
Sbjct: 16 EVSVDFGL 23
>XP_005262703
          Length = 1275

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  EVSKDFGV 9
          EVS DFG+
Sbjct: 16 EVSVDFGL 23
>NP_001120653
          Length = 390

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VEVSKDFGV 9
           VEV +DF +
Sbjct: 109 VEVGEDFNI 117
>NP_001164653
          Length = 344

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VEVSKDFGV 9
           VEV +DF +
Sbjct: 109 VEVGEDFNI 117
>NP_201576
          Length = 344

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VEVSKDFGV 9
           VEV +DF +
Sbjct: 109 VEVGEDFNI 117
>NP_005036
          Length = 3460

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    VEVSKDFG 8
            +E S+DFG
Sbjct: 2041 LEFSRDFG 2048
>NP_774959
          Length = 3458

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    VEVSKDFG 8
            +E S+DFG
Sbjct: 2041 LEFSRDFG 2048
>NP_932348
          Length = 915

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   EVSKDF 7
           E+SKDF
Sbjct: 511 EISKDF 516
>XP_005256402
          Length = 751

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   EVSKDF 7
           E+SKDF
Sbjct: 511 EISKDF 516
>XP_006722342
          Length = 762

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   EVSKDF 7
           E+SKDF
Sbjct: 522 EISKDF 527
>NP_065095
          Length = 211

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   VEVSKDFG 8
           + +SK+FG
Sbjct: 154 IHISKNFG 161
>NP_006614
          Length = 533

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EVSKDFGV 9
           EVS  FGV
Sbjct: 181 EVSASFGV 188
>NP_001120691
          Length = 501

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SKDFGV 9
           SKDFG+
Sbjct: 322 SKDFGM 327
>NP_115981
          Length = 501

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SKDFGV 9
           SKDFG+
Sbjct: 322 SKDFGM 327
>XP_006718411
          Length = 319

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SKDFGV 9
           SKDFG+
Sbjct: 140 SKDFGM 145
>XP_005253229
          Length = 371

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SKDFGV 9
           SKDFG+
Sbjct: 192 SKDFGM 197
>XP_006718410
          Length = 380

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SKDFGV 9
           SKDFG+
Sbjct: 201 SKDFGM 206
>XP_005253228
          Length = 475

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SKDFGV 9
           SKDFG+
Sbjct: 322 SKDFGM 327
>XP_005253227
          Length = 501

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SKDFGV 9
           SKDFG+
Sbjct: 322 SKDFGM 327
>XP_005246000
          Length = 189

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   VEVSKDFG 8
           + +SK+FG
Sbjct: 154 IHISKNFG 161
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.139    0.369 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 494,745
Number of extensions: 476
Number of successful extensions: 43
Number of sequences better than 10000.0: 43
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 43
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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