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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              18    1482
NP_001035957                                                           18    1482
XP_006721991                                                           18    1482
XP_006721990                                                           18    1482
XP_006721989                                                           18    1482
XP_005258041                                                           18    1482
XP_006721988                                                           18    1482
XP_006721987                                                           18    1482
XP_006721986                                                           18    1482
XP_005258040                                                           18    1482
XP_006721985                                                           18    1482
NP_001162625                                                           16    7355
NP_775765                                                              16    7355
NP_001248755                                                           16    7355
NP_003929                                                              16    7355
XP_006722995                                                           16    7355
NP_055248                                                              16    7355
NP_001243465                                                           15    9605
NP_062828                                                              15    9605
XP_006719049                                                           15    9605

>NP_000258
          Length = 2818

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VLAMSKLLN 9
            VLAMS LLN
Sbjct: 1146 VLAMSNLLN 1154



>NP_001035957
          Length = 2839

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VLAMSKLLN 9
            VLAMS LLN
Sbjct: 1146 VLAMSNLLN 1154



>XP_006721991
          Length = 1632

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VLAMSKLLN 9
            VLAMS LLN
Sbjct: 1156 VLAMSNLLN 1164



>XP_006721990
          Length = 2823

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VLAMSKLLN 9
            VLAMS LLN
Sbjct: 1156 VLAMSNLLN 1164



>XP_006721989
          Length = 2823

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VLAMSKLLN 9
            VLAMS LLN
Sbjct: 1156 VLAMSNLLN 1164



>XP_005258041
          Length = 2836

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VLAMSKLLN 9
            VLAMS LLN
Sbjct: 1146 VLAMSNLLN 1154



>XP_006721988
          Length = 2846

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VLAMSKLLN 9
            VLAMS LLN
Sbjct: 1156 VLAMSNLLN 1164



>XP_006721987
          Length = 2849

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VLAMSKLLN 9
            VLAMS LLN
Sbjct: 1156 VLAMSNLLN 1164



>XP_006721986
          Length = 2854

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VLAMSKLLN 9
            VLAMS LLN
Sbjct: 1143 VLAMSNLLN 1151



>XP_005258040
          Length = 2857

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VLAMSKLLN 9
            VLAMS LLN
Sbjct: 1146 VLAMSNLLN 1154



>XP_006721985
          Length = 2867

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VLAMSKLLN 9
            VLAMS LLN
Sbjct: 1156 VLAMSNLLN 1164



>NP_001162625
          Length = 214

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  AMSKLLN 9
          A+SKLLN
Sbjct: 34 ALSKLLN 40



>NP_775765
          Length = 214

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  AMSKLLN 9
          A+SKLLN
Sbjct: 34 ALSKLLN 40



>NP_001248755
          Length = 1215

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   VLAMSKLL 8
           +LAMSK+L
Sbjct: 324 LLAMSKIL 331



>NP_003929
          Length = 1153

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   VLAMSKLL 8
           +LAMSK+L
Sbjct: 324 LLAMSKIL 331



>XP_006722995
          Length = 1203

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   VLAMSKLL 8
           +LAMSK+L
Sbjct: 324 LLAMSKIL 331



>NP_055248
          Length = 348

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   VLAMSKLLN 9
           VLA+S LLN
Sbjct: 110 VLALSFLLN 118



>NP_001243465
          Length = 385

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  MSKLLN 9
          MSKLLN
Sbjct: 52 MSKLLN 57



>NP_062828
          Length = 394

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  MSKLLN 9
          MSKLLN
Sbjct: 61 MSKLLN 66



>XP_006719049
          Length = 257

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4 MSKLLN 9
         MSKLLN
Sbjct: 1 MSKLLN 6



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.132    0.308 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 231,851
Number of extensions: 202
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)