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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     869
NP_001075109                                                           19     869
NP_001361                                                              17    2528
XP_006712019                                                           17    2528
NP_001274691                                                           16    5631
NP_001274690                                                           16    5631
NP_004204                                                              16    5631
XP_005255050                                                           16    5631
XP_005255048                                                           16    5631
XP_005255046                                                           16    5631
NP_001135828                                                           15    9605
NP_085150                                                              15    9605
NP_689824                                                              15    9605
XP_005257331                                                           15    9605
XP_006712739                                                           15    9605

>NP_008835
          Length = 4128

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VLRAIFTSE 9
            VLR IFTSE
Sbjct: 2938 VLRGIFTSE 2946



>NP_001075109
          Length = 4097

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VLRAIFTSE 9
            VLR IFTSE
Sbjct: 2938 VLRGIFTSE 2946



>NP_001361
          Length = 4158

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VLRAIFTSE 9
            +LR +FTSE
Sbjct: 3239 ILRMLFTSE 3247



>XP_006712019
          Length = 4158

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VLRAIFTSE 9
            +LR +FTSE
Sbjct: 3239 ILRMLFTSE 3247



>NP_001274691
          Length = 649

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VLRAIFT 7
           VLRA+FT
Sbjct: 569 VLRALFT 575



>NP_001274690
          Length = 483

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VLRAIFT 7
           VLRA+FT
Sbjct: 403 VLRALFT 409



>NP_004204
          Length = 649

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VLRAIFT 7
           VLRA+FT
Sbjct: 569 VLRALFT 575



>XP_005255050
          Length = 623

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VLRAIFT 7
           VLRA+FT
Sbjct: 543 VLRALFT 549



>XP_005255048
          Length = 649

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VLRAIFT 7
           VLRA+FT
Sbjct: 569 VLRALFT 575



>XP_005255046
          Length = 649

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VLRAIFT 7
           VLRA+FT
Sbjct: 569 VLRALFT 575



>NP_001135828
          Length = 285

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VLRAIFTSE 9
           VLR  FTS+
Sbjct: 262 VLRLTFTSQ 270



>NP_085150
          Length = 941

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LRAIFTSE 9
           L++IFT+E
Sbjct: 672 LKSIFTTE 679



>NP_689824
          Length = 740

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VLRAIFTSE 9
          +LRA+ TSE
Sbjct: 86 LLRALSTSE 94



>XP_005257331
          Length = 554

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  LRAIFTS 8
          +RA+FTS
Sbjct: 10 IRALFTS 16



>XP_006712739
          Length = 285

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VLRAIFTSE 9
           VLR  FTS+
Sbjct: 262 VLRLTFTSQ 270



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.332    0.139    0.344 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 301,701
Number of extensions: 381
Number of successful extensions: 15
Number of sequences better than 10000.0: 15
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)