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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005179                                                              20     509
NP_002935                                                              17    2528
XP_006715611                                                           17    2528
NP_000426                                                              16    5631
NP_722575                                                              16    5631
NP_620130                                                              16    5631
XP_005259981                                                           16    5631
XP_006719005                                                           16    5631
XP_005271789                                                           16    5631
XP_005271787                                                           16    5631
XP_005271786                                                           16    5631
XP_005271785                                                           16    5631
XP_005271784                                                           16    5631
XP_005247180                                                           16    5631
NP_112181                                                              16    7355
NP_060417                                                              15    9605

>NP_005179
          Length = 906

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VPAMLARRT 9
           VPA+LARRT
Sbjct: 781 VPAVLARRT 789



>NP_002935
          Length = 2347

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    VPAMLARRT 9
            VPAMLA +T
Sbjct: 1376 VPAMLAGKT 1384



>XP_006715611
          Length = 2342

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    VPAMLARRT 9
            VPAMLA +T
Sbjct: 1371 VPAMLAGKT 1379



>NP_000426
          Length = 2321

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VPAMLAR 7
            VPA+LAR
Sbjct: 1504 VPALLAR 1510



>NP_722575
          Length = 346

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VPAMLARRT 9
           VPA L+R+T
Sbjct: 311 VPAHLSRKT 319



>NP_620130
          Length = 664

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VPAMLARRT 9
           VP ML RR+
Sbjct: 270 VPVMLPRRS 278



>XP_005259981
          Length = 2269

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VPAMLAR 7
            VPA+LAR
Sbjct: 1452 VPALLAR 1458



>XP_006719005
          Length = 348

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VPAMLARRT 9
           VP ML RR+
Sbjct: 273 VPVMLPRRS 281



>XP_005271789
          Length = 349

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VPAMLARRT 9
           VP ML RR+
Sbjct: 273 VPVMLPRRS 281



>XP_005271787
          Length = 412

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  VPAMLARRT 9
          VP ML RR+
Sbjct: 18 VPVMLPRRS 26



>XP_005271786
          Length = 527

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VPAMLARRT 9
           VP ML RR+
Sbjct: 133 VPVMLPRRS 141



>XP_005271785
          Length = 527

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VPAMLARRT 9
           VP ML RR+
Sbjct: 133 VPVMLPRRS 141



>XP_005271784
          Length = 667

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VPAMLARRT 9
           VP ML RR+
Sbjct: 273 VPVMLPRRS 281



>XP_005247180
          Length = 207

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VPAMLARRT 9
           VPA L+R+T
Sbjct: 172 VPAHLSRKT 180



>NP_112181
          Length = 615

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AMLARRT 9
           AML+RRT
Sbjct: 577 AMLSRRT 583



>NP_060417
          Length = 888

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VPAMLARRT 9
           +PA++ RR+
Sbjct: 520 IPAVMGRRS 528



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.132    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 398,558
Number of extensions: 613
Number of successful extensions: 16
Number of sequences better than 10000.0: 16
Number of HSP's gapped: 16
Number of HSP's successfully gapped: 16
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)