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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001156685                                                           18    1135
NP_000133                                                              18    1135
NP_075254                                                              18    1135
XP_006713936                                                           18    1135
XP_006713935                                                           18    1135
XP_006713934                                                           18    1135
XP_006713933                                                           18    1135
XP_006713932                                                           18    1135
XP_006713931                                                           18    1135
NP_005286                                                              17    3301
NP_814444                                                              16    7355
NP_004596                                                              16    7355
XP_005259239                                                           16    7355
XP_006716001                                                           16    7355
NP_000836                                                              16    7355

>NP_001156685
          Length = 808

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VSLESNTSM 9
           VSLESN SM
Sbjct: 425 VSLESNASM 433



>NP_000133
          Length = 806

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VSLESNTSM 9
           VSLESN SM
Sbjct: 423 VSLESNASM 431



>NP_075254
          Length = 694

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VSLESNTSM 9
           VSLESN SM
Sbjct: 311 VSLESNASM 319



>XP_006713936
          Length = 807

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VSLESNTSM 9
           VSLESN SM
Sbjct: 423 VSLESNASM 431



>XP_006713935
          Length = 807

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VSLESNTSM 9
           VSLESN SM
Sbjct: 424 VSLESNASM 432



>XP_006713934
          Length = 809

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VSLESNTSM 9
           VSLESN SM
Sbjct: 425 VSLESNASM 433



>XP_006713933
          Length = 810

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VSLESNTSM 9
           VSLESN SM
Sbjct: 426 VSLESNASM 434



>XP_006713932
          Length = 810

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VSLESNTSM 9
           VSLESN SM
Sbjct: 427 VSLESNASM 435



>XP_006713931
          Length = 811

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VSLESNTSM 9
           VSLESN SM
Sbjct: 427 VSLESNASM 435



>NP_005286
          Length = 433

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   VSLESNTSM 9
           +SLESNT++
Sbjct: 105 LSLESNTAL 113



>NP_814444
          Length = 365

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SLESNTSM 9
           SLE NTS+
Sbjct: 332 SLEPNTSL 339



>NP_004596
          Length = 350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SLESNTSM 9
           SLE NTS+
Sbjct: 317 SLEPNTSL 324



>XP_005259239
          Length = 221

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SLESNTSM 9
           SLE NTS+
Sbjct: 188 SLEPNTSL 195



>XP_006716001
          Length = 908

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SLESNTS 8
           SLE+NTS
Sbjct: 888 SLETNTS 894



>NP_000836
          Length = 908

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SLESNTS 8
           SLE+NTS
Sbjct: 888 SLETNTS 894



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.287   0.0985    0.211 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 351,100
Number of extensions: 355
Number of successful extensions: 15
Number of sequences better than 10000.0: 15
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.9 bits)
S2: 28 (15.4 bits)