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    ELISA

    Enzyme-Linked Immunosorbent Assay (ELISA) is a widely-used method for measuring the concentration of a particular molecule (e.g., a hormone or drug) in a fluid such as serum or urine. It is also known as enzyme immunoassay or EIA.

    The molecule is detected by antibodies that have been made against it; that is, for which it is the antigen. Monoclonal antibodies are often used.

    The test requires:
  • The antibodies fixed to a solid surface, such as the inner surface of a test tube;
  • A preparation of the same antibodies coupled to enzyme an one (e.g., --galactosidase) that produces a colored product from a colorless substrate.

  • ELISA protocol