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    Recombinant Antibodies

    A Recombinant Antibody is defined as an antibody produced by genetic engineering. Recombinant antibody has significant advantages compared with the conventional antibody and there for its use becoming more popular now days. The fact that no animals are needed in the manufacturing procedure of the recombinant antibodies, in addition, the manufacturing time is relatively short compared with the conventional method. Moreover, the quality of the final product is higher that these of the non recombinant method.

    What are recombinant antibodies ?

    The term recombinant and especially recombinant DNA regards to an artificial methods of producing DNA (synthetic DNA and proteins).
    The manufacturing technique make use of bacterias, these bacterias are genetically modified in such a way that a specific DNA segment (gene) is artificially combined with their original DNA. After this insertion of DNA segment. The bacterias are nourished and kept in ideal conditions for reproduction and after a relative short period of time, after the reproduction, these specific segment of DNA is extracted out of the original DNA bacterias.
    In short a segment of DNA is recombined (recombinant) with the original DNA of the bacteria, these genetically modified bacterias are rapidly reproduce and by that reproduce the DNA segment that was recombined with the original genome.
    Using recombinant antibody has significant advantages compared with the conventional antibody and there for its use becoming more popular now days. The fact that no animals are needed in the manufacturing procedure of the recombinant antibodies, in addition, the manufacturing time is relatively short compared with the conventional method. Moreover, the quality of the final product is higher that these of the non recombinant method.

    Production of recombinant antibodies

    The production of non-animal recombinant antibodies can be broken down into five steps:
  • (1) creation of an antibody gene library
  • (2) display of the library on phage coats or cell surfaces
  • (3) isolation of antibodies against an antigen of interest
  • (4) modification of the isolated antibodies and
  • (5) scaled up production of selected antibodies in a cell culture expression system.
    Detailed protocol

    Uses of recombinant antibodies

    These include:
  • diagnostic test that discriminates between closely related pathogens or antigens
  • production of binders to difficult agents especially in areas where conventional antibodies often fail
  • highly pathogenic agents and emerging diseases
  • conserved mammalian epitopes
  • small and less immunogenic haptens
  • detection of non-protein moieties, for example, toxins
  • range of highly specific reagents for use in multiplex assays.
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