Genome Annotation

We have annotated the 8.23-Mb draft genome of strain Rhodococcus imtechensis RKJ300 by PGAAP pipeline and RNAmmer 1.2 server.
Annotated genome is available at NCBI as accession number AJJH00000000.
A total of 7,681 protein coding genes (i.e. CDS), 5 rRNAs and 50 tRNAs genes have been predicted after the annotation. Out of 7,681 genes, 4,847 genes were assigned the function and 2,834 remain as hypothetical genes.


Genome Annotation Statistics

Measure Value
Total size(bp) 8,231,340
Protein coding genes7,681
Function assigned genes4,847
Hypothetical genes2,834
tRNA predicted 50
rRNA predicted 50
GC Content (%) 67.22

We have adopted several strategies to further annotate the genome of Rhodococcus imtechensis RKJ300.

  • Gene prediction and annotation by PGAAP pipeline and RNAmmer server.
  • Mapping of proteins to KEGG Pathways.
  • Phylogenomics analysis.
  • Whole genome comparison of Rhodococcus sp. M213, Rhodococcus imtechensis RKJ300 with closest strains, Rhodococcus opacus PD630, Rhodococcus jostii RHA1 and Rhodococcus sp. JVH1.
  • Identification of genes involved in the biodegradation of Benzoate, Chloroalkane and Chloroalkene, Chlorocyclohexane and Chlorobenzene, Toluene, Xylene, Dioxin, Naphthalene, Fluorobenzoate, Ethyl benzene, Nitrotoluene and Limonene and Pinene etc.
  • Identification of genes involved in the biodegradation of Phthalates (PTH) and Phthalate Esters (PEs), Vanillin, Cholate and Steroids.
  • Identification of genes involved in the metabolism of triacylglycerols (TAG), wax esters, polyhydroxyalkanoates (PHA), glycogen and polyphosphate (PolyP).
  • Identification and comparison of Phthalate and Terephthalate, Vanillin, and Steroid degradation gene clusters in compared strains i.e. Rhodococcus imtechensis RKJ300 with closest strains, Rhodococcus sp. M213, Rhodococcus opacus PD630, Rhodococcus jostii RHA1 and Rhodococcus sp. JVH1.