Rhodococcus imtechensis RKJ300

Genome Sequencing

Whole genome of Rhodococcus imtechensis RKJ300 was sequenced by Illumina-HiSeq 1000 paired end technology. A total of 30,464,548 paired end reads of 101 bp length were generated as a result of sequencing.
We have used NGS QC Toolkit v2.1 to filter high-quality Illumina data (HQ cut off read length for HQ=70%, cutoff quality score=20) and removal of Vector/Adaptor contaminated reads.
We obtained 2,753,155 single end reads and 22,040,838 high-quality, vector-filtered paired end reads after raw data filtering.
We have only used 22,040,838 paired end reads,having ~267X coverage, for genome assembly, discussed in the genome annocement manuscript.

Quality filtering statistics

Illumina Reads	Forward Reads	Reversed reads
Total number of raw reads	15232274	15232274
Total number of HQ reads	11020437	11020437
Percentage of HQ reads	72.35%	72.35%
Total number of bases	1538459674	1538459674
Total number of bases in HQ reads	1113064137	1113064137
Total number of HQ bases in HQ reads	1028610488	1018508024
Percentage of HQ bases in HQ reads	92.41%	91.50%
Number of Primer/Adaptor contaminated HQ reads	12	6
Total number of HQ filtered reads	11020419	11020419
Percentage of HQ filtered reads	72.35%	72.35%
Total number of High Quality, Vector filtered single end reads	2,753,155
Total number of High Quality, Vector filtered paired end reads	22,040,838

Rhodococcus imtechensis RKJ300

Genome Sequencing

Quality filtering statistics

ABOUT

Genome Annotation

Genome BLAST

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